Sewage-associated circular DNA virus-11

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075IZM2|A0A075IZM2_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-11 OX=1519387 PE=3 SV=1
MM1 pKa = 7.39ATTTSSEE8 pKa = 3.91QSNKK12 pKa = 9.56SNRR15 pKa = 11.84TKK17 pKa = 10.5RR18 pKa = 11.84LSRR21 pKa = 11.84FVFTLNNYY29 pKa = 6.85TAEE32 pKa = 4.14EE33 pKa = 4.05EE34 pKa = 4.55QVIQLLGTTLKK45 pKa = 10.07MKK47 pKa = 9.33WLIYY51 pKa = 9.76GRR53 pKa = 11.84EE54 pKa = 4.13VGEE57 pKa = 4.45KK58 pKa = 8.96GTPHH62 pKa = 6.2LQGAAVIGRR71 pKa = 11.84QLAWKK76 pKa = 7.96TIKK79 pKa = 10.66NSFPPRR85 pKa = 11.84THH87 pKa = 7.62LEE89 pKa = 4.1EE90 pKa = 4.2MKK92 pKa = 9.84GTPRR96 pKa = 11.84EE97 pKa = 4.04SFIYY101 pKa = 8.64CTKK104 pKa = 10.33EE105 pKa = 3.56DD106 pKa = 3.3KK107 pKa = 10.46EE108 pKa = 4.4YY109 pKa = 10.5YY110 pKa = 10.05QFGEE114 pKa = 4.1EE115 pKa = 4.14PKK117 pKa = 10.03PGKK120 pKa = 10.63RR121 pKa = 11.84NDD123 pKa = 3.92LLDD126 pKa = 3.85AVASLRR132 pKa = 11.84KK133 pKa = 8.09TSSFRR138 pKa = 11.84DD139 pKa = 4.03FVKK142 pKa = 10.52DD143 pKa = 3.56DD144 pKa = 3.7QNASVYY150 pKa = 10.4VKK152 pKa = 7.98YY153 pKa = 9.42TRR155 pKa = 11.84GLTAFRR161 pKa = 11.84SLLRR165 pKa = 11.84EE166 pKa = 3.88PRR168 pKa = 11.84SPSRR172 pKa = 11.84PPTVYY177 pKa = 9.6WLHH180 pKa = 6.46GPTGAGKK187 pKa = 8.08TKK189 pKa = 10.3HH190 pKa = 5.74AWEE193 pKa = 4.07FLEE196 pKa = 6.21GYY198 pKa = 10.15FGTDD202 pKa = 2.83QEE204 pKa = 4.68TWISNGSLRR213 pKa = 11.84WFDD216 pKa = 4.27GYY218 pKa = 11.02DD219 pKa = 3.24GHH221 pKa = 7.99RR222 pKa = 11.84GVILDD227 pKa = 5.12DD228 pKa = 3.37IRR230 pKa = 11.84ADD232 pKa = 3.41SVPYY236 pKa = 10.76NEE238 pKa = 5.5LLRR241 pKa = 11.84LLDD244 pKa = 4.16RR245 pKa = 11.84YY246 pKa = 9.74PYY248 pKa = 9.57RR249 pKa = 11.84VEE251 pKa = 4.14YY252 pKa = 10.65KK253 pKa = 10.64GGTTEE258 pKa = 5.05WIPEE262 pKa = 4.2IIIITAPMGPYY273 pKa = 9.85QFFRR277 pKa = 11.84VGDD280 pKa = 4.52DD281 pKa = 3.01IEE283 pKa = 4.21QLLRR287 pKa = 11.84RR288 pKa = 11.84VNGGIFEE295 pKa = 3.99FTIDD299 pKa = 3.77GPNPRR304 pKa = 11.84LQTVAPTVPNTPAHH318 pKa = 6.35SEE320 pKa = 4.18VEE322 pKa = 4.38SQDD325 pKa = 4.01DD326 pKa = 3.82VQEE329 pKa = 4.19LQEE332 pKa = 4.25SSEE335 pKa = 4.08GLSLLEE341 pKa = 4.29SIGRR345 pKa = 11.84GSGIEE350 pKa = 3.8QDD352 pKa = 3.29QSNSQEE358 pKa = 4.19EE359 pKa = 4.34EE360 pKa = 3.78TQLRR364 pKa = 11.84KK365 pKa = 9.55KK366 pKa = 10.63RR367 pKa = 11.84KK368 pKa = 9.75FIDD371 pKa = 3.07

Molecular weight: 42.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075IZM2|A0A075IZM2_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-11 OX=1519387 PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 9.74RR3 pKa = 11.84QRR5 pKa = 11.84SAYY8 pKa = 9.16EE9 pKa = 3.41IEE11 pKa = 4.07NRR13 pKa = 11.84KK14 pKa = 8.02QAARR18 pKa = 11.84AKK20 pKa = 10.09RR21 pKa = 11.84RR22 pKa = 11.84ALVAARR28 pKa = 11.84AAPMRR33 pKa = 11.84AYY35 pKa = 10.48ANSQVPLRR43 pKa = 11.84SGGYY47 pKa = 9.75RR48 pKa = 11.84FGSTEE53 pKa = 3.83KK54 pKa = 10.74KK55 pKa = 10.8VFDD58 pKa = 5.26LDD60 pKa = 3.77TGTTNINTTGSVLALFVPTLGTDD83 pKa = 2.99MTNRR87 pKa = 11.84IGRR90 pKa = 11.84KK91 pKa = 9.36AIIKK95 pKa = 9.64SFYY98 pKa = 8.99LRR100 pKa = 11.84GYY102 pKa = 7.98VRR104 pKa = 11.84CEE106 pKa = 3.57NSLTPTSPQAGSSQQLRR123 pKa = 11.84IIVLIDD129 pKa = 3.36MQPNGALPAITDD141 pKa = 3.47ILKK144 pKa = 9.89EE145 pKa = 3.99QFPSSQLNINNRR157 pKa = 11.84DD158 pKa = 3.12RR159 pKa = 11.84FKK161 pKa = 10.69ILKK164 pKa = 9.84DD165 pKa = 2.97KK166 pKa = 10.77TYY168 pKa = 11.39ALDD171 pKa = 3.43TFIYY175 pKa = 8.99NTTATQAVGVAGRR188 pKa = 11.84TLQNFKK194 pKa = 10.5CYY196 pKa = 10.57KK197 pKa = 9.9KK198 pKa = 10.23MNLEE202 pKa = 4.29VIFNSGNAGTIADD215 pKa = 4.27TQTGALLIATIGSTASGTADD235 pKa = 3.16GNLVMSSRR243 pKa = 11.84IRR245 pKa = 11.84FLDD248 pKa = 3.39PP249 pKa = 3.84

Molecular weight: 27.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

620

249

371

310.0

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.613 ± 2.135

0.484 ± 0.178

5.0 ± 0.325

6.29 ± 2.164

4.194 ± 0.099

7.742 ± 0.286

0.968 ± 0.54

5.968 ± 0.703

5.645 ± 0.013

8.387 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.613 ± 0.444

4.677 ± 0.975

4.839 ± 0.683

4.839 ± 0.011

7.742 ± 0.162

6.935 ± 0.06

8.548 ± 0.608

4.677 ± 0.145

1.129 ± 0.63

3.71 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski