Amino acid dipepetide frequency for Rhizobium sp. Leaf306

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.753AlaAla: 14.753 ± 0.125	0.924AlaCys: 0.924 ± 0.025	6.778AlaAsp: 6.778 ± 0.072	6.94AlaGlu: 6.94 ± 0.078	4.54AlaPhe: 4.54 ± 0.062	9.66AlaGly: 9.66 ± 0.094	1.972AlaHis: 1.972 ± 0.036	6.883AlaIle: 6.883 ± 0.067	4.558AlaLys: 4.558 ± 0.067	11.965AlaLeu: 11.965 ± 0.127	3.509AlaMet: 3.509 ± 0.052	2.942AlaAsn: 2.942 ± 0.046	4.645AlaPro: 4.645 ± 0.07	3.563AlaGln: 3.563 ± 0.059	6.939AlaArg: 6.939 ± 0.083	6.982AlaSer: 6.982 ± 0.079	5.887AlaThr: 5.887 ± 0.073	8.395AlaVal: 8.395 ± 0.09	1.236AlaTrp: 1.236 ± 0.035	2.544AlaTyr: 2.544 ± 0.046	0.001AlaXaa: 0.001 ± 0.001
Cys	0.785CysAla: 0.785 ± 0.022	0.092CysCys: 0.092 ± 0.009	0.471CysAsp: 0.471 ± 0.018	0.421CysGlu: 0.421 ± 0.017	0.319CysPhe: 0.319 ± 0.016	0.852CysGly: 0.852 ± 0.028	0.208CysHis: 0.208 ± 0.013	0.381CysIle: 0.381 ± 0.016	0.185CysLys: 0.185 ± 0.012	0.782CysLeu: 0.782 ± 0.025	0.156CysMet: 0.156 ± 0.01	0.213CysAsn: 0.213 ± 0.012	0.337CysPro: 0.337 ± 0.017	0.205CysGln: 0.205 ± 0.012	0.521CysArg: 0.521 ± 0.023	0.437CysSer: 0.437 ± 0.019	0.353CysThr: 0.353 ± 0.015	0.561CysVal: 0.561 ± 0.02	0.091CysTrp: 0.091 ± 0.008	0.167CysTyr: 0.167 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.5AspAla: 6.5 ± 0.078	0.445AspCys: 0.445 ± 0.017	3.379AspAsp: 3.379 ± 0.056	3.565AspGlu: 3.565 ± 0.056	2.31AspPhe: 2.31 ± 0.042	5.289AspGly: 5.289 ± 0.075	1.281AspHis: 1.281 ± 0.034	3.571AspIle: 3.571 ± 0.049	2.036AspLys: 2.036 ± 0.04	6.139AspLeu: 6.139 ± 0.069	1.582AspMet: 1.582 ± 0.033	1.537AspAsn: 1.537 ± 0.036	3.194AspPro: 3.194 ± 0.052	1.872AspGln: 1.872 ± 0.041	4.192AspArg: 4.192 ± 0.052	2.18AspSer: 2.18 ± 0.043	2.696AspThr: 2.696 ± 0.043	4.356AspVal: 4.356 ± 0.065	0.916AspTrp: 0.916 ± 0.024	1.545AspTyr: 1.545 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.935GluAla: 6.935 ± 0.083	0.338GluCys: 0.338 ± 0.018	2.954GluAsp: 2.954 ± 0.052	3.343GluGlu: 3.343 ± 0.064	1.936GluPhe: 1.936 ± 0.039	4.142GluGly: 4.142 ± 0.058	1.104GluHis: 1.104 ± 0.03	3.857GluIle: 3.857 ± 0.052	2.879GluLys: 2.879 ± 0.05	5.22GluLeu: 5.22 ± 0.059	1.585GluMet: 1.585 ± 0.035	1.898GluAsn: 1.898 ± 0.034	2.62GluPro: 2.62 ± 0.046	2.126GluGln: 2.126 ± 0.041	4.299GluArg: 4.299 ± 0.064	2.332GluSer: 2.332 ± 0.043	3.92GluThr: 3.92 ± 0.068	3.731GluVal: 3.731 ± 0.051	0.687GluTrp: 0.687 ± 0.022	1.056GluTyr: 1.056 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.499PheAla: 4.499 ± 0.054	0.37PheCys: 0.37 ± 0.015	2.822PheAsp: 2.822 ± 0.046	2.316PheGlu: 2.316 ± 0.042	1.576PhePhe: 1.576 ± 0.044	3.902PheGly: 3.902 ± 0.06	0.785PheHis: 0.785 ± 0.025	1.943PheIle: 1.943 ± 0.05	1.222PheLys: 1.222 ± 0.033	3.619PheLeu: 3.619 ± 0.055	0.903PheMet: 0.903 ± 0.026	1.138PheAsn: 1.138 ± 0.028	1.676PhePro: 1.676 ± 0.032	1.116PheGln: 1.116 ± 0.027	2.237PheArg: 2.237 ± 0.047	2.715PheSer: 2.715 ± 0.045	2.021PheThr: 2.021 ± 0.036	3.015PheVal: 3.015 ± 0.046	0.575PheTrp: 0.575 ± 0.021	0.976PheTyr: 0.976 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	8.181GlyAla: 8.181 ± 0.092	0.761GlyCys: 0.761 ± 0.024	4.458GlyAsp: 4.458 ± 0.055	4.76GlyGlu: 4.76 ± 0.059	3.752GlyPhe: 3.752 ± 0.052	6.911GlyGly: 6.911 ± 0.094	1.804GlyHis: 1.804 ± 0.039	5.157GlyIle: 5.157 ± 0.053	3.703GlyLys: 3.703 ± 0.053	8.605GlyLeu: 8.605 ± 0.093	2.474GlyMet: 2.474 ± 0.045	2.413GlyAsn: 2.413 ± 0.048	3.129GlyPro: 3.129 ± 0.048	2.78GlyGln: 2.78 ± 0.049	5.552GlyArg: 5.552 ± 0.066	5.079GlySer: 5.079 ± 0.055	4.592GlyThr: 4.592 ± 0.058	5.784GlyVal: 5.784 ± 0.062	1.224GlyTrp: 1.224 ± 0.029	2.367GlyTyr: 2.367 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.102HisAla: 2.102 ± 0.042	0.206HisCys: 0.206 ± 0.013	1.245HisAsp: 1.245 ± 0.03	1.059HisGlu: 1.059 ± 0.024	0.881HisPhe: 0.881 ± 0.026	1.818HisGly: 1.818 ± 0.039	0.531HisHis: 0.531 ± 0.025	0.968HisIle: 0.968 ± 0.024	0.534HisLys: 0.534 ± 0.021	2.001HisLeu: 2.001 ± 0.041	0.508HisMet: 0.508 ± 0.019	0.483HisAsn: 0.483 ± 0.02	1.228HisPro: 1.228 ± 0.027	0.56HisGln: 0.56 ± 0.02	1.275HisArg: 1.275 ± 0.035	1.009HisSer: 1.009 ± 0.026	0.802HisThr: 0.802 ± 0.024	1.5HisVal: 1.5 ± 0.031	0.272HisTrp: 0.272 ± 0.014	0.499HisTyr: 0.499 ± 0.019	0.001HisXaa: 0.001 ± 0.001
Ile	7.824IleAla: 7.824 ± 0.088	0.545IleCys: 0.545 ± 0.019	3.951IleAsp: 3.951 ± 0.061	3.807IleGlu: 3.807 ± 0.054	2.06IlePhe: 2.06 ± 0.037	5.464IleGly: 5.464 ± 0.069	0.936IleHis: 0.936 ± 0.026	2.78IleIle: 2.78 ± 0.051	1.714IleLys: 1.714 ± 0.036	4.975IleLeu: 4.975 ± 0.065	1.204IleMet: 1.204 ± 0.029	1.594IleAsn: 1.594 ± 0.036	2.572IlePro: 2.572 ± 0.042	1.341IleGln: 1.341 ± 0.032	3.56IleArg: 3.56 ± 0.05	3.635IleSer: 3.635 ± 0.068	3.003IleThr: 3.003 ± 0.052	4.74IleVal: 4.74 ± 0.064	0.669IleTrp: 0.669 ± 0.022	1.287IleTyr: 1.287 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	4.764LysAla: 4.764 ± 0.063	0.152LysCys: 0.152 ± 0.01	2.095LysAsp: 2.095 ± 0.046	1.808LysGlu: 1.808 ± 0.04	1.11LysPhe: 1.11 ± 0.031	2.826LysGly: 2.826 ± 0.049	0.677LysHis: 0.677 ± 0.024	2.152LysIle: 2.152 ± 0.043	1.574LysLys: 1.574 ± 0.042	3.838LysLeu: 3.838 ± 0.055	0.986LysMet: 0.986 ± 0.027	1.126LysAsn: 1.126 ± 0.032	2.359LysPro: 2.359 ± 0.051	1.211LysGln: 1.211 ± 0.031	2.529LysArg: 2.529 ± 0.047	2.379LysSer: 2.379 ± 0.044	2.498LysThr: 2.498 ± 0.043	2.732LysVal: 2.732 ± 0.048	0.418LysTrp: 0.418 ± 0.016	0.683LysTyr: 0.683 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	12.068LeuAla: 12.068 ± 0.104	0.804LeuCys: 0.804 ± 0.026	5.936LeuAsp: 5.936 ± 0.065	5.163LeuGlu: 5.163 ± 0.066	3.751LeuPhe: 3.751 ± 0.065	7.931LeuGly: 7.931 ± 0.091	1.71LeuHis: 1.71 ± 0.034	5.344LeuIle: 5.344 ± 0.073	4.135LeuLys: 4.135 ± 0.058	9.116LeuLeu: 9.116 ± 0.115	2.502LeuMet: 2.502 ± 0.04	2.71LeuAsn: 2.71 ± 0.046	5.271LeuPro: 5.271 ± 0.071	2.805LeuGln: 2.805 ± 0.046	5.927LeuArg: 5.927 ± 0.076	7.572LeuSer: 7.572 ± 0.097	5.639LeuThr: 5.639 ± 0.073	7.38LeuVal: 7.38 ± 0.084	1.024LeuTrp: 1.024 ± 0.034	2.051LeuTyr: 2.051 ± 0.038	0.001LeuXaa: 0.001 ± 0.001
Met	3.16MetAla: 3.16 ± 0.049	0.119MetCys: 0.119 ± 0.01	1.303MetAsp: 1.303 ± 0.028	1.246MetGlu: 1.246 ± 0.028	0.796MetPhe: 0.796 ± 0.025	1.87MetGly: 1.87 ± 0.038	0.454MetHis: 0.454 ± 0.017	1.684MetIle: 1.684 ± 0.031	1.168MetLys: 1.168 ± 0.028	2.639MetLeu: 2.639 ± 0.044	0.779MetMet: 0.779 ± 0.025	0.847MetAsn: 0.847 ± 0.026	1.588MetPro: 1.588 ± 0.035	0.942MetGln: 0.942 ± 0.025	1.79MetArg: 1.79 ± 0.036	1.866MetSer: 1.866 ± 0.036	2.091MetThr: 2.091 ± 0.037	1.898MetVal: 1.898 ± 0.038	0.197MetTrp: 0.197 ± 0.013	0.329MetTyr: 0.329 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.301AsnAla: 3.301 ± 0.045	0.221AsnCys: 0.221 ± 0.014	1.615AsnAsp: 1.615 ± 0.041	1.381AsnGlu: 1.381 ± 0.033	1.114AsnPhe: 1.114 ± 0.029	2.624AsnGly: 2.624 ± 0.042	0.579AsnHis: 0.579 ± 0.021	1.533AsnIle: 1.533 ± 0.035	0.845AsnLys: 0.845 ± 0.023	2.738AsnLeu: 2.738 ± 0.048	0.697AsnMet: 0.697 ± 0.023	0.801AsnAsn: 0.801 ± 0.024	1.92AsnPro: 1.92 ± 0.039	0.83AsnGln: 0.83 ± 0.026	1.964AsnArg: 1.964 ± 0.039	1.5AsnSer: 1.5 ± 0.037	1.357AsnThr: 1.357 ± 0.029	2.05AsnVal: 2.05 ± 0.043	0.442AsnTrp: 0.442 ± 0.016	0.699AsnTyr: 0.699 ± 0.022	0.001AsnXaa: 0.001 ± 0.001
Pro	5.464ProAla: 5.464 ± 0.081	0.251ProCys: 0.251 ± 0.013	3.491ProAsp: 3.491 ± 0.05	3.406ProGlu: 3.406 ± 0.056	2.097ProPhe: 2.097 ± 0.042	4.035ProGly: 4.035 ± 0.06	1.019ProHis: 1.019 ± 0.026	2.468ProIle: 2.468 ± 0.046	1.896ProLys: 1.896 ± 0.037	4.416ProLeu: 4.416 ± 0.059	1.225ProMet: 1.225 ± 0.029	1.391ProAsn: 1.391 ± 0.032	2.053ProPro: 2.053 ± 0.047	1.614ProGln: 1.614 ± 0.035	2.432ProArg: 2.432 ± 0.052	2.915ProSer: 2.915 ± 0.047	2.437ProThr: 2.437 ± 0.042	4.142ProVal: 4.142 ± 0.061	0.576ProTrp: 0.576 ± 0.021	1.162ProTyr: 1.162 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.875GlnAla: 3.875 ± 0.059	0.164GlnCys: 0.164 ± 0.01	1.524GlnAsp: 1.524 ± 0.032	1.562GlnGlu: 1.562 ± 0.036	1.143GlnPhe: 1.143 ± 0.03	2.261GlnGly: 2.261 ± 0.048	0.567GlnHis: 0.567 ± 0.019	2.018GlnIle: 2.018 ± 0.058	1.282GlnLys: 1.282 ± 0.034	2.919GlnLeu: 2.919 ± 0.049	0.982GlnMet: 0.982 ± 0.028	0.917GlnAsn: 0.917 ± 0.025	1.757GlnPro: 1.757 ± 0.039	1.3GlnGln: 1.3 ± 0.039	2.197GlnArg: 2.197 ± 0.04	1.98GlnSer: 1.98 ± 0.04	1.818GlnThr: 1.818 ± 0.038	2.221GlnVal: 2.221 ± 0.049	0.366GlnTrp: 0.366 ± 0.018	0.634GlnTyr: 0.634 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	6.352ArgAla: 6.352 ± 0.072	0.406ArgCys: 0.406 ± 0.016	3.847ArgAsp: 3.847 ± 0.055	3.786ArgGlu: 3.786 ± 0.053	2.812ArgPhe: 2.812 ± 0.045	4.269ArgGly: 4.269 ± 0.062	1.531ArgHis: 1.531 ± 0.034	4.024ArgIle: 4.024 ± 0.059	2.433ArgLys: 2.433 ± 0.039	6.884ArgLeu: 6.884 ± 0.086	1.909ArgMet: 1.909 ± 0.038	1.961ArgAsn: 1.961 ± 0.038	2.989ArgPro: 2.989 ± 0.05	2.504ArgGln: 2.504 ± 0.04	4.793ArgArg: 4.793 ± 0.072	3.828ArgSer: 3.828 ± 0.056	3.174ArgThr: 3.174 ± 0.048	4.192ArgVal: 4.192 ± 0.057	0.813ArgTrp: 0.813 ± 0.025	1.696ArgTyr: 1.696 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.62SerAla: 6.62 ± 0.076	0.39SerCys: 0.39 ± 0.019	3.465SerAsp: 3.465 ± 0.058	3.253SerGlu: 3.253 ± 0.056	2.575SerPhe: 2.575 ± 0.037	6.103SerGly: 6.103 ± 0.069	1.2SerHis: 1.2 ± 0.027	3.403SerIle: 3.403 ± 0.048	2.072SerLys: 2.072 ± 0.043	6.041SerLeu: 6.041 ± 0.073	1.534SerMet: 1.534 ± 0.03	1.641SerAsn: 1.641 ± 0.034	2.857SerPro: 2.857 ± 0.046	1.894SerGln: 1.894 ± 0.042	3.871SerArg: 3.871 ± 0.054	3.821SerSer: 3.821 ± 0.07	3.193SerThr: 3.193 ± 0.06	4.603SerVal: 4.603 ± 0.055	0.755SerTrp: 0.755 ± 0.026	1.447SerTyr: 1.447 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	6.223ThrAla: 6.223 ± 0.074	0.395ThrCys: 0.395 ± 0.016	3.085ThrAsp: 3.085 ± 0.05	2.848ThrGlu: 2.848 ± 0.041	2.192ThrPhe: 2.192 ± 0.042	5.162ThrGly: 5.162 ± 0.069	1.003ThrHis: 1.003 ± 0.026	3.342ThrIle: 3.342 ± 0.05	1.777ThrLys: 1.777 ± 0.037	5.798ThrLeu: 5.798 ± 0.061	1.348ThrMet: 1.348 ± 0.03	1.465ThrAsn: 1.465 ± 0.031	3.008ThrPro: 3.008 ± 0.052	1.495ThrGln: 1.495 ± 0.036	3.062ThrArg: 3.062 ± 0.049	3.279ThrSer: 3.279 ± 0.054	3.032ThrThr: 3.032 ± 0.056	4.636ThrVal: 4.636 ± 0.056	0.666ThrTrp: 0.666 ± 0.022	1.291ThrTyr: 1.291 ± 0.031	0.001ThrXaa: 0.001 ± 0.001
Val	8.77ValAla: 8.77 ± 0.084	0.602ValCys: 0.602 ± 0.022	4.076ValAsp: 4.076 ± 0.054	4.533ValGlu: 4.533 ± 0.059	2.944ValPhe: 2.944 ± 0.052	5.479ValGly: 5.479 ± 0.074	1.361ValHis: 1.361 ± 0.033	4.443ValIle: 4.443 ± 0.056	2.668ValLys: 2.668 ± 0.05	7.333ValLeu: 7.333 ± 0.08	2.043ValMet: 2.043 ± 0.038	2.025ValAsn: 2.025 ± 0.043	3.525ValPro: 3.525 ± 0.05	2.026ValGln: 2.026 ± 0.037	4.456ValArg: 4.456 ± 0.054	5.024ValSer: 5.024 ± 0.062	4.727ValThr: 4.727 ± 0.065	5.955ValVal: 5.955 ± 0.071	0.803ValTrp: 0.803 ± 0.022	1.516ValTyr: 1.516 ± 0.032	0.001ValXaa: 0.001 ± 0.001
Trp	1.054TrpAla: 1.054 ± 0.031	0.124TrpCys: 0.124 ± 0.009	0.619TrpAsp: 0.619 ± 0.023	0.522TrpGlu: 0.522 ± 0.019	0.526TrpPhe: 0.526 ± 0.019	0.795TrpGly: 0.795 ± 0.024	0.274TrpHis: 0.274 ± 0.013	0.661TrpIle: 0.661 ± 0.021	0.513TrpLys: 0.513 ± 0.017	1.484TrpLeu: 1.484 ± 0.033	0.362TrpMet: 0.362 ± 0.015	0.463TrpAsn: 0.463 ± 0.018	0.636TrpPro: 0.636 ± 0.02	0.535TrpGln: 0.535 ± 0.018	0.926TrpArg: 0.926 ± 0.028	0.841TrpSer: 0.841 ± 0.024	0.727TrpThr: 0.727 ± 0.024	0.754TrpVal: 0.754 ± 0.021	0.198TrpTrp: 0.198 ± 0.013	0.278TrpTyr: 0.278 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.389TyrAla: 2.389 ± 0.037	0.226TyrCys: 0.226 ± 0.012	1.472TyrAsp: 1.472 ± 0.036	1.244TyrGlu: 1.244 ± 0.03	0.993TyrPhe: 0.993 ± 0.027	2.09TyrGly: 2.09 ± 0.037	0.483TyrHis: 0.483 ± 0.019	1.05TyrIle: 1.05 ± 0.03	0.722TyrLys: 0.722 ± 0.026	2.327TyrLeu: 2.327 ± 0.04	0.479TyrMet: 0.479 ± 0.023	0.645TyrAsn: 0.645 ± 0.024	1.125TyrPro: 1.125 ± 0.028	0.769TyrGln: 0.769 ± 0.025	1.678TyrArg: 1.678 ± 0.038	1.365TyrSer: 1.365 ± 0.027	1.141TyrThr: 1.141 ± 0.028	1.666TyrVal: 1.666 ± 0.035	0.363TyrTrp: 0.363 ± 0.016	0.65TyrTyr: 0.65 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4551 proteins (1439732 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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