[Clostridium] bolteae 90A9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Enterocloster; Enterocloster bolteae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5741 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R0BW05|R0BW05_9FIRM Probable membrane transporter protein OS=[Clostridium] bolteae 90A9 OX=997894 GN=HMPREF1085_03594 PE=3 SV=1
MM1 pKa = 7.93GYY3 pKa = 10.11QDD5 pKa = 4.93DD6 pKa = 4.41YY7 pKa = 11.98VMRR10 pKa = 11.84TISDD14 pKa = 3.92LVRR17 pKa = 11.84AIARR21 pKa = 11.84LVLGKK26 pKa = 10.31SDD28 pKa = 3.42IDD30 pKa = 3.68YY31 pKa = 10.82DD32 pKa = 4.49LPEE35 pKa = 6.63DD36 pKa = 3.81EE37 pKa = 6.37DD38 pKa = 5.06KK39 pKa = 11.19YY40 pKa = 9.41TDD42 pKa = 3.64LDD44 pKa = 3.59RR45 pKa = 11.84VYY47 pKa = 11.05KK48 pKa = 10.19RR49 pKa = 11.84LKK51 pKa = 10.84DD52 pKa = 3.75LVDD55 pKa = 3.69EE56 pKa = 4.79GNINDD61 pKa = 4.74AEE63 pKa = 4.11NLLTDD68 pKa = 4.28EE69 pKa = 5.68LDD71 pKa = 3.83TDD73 pKa = 4.42SLDD76 pKa = 4.0CLEE79 pKa = 4.54MALTFYY85 pKa = 10.39MYY87 pKa = 11.04LNQLKK92 pKa = 10.76DD93 pKa = 3.61EE94 pKa = 4.47EE95 pKa = 5.71LYY97 pKa = 8.74TANYY101 pKa = 8.9SRR103 pKa = 11.84EE104 pKa = 4.08EE105 pKa = 3.92IVDD108 pKa = 4.75GINSVCAEE116 pKa = 3.9YY117 pKa = 10.48GISGFEE123 pKa = 4.16HH124 pKa = 6.61FVDD127 pKa = 3.69TTMVV131 pKa = 2.98

Molecular weight: 15.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R0AGY4|R0AGY4_9FIRM G5 domain-containing protein OS=[Clostridium] bolteae 90A9 OX=997894 GN=HMPREF1085_01534 PE=4 SV=1
MM1 pKa = 7.48NFTDD5 pKa = 3.92SPYY8 pKa = 10.28EE9 pKa = 3.96RR10 pKa = 11.84MMKK13 pKa = 9.62QVPRR17 pKa = 11.84PSRR20 pKa = 11.84HH21 pKa = 6.45DD22 pKa = 3.81PEE24 pKa = 5.0PCPACRR30 pKa = 11.84EE31 pKa = 4.08RR32 pKa = 11.84GGDD35 pKa = 3.51CRR37 pKa = 11.84KK38 pKa = 9.88KK39 pKa = 10.06RR40 pKa = 11.84RR41 pKa = 11.84TLIVEE46 pKa = 4.22PRR48 pKa = 11.84RR49 pKa = 11.84TSGQVGPKK57 pKa = 9.68RR58 pKa = 3.55

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5741

0

5741

1888569

24

4311

329.0

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.064 ± 0.034

1.585 ± 0.014

5.607 ± 0.027

7.181 ± 0.035

3.968 ± 0.022

7.927 ± 0.035

1.759 ± 0.015

6.802 ± 0.031

5.693 ± 0.022

9.056 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.426 ± 0.017

3.805 ± 0.02

3.647 ± 0.02

3.385 ± 0.02

5.102 ± 0.03

5.861 ± 0.023

5.25 ± 0.026

6.915 ± 0.024

1.014 ± 0.012

3.952 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski