Mosquito VEM Anellovirus SDBVL A

Taxonomy: Viruses; Anelloviridae; unclassified Anelloviridae

Average proteome isoelectric point is 9.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6KID0|F6KID0_9VIRU Uncharacterized protein OS=Mosquito VEM Anellovirus SDBVL A OX=1034790 PE=4 SV=1
MM1 pKa = 7.54ALYY4 pKa = 9.95FRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84TWRR13 pKa = 11.84PRR15 pKa = 11.84WRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84GWWRR23 pKa = 11.84RR24 pKa = 11.84SYY26 pKa = 10.64RR27 pKa = 11.84SAYY30 pKa = 8.58GRR32 pKa = 11.84RR33 pKa = 11.84FRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84TRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SRR45 pKa = 11.84VRR47 pKa = 11.84VRR49 pKa = 11.84VLTEE53 pKa = 3.47RR54 pKa = 11.84QPRR57 pKa = 11.84YY58 pKa = 8.33WRR60 pKa = 11.84RR61 pKa = 11.84CTIRR65 pKa = 11.84GWWPVLITGRR75 pKa = 11.84KK76 pKa = 7.55YY77 pKa = 10.69PSTAAQGDD85 pKa = 3.93DD86 pKa = 4.53AGTQMLTFKK95 pKa = 10.27PFQPFGSKK103 pKa = 10.44LSNAWFYY110 pKa = 11.36RR111 pKa = 11.84DD112 pKa = 3.61YY113 pKa = 11.13EE114 pKa = 4.19IVLGGFSAGTFSLQALYY131 pKa = 9.63MEE133 pKa = 4.61HH134 pKa = 7.81LMYY137 pKa = 10.7RR138 pKa = 11.84NRR140 pKa = 11.84WSQSNCGFDD149 pKa = 3.28LASYY153 pKa = 10.31RR154 pKa = 11.84GTKK157 pKa = 10.1LYY159 pKa = 10.27FLPHH163 pKa = 6.78PNYY166 pKa = 10.77DD167 pKa = 4.13YY168 pKa = 11.13IVLIDD173 pKa = 3.4PEE175 pKa = 4.31YY176 pKa = 11.01RR177 pKa = 11.84KK178 pKa = 10.14FEE180 pKa = 3.91QWIKK184 pKa = 10.76QPMHH188 pKa = 6.55PGVLITHH195 pKa = 6.17PQSRR199 pKa = 11.84IIRR202 pKa = 11.84SISHH206 pKa = 6.71AGPRR210 pKa = 11.84RR211 pKa = 11.84KK212 pKa = 8.76MPKK215 pKa = 8.94MFVPPPSTMNSGWQWMGEE233 pKa = 4.03LANEE237 pKa = 4.3GLFAFYY243 pKa = 10.2VQWIDD248 pKa = 5.72LGAPWLGDD256 pKa = 3.3VQNPNQVKK264 pKa = 8.2WWKK267 pKa = 9.23TGDD270 pKa = 3.42ATTKK274 pKa = 10.03PDD276 pKa = 3.3WVTKK280 pKa = 10.07SIEE283 pKa = 4.2MQSKK287 pKa = 7.61TVQEE291 pKa = 4.43GLDD294 pKa = 3.53TYY296 pKa = 10.63YY297 pKa = 11.23SRR299 pKa = 11.84AGTGDD304 pKa = 3.97KK305 pKa = 10.56KK306 pKa = 10.24WVDD309 pKa = 3.81PGSAPYY315 pKa = 10.41QLGFGPFVFKK325 pKa = 10.8GCHH328 pKa = 5.81TGDD331 pKa = 3.48QEE333 pKa = 4.91HH334 pKa = 6.51YY335 pKa = 8.96PQITFFYY342 pKa = 10.08KK343 pKa = 10.48SYY345 pKa = 8.07WQWGGSTTSIKK356 pKa = 9.26TVCDD360 pKa = 3.6PKK362 pKa = 11.21DD363 pKa = 3.68NPNAEE368 pKa = 4.48YY369 pKa = 10.67KK370 pKa = 10.42SAWNRR375 pKa = 11.84AGFKK379 pKa = 10.32PEE381 pKa = 3.54QARR384 pKa = 11.84NSDD387 pKa = 4.05IYY389 pKa = 10.76NQPP392 pKa = 2.97

Molecular weight: 46.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6KID0|F6KID0_9VIRU Uncharacterized protein OS=Mosquito VEM Anellovirus SDBVL A OX=1034790 PE=4 SV=1
MM1 pKa = 7.35QNTNRR6 pKa = 11.84PGIGLGLNLSRR17 pKa = 11.84PEE19 pKa = 3.61IVTFIINHH27 pKa = 5.47RR28 pKa = 11.84PKK30 pKa = 9.94VTFDD34 pKa = 3.76LSPPEE39 pKa = 4.34LEE41 pKa = 4.22PVQTSTQTRR50 pKa = 11.84SGRR53 pKa = 11.84PLKK56 pKa = 10.34RR57 pKa = 11.84RR58 pKa = 11.84SIVRR62 pKa = 11.84MQKK65 pKa = 6.99TTTSGGSSIQTPFKK79 pKa = 10.73EE80 pKa = 4.16LLNKK84 pKa = 10.35LSEE87 pKa = 4.41SQCRR91 pKa = 11.84QLASALFPKK100 pKa = 10.24SEE102 pKa = 3.97SRR104 pKa = 11.84ATMRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84SMLPRR119 pKa = 11.84RR120 pKa = 11.84KK121 pKa = 8.84RR122 pKa = 11.84TRR124 pKa = 11.84EE125 pKa = 3.86HH126 pKa = 5.99EE127 pKa = 4.18TDD129 pKa = 3.16SDD131 pKa = 3.57EE132 pKa = 5.52GSFGDD137 pKa = 3.45SGSYY141 pKa = 9.91SSRR144 pKa = 11.84GGGKK148 pKa = 9.87RR149 pKa = 11.84SGSEE153 pKa = 3.8DD154 pKa = 3.35TSGSEE159 pKa = 4.33SSSGLDD165 pKa = 5.14DD166 pKa = 5.91PDD168 pKa = 3.98TEE170 pKa = 4.35QNYY173 pKa = 9.36PPVRR177 pKa = 11.84TRR179 pKa = 11.84LAPIKK184 pKa = 10.09FSKK187 pKa = 10.31DD188 pKa = 3.33CRR190 pKa = 11.84PLGPWMM196 pKa = 4.66

Molecular weight: 21.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

588

196

392

294.0

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.082 ± 1.083

1.02 ± 0.0

4.422 ± 0.09

4.082 ± 1.083

4.422 ± 0.722

7.993 ± 0.09

1.531 ± 0.271

3.741 ± 0.09

5.272 ± 0.09

5.952 ± 0.902

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.0

3.231 ± 0.09

7.483 ± 0.361

4.932 ± 0.451

11.905 ± 0.722

9.014 ± 2.797

6.803 ± 0.722

3.571 ± 0.541

3.741 ± 1.714

4.252 ± 1.714
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski