Arabis alpina (Alpine rock-cress)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087HHS5|A0A087HHS5_ARAAL Lipoxygenase OS=Arabis alpina OX=50452 GN=AALP_AA2G158600 PE=3 SV=1
MM1 pKa = 6.49NTVVFLMVYY10 pKa = 9.21QKK12 pKa = 11.16NVFNDD17 pKa = 3.35VYY19 pKa = 11.05GGVPEE24 pKa = 5.42DD25 pKa = 3.83VLEE28 pKa = 4.72DD29 pKa = 4.12GYY31 pKa = 11.82GGVFDD36 pKa = 4.88GVLEE40 pKa = 4.2GVYY43 pKa = 10.66GSVPNGVNGVPDD55 pKa = 4.06GVLVGVPVSVPGGGDD70 pKa = 3.03GGIPGNVHH78 pKa = 6.92DD79 pKa = 5.0SVPKK83 pKa = 9.4VVYY86 pKa = 10.22GGVPTAVLEE95 pKa = 4.23NVDD98 pKa = 3.41GGVPVGLPEE107 pKa = 4.44GRR109 pKa = 11.84DD110 pKa = 3.27GGIPDD115 pKa = 3.94NVHH118 pKa = 6.93DD119 pKa = 4.71GVPKK123 pKa = 10.71VVDD126 pKa = 3.97DD127 pKa = 4.39GVPAGVDD134 pKa = 3.14GGVPAGVDD142 pKa = 3.17GGVPADD148 pKa = 3.55VSEE151 pKa = 4.3VLDD154 pKa = 3.98GGVPDD159 pKa = 5.89GIPEE163 pKa = 4.29GVDD166 pKa = 2.79NGVQGEE172 pKa = 4.3DD173 pKa = 3.14GGIPGTVWKK182 pKa = 10.32VKK184 pKa = 8.42TVVYY188 pKa = 8.13QTVCQIVYY196 pKa = 10.27RR197 pKa = 11.84IMYY200 pKa = 7.24TAVYY204 pKa = 8.71WKK206 pKa = 10.66LL207 pKa = 3.25

Molecular weight: 20.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087FY95|A0A087FY95_ARAAL Uncharacterized protein OS=Arabis alpina OX=50452 GN=AALP_AAs54367U000100 PE=3 SV=1
DDD2 pKa = 4.01AGGVLVRR9 pKa = 11.84AVPAQRR15 pKa = 11.84RR16 pKa = 11.84AVVPAAGARR25 pKa = 11.84AALLAAGAPGPQRR38 pKa = 11.84QGLRR42 pKa = 11.84LQPARR47 pKa = 11.84RR48 pKa = 11.84HHH50 pKa = 4.62RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84PRR55 pKa = 11.84AQRR58 pKa = 11.84HHH60 pKa = 5.03PPVQGARR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84PGRR72 pKa = 11.84VRR74 pKa = 11.84VPQGHHH80 pKa = 6.1PVSV

Molecular weight: 9.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21573

1672

23245

9104347

8

5083

391.7

43.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.572 ± 0.016

1.731 ± 0.008

5.544 ± 0.011

6.923 ± 0.02

4.157 ± 0.011

6.497 ± 0.016

2.195 ± 0.007

5.184 ± 0.012

6.343 ± 0.018

9.434 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.007

4.246 ± 0.011

4.919 ± 0.014

3.405 ± 0.011

5.513 ± 0.013

8.991 ± 0.021

5.181 ± 0.011

6.79 ± 0.014

1.217 ± 0.005

2.706 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski