Grapevine leafroll-associated virus 10

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus; unclassified Ampelovirus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7FAV2|B7FAV2_9CLOS Leader protease OS=Grapevine leafroll-associated virus 10 OX=367121 PE=4 SV=1
MM1 pKa = 7.1EE2 pKa = 4.45TVICLSKK9 pKa = 10.72VKK11 pKa = 10.64HH12 pKa = 5.45EE13 pKa = 4.33VPVPEE18 pKa = 4.03EE19 pKa = 3.55QVFFLVNSGVFAGVLPSEE37 pKa = 4.5HH38 pKa = 7.1LSFFYY43 pKa = 10.94DD44 pKa = 3.99KK45 pKa = 11.5LNTGDD50 pKa = 3.47NKK52 pKa = 10.67DD53 pKa = 3.22RR54 pKa = 11.84RR55 pKa = 11.84TYY57 pKa = 10.46PEE59 pKa = 4.15LFKK62 pKa = 11.21FLDD65 pKa = 3.39NLGNVVATNNGNFMKK80 pKa = 10.53FLDD83 pKa = 3.63AVTIKK88 pKa = 10.54EE89 pKa = 4.24YY90 pKa = 10.59FKK92 pKa = 10.66RR93 pKa = 11.84RR94 pKa = 11.84YY95 pKa = 8.9PSTVHH100 pKa = 6.52IDD102 pKa = 3.27SSSKK106 pKa = 10.64VSGGDD111 pKa = 3.53YY112 pKa = 11.07CCLTVPALATFLSGVAHH129 pKa = 6.52HH130 pKa = 7.08LEE132 pKa = 4.29GLTKK136 pKa = 10.12VLAVGFTRR144 pKa = 11.84SCDD147 pKa = 3.94SEE149 pKa = 4.38EE150 pKa = 3.6LWAFKK155 pKa = 10.87DD156 pKa = 3.57SFVVFEE162 pKa = 4.47NAGKK166 pKa = 10.59LDD168 pKa = 3.94VFCGDD173 pKa = 4.17NMNTSNSAVLEE184 pKa = 4.26VFNSFRR190 pKa = 11.84AASSATDD197 pKa = 3.28MEE199 pKa = 4.41RR200 pKa = 11.84VVNLLSII207 pKa = 4.48

Molecular weight: 22.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7FAV4|B7FAV4_9CLOS Small hydrophobic protein OS=Grapevine leafroll-associated virus 10 OX=367121 GN=p5 PE=4 SV=1
MM1 pKa = 7.91LDD3 pKa = 4.96LFAQFNWVFQIAAFVLIILFFAVLALVIQRR33 pKa = 11.84VFYY36 pKa = 11.0NSVRR40 pKa = 11.84GPAPPSS46 pKa = 3.24

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4407

46

2294

629.6

69.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.354 ± 0.427

1.997 ± 0.26

5.31 ± 0.484

5.491 ± 0.136

5.31 ± 0.728

6.331 ± 0.316

1.725 ± 0.241

4.516 ± 0.363

6.558 ± 0.414

9.712 ± 0.578

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.747 ± 0.186

4.516 ± 0.475

4.742 ± 0.499

2.564 ± 0.296

5.287 ± 0.271

9.916 ± 0.877

5.31 ± 0.22

9.008 ± 0.637

0.771 ± 0.117

2.836 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski