Gordonia phage GEazy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bowservirus; unclassified Bowservirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KRZ8|A0A345KRZ8_9CAUD RHH_1 domain-containing protein OS=Gordonia phage GEazy OX=2250393 GN=38 PE=4 SV=1
MM1 pKa = 7.11FHH3 pKa = 7.89DD4 pKa = 3.75ILAVIVCVAVAGWLLAGPSNDD25 pKa = 3.76NGPPSALEE33 pKa = 4.65DD34 pKa = 4.11GPPGNGLDD42 pKa = 3.71AYY44 pKa = 9.65PLGGCGSLPP53 pKa = 3.41

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KS30|A0A345KS30_9CAUD HNH endonuclease OS=Gordonia phage GEazy OX=2250393 GN=73 PE=4 SV=1
MM1 pKa = 8.02PEE3 pKa = 3.53TPAPHH8 pKa = 6.91RR9 pKa = 11.84CRR11 pKa = 11.84GFRR14 pKa = 11.84RR15 pKa = 11.84WCGGYY20 pKa = 10.75RR21 pKa = 11.84MTQTAAVLAPEE32 pKa = 5.16GSDD35 pKa = 3.46SLLTADD41 pKa = 5.27EE42 pKa = 4.47AATLCGVSAKK52 pKa = 7.58TVRR55 pKa = 11.84AWANRR60 pKa = 11.84GYY62 pKa = 8.92CTRR65 pKa = 11.84NGARR69 pKa = 11.84QKK71 pKa = 10.98LDD73 pKa = 3.16VVGRR77 pKa = 11.84DD78 pKa = 3.12EE79 pKa = 5.09RR80 pKa = 11.84GRR82 pKa = 11.84NLYY85 pKa = 10.13RR86 pKa = 11.84AIDD89 pKa = 3.72VAKK92 pKa = 10.71AEE94 pKa = 4.43YY95 pKa = 8.43ATSRR99 pKa = 11.84RR100 pKa = 11.84SRR102 pKa = 11.84RR103 pKa = 3.4

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15071

39

1707

206.5

22.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.673 ± 0.545

0.922 ± 0.176

6.536 ± 0.325

5.567 ± 0.38

2.707 ± 0.222

8.533 ± 0.47

2.09 ± 0.192

4.412 ± 0.225

2.873 ± 0.184

8.188 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.12

2.661 ± 0.195

6.078 ± 0.394

3.331 ± 0.166

7.65 ± 0.377

5.773 ± 0.188

6.642 ± 0.254

7.226 ± 0.306

2.064 ± 0.148

1.878 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski