Paenibacillus tyrfis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081NV69|A0A081NV69_9BACL Membrane protein OS=Paenibacillus tyrfis OX=1501230 GN=ET33_23475 PE=4 SV=1
MM1 pKa = 7.7PNKK4 pKa = 9.23FTEE7 pKa = 4.01TTYY10 pKa = 11.84ALNTCDD16 pKa = 4.22HH17 pKa = 6.85SSDD20 pKa = 3.32LLVYY24 pKa = 9.5FEE26 pKa = 5.62AIVMEE31 pKa = 4.37IQNGAAIKK39 pKa = 10.11DD40 pKa = 3.83YY41 pKa = 10.13QVCDD45 pKa = 3.59GSLVVTLQSDD55 pKa = 3.98DD56 pKa = 3.66VASS59 pKa = 3.57

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081P887|A0A081P887_9BACL Amidase_6 domain-containing protein OS=Paenibacillus tyrfis OX=1501230 GN=ET33_29680 PE=4 SV=1
MM1 pKa = 7.66LLHH4 pKa = 7.0LFRR7 pKa = 11.84FRR9 pKa = 11.84FRR11 pKa = 11.84LLWLRR16 pKa = 11.84WLRR19 pKa = 11.84WFPLRR24 pKa = 11.84WLRR27 pKa = 11.84LRR29 pKa = 11.84WLFPLLWLRR38 pKa = 11.84WFPLRR43 pKa = 11.84WFPLWVPLRR52 pKa = 11.84PVRR55 pKa = 11.84SVLLMFQQ62 pKa = 3.41

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6455

0

6455

2041611

33

8521

316.3

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.715 ± 0.038

0.814 ± 0.01

5.009 ± 0.022

6.764 ± 0.033

4.139 ± 0.022

7.491 ± 0.028

2.111 ± 0.014

6.202 ± 0.028

5.369 ± 0.034

10.336 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.013

3.451 ± 0.023

4.261 ± 0.02

3.883 ± 0.023

5.47 ± 0.029

5.987 ± 0.023

5.32 ± 0.023

7.225 ± 0.023

1.279 ± 0.012

3.433 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski