Mycobacterium phage MichelleMyBell

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Buttersvirus; Mycobacterium virus Michelle

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0LJZ5|W0LJZ5_9CAUD Lysin A OS=Mycobacterium phage MichelleMyBell OX=1445726 GN=26 PE=4 SV=1
MM1 pKa = 7.27AQAEE5 pKa = 4.32LLDD8 pKa = 3.76TDD10 pKa = 3.62MGTWCPVTLHH20 pKa = 5.79YY21 pKa = 10.61RR22 pKa = 11.84ITDD25 pKa = 3.41GDD27 pKa = 4.34EE28 pKa = 3.56ISYY31 pKa = 10.39LAVTRR36 pKa = 11.84LDD38 pKa = 3.51FVTASGRR45 pKa = 11.84VEE47 pKa = 5.38AFACDD52 pKa = 3.71EE53 pKa = 4.36NGVASTLTPLWVVDD67 pKa = 3.38GDD69 pKa = 4.15MPHH72 pKa = 7.19ADD74 pKa = 3.32ALADD78 pKa = 3.31QGYY81 pKa = 9.23EE82 pKa = 4.08VAA84 pKa = 5.55

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0LP75|W0LP75_9CAUD Uncharacterized protein OS=Mycobacterium phage MichelleMyBell OX=1445726 GN=70 PE=4 SV=1
MM1 pKa = 7.63SMNFHH6 pKa = 7.45LSRR9 pKa = 11.84AEE11 pKa = 3.81QARR14 pKa = 11.84LRR16 pKa = 11.84EE17 pKa = 4.11KK18 pKa = 10.37LWYY21 pKa = 10.06VPEE24 pKa = 4.29LAGDD28 pKa = 4.2LAVTIARR35 pKa = 11.84QARR38 pKa = 11.84IQKK41 pKa = 9.75PNLGKK46 pKa = 9.64PRR48 pKa = 11.84RR49 pKa = 11.84QRR51 pKa = 11.84PEE53 pKa = 3.52PCVPFHH59 pKa = 6.79LGASEE64 pKa = 3.85AAEE67 pKa = 3.96EE68 pKa = 4.1LHH70 pKa = 6.8RR71 pKa = 11.84CLTGWVRR78 pKa = 11.84FVCDD82 pKa = 3.82ARR84 pKa = 11.84QVDD87 pKa = 4.06YY88 pKa = 11.31TGTDD92 pKa = 3.4DD93 pKa = 4.69LASLARR99 pKa = 11.84WLHH102 pKa = 5.76RR103 pKa = 11.84NVVTLALIEE112 pKa = 4.43GSEE115 pKa = 3.97RR116 pKa = 11.84AYY118 pKa = 11.25VDD120 pKa = 2.8IAHH123 pKa = 7.57RR124 pKa = 11.84IDD126 pKa = 3.1EE127 pKa = 4.41CRR129 pKa = 11.84RR130 pKa = 11.84QIDD133 pKa = 4.55LPPEE137 pKa = 4.1DD138 pKa = 5.06EE139 pKa = 4.18IVIDD143 pKa = 4.32RR144 pKa = 11.84ARR146 pKa = 11.84LEE148 pKa = 3.86QANRR152 pKa = 11.84QIVTAGQAEE161 pKa = 4.51KK162 pKa = 10.32IARR165 pKa = 11.84KK166 pKa = 10.01LGDD169 pKa = 3.51LGRR172 pKa = 11.84RR173 pKa = 11.84LTTQRR178 pKa = 11.84VHH180 pKa = 5.56SLNRR184 pKa = 11.84RR185 pKa = 11.84GHH187 pKa = 6.09LRR189 pKa = 11.84PVDD192 pKa = 3.76TDD194 pKa = 3.76PEE196 pKa = 4.57TGTKK200 pKa = 10.03FYY202 pKa = 11.04RR203 pKa = 11.84LGDD206 pKa = 3.34ILQAHH211 pKa = 6.54MKK213 pKa = 9.52CAQRR217 pKa = 11.84QRR219 pKa = 11.84RR220 pKa = 11.84SS221 pKa = 3.04

Molecular weight: 25.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13684

36

1033

195.5

21.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.116 ± 0.667

0.979 ± 0.143

6.584 ± 0.242

6.007 ± 0.333

2.821 ± 0.173

9.12 ± 0.626

1.966 ± 0.148

4.721 ± 0.167

2.865 ± 0.208

7.622 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.958 ± 0.125

3.047 ± 0.167

6.08 ± 0.201

3.639 ± 0.201

7.103 ± 0.402

5.269 ± 0.248

6.548 ± 0.241

7.235 ± 0.322

1.922 ± 0.144

2.397 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski