Streptomyces phage phiC31 (Bacteriophage phi-C31)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lomovskayavirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9ZX86|Q9ZX86_BPPHC Gp22 OS=Streptomyces phage phiC31 OX=10719 GN=22 PE=4 SV=1
MM1 pKa = 7.61PNKK4 pKa = 8.94ITFGASVLASAATAFGLGALAFTSPNAPEE33 pKa = 3.89TAYY36 pKa = 9.81PLPTHH41 pKa = 6.47SAPDD45 pKa = 3.89VEE47 pKa = 5.3PEE49 pKa = 4.0TAPLSVGATEE59 pKa = 5.55DD60 pKa = 3.6PGTVPTIAPVACTAPSSTPKK80 pKa = 9.72TGRR83 pKa = 11.84HH84 pKa = 4.78SKK86 pKa = 10.23PRR88 pKa = 11.84TEE90 pKa = 4.79PEE92 pKa = 3.8TDD94 pKa = 3.32DD95 pKa = 3.55TATLPRR101 pKa = 11.84HH102 pKa = 6.22AKK104 pKa = 9.82PLATPSPSSTTPAGSKK120 pKa = 10.98GPLTDD125 pKa = 4.81AEE127 pKa = 4.75DD128 pKa = 4.06DD129 pKa = 4.21GKK131 pKa = 11.49VLDD134 pKa = 5.5DD135 pKa = 5.39LGADD139 pKa = 3.89LPLPDD144 pKa = 4.52VGISIPDD151 pKa = 3.3VMLPFPGHH159 pKa = 4.94NHH161 pKa = 5.71EE162 pKa = 5.39ADD164 pKa = 3.4AEE166 pKa = 4.5TPLIAEE172 pKa = 4.92GGSPEE177 pKa = 4.08YY178 pKa = 9.9PYY180 pKa = 11.25EE181 pKa = 4.22EE182 pKa = 4.33AWGDD186 pKa = 3.93TPEE189 pKa = 4.09TAADD193 pKa = 3.83PGVVCDD199 pKa = 5.06KK200 pKa = 10.8EE201 pKa = 3.99LAEE204 pKa = 4.44ALGVPYY210 pKa = 10.66SPTCYY215 pKa = 9.24TGHH218 pKa = 6.41SPADD222 pKa = 3.0TWARR226 pKa = 11.84EE227 pKa = 3.84EE228 pKa = 5.22AEE230 pKa = 5.63ADD232 pKa = 3.79DD233 pKa = 4.6QPAGPVWDD241 pKa = 4.18LSGGNGWFF249 pKa = 3.54

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9ZX94|Q9ZX94_BPPHC Gp14 OS=Streptomyces phage phiC31 OX=10719 GN=14 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84VTTEE6 pKa = 4.13TKK8 pKa = 10.77AEE10 pKa = 3.99LQAEE14 pKa = 4.61RR15 pKa = 11.84GRR17 pKa = 11.84VVGFVKK23 pKa = 10.45SSEE26 pKa = 3.99PGKK29 pKa = 10.61ISIAVPADD37 pKa = 3.75RR38 pKa = 11.84AVMTPAQARR47 pKa = 11.84QLAAWLNEE55 pKa = 3.65EE56 pKa = 4.16ADD58 pKa = 3.46RR59 pKa = 11.84GGRR62 pKa = 11.84VSTDD66 pKa = 2.31ARR68 pKa = 11.84TAEE71 pKa = 4.1GWRR74 pKa = 11.84TAEE77 pKa = 4.63AEE79 pKa = 3.93RR80 pKa = 11.84QRR82 pKa = 11.84AIRR85 pKa = 11.84EE86 pKa = 4.01ALNVDD91 pKa = 3.81RR92 pKa = 11.84VTTPSGRR99 pKa = 11.84TFTRR103 pKa = 11.84RR104 pKa = 11.84AYY106 pKa = 10.37

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12454

41

805

235.0

25.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.357 ± 0.396

0.988 ± 0.149

6.913 ± 0.269

6.054 ± 0.349

3.123 ± 0.205

8.76 ± 0.41

1.718 ± 0.159

3.525 ± 0.221

4.424 ± 0.361

7.676 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.031 ± 0.136

2.875 ± 0.196

5.412 ± 0.357

2.409 ± 0.122

6.825 ± 0.423

5.484 ± 0.229

6.769 ± 0.365

7.917 ± 0.286

1.959 ± 0.168

2.778 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski