Amino acid dipepetide frequency for Pectobacterium phage MA11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.741AlaAla: 12.741 ± 2.431
0.708AlaCys: 0.708 ± 0.236
5.804AlaAsp: 5.804 ± 0.745
5.946AlaGlu: 5.946 ± 0.858
4.53AlaPhe: 4.53 ± 0.653
7.928AlaGly: 7.928 ± 0.821
0.991AlaHis: 0.991 ± 0.298
5.804AlaIle: 5.804 ± 0.946
5.309AlaLys: 5.309 ± 1.256
8.565AlaLeu: 8.565 ± 0.967
2.76AlaMet: 2.76 ± 0.566
4.53AlaAsn: 4.53 ± 0.642
4.105AlaPro: 4.105 ± 0.464
5.096AlaGln: 5.096 ± 1.053
6.937AlaArg: 6.937 ± 0.774
7.29AlaSer: 7.29 ± 0.877
5.167AlaThr: 5.167 ± 0.909
7.22AlaVal: 7.22 ± 0.758
1.416AlaTrp: 1.416 ± 0.387
3.044AlaTyr: 3.044 ± 0.443
0.0AlaXaa: 0.0 ± 0.0
Cys
0.779CysAla: 0.779 ± 0.267
0.142CysCys: 0.142 ± 0.107
0.566CysAsp: 0.566 ± 0.214
0.566CysGlu: 0.566 ± 0.169
0.425CysPhe: 0.425 ± 0.16
0.637CysGly: 0.637 ± 0.253
0.071CysHis: 0.071 ± 0.068
0.495CysIle: 0.495 ± 0.2
0.283CysLys: 0.283 ± 0.137
0.849CysLeu: 0.849 ± 0.254
0.283CysMet: 0.283 ± 0.151
0.212CysAsn: 0.212 ± 0.123
0.991CysPro: 0.991 ± 0.315
0.425CysGln: 0.425 ± 0.214
0.637CysArg: 0.637 ± 0.233
0.354CysSer: 0.354 ± 0.149
0.283CysThr: 0.283 ± 0.142
0.566CysVal: 0.566 ± 0.208
0.354CysTrp: 0.354 ± 0.17
0.354CysTyr: 0.354 ± 0.182
0.0CysXaa: 0.0 ± 0.0
Asp
6.866AspAla: 6.866 ± 0.613
0.354AspCys: 0.354 ± 0.17
5.875AspAsp: 5.875 ± 1.077
4.035AspGlu: 4.035 ± 0.818
2.76AspPhe: 2.76 ± 0.506
5.45AspGly: 5.45 ± 0.686
0.991AspHis: 0.991 ± 0.348
3.256AspIle: 3.256 ± 0.606
2.902AspLys: 2.902 ± 0.49
6.37AspLeu: 6.37 ± 0.77
2.265AspMet: 2.265 ± 0.406
2.548AspAsn: 2.548 ± 0.314
3.468AspPro: 3.468 ± 0.66
1.84AspGln: 1.84 ± 0.346
2.831AspArg: 2.831 ± 0.538
3.61AspSer: 3.61 ± 0.565
3.822AspThr: 3.822 ± 0.394
5.096AspVal: 5.096 ± 0.651
1.203AspTrp: 1.203 ± 0.284
2.973AspTyr: 2.973 ± 0.601
0.0AspXaa: 0.0 ± 0.0
Glu
6.37GluAla: 6.37 ± 0.88
0.495GluCys: 0.495 ± 0.169
4.318GluAsp: 4.318 ± 0.713
4.035GluGlu: 4.035 ± 0.73
1.982GluPhe: 1.982 ± 0.378
3.256GluGly: 3.256 ± 0.476
0.779GluHis: 0.779 ± 0.243
3.468GluIle: 3.468 ± 0.545
2.194GluLys: 2.194 ± 0.504
5.663GluLeu: 5.663 ± 0.735
1.699GluMet: 1.699 ± 0.345
2.265GluAsn: 2.265 ± 0.377
1.557GluPro: 1.557 ± 0.346
2.123GluGln: 2.123 ± 0.558
3.681GluArg: 3.681 ± 0.479
3.256GluSer: 3.256 ± 0.447
3.468GluThr: 3.468 ± 0.439
3.681GluVal: 3.681 ± 0.517
1.628GluTrp: 1.628 ± 0.308
0.92GluTyr: 0.92 ± 0.26
0.0GluXaa: 0.0 ± 0.0
Phe
2.76PheAla: 2.76 ± 0.547
0.142PheCys: 0.142 ± 0.151
2.973PheAsp: 2.973 ± 0.496
2.194PheGlu: 2.194 ± 0.409
1.486PhePhe: 1.486 ± 0.316
2.902PheGly: 2.902 ± 0.421
0.566PheHis: 0.566 ± 0.172
2.831PheIle: 2.831 ± 0.501
1.628PheLys: 1.628 ± 0.446
2.265PheLeu: 2.265 ± 0.404
0.637PheMet: 0.637 ± 0.172
1.982PheAsn: 1.982 ± 0.407
1.557PhePro: 1.557 ± 0.308
1.345PheGln: 1.345 ± 0.286
2.265PheArg: 2.265 ± 0.312
2.973PheSer: 2.973 ± 0.436
2.548PheThr: 2.548 ± 0.511
2.407PheVal: 2.407 ± 0.329
0.849PheTrp: 0.849 ± 0.227
1.274PheTyr: 1.274 ± 0.342
0.0PheXaa: 0.0 ± 0.0
Gly
6.583GlyAla: 6.583 ± 0.876
1.062GlyCys: 1.062 ± 0.325
5.096GlyAsp: 5.096 ± 0.716
3.539GlyGlu: 3.539 ± 0.648
2.69GlyPhe: 2.69 ± 0.393
5.946GlyGly: 5.946 ± 0.69
0.92GlyHis: 0.92 ± 0.245
3.539GlyIle: 3.539 ± 0.483
3.822GlyLys: 3.822 ± 0.709
5.875GlyLeu: 5.875 ± 0.754
1.557GlyMet: 1.557 ± 0.359
2.053GlyAsn: 2.053 ± 0.342
2.194GlyPro: 2.194 ± 0.511
3.681GlyGln: 3.681 ± 0.335
4.601GlyArg: 4.601 ± 0.676
5.096GlySer: 5.096 ± 0.726
5.025GlyThr: 5.025 ± 0.599
4.53GlyVal: 4.53 ± 0.556
1.557GlyTrp: 1.557 ± 0.44
2.69GlyTyr: 2.69 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.345HisAla: 1.345 ± 0.358
0.283HisCys: 0.283 ± 0.157
1.274HisAsp: 1.274 ± 0.308
0.425HisGlu: 0.425 ± 0.183
0.566HisPhe: 0.566 ± 0.213
1.557HisGly: 1.557 ± 0.377
0.637HisHis: 0.637 ± 0.225
0.991HisIle: 0.991 ± 0.371
0.849HisLys: 0.849 ± 0.257
1.416HisLeu: 1.416 ± 0.317
0.142HisMet: 0.142 ± 0.099
0.354HisAsn: 0.354 ± 0.192
0.849HisPro: 0.849 ± 0.235
0.566HisGln: 0.566 ± 0.189
0.708HisArg: 0.708 ± 0.256
0.779HisSer: 0.779 ± 0.27
0.779HisThr: 0.779 ± 0.281
1.133HisVal: 1.133 ± 0.288
0.495HisTrp: 0.495 ± 0.169
0.283HisTyr: 0.283 ± 0.139
0.0HisXaa: 0.0 ± 0.0
Ile
5.025IleAla: 5.025 ± 0.635
0.708IleCys: 0.708 ± 0.256
4.742IleAsp: 4.742 ± 0.501
2.902IleGlu: 2.902 ± 0.443
1.699IlePhe: 1.699 ± 0.392
4.105IleGly: 4.105 ± 0.486
0.779IleHis: 0.779 ± 0.23
2.336IleIle: 2.336 ± 0.287
2.902IleLys: 2.902 ± 0.571
2.973IleLeu: 2.973 ± 0.465
1.133IleMet: 1.133 ± 0.291
2.902IleAsn: 2.902 ± 0.326
2.194IlePro: 2.194 ± 0.507
1.77IleGln: 1.77 ± 0.433
3.114IleArg: 3.114 ± 0.395
3.468IleSer: 3.468 ± 0.576
3.256IleThr: 3.256 ± 0.513
3.327IleVal: 3.327 ± 0.42
0.425IleTrp: 0.425 ± 0.211
2.053IleTyr: 2.053 ± 0.395
0.0IleXaa: 0.0 ± 0.0
Lys
5.946LysAla: 5.946 ± 0.643
0.283LysCys: 0.283 ± 0.176
2.973LysAsp: 2.973 ± 0.615
2.76LysGlu: 2.76 ± 0.629
1.416LysPhe: 1.416 ± 0.27
3.114LysGly: 3.114 ± 0.612
0.637LysHis: 0.637 ± 0.244
2.76LysIle: 2.76 ± 0.398
3.893LysLys: 3.893 ± 0.719
4.318LysLeu: 4.318 ± 0.449
1.628LysMet: 1.628 ± 0.385
1.982LysAsn: 1.982 ± 0.36
2.69LysPro: 2.69 ± 0.572
1.628LysGln: 1.628 ± 0.469
3.539LysArg: 3.539 ± 0.785
2.548LysSer: 2.548 ± 0.416
4.035LysThr: 4.035 ± 0.668
2.619LysVal: 2.619 ± 0.464
1.203LysTrp: 1.203 ± 0.38
1.486LysTyr: 1.486 ± 0.323
0.0LysXaa: 0.0 ± 0.0
Leu
8.423LeuAla: 8.423 ± 0.728
0.495LeuCys: 0.495 ± 0.208
4.813LeuAsp: 4.813 ± 0.543
4.601LeuGlu: 4.601 ± 0.671
2.619LeuPhe: 2.619 ± 0.443
5.167LeuGly: 5.167 ± 0.58
1.274LeuHis: 1.274 ± 0.379
3.681LeuIle: 3.681 ± 0.41
5.592LeuLys: 5.592 ± 0.991
5.309LeuLeu: 5.309 ± 0.663
2.477LeuMet: 2.477 ± 0.397
3.61LeuAsn: 3.61 ± 0.527
3.964LeuPro: 3.964 ± 0.745
4.247LeuGln: 4.247 ± 0.65
3.468LeuArg: 3.468 ± 0.53
5.379LeuSer: 5.379 ± 0.606
6.229LeuThr: 6.229 ± 0.605
5.167LeuVal: 5.167 ± 0.526
1.203LeuTrp: 1.203 ± 0.378
2.69LeuTyr: 2.69 ± 0.613
0.0LeuXaa: 0.0 ± 0.0
Met
4.955MetAla: 4.955 ± 0.767
0.283MetCys: 0.283 ± 0.146
1.699MetAsp: 1.699 ± 0.377
0.708MetGlu: 0.708 ± 0.19
0.566MetPhe: 0.566 ± 0.179
1.486MetGly: 1.486 ± 0.359
0.637MetHis: 0.637 ± 0.236
1.203MetIle: 1.203 ± 0.271
1.628MetLys: 1.628 ± 0.283
1.557MetLeu: 1.557 ± 0.399
1.062MetMet: 1.062 ± 0.288
0.708MetAsn: 0.708 ± 0.164
1.486MetPro: 1.486 ± 0.266
1.345MetGln: 1.345 ± 0.244
1.345MetArg: 1.345 ± 0.345
2.265MetSer: 2.265 ± 0.297
1.557MetThr: 1.557 ± 0.357
1.486MetVal: 1.486 ± 0.347
0.212MetTrp: 0.212 ± 0.237
0.495MetTyr: 0.495 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
5.096AsnAla: 5.096 ± 0.702
0.212AsnCys: 0.212 ± 0.119
2.831AsnAsp: 2.831 ± 0.375
2.123AsnGlu: 2.123 ± 0.404
1.486AsnPhe: 1.486 ± 0.264
3.256AsnGly: 3.256 ± 0.439
0.708AsnHis: 0.708 ± 0.228
1.557AsnIle: 1.557 ± 0.322
1.557AsnLys: 1.557 ± 0.456
3.114AsnLeu: 3.114 ± 0.4
0.92AsnMet: 0.92 ± 0.295
1.911AsnAsn: 1.911 ± 0.373
2.619AsnPro: 2.619 ± 0.442
1.557AsnGln: 1.557 ± 0.396
2.336AsnArg: 2.336 ± 0.347
2.407AsnSer: 2.407 ± 0.327
2.902AsnThr: 2.902 ± 0.433
2.194AsnVal: 2.194 ± 0.397
0.849AsnTrp: 0.849 ± 0.213
1.062AsnTyr: 1.062 ± 0.263
0.0AsnXaa: 0.0 ± 0.0
Pro
4.388ProAla: 4.388 ± 0.69
0.566ProCys: 0.566 ± 0.296
3.185ProAsp: 3.185 ± 0.523
2.619ProGlu: 2.619 ± 0.478
1.911ProPhe: 1.911 ± 0.362
3.964ProGly: 3.964 ± 0.382
1.203ProHis: 1.203 ± 0.299
1.982ProIle: 1.982 ± 0.489
1.84ProLys: 1.84 ± 0.285
2.123ProLeu: 2.123 ± 0.426
0.92ProMet: 0.92 ± 0.267
2.336ProAsn: 2.336 ± 0.322
1.628ProPro: 1.628 ± 0.353
1.345ProGln: 1.345 ± 0.286
1.911ProArg: 1.911 ± 0.351
3.327ProSer: 3.327 ± 0.584
2.831ProThr: 2.831 ± 0.59
3.61ProVal: 3.61 ± 0.536
0.708ProTrp: 0.708 ± 0.228
2.053ProTyr: 2.053 ± 0.434
0.0ProXaa: 0.0 ± 0.0
Gln
4.813GlnAla: 4.813 ± 0.971
0.637GlnCys: 0.637 ± 0.248
2.194GlnAsp: 2.194 ± 0.369
2.76GlnGlu: 2.76 ± 0.475
1.699GlnPhe: 1.699 ± 0.348
2.477GlnGly: 2.477 ± 0.353
0.566GlnHis: 0.566 ± 0.222
1.699GlnIle: 1.699 ± 0.246
2.053GlnLys: 2.053 ± 0.475
3.681GlnLeu: 3.681 ± 0.623
1.345GlnMet: 1.345 ± 0.394
1.203GlnAsn: 1.203 ± 0.224
1.911GlnPro: 1.911 ± 0.288
2.265GlnGln: 2.265 ± 0.69
2.831GlnArg: 2.831 ± 0.363
1.77GlnSer: 1.77 ± 0.48
2.548GlnThr: 2.548 ± 0.456
2.477GlnVal: 2.477 ± 0.395
0.566GlnTrp: 0.566 ± 0.187
1.416GlnTyr: 1.416 ± 0.325
0.0GlnXaa: 0.0 ± 0.0
Arg
4.388ArgAla: 4.388 ± 0.51
0.637ArgCys: 0.637 ± 0.229
3.822ArgAsp: 3.822 ± 0.594
4.247ArgGlu: 4.247 ± 0.599
2.619ArgPhe: 2.619 ± 0.376
3.61ArgGly: 3.61 ± 0.596
1.274ArgHis: 1.274 ± 0.292
3.61ArgIle: 3.61 ± 0.492
2.619ArgLys: 2.619 ± 0.462
5.946ArgLeu: 5.946 ± 0.634
1.911ArgMet: 1.911 ± 0.329
2.548ArgAsn: 2.548 ± 0.356
2.123ArgPro: 2.123 ± 0.414
2.902ArgGln: 2.902 ± 0.714
4.53ArgArg: 4.53 ± 0.548
2.619ArgSer: 2.619 ± 0.422
2.619ArgThr: 2.619 ± 0.506
3.681ArgVal: 3.681 ± 0.42
0.566ArgTrp: 0.566 ± 0.183
1.486ArgTyr: 1.486 ± 0.305
0.0ArgXaa: 0.0 ± 0.0
Ser
6.724SerAla: 6.724 ± 0.837
0.637SerCys: 0.637 ± 0.278
4.035SerAsp: 4.035 ± 0.643
3.539SerGlu: 3.539 ± 0.58
2.69SerPhe: 2.69 ± 0.465
5.592SerGly: 5.592 ± 0.686
0.849SerHis: 0.849 ± 0.297
3.822SerIle: 3.822 ± 0.555
2.619SerLys: 2.619 ± 0.418
5.946SerLeu: 5.946 ± 0.741
0.92SerMet: 0.92 ± 0.233
1.84SerAsn: 1.84 ± 0.331
2.194SerPro: 2.194 ± 0.366
1.982SerGln: 1.982 ± 0.374
2.69SerArg: 2.69 ± 0.447
3.751SerSer: 3.751 ± 0.574
3.751SerThr: 3.751 ± 0.511
4.884SerVal: 4.884 ± 0.705
1.203SerTrp: 1.203 ± 0.251
3.114SerTyr: 3.114 ± 0.471
0.0SerXaa: 0.0 ± 0.0
Thr
6.512ThrAla: 6.512 ± 1.531
0.637ThrCys: 0.637 ± 0.228
3.327ThrAsp: 3.327 ± 0.44
2.973ThrGlu: 2.973 ± 0.547
3.044ThrPhe: 3.044 ± 0.437
4.742ThrGly: 4.742 ± 0.611
0.566ThrHis: 0.566 ± 0.204
2.902ThrIle: 2.902 ± 0.373
3.681ThrLys: 3.681 ± 0.42
5.45ThrLeu: 5.45 ± 0.822
1.345ThrMet: 1.345 ± 0.302
2.477ThrAsn: 2.477 ± 0.433
3.61ThrPro: 3.61 ± 0.528
1.911ThrGln: 1.911 ± 0.296
2.973ThrArg: 2.973 ± 0.561
4.105ThrSer: 4.105 ± 0.767
4.388ThrThr: 4.388 ± 0.698
4.459ThrVal: 4.459 ± 0.516
1.345ThrTrp: 1.345 ± 0.233
1.416ThrTyr: 1.416 ± 0.259
0.0ThrXaa: 0.0 ± 0.0
Val
7.857ValAla: 7.857 ± 0.683
0.566ValCys: 0.566 ± 0.234
5.309ValAsp: 5.309 ± 0.739
4.176ValGlu: 4.176 ± 0.564
1.982ValPhe: 1.982 ± 0.487
3.964ValGly: 3.964 ± 0.614
0.849ValHis: 0.849 ± 0.289
3.185ValIle: 3.185 ± 0.475
3.256ValLys: 3.256 ± 0.432
5.309ValLeu: 5.309 ± 0.765
2.123ValMet: 2.123 ± 0.377
2.831ValAsn: 2.831 ± 0.356
2.548ValPro: 2.548 ± 0.453
3.185ValGln: 3.185 ± 0.452
4.176ValArg: 4.176 ± 0.58
4.53ValSer: 4.53 ± 0.546
4.105ValThr: 4.105 ± 0.693
3.893ValVal: 3.893 ± 0.719
0.779ValTrp: 0.779 ± 0.221
1.77ValTyr: 1.77 ± 0.321
0.0ValXaa: 0.0 ± 0.0
Trp
1.77TrpAla: 1.77 ± 0.591
0.142TrpCys: 0.142 ± 0.115
1.133TrpAsp: 1.133 ± 0.283
0.991TrpGlu: 0.991 ± 0.289
0.495TrpPhe: 0.495 ± 0.203
0.637TrpGly: 0.637 ± 0.172
0.425TrpHis: 0.425 ± 0.182
1.133TrpIle: 1.133 ± 0.22
1.062TrpLys: 1.062 ± 0.376
1.557TrpLeu: 1.557 ± 0.349
0.779TrpMet: 0.779 ± 0.29
0.637TrpAsn: 0.637 ± 0.217
1.416TrpPro: 1.416 ± 0.335
0.354TrpGln: 0.354 ± 0.164
0.991TrpArg: 0.991 ± 0.356
0.849TrpSer: 0.849 ± 0.223
1.062TrpThr: 1.062 ± 0.266
1.486TrpVal: 1.486 ± 0.27
0.566TrpTrp: 0.566 ± 0.21
0.212TrpTyr: 0.212 ± 0.098
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.548TyrAla: 2.548 ± 0.433
0.283TyrCys: 0.283 ± 0.167
2.619TyrAsp: 2.619 ± 0.471
1.699TyrGlu: 1.699 ± 0.416
0.92TyrPhe: 0.92 ± 0.21
2.265TyrGly: 2.265 ± 0.458
0.708TyrHis: 0.708 ± 0.219
1.557TyrIle: 1.557 ± 0.337
1.77TyrLys: 1.77 ± 0.35
2.123TyrLeu: 2.123 ± 0.518
0.708TyrMet: 0.708 ± 0.212
1.77TyrAsn: 1.77 ± 0.285
1.345TyrPro: 1.345 ± 0.302
1.345TyrGln: 1.345 ± 0.338
2.194TyrArg: 2.194 ± 0.412
2.407TyrSer: 2.407 ± 0.375
1.486TyrThr: 1.486 ± 0.242
2.477TyrVal: 2.477 ± 0.642
0.566TyrTrp: 0.566 ± 0.199
0.708TyrTyr: 0.708 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (14129 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski