Amino acid dipepetide frequency for Lactococcus virus bIL67

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.151AlaCys: 0.151 ± 0.159
3.934AlaAsp: 3.934 ± 0.624
4.993AlaGlu: 4.993 ± 1.073
2.118AlaPhe: 2.118 ± 0.466
2.875AlaGly: 2.875 ± 0.628
0.151AlaHis: 0.151 ± 0.162
4.388AlaIle: 4.388 ± 0.753
5.598AlaLys: 5.598 ± 0.814
7.112AlaLeu: 7.112 ± 1.377
1.664AlaMet: 1.664 ± 0.361
4.388AlaAsn: 4.388 ± 0.831
0.908AlaPro: 0.908 ± 0.359
2.875AlaGln: 2.875 ± 1.006
1.967AlaArg: 1.967 ± 0.613
4.539AlaSer: 4.539 ± 0.784
4.388AlaThr: 4.388 ± 1.079
4.237AlaVal: 4.237 ± 1.224
0.757AlaTrp: 0.757 ± 0.312
3.631AlaTyr: 3.631 ± 0.753
0.0AlaXaa: 0.0 ± 0.0
Cys
0.303CysAla: 0.303 ± 0.187
0.303CysCys: 0.303 ± 0.207
0.303CysAsp: 0.303 ± 0.207
0.303CysGlu: 0.303 ± 0.183
0.303CysPhe: 0.303 ± 0.237
0.757CysGly: 0.757 ± 0.315
0.0CysHis: 0.0 ± 0.0
0.151CysIle: 0.151 ± 0.145
0.908CysLys: 0.908 ± 0.304
1.059CysLeu: 1.059 ± 0.543
0.151CysMet: 0.151 ± 0.171
0.454CysAsn: 0.454 ± 0.246
0.0CysPro: 0.0 ± 0.0
0.303CysGln: 0.303 ± 0.182
0.454CysArg: 0.454 ± 0.287
0.605CysSer: 0.605 ± 0.322
0.605CysThr: 0.605 ± 0.288
0.151CysVal: 0.151 ± 0.123
0.0CysTrp: 0.0 ± 0.0
0.151CysTyr: 0.151 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
2.572AspAla: 2.572 ± 0.529
0.303AspCys: 0.303 ± 0.23
3.177AspAsp: 3.177 ± 0.853
5.75AspGlu: 5.75 ± 1.44
4.237AspPhe: 4.237 ± 0.842
4.539AspGly: 4.539 ± 1.084
0.303AspHis: 0.303 ± 0.18
3.026AspIle: 3.026 ± 0.714
5.447AspLys: 5.447 ± 0.722
5.144AspLeu: 5.144 ± 0.824
3.177AspMet: 3.177 ± 0.793
3.48AspAsn: 3.48 ± 0.733
1.362AspPro: 1.362 ± 0.333
0.757AspGln: 0.757 ± 0.266
1.816AspArg: 1.816 ± 0.451
4.388AspSer: 4.388 ± 0.729
3.329AspThr: 3.329 ± 0.659
3.783AspVal: 3.783 ± 0.793
0.908AspTrp: 0.908 ± 0.506
2.572AspTyr: 2.572 ± 0.839
0.0AspXaa: 0.0 ± 0.0
Glu
3.329GluAla: 3.329 ± 0.57
0.757GluCys: 0.757 ± 0.324
3.783GluAsp: 3.783 ± 0.896
6.204GluGlu: 6.204 ± 1.513
4.388GluPhe: 4.388 ± 0.957
3.177GluGly: 3.177 ± 0.617
1.816GluHis: 1.816 ± 0.661
4.842GluIle: 4.842 ± 0.733
4.539GluLys: 4.539 ± 1.105
8.019GluLeu: 8.019 ± 0.987
3.783GluMet: 3.783 ± 0.809
3.631GluAsn: 3.631 ± 0.663
1.21GluPro: 1.21 ± 0.464
3.934GluGln: 3.934 ± 0.755
4.085GluArg: 4.085 ± 0.959
3.026GluSer: 3.026 ± 0.593
4.842GluThr: 4.842 ± 0.816
5.144GluVal: 5.144 ± 1.13
0.908GluTrp: 0.908 ± 0.354
2.572GluTyr: 2.572 ± 0.716
0.0GluXaa: 0.0 ± 0.0
Phe
2.421PheAla: 2.421 ± 0.576
0.757PheCys: 0.757 ± 0.297
2.724PheAsp: 2.724 ± 0.439
2.572PheGlu: 2.572 ± 0.647
1.513PhePhe: 1.513 ± 0.399
3.329PheGly: 3.329 ± 0.694
0.605PheHis: 0.605 ± 0.303
3.934PheIle: 3.934 ± 0.928
5.598PheLys: 5.598 ± 1.243
2.421PheLeu: 2.421 ± 0.515
1.21PheMet: 1.21 ± 0.408
3.631PheAsn: 3.631 ± 0.725
1.513PhePro: 1.513 ± 0.477
0.908PheGln: 0.908 ± 0.335
2.118PheArg: 2.118 ± 0.539
2.875PheSer: 2.875 ± 0.846
2.724PheThr: 2.724 ± 0.689
1.816PheVal: 1.816 ± 0.472
0.605PheTrp: 0.605 ± 0.378
1.664PheTyr: 1.664 ± 0.522
0.0PheXaa: 0.0 ± 0.0
Gly
4.388GlyAla: 4.388 ± 1.048
0.303GlyCys: 0.303 ± 0.225
4.237GlyAsp: 4.237 ± 0.868
2.572GlyGlu: 2.572 ± 0.626
3.934GlyPhe: 3.934 ± 0.969
5.144GlyGly: 5.144 ± 0.951
0.908GlyHis: 0.908 ± 0.307
4.539GlyIle: 4.539 ± 0.627
4.842GlyLys: 4.842 ± 0.8
5.901GlyLeu: 5.901 ± 0.751
1.967GlyMet: 1.967 ± 0.626
3.48GlyAsn: 3.48 ± 0.652
0.454GlyPro: 0.454 ± 0.219
2.875GlyGln: 2.875 ± 0.581
3.177GlyArg: 3.177 ± 0.722
4.993GlySer: 4.993 ± 1.183
4.691GlyThr: 4.691 ± 0.852
4.993GlyVal: 4.993 ± 1.044
1.513GlyTrp: 1.513 ± 0.516
4.085GlyTyr: 4.085 ± 0.84
0.0GlyXaa: 0.0 ± 0.0
His
0.908HisAla: 0.908 ± 0.401
0.151HisCys: 0.151 ± 0.144
0.908HisAsp: 0.908 ± 0.353
0.757HisGlu: 0.757 ± 0.402
1.059HisPhe: 1.059 ± 0.49
0.605HisGly: 0.605 ± 0.293
0.151HisHis: 0.151 ± 0.144
0.605HisIle: 0.605 ± 0.289
1.059HisLys: 1.059 ± 0.461
1.967HisLeu: 1.967 ± 0.531
0.303HisMet: 0.303 ± 0.201
0.605HisAsn: 0.605 ± 0.291
0.151HisPro: 0.151 ± 0.159
0.303HisGln: 0.303 ± 0.238
0.605HisArg: 0.605 ± 0.251
0.908HisSer: 0.908 ± 0.341
0.151HisThr: 0.151 ± 0.137
0.757HisVal: 0.757 ± 0.399
0.605HisTrp: 0.605 ± 0.274
1.21HisTyr: 1.21 ± 0.361
0.0HisXaa: 0.0 ± 0.0
Ile
3.783IleAla: 3.783 ± 0.709
0.151IleCys: 0.151 ± 0.159
4.993IleAsp: 4.993 ± 0.897
5.75IleGlu: 5.75 ± 1.08
1.513IlePhe: 1.513 ± 0.485
5.296IleGly: 5.296 ± 1.32
0.908IleHis: 0.908 ± 0.335
3.48IleIle: 3.48 ± 0.975
6.658IleLys: 6.658 ± 1.001
4.691IleLeu: 4.691 ± 0.731
1.362IleMet: 1.362 ± 0.388
4.842IleAsn: 4.842 ± 0.924
1.816IlePro: 1.816 ± 0.478
2.27IleGln: 2.27 ± 0.533
2.421IleArg: 2.421 ± 0.51
2.572IleSer: 2.572 ± 0.608
4.085IleThr: 4.085 ± 0.796
3.48IleVal: 3.48 ± 0.775
0.454IleTrp: 0.454 ± 0.277
2.27IleTyr: 2.27 ± 0.479
0.0IleXaa: 0.0 ± 0.0
Lys
8.625LysAla: 8.625 ± 1.209
0.454LysCys: 0.454 ± 0.24
6.204LysAsp: 6.204 ± 1.086
7.112LysGlu: 7.112 ± 1.192
3.177LysPhe: 3.177 ± 0.636
6.052LysGly: 6.052 ± 0.815
1.362LysHis: 1.362 ± 0.493
5.598LysIle: 5.598 ± 1.292
6.96LysLys: 6.96 ± 1.249
7.263LysLeu: 7.263 ± 1.27
3.026LysMet: 3.026 ± 0.612
3.783LysAsn: 3.783 ± 0.675
2.27LysPro: 2.27 ± 0.496
4.388LysGln: 4.388 ± 0.923
3.026LysArg: 3.026 ± 0.759
3.48LysSer: 3.48 ± 0.668
5.144LysThr: 5.144 ± 1.345
6.052LysVal: 6.052 ± 0.977
1.059LysTrp: 1.059 ± 0.543
3.783LysTyr: 3.783 ± 0.89
0.0LysXaa: 0.0 ± 0.0
Leu
4.539LeuAla: 4.539 ± 0.833
0.908LeuCys: 0.908 ± 0.322
4.085LeuAsp: 4.085 ± 0.741
7.565LeuGlu: 7.565 ± 1.235
4.085LeuPhe: 4.085 ± 0.61
6.658LeuGly: 6.658 ± 1.546
1.513LeuHis: 1.513 ± 0.581
5.447LeuIle: 5.447 ± 0.894
7.414LeuLys: 7.414 ± 1.341
6.052LeuLeu: 6.052 ± 1.152
3.026LeuMet: 3.026 ± 1.064
6.96LeuAsn: 6.96 ± 1.201
2.27LeuPro: 2.27 ± 0.525
3.329LeuGln: 3.329 ± 0.655
2.421LeuArg: 2.421 ± 0.628
5.598LeuSer: 5.598 ± 1.21
5.75LeuThr: 5.75 ± 0.975
4.842LeuVal: 4.842 ± 0.921
0.454LeuTrp: 0.454 ± 0.29
2.724LeuTyr: 2.724 ± 0.637
0.0LeuXaa: 0.0 ± 0.0
Met
3.783MetAla: 3.783 ± 0.655
0.0MetCys: 0.0 ± 0.0
1.816MetAsp: 1.816 ± 0.479
1.664MetGlu: 1.664 ± 0.705
1.21MetPhe: 1.21 ± 0.397
1.513MetGly: 1.513 ± 0.398
0.151MetHis: 0.151 ± 0.194
1.967MetIle: 1.967 ± 0.577
2.572MetLys: 2.572 ± 0.753
3.329MetLeu: 3.329 ± 0.865
0.151MetMet: 0.151 ± 0.16
2.118MetAsn: 2.118 ± 0.61
0.757MetPro: 0.757 ± 0.355
0.908MetGln: 0.908 ± 0.331
0.605MetArg: 0.605 ± 0.273
1.816MetSer: 1.816 ± 0.46
1.513MetThr: 1.513 ± 0.487
1.513MetVal: 1.513 ± 0.425
0.303MetTrp: 0.303 ± 0.21
1.059MetTyr: 1.059 ± 0.427
0.0MetXaa: 0.0 ± 0.0
Asn
5.296AsnAla: 5.296 ± 0.79
0.757AsnCys: 0.757 ± 0.369
3.631AsnAsp: 3.631 ± 0.922
5.296AsnGlu: 5.296 ± 1.04
2.724AsnPhe: 2.724 ± 0.541
5.447AsnGly: 5.447 ± 0.622
0.757AsnHis: 0.757 ± 0.387
3.329AsnIle: 3.329 ± 0.71
6.506AsnLys: 6.506 ± 1.015
4.388AsnLeu: 4.388 ± 0.639
1.513AsnMet: 1.513 ± 0.518
3.783AsnAsn: 3.783 ± 0.819
3.177AsnPro: 3.177 ± 0.915
1.664AsnGln: 1.664 ± 0.406
1.664AsnArg: 1.664 ± 0.554
3.026AsnSer: 3.026 ± 0.638
4.085AsnThr: 4.085 ± 1.02
3.329AsnVal: 3.329 ± 0.58
0.908AsnTrp: 0.908 ± 0.358
2.875AsnTyr: 2.875 ± 0.557
0.0AsnXaa: 0.0 ± 0.0
Pro
0.454ProAla: 0.454 ± 0.261
0.151ProCys: 0.151 ± 0.123
1.362ProAsp: 1.362 ± 0.534
1.513ProGlu: 1.513 ± 0.441
0.757ProPhe: 0.757 ± 0.423
0.0ProGly: 0.0 ± 0.0
0.151ProHis: 0.151 ± 0.175
1.362ProIle: 1.362 ± 0.481
2.875ProLys: 2.875 ± 0.676
3.026ProLeu: 3.026 ± 0.479
0.151ProMet: 0.151 ± 0.164
1.967ProAsn: 1.967 ± 0.69
1.059ProPro: 1.059 ± 0.439
1.664ProGln: 1.664 ± 0.579
0.605ProArg: 0.605 ± 0.32
2.118ProSer: 2.118 ± 0.606
1.513ProThr: 1.513 ± 0.523
1.664ProVal: 1.664 ± 0.532
0.454ProTrp: 0.454 ± 0.287
1.362ProTyr: 1.362 ± 0.532
0.0ProXaa: 0.0 ± 0.0
Gln
3.631GlnAla: 3.631 ± 1.186
0.151GlnCys: 0.151 ± 0.155
1.513GlnAsp: 1.513 ± 0.538
1.513GlnGlu: 1.513 ± 0.426
1.664GlnPhe: 1.664 ± 0.544
2.27GlnGly: 2.27 ± 0.452
0.303GlnHis: 0.303 ± 0.229
3.48GlnIle: 3.48 ± 0.684
3.48GlnLys: 3.48 ± 0.754
2.724GlnLeu: 2.724 ± 0.515
1.21GlnMet: 1.21 ± 0.522
2.875GlnAsn: 2.875 ± 0.831
0.303GlnPro: 0.303 ± 0.197
1.664GlnGln: 1.664 ± 0.569
1.967GlnArg: 1.967 ± 0.613
2.724GlnSer: 2.724 ± 0.644
1.816GlnThr: 1.816 ± 0.452
2.421GlnVal: 2.421 ± 0.571
0.605GlnTrp: 0.605 ± 0.293
1.967GlnTyr: 1.967 ± 0.636
0.0GlnXaa: 0.0 ± 0.0
Arg
2.118ArgAla: 2.118 ± 0.63
0.151ArgCys: 0.151 ± 0.145
1.362ArgAsp: 1.362 ± 0.411
4.842ArgGlu: 4.842 ± 1.238
1.816ArgPhe: 1.816 ± 0.526
2.118ArgGly: 2.118 ± 0.567
0.757ArgHis: 0.757 ± 0.268
1.362ArgIle: 1.362 ± 0.387
4.237ArgLys: 4.237 ± 0.713
2.572ArgLeu: 2.572 ± 0.579
1.513ArgMet: 1.513 ± 0.461
1.816ArgAsn: 1.816 ± 0.662
1.059ArgPro: 1.059 ± 0.611
1.059ArgGln: 1.059 ± 0.328
1.059ArgArg: 1.059 ± 0.58
2.572ArgSer: 2.572 ± 0.67
1.664ArgThr: 1.664 ± 0.543
2.27ArgVal: 2.27 ± 0.733
0.303ArgTrp: 0.303 ± 0.244
1.664ArgTyr: 1.664 ± 0.501
0.0ArgXaa: 0.0 ± 0.0
Ser
3.026SerAla: 3.026 ± 1.048
0.0SerCys: 0.0 ± 0.0
3.329SerAsp: 3.329 ± 0.647
4.539SerGlu: 4.539 ± 0.944
2.118SerPhe: 2.118 ± 0.504
4.993SerGly: 4.993 ± 0.939
0.908SerHis: 0.908 ± 0.302
4.085SerIle: 4.085 ± 0.789
4.691SerLys: 4.691 ± 0.837
5.75SerLeu: 5.75 ± 0.907
1.059SerMet: 1.059 ± 0.364
3.48SerAsn: 3.48 ± 0.677
0.757SerPro: 0.757 ± 0.307
2.572SerGln: 2.572 ± 0.809
1.816SerArg: 1.816 ± 0.423
3.026SerSer: 3.026 ± 0.948
3.177SerThr: 3.177 ± 0.98
4.842SerVal: 4.842 ± 0.829
0.757SerTrp: 0.757 ± 0.339
3.329SerTyr: 3.329 ± 0.803
0.0SerXaa: 0.0 ± 0.0
Thr
3.329ThrAla: 3.329 ± 0.597
0.454ThrCys: 0.454 ± 0.338
4.237ThrAsp: 4.237 ± 0.892
3.783ThrGlu: 3.783 ± 0.904
2.724ThrPhe: 2.724 ± 0.664
6.204ThrGly: 6.204 ± 1.111
0.908ThrHis: 0.908 ± 0.387
4.993ThrIle: 4.993 ± 0.634
5.598ThrLys: 5.598 ± 0.995
5.75ThrLeu: 5.75 ± 1.22
0.605ThrMet: 0.605 ± 0.443
3.177ThrAsn: 3.177 ± 0.816
2.27ThrPro: 2.27 ± 0.569
2.572ThrGln: 2.572 ± 0.701
1.664ThrArg: 1.664 ± 0.396
3.177ThrSer: 3.177 ± 0.781
3.329ThrThr: 3.329 ± 0.68
4.085ThrVal: 4.085 ± 0.897
1.21ThrTrp: 1.21 ± 0.446
2.118ThrTyr: 2.118 ± 0.569
0.0ThrXaa: 0.0 ± 0.0
Val
3.329ValAla: 3.329 ± 0.524
0.303ValCys: 0.303 ± 0.237
4.842ValAsp: 4.842 ± 0.91
4.237ValGlu: 4.237 ± 0.976
2.27ValPhe: 2.27 ± 0.589
2.875ValGly: 2.875 ± 0.752
0.757ValHis: 0.757 ± 0.327
2.875ValIle: 2.875 ± 0.755
5.447ValLys: 5.447 ± 0.863
4.539ValLeu: 4.539 ± 0.899
1.816ValMet: 1.816 ± 0.507
4.539ValAsn: 4.539 ± 0.883
1.816ValPro: 1.816 ± 0.547
1.967ValGln: 1.967 ± 0.651
3.329ValArg: 3.329 ± 0.726
4.237ValSer: 4.237 ± 0.986
4.237ValThr: 4.237 ± 0.754
2.875ValVal: 2.875 ± 0.751
1.513ValTrp: 1.513 ± 0.455
3.177ValTyr: 3.177 ± 0.726
0.0ValXaa: 0.0 ± 0.0
Trp
1.362TrpAla: 1.362 ± 0.433
0.454TrpCys: 0.454 ± 0.217
0.605TrpAsp: 0.605 ± 0.295
0.908TrpGlu: 0.908 ± 0.333
0.908TrpPhe: 0.908 ± 0.302
1.664TrpGly: 1.664 ± 0.457
0.303TrpHis: 0.303 ± 0.187
0.303TrpIle: 0.303 ± 0.189
0.605TrpLys: 0.605 ± 0.259
2.27TrpLeu: 2.27 ± 0.767
0.303TrpMet: 0.303 ± 0.166
1.21TrpAsn: 1.21 ± 0.617
0.0TrpPro: 0.0 ± 0.0
0.757TrpGln: 0.757 ± 0.326
0.303TrpArg: 0.303 ± 0.219
0.151TrpSer: 0.151 ± 0.145
1.21TrpThr: 1.21 ± 0.48
0.454TrpVal: 0.454 ± 0.356
0.303TrpTrp: 0.303 ± 0.19
0.454TrpTyr: 0.454 ± 0.232
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.48TyrAla: 3.48 ± 0.613
0.605TyrCys: 0.605 ± 0.288
3.026TyrAsp: 3.026 ± 0.682
2.118TyrGlu: 2.118 ± 0.563
2.421TyrPhe: 2.421 ± 0.57
3.48TyrGly: 3.48 ± 1.027
1.059TyrHis: 1.059 ± 0.424
3.026TyrIle: 3.026 ± 0.611
3.934TyrLys: 3.934 ± 0.646
1.664TyrLeu: 1.664 ± 0.498
0.605TyrMet: 0.605 ± 0.375
3.934TyrAsn: 3.934 ± 0.669
1.059TyrPro: 1.059 ± 0.357
1.513TyrGln: 1.513 ± 0.424
1.21TyrArg: 1.21 ± 0.455
2.421TyrSer: 2.421 ± 0.636
3.934TyrThr: 3.934 ± 0.648
2.27TyrVal: 2.27 ± 0.648
0.908TyrTrp: 0.908 ± 0.348
2.724TyrTyr: 2.724 ± 0.714
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 37 proteins (6610 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski