Amino acid dipepetide frequency for Gordonia phage Mariokart

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.484AlaAla: 16.484 ± 1.095
0.735AlaCys: 0.735 ± 0.214
9.974AlaAsp: 9.974 ± 0.918
8.347AlaGlu: 8.347 ± 0.944
2.835AlaPhe: 2.835 ± 0.543
9.134AlaGly: 9.134 ± 0.812
2.257AlaHis: 2.257 ± 0.441
4.987AlaIle: 4.987 ± 0.538
4.305AlaLys: 4.305 ± 0.619
9.187AlaLeu: 9.187 ± 0.683
3.412AlaMet: 3.412 ± 0.583
2.52AlaAsn: 2.52 ± 0.334
6.772AlaPro: 6.772 ± 0.577
4.095AlaGln: 4.095 ± 0.468
8.084AlaArg: 8.084 ± 0.746
6.615AlaSer: 6.615 ± 0.557
7.927AlaThr: 7.927 ± 0.64
11.969AlaVal: 11.969 ± 0.836
1.89AlaTrp: 1.89 ± 0.336
2.94AlaTyr: 2.94 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.682CysAla: 0.682 ± 0.191
0.105CysCys: 0.105 ± 0.074
0.997CysAsp: 0.997 ± 0.411
0.63CysGlu: 0.63 ± 0.188
0.157CysPhe: 0.157 ± 0.102
1.47CysGly: 1.47 ± 0.398
0.262CysHis: 0.262 ± 0.109
0.105CysIle: 0.105 ± 0.079
0.105CysLys: 0.105 ± 0.075
0.315CysLeu: 0.315 ± 0.13
0.21CysMet: 0.21 ± 0.131
0.367CysAsn: 0.367 ± 0.156
0.84CysPro: 0.84 ± 0.259
0.21CysGln: 0.21 ± 0.111
0.787CysArg: 0.787 ± 0.231
0.63CysSer: 0.63 ± 0.182
0.367CysThr: 0.367 ± 0.13
0.787CysVal: 0.787 ± 0.176
0.105CysTrp: 0.105 ± 0.078
0.262CysTyr: 0.262 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
8.977AspAla: 8.977 ± 0.794
0.682AspCys: 0.682 ± 0.165
5.67AspAsp: 5.67 ± 0.741
5.775AspGlu: 5.775 ± 0.628
1.575AspPhe: 1.575 ± 0.231
6.3AspGly: 6.3 ± 0.643
1.575AspHis: 1.575 ± 0.334
2.362AspIle: 2.362 ± 0.325
1.102AspLys: 1.102 ± 0.288
7.559AspLeu: 7.559 ± 0.621
1.837AspMet: 1.837 ± 0.316
1.89AspAsn: 1.89 ± 0.313
4.515AspPro: 4.515 ± 0.61
2.152AspGln: 2.152 ± 0.448
4.83AspArg: 4.83 ± 0.501
3.622AspSer: 3.622 ± 0.469
4.567AspThr: 4.567 ± 0.46
3.78AspVal: 3.78 ± 0.45
1.575AspTrp: 1.575 ± 0.363
1.47AspTyr: 1.47 ± 0.281
0.0AspXaa: 0.0 ± 0.0
Glu
6.982GluAla: 6.982 ± 0.632
0.63GluCys: 0.63 ± 0.172
3.885GluAsp: 3.885 ± 0.558
3.097GluGlu: 3.097 ± 0.427
1.575GluPhe: 1.575 ± 0.266
3.99GluGly: 3.99 ± 0.543
1.26GluHis: 1.26 ± 0.223
4.095GluIle: 4.095 ± 0.515
1.68GluLys: 1.68 ± 0.325
5.827GluLeu: 5.827 ± 0.624
1.732GluMet: 1.732 ± 0.29
1.312GluAsn: 1.312 ± 0.275
2.572GluPro: 2.572 ± 0.444
2.362GluGln: 2.362 ± 0.349
3.99GluArg: 3.99 ± 0.487
2.152GluSer: 2.152 ± 0.363
3.937GluThr: 3.937 ± 0.471
5.25GluVal: 5.25 ± 0.472
0.997GluTrp: 0.997 ± 0.235
1.207GluTyr: 1.207 ± 0.251
0.0GluXaa: 0.0 ± 0.0
Phe
2.94PheAla: 2.94 ± 0.362
0.262PheCys: 0.262 ± 0.115
2.782PheAsp: 2.782 ± 0.397
1.417PheGlu: 1.417 ± 0.294
0.472PhePhe: 0.472 ± 0.196
2.257PheGly: 2.257 ± 0.354
0.42PheHis: 0.42 ± 0.141
1.155PheIle: 1.155 ± 0.237
0.472PheLys: 0.472 ± 0.146
1.312PheLeu: 1.312 ± 0.227
0.472PheMet: 0.472 ± 0.159
0.63PheAsn: 0.63 ± 0.153
0.63PhePro: 0.63 ± 0.157
0.577PheGln: 0.577 ± 0.174
1.785PheArg: 1.785 ± 0.3
1.26PheSer: 1.26 ± 0.235
2.205PheThr: 2.205 ± 0.373
1.837PheVal: 1.837 ± 0.29
0.21PheTrp: 0.21 ± 0.102
0.63PheTyr: 0.63 ± 0.175
0.0PheXaa: 0.0 ± 0.0
Gly
9.869GlyAla: 9.869 ± 0.734
0.735GlyCys: 0.735 ± 0.217
5.512GlyAsp: 5.512 ± 0.455
4.252GlyGlu: 4.252 ± 0.44
2.362GlyPhe: 2.362 ± 0.389
7.664GlyGly: 7.664 ± 1.182
1.732GlyHis: 1.732 ± 0.297
3.78GlyIle: 3.78 ± 0.635
2.94GlyLys: 2.94 ± 0.388
8.084GlyLeu: 8.084 ± 0.745
1.732GlyMet: 1.732 ± 0.329
2.047GlyAsn: 2.047 ± 0.351
3.097GlyPro: 3.097 ± 0.421
2.992GlyGln: 2.992 ± 0.3
6.247GlyArg: 6.247 ± 0.583
5.827GlySer: 5.827 ± 0.667
5.827GlyThr: 5.827 ± 0.658
6.405GlyVal: 6.405 ± 0.498
2.52GlyTrp: 2.52 ± 0.366
2.73GlyTyr: 2.73 ± 0.382
0.0GlyXaa: 0.0 ± 0.0
His
1.89HisAla: 1.89 ± 0.384
0.21HisCys: 0.21 ± 0.094
1.522HisAsp: 1.522 ± 0.324
1.155HisGlu: 1.155 ± 0.249
0.525HisPhe: 0.525 ± 0.183
1.207HisGly: 1.207 ± 0.273
0.735HisHis: 0.735 ± 0.182
0.525HisIle: 0.525 ± 0.137
0.315HisLys: 0.315 ± 0.114
2.047HisLeu: 2.047 ± 0.373
0.157HisMet: 0.157 ± 0.098
0.577HisAsn: 0.577 ± 0.202
1.155HisPro: 1.155 ± 0.263
0.525HisGln: 0.525 ± 0.174
1.312HisArg: 1.312 ± 0.258
0.787HisSer: 0.787 ± 0.255
1.417HisThr: 1.417 ± 0.253
1.05HisVal: 1.05 ± 0.231
0.577HisTrp: 0.577 ± 0.141
0.525HisTyr: 0.525 ± 0.162
0.0HisXaa: 0.0 ± 0.0
Ile
7.402IleAla: 7.402 ± 0.753
0.42IleCys: 0.42 ± 0.141
3.78IleAsp: 3.78 ± 0.398
4.147IleGlu: 4.147 ± 0.518
0.525IlePhe: 0.525 ± 0.155
4.672IleGly: 4.672 ± 0.761
0.63IleHis: 0.63 ± 0.177
1.995IleIle: 1.995 ± 0.334
1.312IleLys: 1.312 ± 0.378
2.047IleLeu: 2.047 ± 0.273
0.682IleMet: 0.682 ± 0.166
1.26IleAsn: 1.26 ± 0.32
2.047IlePro: 2.047 ± 0.339
1.26IleGln: 1.26 ± 0.313
2.047IleArg: 2.047 ± 0.362
2.415IleSer: 2.415 ± 0.323
3.255IleThr: 3.255 ± 0.505
3.045IleVal: 3.045 ± 0.376
0.472IleTrp: 0.472 ± 0.173
0.892IleTyr: 0.892 ± 0.199
0.0IleXaa: 0.0 ± 0.0
Lys
3.622LysAla: 3.622 ± 0.402
0.262LysCys: 0.262 ± 0.124
1.102LysAsp: 1.102 ± 0.25
0.577LysGlu: 0.577 ± 0.163
0.735LysPhe: 0.735 ± 0.205
2.467LysGly: 2.467 ± 0.419
0.21LysHis: 0.21 ± 0.103
1.627LysIle: 1.627 ± 0.369
0.367LysLys: 0.367 ± 0.163
2.205LysLeu: 2.205 ± 0.406
0.315LysMet: 0.315 ± 0.147
0.63LysAsn: 0.63 ± 0.157
1.732LysPro: 1.732 ± 0.407
0.682LysGln: 0.682 ± 0.179
1.995LysArg: 1.995 ± 0.373
1.365LysSer: 1.365 ± 0.256
1.995LysThr: 1.995 ± 0.298
2.415LysVal: 2.415 ± 0.374
0.262LysTrp: 0.262 ± 0.125
0.42LysTyr: 0.42 ± 0.12
0.0LysXaa: 0.0 ± 0.0
Leu
11.129LeuAla: 11.129 ± 0.923
0.63LeuCys: 0.63 ± 0.194
7.087LeuAsp: 7.087 ± 0.588
2.887LeuGlu: 2.887 ± 0.314
1.522LeuPhe: 1.522 ± 0.275
7.717LeuGly: 7.717 ± 0.715
1.522LeuHis: 1.522 ± 0.314
3.36LeuIle: 3.36 ± 0.427
1.732LeuLys: 1.732 ± 0.383
4.62LeuLeu: 4.62 ± 0.45
2.047LeuMet: 2.047 ± 0.317
2.152LeuAsn: 2.152 ± 0.388
4.777LeuPro: 4.777 ± 0.574
2.677LeuGln: 2.677 ± 0.346
5.407LeuArg: 5.407 ± 0.63
4.095LeuSer: 4.095 ± 0.416
6.562LeuThr: 6.562 ± 0.474
5.67LeuVal: 5.67 ± 0.534
1.995LeuTrp: 1.995 ± 0.311
1.89LeuTyr: 1.89 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
2.94MetAla: 2.94 ± 0.346
0.262MetCys: 0.262 ± 0.14
1.102MetAsp: 1.102 ± 0.269
0.997MetGlu: 0.997 ± 0.202
0.892MetPhe: 0.892 ± 0.24
1.837MetGly: 1.837 ± 0.299
0.42MetHis: 0.42 ± 0.197
1.365MetIle: 1.365 ± 0.254
0.577MetLys: 0.577 ± 0.167
1.68MetLeu: 1.68 ± 0.34
0.577MetMet: 0.577 ± 0.19
0.315MetAsn: 0.315 ± 0.148
1.417MetPro: 1.417 ± 0.237
0.682MetGln: 0.682 ± 0.202
2.152MetArg: 2.152 ± 0.303
2.1MetSer: 2.1 ± 0.284
2.625MetThr: 2.625 ± 0.339
1.89MetVal: 1.89 ± 0.291
0.42MetTrp: 0.42 ± 0.148
0.42MetTyr: 0.42 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
3.412AsnAla: 3.412 ± 0.386
0.42AsnCys: 0.42 ± 0.172
1.47AsnAsp: 1.47 ± 0.261
1.155AsnGlu: 1.155 ± 0.274
0.262AsnPhe: 0.262 ± 0.114
2.94AsnGly: 2.94 ± 0.552
0.42AsnHis: 0.42 ± 0.139
0.525AsnIle: 0.525 ± 0.164
0.42AsnLys: 0.42 ± 0.187
1.732AsnLeu: 1.732 ± 0.353
0.367AsnMet: 0.367 ± 0.152
1.207AsnAsn: 1.207 ± 0.334
1.522AsnPro: 1.522 ± 0.281
0.997AsnGln: 0.997 ± 0.23
1.575AsnArg: 1.575 ± 0.262
1.732AsnSer: 1.732 ± 0.227
1.837AsnThr: 1.837 ± 0.267
2.52AsnVal: 2.52 ± 0.511
0.63AsnTrp: 0.63 ± 0.205
0.682AsnTyr: 0.682 ± 0.185
0.0AsnXaa: 0.0 ± 0.0
Pro
5.775ProAla: 5.775 ± 0.561
0.42ProCys: 0.42 ± 0.201
4.935ProAsp: 4.935 ± 0.602
4.357ProGlu: 4.357 ± 0.462
1.89ProPhe: 1.89 ± 0.304
4.41ProGly: 4.41 ± 0.482
0.682ProHis: 0.682 ± 0.24
2.152ProIle: 2.152 ± 0.265
0.945ProLys: 0.945 ± 0.244
3.57ProLeu: 3.57 ± 0.443
1.26ProMet: 1.26 ± 0.26
1.155ProAsn: 1.155 ± 0.243
3.307ProPro: 3.307 ± 0.543
1.942ProGln: 1.942 ± 0.339
2.677ProArg: 2.677 ± 0.361
3.622ProSer: 3.622 ± 0.527
4.095ProThr: 4.095 ± 0.454
5.775ProVal: 5.775 ± 0.608
1.155ProTrp: 1.155 ± 0.286
1.05ProTyr: 1.05 ± 0.228
0.0ProXaa: 0.0 ± 0.0
Gln
4.62GlnAla: 4.62 ± 0.375
0.21GlnCys: 0.21 ± 0.097
0.997GlnAsp: 0.997 ± 0.254
1.365GlnGlu: 1.365 ± 0.324
0.682GlnPhe: 0.682 ± 0.197
2.362GlnGly: 2.362 ± 0.39
0.472GlnHis: 0.472 ± 0.165
1.732GlnIle: 1.732 ± 0.335
0.472GlnLys: 0.472 ± 0.167
3.412GlnLeu: 3.412 ± 0.412
0.892GlnMet: 0.892 ± 0.255
0.945GlnAsn: 0.945 ± 0.233
1.26GlnPro: 1.26 ± 0.222
1.312GlnGln: 1.312 ± 0.29
1.942GlnArg: 1.942 ± 0.388
2.1GlnSer: 2.1 ± 0.333
2.205GlnThr: 2.205 ± 0.379
3.045GlnVal: 3.045 ± 0.483
0.682GlnTrp: 0.682 ± 0.161
0.577GlnTyr: 0.577 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
8.032ArgAla: 8.032 ± 0.605
0.577ArgCys: 0.577 ± 0.151
4.62ArgAsp: 4.62 ± 0.477
4.305ArgGlu: 4.305 ± 0.487
1.627ArgPhe: 1.627 ± 0.324
4.147ArgGly: 4.147 ± 0.365
1.627ArgHis: 1.627 ± 0.296
2.467ArgIle: 2.467 ± 0.4
1.732ArgLys: 1.732 ± 0.297
5.985ArgLeu: 5.985 ± 0.472
1.995ArgMet: 1.995 ± 0.348
1.575ArgAsn: 1.575 ± 0.261
3.832ArgPro: 3.832 ± 0.534
2.205ArgGln: 2.205 ± 0.383
5.67ArgArg: 5.67 ± 0.756
3.885ArgSer: 3.885 ± 0.544
4.252ArgThr: 4.252 ± 0.486
5.145ArgVal: 5.145 ± 0.615
1.522ArgTrp: 1.522 ± 0.304
2.31ArgTyr: 2.31 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
6.51SerAla: 6.51 ± 0.681
0.577SerCys: 0.577 ± 0.229
3.15SerAsp: 3.15 ± 0.375
2.205SerGlu: 2.205 ± 0.285
1.312SerPhe: 1.312 ± 0.257
6.247SerGly: 6.247 ± 0.821
0.997SerHis: 0.997 ± 0.242
3.15SerIle: 3.15 ± 0.406
1.365SerLys: 1.365 ± 0.271
4.2SerLeu: 4.2 ± 0.558
1.995SerMet: 1.995 ± 0.313
1.417SerAsn: 1.417 ± 0.255
3.727SerPro: 3.727 ± 0.504
1.785SerGln: 1.785 ± 0.252
3.832SerArg: 3.832 ± 0.447
2.887SerSer: 2.887 ± 0.455
4.2SerThr: 4.2 ± 0.502
4.725SerVal: 4.725 ± 0.633
1.26SerTrp: 1.26 ± 0.191
1.417SerTyr: 1.417 ± 0.305
0.0SerXaa: 0.0 ± 0.0
Thr
9.712ThrAla: 9.712 ± 0.713
0.735ThrCys: 0.735 ± 0.273
4.41ThrAsp: 4.41 ± 0.537
4.095ThrGlu: 4.095 ± 0.507
1.417ThrPhe: 1.417 ± 0.286
6.3ThrGly: 6.3 ± 0.571
0.945ThrHis: 0.945 ± 0.295
3.517ThrIle: 3.517 ± 0.397
2.047ThrLys: 2.047 ± 0.288
5.722ThrLeu: 5.722 ± 0.53
1.837ThrMet: 1.837 ± 0.365
1.68ThrAsn: 1.68 ± 0.323
4.987ThrPro: 4.987 ± 0.619
1.89ThrGln: 1.89 ± 0.369
4.777ThrArg: 4.777 ± 0.473
3.727ThrSer: 3.727 ± 0.566
5.355ThrThr: 5.355 ± 0.853
5.355ThrVal: 5.355 ± 0.479
1.575ThrTrp: 1.575 ± 0.23
1.785ThrTyr: 1.785 ± 0.301
0.0ThrXaa: 0.0 ± 0.0
Val
9.397ValAla: 9.397 ± 0.781
1.05ValCys: 1.05 ± 0.278
5.355ValAsp: 5.355 ± 0.556
4.935ValGlu: 4.935 ± 0.562
1.995ValPhe: 1.995 ± 0.399
7.874ValGly: 7.874 ± 0.548
0.945ValHis: 0.945 ± 0.228
4.62ValIle: 4.62 ± 0.583
2.415ValLys: 2.415 ± 0.355
6.615ValLeu: 6.615 ± 0.541
1.89ValMet: 1.89 ± 0.33
2.362ValAsn: 2.362 ± 0.351
4.305ValPro: 4.305 ± 0.47
1.837ValGln: 1.837 ± 0.372
4.777ValArg: 4.777 ± 0.621
4.777ValSer: 4.777 ± 0.632
6.352ValThr: 6.352 ± 0.764
6.72ValVal: 6.72 ± 0.607
1.575ValTrp: 1.575 ± 0.426
1.68ValTyr: 1.68 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
1.995TrpAla: 1.995 ± 0.377
0.262TrpCys: 0.262 ± 0.107
1.365TrpAsp: 1.365 ± 0.267
1.417TrpGlu: 1.417 ± 0.232
0.63TrpPhe: 0.63 ± 0.215
0.892TrpGly: 0.892 ± 0.186
0.42TrpHis: 0.42 ± 0.148
0.42TrpIle: 0.42 ± 0.176
0.42TrpLys: 0.42 ± 0.194
1.365TrpLeu: 1.365 ± 0.241
0.787TrpMet: 0.787 ± 0.249
1.26TrpAsn: 1.26 ± 0.334
0.892TrpPro: 0.892 ± 0.266
0.735TrpGln: 0.735 ± 0.178
1.47TrpArg: 1.47 ± 0.29
1.732TrpSer: 1.732 ± 0.352
1.312TrpThr: 1.312 ± 0.306
2.205TrpVal: 2.205 ± 0.313
0.157TrpTrp: 0.157 ± 0.085
0.367TrpTyr: 0.367 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.1TyrAla: 2.1 ± 0.345
0.21TyrCys: 0.21 ± 0.095
2.1TyrAsp: 2.1 ± 0.357
1.417TyrGlu: 1.417 ± 0.268
0.735TyrPhe: 0.735 ± 0.176
2.467TyrGly: 2.467 ± 0.408
0.682TyrHis: 0.682 ± 0.195
0.367TyrIle: 0.367 ± 0.104
0.262TyrLys: 0.262 ± 0.108
1.995TyrLeu: 1.995 ± 0.295
0.42TyrMet: 0.42 ± 0.152
0.525TyrAsn: 0.525 ± 0.141
1.785TyrPro: 1.785 ± 0.32
0.367TyrGln: 0.367 ± 0.111
2.205TyrArg: 2.205 ± 0.39
1.627TyrSer: 1.627 ± 0.278
1.575TyrThr: 1.575 ± 0.345
1.89TyrVal: 1.89 ± 0.359
0.525TyrTrp: 0.525 ± 0.165
0.577TyrTyr: 0.577 ± 0.188
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (19050 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski