Sarcina sp. DSM 11001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Sarcina; unclassified Sarcina

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9F2P0|A0A1G9F2P0_9CLOT TPM_phosphatase domain-containing protein OS=Sarcina sp. DSM 11001 OX=1798184 GN=SAMN04487833_1085 PE=4 SV=1
MM1 pKa = 7.39HH2 pKa = 7.73KK3 pKa = 10.14NRR5 pKa = 11.84KK6 pKa = 8.93RR7 pKa = 11.84FQALVLAAAITLAAVPGTGFTARR30 pKa = 11.84AAEE33 pKa = 4.08PEE35 pKa = 4.31IPAEE39 pKa = 3.77TAYY42 pKa = 9.88PLEE45 pKa = 4.34PEE47 pKa = 4.45IPAEE51 pKa = 3.72TAYY54 pKa = 9.88PLEE57 pKa = 4.34PEE59 pKa = 4.45IPAEE63 pKa = 3.78TAYY66 pKa = 9.94PSEE69 pKa = 4.31PEE71 pKa = 4.0IPAEE75 pKa = 3.67TAYY78 pKa = 9.98PLEE81 pKa = 4.92AEE83 pKa = 4.37EE84 pKa = 4.19TAADD88 pKa = 3.84AVDD91 pKa = 6.11GIALPAVSLNQEE103 pKa = 3.64EE104 pKa = 5.32SVFGEE109 pKa = 4.23IPAEE113 pKa = 4.04DD114 pKa = 4.76EE115 pKa = 4.23IEE117 pKa = 4.09EE118 pKa = 4.83DD119 pKa = 3.42STNPEE124 pKa = 4.35DD125 pKa = 4.55EE126 pKa = 4.59ILPEE130 pKa = 4.57GDD132 pKa = 3.84SPDD135 pKa = 3.33IQEE138 pKa = 4.97DD139 pKa = 4.12VSSEE143 pKa = 4.03PTGGTVSQEE152 pKa = 4.01NPEE155 pKa = 3.98EE156 pKa = 4.35DD157 pKa = 3.63SFPEE161 pKa = 3.98NPEE164 pKa = 3.74EE165 pKa = 5.09KK166 pKa = 10.51GAAEE170 pKa = 5.4NPDD173 pKa = 2.79QDD175 pKa = 3.73AASEE179 pKa = 4.23ITEE182 pKa = 4.18EE183 pKa = 4.11TDD185 pKa = 3.24PAEE188 pKa = 4.66TPDD191 pKa = 3.63QDD193 pKa = 3.83VASEE197 pKa = 4.04NLEE200 pKa = 3.9EE201 pKa = 4.04TDD203 pKa = 3.16AQNFPEE209 pKa = 4.57EE210 pKa = 4.35NTSEE214 pKa = 4.2EE215 pKa = 4.29PAEE218 pKa = 4.36GAAQEE223 pKa = 4.65TEE225 pKa = 4.28TVTQSVDD232 pKa = 3.13EE233 pKa = 4.31VRR235 pKa = 11.84EE236 pKa = 4.09KK237 pKa = 10.65TDD239 pKa = 4.11VEE241 pKa = 4.84HH242 pKa = 7.38EE243 pKa = 4.4DD244 pKa = 3.3NDD246 pKa = 4.56LLFEE250 pKa = 4.66EE251 pKa = 4.87YY252 pKa = 10.85AEE254 pKa = 4.57AMFEE258 pKa = 4.03EE259 pKa = 5.09AADD262 pKa = 4.13GAQPGTSGRR271 pKa = 11.84GQKK274 pKa = 8.24NTGDD278 pKa = 3.44QLTGQDD284 pKa = 3.09RR285 pKa = 11.84VIYY288 pKa = 10.02DD289 pKa = 3.65ALKK292 pKa = 10.53AAALEE297 pKa = 4.3IADD300 pKa = 4.17GQRR303 pKa = 11.84DD304 pKa = 3.78STIVEE309 pKa = 4.13IPLSEE314 pKa = 4.4LGIDD318 pKa = 3.75AGKK321 pKa = 10.57QYY323 pKa = 9.58TAEE326 pKa = 4.67DD327 pKa = 3.64LGLDD331 pKa = 3.58YY332 pKa = 10.49IYY334 pKa = 10.92RR335 pKa = 11.84RR336 pKa = 11.84VNGTGEE342 pKa = 4.13WNPAIEE348 pKa = 4.39DD349 pKa = 3.86AVNSMLSFDD358 pKa = 3.29SRR360 pKa = 11.84EE361 pKa = 3.53IFRR364 pKa = 11.84RR365 pKa = 11.84LWADD369 pKa = 3.6CPYY372 pKa = 11.21EE373 pKa = 4.15MYY375 pKa = 8.71WQKK378 pKa = 11.07GGYY381 pKa = 8.69SFPVSLGCSKK391 pKa = 10.51RR392 pKa = 11.84ASGSGDD398 pKa = 2.8SWSGFVVIEE407 pKa = 3.93EE408 pKa = 4.52SPVTFSMCVEE418 pKa = 3.64QKK420 pKa = 10.49YY421 pKa = 9.03RR422 pKa = 11.84QDD424 pKa = 3.23QDD426 pKa = 4.28DD427 pKa = 4.76EE428 pKa = 5.37YY429 pKa = 11.78SVDD432 pKa = 3.49TSKK435 pKa = 9.75TGAACSAALFARR447 pKa = 11.84SIVNDD452 pKa = 3.72ADD454 pKa = 3.54NSGLSDD460 pKa = 3.76YY461 pKa = 11.43DD462 pKa = 3.3RR463 pKa = 11.84LVYY466 pKa = 10.79YY467 pKa = 10.41KK468 pKa = 10.72DD469 pKa = 4.03RR470 pKa = 11.84ICNEE474 pKa = 3.43VVYY477 pKa = 10.99NEE479 pKa = 4.2EE480 pKa = 3.76ARR482 pKa = 11.84QNSEE486 pKa = 4.26TYY488 pKa = 9.87PDD490 pKa = 3.71RR491 pKa = 11.84GPWALIYY498 pKa = 11.08VFDD501 pKa = 4.52QNPDD505 pKa = 3.43TNVVCEE511 pKa = 4.79GYY513 pKa = 10.22SEE515 pKa = 4.36AFQYY519 pKa = 11.24LCGLTEE525 pKa = 4.02FQSRR529 pKa = 11.84KK530 pKa = 7.39IQVYY534 pKa = 9.47SVSGMFGGGTGAGPHH549 pKa = 6.23KK550 pKa = 10.27WNIVHH555 pKa = 6.79MDD557 pKa = 3.54DD558 pKa = 4.39GLNYY562 pKa = 10.45LADD565 pKa = 3.64VTNSDD570 pKa = 4.15EE571 pKa = 4.66GTVGWDD577 pKa = 2.89GRR579 pKa = 11.84LFLKK583 pKa = 10.79GIEE586 pKa = 4.1GNVYY590 pKa = 10.49DD591 pKa = 5.06GYY593 pKa = 9.89VLSWEE598 pKa = 4.47EE599 pKa = 3.7YY600 pKa = 7.98QEE602 pKa = 4.13EE603 pKa = 4.49VEE605 pKa = 4.91EE606 pKa = 4.09EE607 pKa = 4.18DD608 pKa = 3.04GTYY611 pKa = 10.79VYY613 pKa = 9.21TYY615 pKa = 9.64PAGSVDD621 pKa = 3.48YY622 pKa = 10.67AYY624 pKa = 10.53DD625 pKa = 3.37QEE627 pKa = 4.25TRR629 pKa = 11.84ALFTDD634 pKa = 3.82EE635 pKa = 5.13EE636 pKa = 4.71LTLSSGDD643 pKa = 3.87YY644 pKa = 11.04VPGSLPPGPPAPTVDD659 pKa = 3.99EE660 pKa = 4.11IVSIEE665 pKa = 4.11EE666 pKa = 4.05VYY668 pKa = 10.76LYY670 pKa = 9.75LTDD673 pKa = 4.58KK674 pKa = 11.18IEE676 pKa = 3.97MRR678 pKa = 11.84FCAKK682 pKa = 10.37VEE684 pKa = 4.21GNIEE688 pKa = 3.76QDD690 pKa = 3.29DD691 pKa = 5.01YY692 pKa = 11.58ITFICAGRR700 pKa = 11.84TVTQTVSEE708 pKa = 4.68AEE710 pKa = 4.01TDD712 pKa = 3.57SEE714 pKa = 4.23GRR716 pKa = 11.84LVFSLEE722 pKa = 3.52LAARR726 pKa = 11.84QMTDD730 pKa = 2.32QVTYY734 pKa = 10.76FMTVDD739 pKa = 3.94GTAGTSGQYY748 pKa = 9.3SVRR751 pKa = 11.84SYY753 pKa = 11.94ADD755 pKa = 3.32TILNQSGMRR764 pKa = 11.84DD765 pKa = 3.45YY766 pKa = 11.33KK767 pKa = 11.08GLLRR771 pKa = 11.84AMLNYY776 pKa = 10.39GSYY779 pKa = 7.79TQLYY783 pKa = 8.47AGYY786 pKa = 8.05RR787 pKa = 11.84TDD789 pKa = 4.57FLAAEE794 pKa = 4.35GLYY797 pKa = 10.02TDD799 pKa = 3.92EE800 pKa = 4.73TDD802 pKa = 3.38PVLNMEE808 pKa = 4.96GPDD811 pKa = 3.67LTDD814 pKa = 3.85FAYY817 pKa = 9.42TYY819 pKa = 10.97KK820 pKa = 10.83LNSKK824 pKa = 9.1TDD826 pKa = 3.01GLTIGKK832 pKa = 10.1ASLLLGTDD840 pKa = 3.91LSLRR844 pKa = 11.84LYY846 pKa = 10.36YY847 pKa = 10.47EE848 pKa = 4.32PGEE851 pKa = 4.35GKK853 pKa = 8.54TDD855 pKa = 3.11KK856 pKa = 10.95SYY858 pKa = 11.4SIVLADD864 pKa = 4.08RR865 pKa = 11.84SGLEE869 pKa = 3.93PVLGYY874 pKa = 10.96DD875 pKa = 3.89DD876 pKa = 4.9TIGMYY881 pKa = 10.33YY882 pKa = 10.81ADD884 pKa = 4.88IEE886 pKa = 5.19HH887 pKa = 6.71ITPTQIGDD895 pKa = 3.47MFKK898 pKa = 11.01LDD900 pKa = 4.94FYY902 pKa = 11.82AEE904 pKa = 4.6DD905 pKa = 4.0GQAADD910 pKa = 4.07TPAASVTFGPLSYY923 pKa = 10.64CKK925 pKa = 10.32GVLEE929 pKa = 4.95SGSSSDD935 pKa = 4.07EE936 pKa = 3.95LVNLCRR942 pKa = 11.84ALYY945 pKa = 9.31EE946 pKa = 3.9YY947 pKa = 9.5WQIAIRR953 pKa = 11.84PLTT956 pKa = 3.64

Molecular weight: 105.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9K7T8|A0A1G9K7T8_9CLOT Prokaryotic molybdopterin-containing oxidoreductase family iron-sulfur binding subunit OS=Sarcina sp. DSM 11001 OX=1798184 GN=SAMN04487833_1205 PE=4 SV=1
MM1 pKa = 7.7AKK3 pKa = 8.05MTFQPKK9 pKa = 9.04KK10 pKa = 7.6RR11 pKa = 11.84QRR13 pKa = 11.84AKK15 pKa = 9.8VHH17 pKa = 5.81GFLSRR22 pKa = 11.84MSTKK26 pKa = 10.3GGRR29 pKa = 11.84KK30 pKa = 8.75VLSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.45GRR40 pKa = 11.84KK41 pKa = 8.8QIAVV45 pKa = 3.36

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3187

0

3187

1153430

35

3547

361.9

40.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.266 ± 0.054

1.474 ± 0.018

6.181 ± 0.038

7.652 ± 0.052

3.942 ± 0.031

7.638 ± 0.049

1.743 ± 0.016

6.482 ± 0.039

5.655 ± 0.044

8.555 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.779 ± 0.025

3.863 ± 0.03

3.862 ± 0.027

3.079 ± 0.023

5.521 ± 0.047

6.265 ± 0.038

5.743 ± 0.047

6.499 ± 0.036

0.954 ± 0.015

3.848 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski