Hubei sobemo-like virus 33

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEF7|A0A1L3KEF7_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 33 OX=1923220 PE=4 SV=1
MM1 pKa = 7.51PGKK4 pKa = 9.42MDD6 pKa = 5.8LIHH9 pKa = 8.25IEE11 pKa = 4.42EE12 pKa = 4.27ILLNRR17 pKa = 11.84WKK19 pKa = 10.45AARR22 pKa = 11.84WRR24 pKa = 11.84IPDD27 pKa = 3.83DD28 pKa = 3.57FLEE31 pKa = 4.52RR32 pKa = 11.84THH34 pKa = 6.67FEE36 pKa = 4.12RR37 pKa = 11.84VVLEE41 pKa = 4.04IDD43 pKa = 3.3WTSSPGYY50 pKa = 9.28PWLLQHH56 pKa = 5.92TTNSAFFEE64 pKa = 4.52VKK66 pKa = 10.16DD67 pKa = 4.06GKK69 pKa = 9.78PSKK72 pKa = 10.5AALDD76 pKa = 3.92RR77 pKa = 11.84VWTIVQQRR85 pKa = 11.84LQSRR89 pKa = 11.84EE90 pKa = 3.93CDD92 pKa = 4.03PIRR95 pKa = 11.84LFIKK99 pKa = 10.28PEE101 pKa = 3.49PHH103 pKa = 6.05KK104 pKa = 10.66QKK106 pKa = 11.03KK107 pKa = 9.65LDD109 pKa = 3.53SKK111 pKa = 11.1AYY113 pKa = 9.65RR114 pKa = 11.84LISSVSVIDD123 pKa = 4.07QIIDD127 pKa = 3.25ALLFGEE133 pKa = 4.6MNQRR137 pKa = 11.84MIEE140 pKa = 4.08RR141 pKa = 11.84YY142 pKa = 9.69LDD144 pKa = 3.47VPGKK148 pKa = 10.38VGWSPYY154 pKa = 8.79VGGWKK159 pKa = 9.98IMPAYY164 pKa = 10.51GNVSLDD170 pKa = 3.05KK171 pKa = 11.14SAWDD175 pKa = 3.32WSVNAWIIQCILNMRR190 pKa = 11.84MQLCDD195 pKa = 3.49NVSEE199 pKa = 4.23SWIDD203 pKa = 3.51LACWRR208 pKa = 11.84YY209 pKa = 8.74NALYY213 pKa = 10.16NSPLFVTSGGLLLRR227 pKa = 11.84QLQPGVVKK235 pKa = 10.42SGCYY239 pKa = 7.84NTIADD244 pKa = 3.88NSIAQDD250 pKa = 3.77LLHH253 pKa = 6.72VRR255 pKa = 11.84VCVEE259 pKa = 4.29LDD261 pKa = 3.5IEE263 pKa = 4.88CGDD266 pKa = 4.21LMSMGDD272 pKa = 3.88DD273 pKa = 3.32TTQRR277 pKa = 11.84WFSEE281 pKa = 4.29FPDD284 pKa = 3.64YY285 pKa = 11.33VEE287 pKa = 5.78RR288 pKa = 11.84LSQYY292 pKa = 9.62CHH294 pKa = 5.8VKK296 pKa = 10.5GSVNGTEE303 pKa = 4.31FAGHH307 pKa = 7.27RR308 pKa = 11.84FTSCSVEE315 pKa = 3.63PLYY318 pKa = 10.72KK319 pKa = 10.42GKK321 pKa = 9.56HH322 pKa = 5.99AYY324 pKa = 10.71NIMNMNPKK332 pKa = 10.14YY333 pKa = 10.68GEE335 pKa = 3.93EE336 pKa = 4.04TAASYY341 pKa = 11.51ALLYY345 pKa = 9.92HH346 pKa = 6.8RR347 pKa = 11.84SVHH350 pKa = 5.74SNYY353 pKa = 9.07IRR355 pKa = 11.84ALLEE359 pKa = 3.88RR360 pKa = 11.84MGYY363 pKa = 7.08TLPSRR368 pKa = 11.84QTLDD372 pKa = 3.22VIYY375 pKa = 10.62DD376 pKa = 3.83GEE378 pKa = 4.36VV379 pKa = 2.61

Molecular weight: 43.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEF7|A0A1L3KEF7_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 33 OX=1923220 PE=4 SV=1
MM1 pKa = 7.36FPLIAMIKK9 pKa = 8.71VFCCVLTAGWLRR21 pKa = 11.84YY22 pKa = 9.11IYY24 pKa = 10.35LRR26 pKa = 11.84VEE28 pKa = 4.57MIPGLLAAWLIRR40 pKa = 11.84MEE42 pKa = 4.75EE43 pKa = 3.8KK44 pKa = 10.48WMDD47 pKa = 3.95ALDD50 pKa = 3.49AWVCRR55 pKa = 11.84IALGPSEE62 pKa = 4.99KK63 pKa = 9.88PAPVPLTFWEE73 pKa = 4.31EE74 pKa = 3.75MVYY77 pKa = 9.64TLSPYY82 pKa = 9.98FDD84 pKa = 3.26VAIRR88 pKa = 11.84RR89 pKa = 11.84LVFIGYY95 pKa = 9.23SLTAIFVVVLAFMAVKK111 pKa = 10.23KK112 pKa = 9.8PLRR115 pKa = 11.84RR116 pKa = 11.84TVLRR120 pKa = 11.84LRR122 pKa = 11.84GITFEE127 pKa = 4.02SMQPGSEE134 pKa = 4.14FVEE137 pKa = 4.81GAVPDD142 pKa = 4.37FQVGIYY148 pKa = 10.27DD149 pKa = 3.75AGTFVDD155 pKa = 3.75TFIGYY160 pKa = 9.06GIRR163 pKa = 11.84FGSLLVLPAHH173 pKa = 6.43VIKK176 pKa = 10.51HH177 pKa = 5.04VKK179 pKa = 9.38QLVIEE184 pKa = 4.47GRR186 pKa = 11.84TGRR189 pKa = 11.84LGLNTSYY196 pKa = 10.72IPSKK200 pKa = 10.87VSTDD204 pKa = 3.28LVYY207 pKa = 10.62IPMTEE212 pKa = 4.19AQWSRR217 pKa = 11.84VGTSTAKK224 pKa = 10.27VPKK227 pKa = 9.99KK228 pKa = 10.28LVNSLVSCTGRR239 pKa = 11.84KK240 pKa = 7.59GTSTGLLTQTDD251 pKa = 3.86VLGIMKK257 pKa = 10.33YY258 pKa = 10.14SGSTISGMSGAAYY271 pKa = 10.43YY272 pKa = 10.5SGQTVYY278 pKa = 11.36GMHH281 pKa = 6.62TGVAGDD287 pKa = 3.67YY288 pKa = 10.6NIGVTATLIMAEE300 pKa = 4.25TRR302 pKa = 11.84RR303 pKa = 11.84LVLGEE308 pKa = 4.44SPTLDD313 pKa = 2.98EE314 pKa = 4.36MKK316 pKa = 10.74GLNRR320 pKa = 11.84ASAAKK325 pKa = 9.93VRR327 pKa = 11.84SGWDD331 pKa = 3.13TTHH334 pKa = 7.21LMKK337 pKa = 10.73QVDD340 pKa = 3.71SMYY343 pKa = 10.94ADD345 pKa = 4.33EE346 pKa = 5.36IGWAADD352 pKa = 3.19EE353 pKa = 4.16EE354 pKa = 4.35MDD356 pKa = 4.78YY357 pKa = 11.36GVQLTFDD364 pKa = 4.12GEE366 pKa = 4.18ASEE369 pKa = 4.19EE370 pKa = 4.26AIEE373 pKa = 3.91KK374 pKa = 10.09WMTFFTAMPSMQRR387 pKa = 11.84EE388 pKa = 4.03SAINLLQSYY397 pKa = 8.8TNASRR402 pKa = 11.84AAKK405 pKa = 9.48GQSDD409 pKa = 4.67EE410 pKa = 4.43PTPITLPKK418 pKa = 10.37DD419 pKa = 3.87FVSMRR424 pKa = 11.84LDD426 pKa = 4.08AIEE429 pKa = 4.45KK430 pKa = 10.34RR431 pKa = 11.84LDD433 pKa = 3.74KK434 pKa = 10.23ITALEE439 pKa = 3.97QRR441 pKa = 11.84VKK443 pKa = 10.03TLEE446 pKa = 3.93EE447 pKa = 4.37KK448 pKa = 10.39IVAGVRR454 pKa = 11.84KK455 pKa = 9.34PGIAIPNHH463 pKa = 5.36SPKK466 pKa = 10.22PFPCGYY472 pKa = 8.37EE473 pKa = 3.68KK474 pKa = 10.64CYY476 pKa = 10.66KK477 pKa = 10.56AFNKK481 pKa = 10.13PEE483 pKa = 3.62ARR485 pKa = 11.84MAHH488 pKa = 5.01QVAVGHH494 pKa = 5.51VVGEE498 pKa = 4.41SAFGADD504 pKa = 3.92EE505 pKa = 4.39KK506 pKa = 10.26PTVGTAPSPVFRR518 pKa = 11.84QRR520 pKa = 11.84PLQKK524 pKa = 10.01KK525 pKa = 9.55KK526 pKa = 10.4PSTSSRR532 pKa = 11.84NSSSSQEE539 pKa = 3.7RR540 pKa = 11.84GKK542 pKa = 10.03PSTSQQQNQSDD553 pKa = 4.13TLVSLMRR560 pKa = 11.84TIGDD564 pKa = 3.63LQKK567 pKa = 11.11SLEE570 pKa = 4.19ATVGLLSATTQNSTVV585 pKa = 3.11

Molecular weight: 64.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

964

379

585

482.0

54.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.054 ± 1.071

1.556 ± 0.493

4.876 ± 0.878

5.602 ± 0.037

3.32 ± 0.251

6.95 ± 0.69

1.763 ± 0.527

5.705 ± 0.378

5.394 ± 0.389

9.025 ± 0.445

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.631 ± 0.28

2.801 ± 0.856

4.979 ± 0.298

3.838 ± 0.072

5.602 ± 0.281

7.884 ± 0.14

6.224 ± 1.525

7.676 ± 0.492

2.282 ± 0.692

3.838 ± 0.549
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski