Capsella rubella

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28039 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R0GIK7|R0GIK7_9BRAS Uncharacterized protein OS=Capsella rubella OX=81985 GN=CARUB_v10020743mg PE=4 SV=1
MM1 pKa = 7.56AGDD4 pKa = 4.11EE5 pKa = 4.43PKK7 pKa = 10.81SSAVKK12 pKa = 10.51DD13 pKa = 4.12DD14 pKa = 4.3ADD16 pKa = 3.83EE17 pKa = 4.93DD18 pKa = 4.95PYY20 pKa = 11.44NHH22 pKa = 7.5PYY24 pKa = 10.98DD25 pKa = 3.87GFGWFQNVMDD35 pKa = 5.32DD36 pKa = 4.14PNPNLMDD43 pKa = 3.41MAFTGNTSPSMCPPLLPLDD62 pKa = 3.8VDD64 pKa = 3.76QTQLFHH70 pKa = 7.15EE71 pKa = 5.22LDD73 pKa = 3.9LSISNYY79 pKa = 8.24DD80 pKa = 3.54TNDD83 pKa = 3.41DD84 pKa = 3.84FDD86 pKa = 4.03VLSFFDD92 pKa = 5.41DD93 pKa = 5.4GITTSLDD100 pKa = 3.12MLEE103 pKa = 4.76PLNDD107 pKa = 3.94GPNEE111 pKa = 4.01NVNNDD116 pKa = 3.25SQGEE120 pKa = 4.02ITYY123 pKa = 10.39DD124 pKa = 3.36YY125 pKa = 11.87VNDD128 pKa = 3.94HH129 pKa = 6.56NNNNVGEE136 pKa = 4.39TTRR139 pKa = 11.84LEE141 pKa = 3.83VSTRR145 pKa = 11.84VFGDD149 pKa = 3.41PNGPNYY155 pKa = 10.3EE156 pKa = 4.06IGGTSTTPVASDD168 pKa = 3.42KK169 pKa = 11.5DD170 pKa = 4.14FACDD174 pKa = 3.16CCTMLRR180 pKa = 11.84EE181 pKa = 4.09LVHH184 pKa = 5.8MKK186 pKa = 9.98EE187 pKa = 4.17GEE189 pKa = 3.99IMTTLVIYY197 pKa = 10.31GGIGFICHH205 pKa = 6.81AFLHH209 pKa = 5.92TRR211 pKa = 11.84LLPSDD216 pKa = 3.62STEE219 pKa = 4.02PQPQNQPEE227 pKa = 4.15PQKK230 pKa = 10.59IHH232 pKa = 7.41LMDD235 pKa = 3.73LTMEE239 pKa = 4.16EE240 pKa = 4.07VKK242 pKa = 10.79KK243 pKa = 10.8FIEE246 pKa = 5.11DD247 pKa = 3.61YY248 pKa = 10.65CSQRR252 pKa = 11.84LASGLSLVQDD262 pKa = 3.98TNAAFYY268 pKa = 10.73EE269 pKa = 4.25AMSVNSIFNQSPPMLAPSTFTEE291 pKa = 4.2ALDD294 pKa = 3.73VSLVPLNVGLATEE307 pKa = 4.55EE308 pKa = 4.1PTAA311 pKa = 4.21

Molecular weight: 34.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R0IG64|R0IG64_9BRAS HVA22-like protein OS=Capsella rubella OX=81985 GN=CARUB_v10021029mg PE=3 SV=1
FF1 pKa = 6.56THH3 pKa = 7.1LHH5 pKa = 4.71TTHH8 pKa = 7.17TFTNLRR14 pKa = 11.84LLLTTRR20 pKa = 11.84LLNRR24 pKa = 11.84LLKK27 pKa = 10.61RR28 pKa = 11.84SLTTIQKK35 pKa = 7.75KK36 pKa = 7.34TKK38 pKa = 9.41NRR40 pKa = 11.84LVII43 pKa = 3.97

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26721

1318

28039

11560300

8

5389

412.3

46.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.323 ± 0.013

1.879 ± 0.008

5.408 ± 0.011

6.686 ± 0.019

4.275 ± 0.01

6.352 ± 0.012

2.291 ± 0.005

5.272 ± 0.011

6.373 ± 0.014

9.578 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.006

4.387 ± 0.01

4.802 ± 0.013

3.482 ± 0.01

5.404 ± 0.011

9.159 ± 0.018

5.106 ± 0.009

6.689 ± 0.01

1.25 ± 0.005

2.835 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski