Cynoglossus semilaevis (Tongue sole)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32408 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P8VKF8|A0A3P8VKF8_CYNSE TraB domain containing OS=Cynoglossus semilaevis OX=244447 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 4.36STSQAYY8 pKa = 9.98SGAEE12 pKa = 4.11DD13 pKa = 3.99NSTDD17 pKa = 3.58PQEE20 pKa = 4.47TLTEE24 pKa = 4.17TSNLTFMDD32 pKa = 4.23APTFDD37 pKa = 5.05GPAEE41 pKa = 4.14NCSLHH46 pKa = 6.76GVTTVCLKK54 pKa = 10.73EE55 pKa = 4.07FVLIDD60 pKa = 4.7DD61 pKa = 5.57DD62 pKa = 6.02DD63 pKa = 6.81DD64 pKa = 7.3GDD66 pKa = 3.45MSLRR70 pKa = 11.84EE71 pKa = 4.03KK72 pKa = 10.25TVTDD76 pKa = 3.91LSIMNGRR83 pKa = 11.84AAEE86 pKa = 4.13LVCGRR91 pKa = 11.84VLAFSSSSEE100 pKa = 3.97SDD102 pKa = 3.17CGEE105 pKa = 4.26EE106 pKa = 3.84EE107 pKa = 4.34TVAPEE112 pKa = 4.1TPTVEE117 pKa = 5.52DD118 pKa = 3.74VVTPQGSGRR127 pKa = 11.84CCSCTVSS134 pKa = 3.22

Molecular weight: 14.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P8WYF4|A0A3P8WYF4_CYNSE Alpha-1-antitrypsin homolog OS=Cynoglossus semilaevis OX=244447 PE=3 SV=1
MM1 pKa = 7.41LQQPSRR7 pKa = 11.84PVKK10 pKa = 9.83QAPQIPQQPSRR21 pKa = 11.84PVMQPSRR28 pKa = 11.84PVMQPSRR35 pKa = 11.84PVMQPSRR42 pKa = 11.84PVKK45 pKa = 10.48QPFQQPTRR53 pKa = 11.84RR54 pKa = 11.84PVKK57 pKa = 10.36QPSQQPTRR65 pKa = 11.84PVKK68 pKa = 10.52QPSQQPTRR76 pKa = 11.84PVKK79 pKa = 10.02QPNWPVKK86 pKa = 9.83QAPQQPTRR94 pKa = 11.84PVKK97 pKa = 10.52QPSQQPTRR105 pKa = 11.84PVKK108 pKa = 10.02QPNWPVKK115 pKa = 9.83QAPQQPSRR123 pKa = 11.84PVKK126 pKa = 9.91QPSRR130 pKa = 11.84PVKK133 pKa = 10.42QPSQQPTRR141 pKa = 11.84PVKK144 pKa = 10.07QVPQQPSRR152 pKa = 11.84PVMQPSRR159 pKa = 11.84PVKK162 pKa = 9.68QPNWPVKK169 pKa = 9.94QPSRR173 pKa = 11.84PVKK176 pKa = 9.92QAPQQPTPPVKK187 pKa = 10.35QPSRR191 pKa = 11.84PVKK194 pKa = 10.15QPPPQPSRR202 pKa = 11.84PVKK205 pKa = 9.91QPSRR209 pKa = 11.84RR210 pKa = 11.84PPRR213 pKa = 11.84PVKK216 pKa = 9.94QAPQIPLISGII227 pKa = 3.97

Molecular weight: 25.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21333

11075

32408

18528367

19

6092

571.7

63.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.263 ± 0.01

2.295 ± 0.012

5.198 ± 0.01

6.767 ± 0.018

3.682 ± 0.01

6.236 ± 0.017

2.698 ± 0.007

4.372 ± 0.012

5.638 ± 0.015

9.544 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.005

3.945 ± 0.008

5.619 ± 0.017

4.78 ± 0.014

5.578 ± 0.01

8.758 ± 0.017

5.779 ± 0.01

6.477 ± 0.011

1.153 ± 0.004

2.744 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski