Capybara microvirus Cap3_SP_465

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W588|A0A4P8W588_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_465 OX=2585462 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.18IEE4 pKa = 4.5FKK6 pKa = 11.13NPLEE10 pKa = 4.96IDD12 pKa = 3.27TSSYY16 pKa = 11.39VSVCGLPYY24 pKa = 10.61EE25 pKa = 5.4DD26 pKa = 6.06DD27 pKa = 3.99YY28 pKa = 12.05QLSLDD33 pKa = 4.53DD34 pKa = 5.3NGCEE38 pKa = 3.96YY39 pKa = 10.71LKK41 pKa = 10.74KK42 pKa = 10.62VGVIEE47 pKa = 4.38TYY49 pKa = 11.14KK50 pKa = 10.65LIQSHH55 pKa = 6.97RR56 pKa = 11.84DD57 pKa = 3.42SCDD60 pKa = 2.78LAYY63 pKa = 9.8ILDD66 pKa = 4.04TLDD69 pKa = 4.08PDD71 pKa = 4.01SVLGSGSPNVSFDD84 pKa = 4.64DD85 pKa = 4.14IYY87 pKa = 11.05IDD89 pKa = 3.7FTKK92 pKa = 10.63FPTNPGEE99 pKa = 4.13AFNLIKK105 pKa = 10.72NAEE108 pKa = 3.99FAFNKK113 pKa = 10.34LPFEE117 pKa = 4.74LRR119 pKa = 11.84QEE121 pKa = 4.75CNFSPNVFVKK131 pKa = 10.76NLDD134 pKa = 3.35KK135 pKa = 11.04LAEE138 pKa = 4.32KK139 pKa = 10.31FSKK142 pKa = 9.87VDD144 pKa = 3.12KK145 pKa = 10.5VEE147 pKa = 4.2SVGVSNEE154 pKa = 3.56

Molecular weight:
17.4 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P8W543|A0A4P8W543_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_465 OX=2585462 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84SILEE6 pKa = 4.2ASQSPNNWFITLTYY20 pKa = 10.94DD21 pKa = 4.06NDD23 pKa = 3.77HH24 pKa = 6.64LPISKK29 pKa = 10.06YY30 pKa = 10.62LNEE33 pKa = 4.04EE34 pKa = 3.86TGVYY38 pKa = 9.83TEE40 pKa = 5.04TPTLDD45 pKa = 3.39NVEE48 pKa = 4.06EE49 pKa = 4.2MKK51 pKa = 10.96KK52 pKa = 8.46FWKK55 pKa = 10.06RR56 pKa = 11.84FRR58 pKa = 11.84INLSRR63 pKa = 11.84SSDD66 pKa = 3.04VDD68 pKa = 3.75LKK70 pKa = 11.28VRR72 pKa = 11.84YY73 pKa = 8.89LYY75 pKa = 10.87SGEE78 pKa = 4.34YY79 pKa = 10.59GDD81 pKa = 3.96TTHH84 pKa = 7.29RR85 pKa = 11.84PHH87 pKa = 6.41FHH89 pKa = 7.26AILFNTVLPDD99 pKa = 3.62LKK101 pKa = 10.99VFYY104 pKa = 10.14KK105 pKa = 10.68DD106 pKa = 2.96KK107 pKa = 10.23QTGFTYY113 pKa = 9.3YY114 pKa = 10.26TSEE117 pKa = 4.37LLEE120 pKa = 4.37KK121 pKa = 10.0SWKK124 pKa = 9.09KK125 pKa = 11.02GNVLIADD132 pKa = 4.27CTFEE136 pKa = 3.95TCQYY140 pKa = 8.4VAAYY144 pKa = 9.24VVKK147 pKa = 10.34KK148 pKa = 7.93QTGKK152 pKa = 10.27QSQVYY157 pKa = 9.9DD158 pKa = 4.17DD159 pKa = 4.39VNIIPPYY166 pKa = 7.81MQMSRR171 pKa = 11.84RR172 pKa = 11.84PGIGSQVIEE181 pKa = 4.58LNLEE185 pKa = 4.4TFWHH189 pKa = 6.87DD190 pKa = 3.17KK191 pKa = 11.03SIILSTKK198 pKa = 10.44NGGKK202 pKa = 9.01QLPFDD207 pKa = 3.68QYY209 pKa = 11.45IKK211 pKa = 11.25NKK213 pKa = 9.85LDD215 pKa = 3.31KK216 pKa = 11.12QFGEE220 pKa = 4.88EE221 pKa = 3.86YY222 pKa = 10.39QEE224 pKa = 4.51YY225 pKa = 10.62KK226 pKa = 10.88NQLCEE231 pKa = 4.67DD232 pKa = 3.6MKK234 pKa = 10.95EE235 pKa = 4.12LQEE238 pKa = 4.1LKK240 pKa = 10.77YY241 pKa = 11.0GSLEE245 pKa = 3.91KK246 pKa = 10.18TYY248 pKa = 10.93LQALLDD254 pKa = 3.74EE255 pKa = 4.79EE256 pKa = 5.71ANFKK260 pKa = 11.14ANYY263 pKa = 8.55FKK265 pKa = 11.25NKK267 pKa = 8.73SRR269 pKa = 11.84KK270 pKa = 8.87LL271 pKa = 3.53

Molecular weight:
31.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1463

82

561

243.8

27.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.177 ± 2.799

1.025 ± 0.296

6.083 ± 0.837

4.99 ± 1.007

4.785 ± 0.849

6.015 ± 0.77

1.846 ± 0.589

4.785 ± 0.387

5.947 ± 1.433

8.271 ± 0.895

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.329

6.63 ± 1.038

3.623 ± 0.865

4.238 ± 0.741

3.008 ± 0.524

10.321 ± 1.578

6.015 ± 0.707

6.425 ± 0.675

1.025 ± 0.423

5.4 ± 0.554

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski