Amino acid dipepetide frequency for Paenibacillus whitsoniae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.931AlaAla: 9.931 ± 0.109	0.766AlaCys: 0.766 ± 0.023	4.496AlaAsp: 4.496 ± 0.051	5.463AlaGlu: 5.463 ± 0.062	3.518AlaPhe: 3.518 ± 0.045	7.057AlaGly: 7.057 ± 0.067	1.564AlaHis: 1.564 ± 0.029	5.573AlaIle: 5.573 ± 0.056	4.528AlaLys: 4.528 ± 0.051	8.596AlaLeu: 8.596 ± 0.079	2.446AlaMet: 2.446 ± 0.04	3.095AlaAsn: 3.095 ± 0.045	3.271AlaPro: 3.271 ± 0.052	3.28AlaGln: 3.28 ± 0.046	3.555AlaArg: 3.555 ± 0.044	5.938AlaSer: 5.938 ± 0.063	4.369AlaThr: 4.369 ± 0.056	6.685AlaVal: 6.685 ± 0.06	1.115AlaTrp: 1.115 ± 0.023	3.15AlaTyr: 3.15 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.54CysAla: 0.54 ± 0.016	0.094CysCys: 0.094 ± 0.007	0.342CysAsp: 0.342 ± 0.014	0.418CysGlu: 0.418 ± 0.013	0.283CysPhe: 0.283 ± 0.013	0.68CysGly: 0.68 ± 0.019	0.173CysHis: 0.173 ± 0.009	0.441CysIle: 0.441 ± 0.015	0.289CysLys: 0.289 ± 0.013	0.741CysLeu: 0.741 ± 0.02	0.199CysMet: 0.199 ± 0.009	0.223CysAsn: 0.223 ± 0.011	0.314CysPro: 0.314 ± 0.014	0.234CysGln: 0.234 ± 0.01	0.402CysArg: 0.402 ± 0.014	0.537CysSer: 0.537 ± 0.016	0.402CysThr: 0.402 ± 0.015	0.464CysVal: 0.464 ± 0.016	0.086CysTrp: 0.086 ± 0.006	0.254CysTyr: 0.254 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	4.406AspAla: 4.406 ± 0.043	0.35AspCys: 0.35 ± 0.013	2.376AspAsp: 2.376 ± 0.037	3.351AspGlu: 3.351 ± 0.039	2.223AspPhe: 2.223 ± 0.035	4.231AspGly: 4.231 ± 0.059	1.09AspHis: 1.09 ± 0.027	3.247AspIle: 3.247 ± 0.047	2.599AspLys: 2.599 ± 0.043	4.796AspLeu: 4.796 ± 0.047	1.322AspMet: 1.322 ± 0.022	1.901AspAsn: 1.901 ± 0.036	2.361AspPro: 2.361 ± 0.036	1.838AspGln: 1.838 ± 0.032	2.619AspArg: 2.619 ± 0.035	2.741AspSer: 2.741 ± 0.04	2.648AspThr: 2.648 ± 0.039	3.746AspVal: 3.746 ± 0.049	0.931AspTrp: 0.931 ± 0.02	2.082AspTyr: 2.082 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	6.145GluAla: 6.145 ± 0.072	0.305GluCys: 0.305 ± 0.012	2.798GluAsp: 2.798 ± 0.045	4.504GluGlu: 4.504 ± 0.065	1.984GluPhe: 1.984 ± 0.034	4.146GluGly: 4.146 ± 0.048	1.418GluHis: 1.418 ± 0.031	3.764GluIle: 3.764 ± 0.049	3.522GluLys: 3.522 ± 0.051	6.71GluLeu: 6.71 ± 0.073	1.825GluMet: 1.825 ± 0.031	2.144GluAsn: 2.144 ± 0.03	2.185GluPro: 2.185 ± 0.032	3.278GluGln: 3.278 ± 0.045	3.599GluArg: 3.599 ± 0.056	3.134GluSer: 3.134 ± 0.037	3.2GluThr: 3.2 ± 0.04	4.116GluVal: 4.116 ± 0.051	0.802GluTrp: 0.802 ± 0.019	1.666GluTyr: 1.666 ± 0.028	0.001GluXaa: 0.001 ± 0.001
Phe	3.335PheAla: 3.335 ± 0.045	0.315PheCys: 0.315 ± 0.011	2.264PheAsp: 2.264 ± 0.031	2.323PheGlu: 2.323 ± 0.037	1.839PhePhe: 1.839 ± 0.039	3.141PheGly: 3.141 ± 0.04	0.943PheHis: 0.943 ± 0.021	2.777PheIle: 2.777 ± 0.042	2.05PheLys: 2.05 ± 0.034	3.877PheLeu: 3.877 ± 0.053	1.15PheMet: 1.15 ± 0.025	1.629PheAsn: 1.629 ± 0.03	1.662PhePro: 1.662 ± 0.028	1.637PheGln: 1.637 ± 0.024	1.961PheArg: 1.961 ± 0.033	2.814PheSer: 2.814 ± 0.036	2.649PheThr: 2.649 ± 0.043	2.941PheVal: 2.941 ± 0.036	0.561PheTrp: 0.561 ± 0.016	1.546PheTyr: 1.546 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	6.059GlyAla: 6.059 ± 0.065	0.685GlyCys: 0.685 ± 0.02	3.61GlyAsp: 3.61 ± 0.045	4.261GlyGlu: 4.261 ± 0.059	3.235GlyPhe: 3.235 ± 0.042	5.534GlyGly: 5.534 ± 0.077	1.499GlyHis: 1.499 ± 0.026	5.373GlyIle: 5.373 ± 0.056	4.402GlyLys: 4.402 ± 0.053	7.262GlyLeu: 7.262 ± 0.061	2.206GlyMet: 2.206 ± 0.03	2.813GlyAsn: 2.813 ± 0.05	2.012GlyPro: 2.012 ± 0.038	2.648GlyGln: 2.648 ± 0.039	3.33GlyArg: 3.33 ± 0.047	4.969GlySer: 4.969 ± 0.057	4.885GlyThr: 4.885 ± 0.064	5.4GlyVal: 5.4 ± 0.055	1.195GlyTrp: 1.195 ± 0.025	2.89GlyTyr: 2.89 ± 0.041	0.001GlyXaa: 0.001 ± 0.001
His	1.768HisAla: 1.768 ± 0.031	0.173HisCys: 0.173 ± 0.01	1.006HisAsp: 1.006 ± 0.025	1.212HisGlu: 1.212 ± 0.023	1.117HisPhe: 1.117 ± 0.023	1.539HisGly: 1.539 ± 0.031	0.644HisHis: 0.644 ± 0.025	1.385HisIle: 1.385 ± 0.027	0.814HisLys: 0.814 ± 0.022	2.109HisLeu: 2.109 ± 0.037	0.581HisMet: 0.581 ± 0.016	0.706HisAsn: 0.706 ± 0.019	1.166HisPro: 1.166 ± 0.024	0.755HisGln: 0.755 ± 0.02	1.072HisArg: 1.072 ± 0.024	1.072HisSer: 1.072 ± 0.026	1.117HisThr: 1.117 ± 0.024	1.571HisVal: 1.571 ± 0.026	0.342HisTrp: 0.342 ± 0.014	0.927HisTyr: 0.927 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.933IleAla: 5.933 ± 0.061	0.528IleCys: 0.528 ± 0.017	3.497IleAsp: 3.497 ± 0.039	3.839IleGlu: 3.839 ± 0.047	2.315IlePhe: 2.315 ± 0.038	5.13IleGly: 5.13 ± 0.054	1.407IleHis: 1.407 ± 0.028	3.702IleIle: 3.702 ± 0.053	2.729IleLys: 2.729 ± 0.041	5.513IleLeu: 5.513 ± 0.063	1.54IleMet: 1.54 ± 0.027	2.314IleAsn: 2.314 ± 0.034	3.08IlePro: 3.08 ± 0.039	2.558IleGln: 2.558 ± 0.037	3.609IleArg: 3.609 ± 0.047	4.338IleSer: 4.338 ± 0.053	3.812IleThr: 3.812 ± 0.049	5.114IleVal: 5.114 ± 0.057	0.762IleTrp: 0.762 ± 0.02	2.046IleTyr: 2.046 ± 0.028	0.0IleXaa: 0.0 ± 0.001
Lys	4.512LysAla: 4.512 ± 0.048	0.21LysCys: 0.21 ± 0.01	2.912LysAsp: 2.912 ± 0.042	3.772LysGlu: 3.772 ± 0.049	1.595LysPhe: 1.595 ± 0.033	3.535LysGly: 3.535 ± 0.043	1.039LysHis: 1.039 ± 0.02	2.933LysIle: 2.933 ± 0.043	3.141LysLys: 3.141 ± 0.054	5.428LysLeu: 5.428 ± 0.065	1.587LysMet: 1.587 ± 0.029	2.013LysAsn: 2.013 ± 0.039	2.332LysPro: 2.332 ± 0.032	2.486LysGln: 2.486 ± 0.037	2.525LysArg: 2.525 ± 0.041	2.887LysSer: 2.887 ± 0.04	2.726LysThr: 2.726 ± 0.042	3.562LysVal: 3.562 ± 0.052	0.735LysTrp: 0.735 ± 0.018	1.775LysTyr: 1.775 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	8.79LeuAla: 8.79 ± 0.085	0.772LeuCys: 0.772 ± 0.019	5.042LeuAsp: 5.042 ± 0.055	5.691LeuGlu: 5.691 ± 0.067	4.465LeuPhe: 4.465 ± 0.054	6.627LeuGly: 6.627 ± 0.058	2.225LeuHis: 2.225 ± 0.037	6.273LeuIle: 6.273 ± 0.065	5.012LeuLys: 5.012 ± 0.049	10.798LeuLeu: 10.798 ± 0.098	2.579LeuMet: 2.579 ± 0.036	3.806LeuAsn: 3.806 ± 0.048	4.71LeuPro: 4.71 ± 0.048	4.521LeuGln: 4.521 ± 0.052	5.063LeuArg: 5.063 ± 0.059	6.817LeuSer: 6.817 ± 0.072	6.157LeuThr: 6.157 ± 0.06	6.517LeuVal: 6.517 ± 0.055	1.099LeuTrp: 1.099 ± 0.027	3.268LeuTyr: 3.268 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.308MetAla: 2.308 ± 0.036	0.153MetCys: 0.153 ± 0.008	1.416MetAsp: 1.416 ± 0.028	1.661MetGlu: 1.661 ± 0.033	1.071MetPhe: 1.071 ± 0.023	1.678MetGly: 1.678 ± 0.031	0.498MetHis: 0.498 ± 0.017	1.823MetIle: 1.823 ± 0.032	1.986MetLys: 1.986 ± 0.033	3.024MetLeu: 3.024 ± 0.041	0.92MetMet: 0.92 ± 0.023	1.421MetAsn: 1.421 ± 0.029	1.231MetPro: 1.231 ± 0.024	1.073MetGln: 1.073 ± 0.02	1.331MetArg: 1.331 ± 0.026	1.79MetSer: 1.79 ± 0.031	1.718MetThr: 1.718 ± 0.031	1.713MetVal: 1.713 ± 0.026	0.267MetTrp: 0.267 ± 0.01	0.825MetTyr: 0.825 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.074AsnAla: 3.074 ± 0.041	0.232AsnCys: 0.232 ± 0.01	1.848AsnAsp: 1.848 ± 0.034	2.299AsnGlu: 2.299 ± 0.032	1.475AsnPhe: 1.475 ± 0.028	3.378AsnGly: 3.378 ± 0.055	0.829AsnHis: 0.829 ± 0.021	2.193AsnIle: 2.193 ± 0.033	1.967AsnLys: 1.967 ± 0.039	3.39AsnLeu: 3.39 ± 0.046	1.021AsnMet: 1.021 ± 0.021	1.652AsnAsn: 1.652 ± 0.037	2.047AsnPro: 2.047 ± 0.034	1.528AsnGln: 1.528 ± 0.03	1.951AsnArg: 1.951 ± 0.032	2.178AsnSer: 2.178 ± 0.037	2.234AsnThr: 2.234 ± 0.045	2.914AsnVal: 2.914 ± 0.045	0.631AsnTrp: 0.631 ± 0.021	1.597AsnTyr: 1.597 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	3.801ProAla: 3.801 ± 0.052	0.241ProCys: 0.241 ± 0.011	2.583ProAsp: 2.583 ± 0.035	2.974ProGlu: 2.974 ± 0.041	1.898ProPhe: 1.898 ± 0.033	3.096ProGly: 3.096 ± 0.039	0.96ProHis: 0.96 ± 0.022	2.672ProIle: 2.672 ± 0.036	1.806ProLys: 1.806 ± 0.029	4.064ProLeu: 4.064 ± 0.046	1.059ProMet: 1.059 ± 0.022	1.713ProAsn: 1.713 ± 0.034	1.403ProPro: 1.403 ± 0.03	1.53ProGln: 1.53 ± 0.027	1.375ProArg: 1.375 ± 0.028	2.755ProSer: 2.755 ± 0.04	2.299ProThr: 2.299 ± 0.037	3.285ProVal: 3.285 ± 0.04	0.571ProTrp: 0.571 ± 0.018	1.618ProTyr: 1.618 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.971GlnAla: 3.971 ± 0.052	0.203GlnCys: 0.203 ± 0.01	1.888GlnAsp: 1.888 ± 0.028	2.585GlnGlu: 2.585 ± 0.037	1.631GlnPhe: 1.631 ± 0.026	2.803GlnGly: 2.803 ± 0.042	0.887GlnHis: 0.887 ± 0.021	2.502GlnIle: 2.502 ± 0.032	1.882GlnLys: 1.882 ± 0.03	4.521GlnLeu: 4.521 ± 0.055	1.206GlnMet: 1.206 ± 0.022	1.285GlnAsn: 1.285 ± 0.029	1.706GlnPro: 1.706 ± 0.034	1.931GlnGln: 1.931 ± 0.039	1.877GlnArg: 1.877 ± 0.036	2.334GlnSer: 2.334 ± 0.035	2.315GlnThr: 2.315 ± 0.034	2.798GlnVal: 2.798 ± 0.037	0.555GlnTrp: 0.555 ± 0.017	1.315GlnTyr: 1.315 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	3.483ArgAla: 3.483 ± 0.044	0.345ArgCys: 0.345 ± 0.013	2.282ArgAsp: 2.282 ± 0.035	3.414ArgGlu: 3.414 ± 0.05	2.139ArgPhe: 2.139 ± 0.029	2.94ArgGly: 2.94 ± 0.037	1.027ArgHis: 1.027 ± 0.022	3.554ArgIle: 3.554 ± 0.044	2.82ArgLys: 2.82 ± 0.038	5.026ArgLeu: 5.026 ± 0.057	1.629ArgMet: 1.629 ± 0.028	1.901ArgAsn: 1.901 ± 0.028	1.602ArgPro: 1.602 ± 0.029	1.974ArgGln: 1.974 ± 0.033	2.454ArgArg: 2.454 ± 0.04	3.0ArgSer: 3.0 ± 0.04	2.72ArgThr: 2.72 ± 0.036	3.189ArgVal: 3.189 ± 0.043	0.698ArgTrp: 0.698 ± 0.018	1.815ArgTyr: 1.815 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	5.335SerAla: 5.335 ± 0.059	0.426SerCys: 0.426 ± 0.014	3.104SerAsp: 3.104 ± 0.046	3.544SerGlu: 3.544 ± 0.042	2.948SerPhe: 2.948 ± 0.04	5.531SerGly: 5.531 ± 0.062	1.21SerHis: 1.21 ± 0.02	4.032SerIle: 4.032 ± 0.05	3.069SerLys: 3.069 ± 0.043	6.396SerLeu: 6.396 ± 0.065	1.779SerMet: 1.779 ± 0.027	2.334SerAsn: 2.334 ± 0.038	2.619SerPro: 2.619 ± 0.038	2.198SerGln: 2.198 ± 0.036	2.859SerArg: 2.859 ± 0.041	4.552SerSer: 4.552 ± 0.066	3.629SerThr: 3.629 ± 0.06	4.567SerVal: 4.567 ± 0.062	0.909SerTrp: 0.909 ± 0.021	2.423SerTyr: 2.423 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	5.324ThrAla: 5.324 ± 0.063	0.342ThrCys: 0.342 ± 0.014	3.087ThrAsp: 3.087 ± 0.046	3.027ThrGlu: 3.027 ± 0.04	2.57ThrPhe: 2.57 ± 0.039	4.762ThrGly: 4.762 ± 0.06	1.037ThrHis: 1.037 ± 0.024	3.884ThrIle: 3.884 ± 0.044	2.63ThrLys: 2.63 ± 0.038	5.869ThrLeu: 5.869 ± 0.052	1.469ThrMet: 1.469 ± 0.028	2.275ThrAsn: 2.275 ± 0.046	2.821ThrPro: 2.821 ± 0.041	1.846ThrGln: 1.846 ± 0.035	2.217ThrArg: 2.217 ± 0.031	3.708ThrSer: 3.708 ± 0.061	3.496ThrThr: 3.496 ± 0.067	4.792ThrVal: 4.792 ± 0.057	0.821ThrTrp: 0.821 ± 0.025	2.334ThrTyr: 2.334 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	5.658ValAla: 5.658 ± 0.059	0.601ValCys: 0.601 ± 0.016	3.71ValAsp: 3.71 ± 0.043	4.066ValGlu: 4.066 ± 0.047	2.877ValPhe: 2.877 ± 0.044	4.797ValGly: 4.797 ± 0.048	1.503ValHis: 1.503 ± 0.031	4.753ValIle: 4.753 ± 0.046	3.988ValLys: 3.988 ± 0.049	7.101ValLeu: 7.101 ± 0.066	1.963ValMet: 1.963 ± 0.027	2.973ValAsn: 2.973 ± 0.043	3.175ValPro: 3.175 ± 0.042	2.93ValGln: 2.93 ± 0.038	3.479ValArg: 3.479 ± 0.04	4.95ValSer: 4.95 ± 0.054	4.94ValThr: 4.94 ± 0.076	5.152ValVal: 5.152 ± 0.053	0.935ValTrp: 0.935 ± 0.023	2.58ValTyr: 2.58 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.962TrpAla: 0.962 ± 0.022	0.098TrpCys: 0.098 ± 0.007	0.729TrpAsp: 0.729 ± 0.021	0.747TrpGlu: 0.747 ± 0.019	0.579TrpPhe: 0.579 ± 0.016	0.935TrpGly: 0.935 ± 0.022	0.339TrpHis: 0.339 ± 0.013	0.859TrpIle: 0.859 ± 0.021	0.785TrpLys: 0.785 ± 0.022	1.509TrpLeu: 1.509 ± 0.026	0.464TrpMet: 0.464 ± 0.016	0.714TrpAsn: 0.714 ± 0.019	0.426TrpPro: 0.426 ± 0.014	0.624TrpGln: 0.624 ± 0.016	0.669TrpArg: 0.669 ± 0.018	0.866TrpSer: 0.866 ± 0.023	0.819TrpThr: 0.819 ± 0.025	0.935TrpVal: 0.935 ± 0.019	0.214TrpTrp: 0.214 ± 0.011	0.474TrpTyr: 0.474 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.06TyrAla: 3.06 ± 0.042	0.276TyrCys: 0.276 ± 0.012	1.871TyrAsp: 1.871 ± 0.036	2.096TyrGlu: 2.096 ± 0.03	1.651TyrPhe: 1.651 ± 0.028	2.728TyrGly: 2.728 ± 0.032	0.785TyrHis: 0.785 ± 0.021	2.026TyrIle: 2.026 ± 0.035	1.684TyrLys: 1.684 ± 0.028	3.502TyrLeu: 3.502 ± 0.041	0.96TyrMet: 0.96 ± 0.021	1.527TyrAsn: 1.527 ± 0.034	1.583TyrPro: 1.583 ± 0.029	1.327TyrGln: 1.327 ± 0.028	2.064TyrArg: 2.064 ± 0.036	2.085TyrSer: 2.085 ± 0.035	2.177TyrThr: 2.177 ± 0.042	2.669TyrVal: 2.669 ± 0.04	0.513TyrTrp: 0.513 ± 0.016	1.428TyrTyr: 1.428 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 6143 proteins (2161665 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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