Flavihumibacter petaseus NBRC 106054

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Flavihumibacter; Flavihumibacter petaseus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4759 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E9N733|A0A0E9N733_9BACT Putative two-component response regulator OS=Flavihumibacter petaseus NBRC 106054 OX=1220578 GN=FPE01S_05_02010 PE=4 SV=1
LL1 pKa = 7.08PATVTIDD8 pKa = 3.33QPAVLTASAAGDD20 pKa = 4.27DD21 pKa = 4.13VTCYY25 pKa = 10.41NAANGTITVSSPTGGSGEE43 pKa = 4.06FEE45 pKa = 3.88FRR47 pKa = 11.84INGGTWQEE55 pKa = 3.77GLVFSNLAPDD65 pKa = 4.4TYY67 pKa = 10.9TIDD70 pKa = 4.4IRR72 pKa = 11.84DD73 pKa = 4.02KK74 pKa = 11.32NNPLCIVTLPEE85 pKa = 4.36TVTIDD90 pKa = 3.43QPAVLSASAAGDD102 pKa = 3.59DD103 pKa = 3.97VTCFNAANGKK113 pKa = 8.28ITVSSPTGGSGEE125 pKa = 4.1YY126 pKa = 10.05EE127 pKa = 3.76FRR129 pKa = 11.84INGGTWQEE137 pKa = 3.77GLVFSNLAPDD147 pKa = 4.4TYY149 pKa = 10.9TIDD152 pKa = 3.71IRR154 pKa = 11.84DD155 pKa = 4.11KK156 pKa = 9.98NTPACIVTLPGTVTIDD172 pKa = 3.31QPAVLSASAAGDD184 pKa = 4.18DD185 pKa = 4.02VTCYY189 pKa = 10.42NAANGKK195 pKa = 8.28ITVSSPTGGSGEE207 pKa = 4.1YY208 pKa = 10.05EE209 pKa = 3.76FRR211 pKa = 11.84INGGTT216 pKa = 3.2

Molecular weight: 22.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E9N0S3|A0A0E9N0S3_9BACT Uncharacterized protein OS=Flavihumibacter petaseus NBRC 106054 OX=1220578 GN=FPE01S_02_07180 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84NAIAFACQIPRR13 pKa = 11.84EE14 pKa = 4.35NILPATRR21 pKa = 11.84LEE23 pKa = 3.94DD24 pKa = 4.75LIPVRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84QSIVKK36 pKa = 9.96KK37 pKa = 10.49INTLLGLRR45 pKa = 11.84LINLSITDD53 pKa = 4.04RR54 pKa = 11.84LALTWLITTVCSLTLFFISWKK75 pKa = 10.31LAVSCLLINFSAIWLSVKK93 pKa = 8.44MAKK96 pKa = 9.98LFQMEE101 pKa = 4.5TVGALARR108 pKa = 11.84HH109 pKa = 5.86FAWHH113 pKa = 6.7NYY115 pKa = 5.27RR116 pKa = 11.84TARR119 pKa = 11.84RR120 pKa = 11.84DD121 pKa = 3.31PASYY125 pKa = 10.58NPRR128 pKa = 11.84EE129 pKa = 4.11VTPFLINAFTSHH141 pKa = 6.94FDD143 pKa = 3.43IDD145 pKa = 4.51PKK147 pKa = 10.9TLTADD152 pKa = 3.18TRR154 pKa = 11.84LL155 pKa = 3.47

Molecular weight: 17.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4759

0

4759

1741998

50

2989

366.0

40.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.272 ± 0.035

0.832 ± 0.011

5.34 ± 0.023

5.417 ± 0.039

4.742 ± 0.025

7.444 ± 0.037

1.928 ± 0.017

6.352 ± 0.025

5.722 ± 0.034

9.605 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.018

4.917 ± 0.035

4.381 ± 0.027

3.994 ± 0.022

4.836 ± 0.029

6.192 ± 0.031

5.834 ± 0.052

6.481 ± 0.022

1.468 ± 0.014

3.901 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski