Amino acid dipepetide frequency for Pseudoruegeria sp. SK021

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.736AlaAla: 16.736 ± 0.171
1.162AlaCys: 1.162 ± 0.033
7.27AlaAsp: 7.27 ± 0.089
7.117AlaGlu: 7.117 ± 0.086
4.358AlaPhe: 4.358 ± 0.059
10.188AlaGly: 10.188 ± 0.107
2.286AlaHis: 2.286 ± 0.051
6.219AlaIle: 6.219 ± 0.081
3.524AlaLys: 3.524 ± 0.066
13.637AlaLeu: 13.637 ± 0.128
3.666AlaMet: 3.666 ± 0.06
2.712AlaAsn: 2.712 ± 0.044
5.857AlaPro: 5.857 ± 0.084
4.483AlaGln: 4.483 ± 0.07
8.274AlaArg: 8.274 ± 0.102
5.966AlaSer: 5.966 ± 0.066
6.476AlaThr: 6.476 ± 0.084
8.712AlaVal: 8.712 ± 0.099
1.415AlaTrp: 1.415 ± 0.041
2.534AlaTyr: 2.534 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.095CysAla: 1.095 ± 0.035
0.121CysCys: 0.121 ± 0.011
0.619CysAsp: 0.619 ± 0.024
0.405CysGlu: 0.405 ± 0.018
0.35CysPhe: 0.35 ± 0.018
0.964CysGly: 0.964 ± 0.035
0.255CysHis: 0.255 ± 0.017
0.419CysIle: 0.419 ± 0.019
0.202CysLys: 0.202 ± 0.012
0.98CysLeu: 0.98 ± 0.031
0.183CysMet: 0.183 ± 0.013
0.236CysAsn: 0.236 ± 0.016
0.492CysPro: 0.492 ± 0.023
0.257CysGln: 0.257 ± 0.014
0.581CysArg: 0.581 ± 0.025
0.459CysSer: 0.459 ± 0.021
0.459CysThr: 0.459 ± 0.02
0.619CysVal: 0.619 ± 0.026
0.139CysTrp: 0.139 ± 0.01
0.19CysTyr: 0.19 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.45AspAla: 7.45 ± 0.102
0.494AspCys: 0.494 ± 0.022
3.474AspAsp: 3.474 ± 0.074
3.089AspGlu: 3.089 ± 0.064
2.269AspPhe: 2.269 ± 0.049
5.918AspGly: 5.918 ± 0.1
1.384AspHis: 1.384 ± 0.044
3.132AspIle: 3.132 ± 0.053
1.535AspLys: 1.535 ± 0.043
7.152AspLeu: 7.152 ± 0.096
1.542AspMet: 1.542 ± 0.038
1.306AspAsn: 1.306 ± 0.037
4.047AspPro: 4.047 ± 0.065
2.12AspGln: 2.12 ± 0.046
4.538AspArg: 4.538 ± 0.067
2.434AspSer: 2.434 ± 0.048
3.203AspThr: 3.203 ± 0.069
4.297AspVal: 4.297 ± 0.072
1.232AspTrp: 1.232 ± 0.035
1.533AspTyr: 1.533 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
6.923GluAla: 6.923 ± 0.082
0.342GluCys: 0.342 ± 0.017
3.019GluAsp: 3.019 ± 0.062
2.422GluGlu: 2.422 ± 0.061
1.719GluPhe: 1.719 ± 0.036
3.813GluGly: 3.813 ± 0.062
0.97GluHis: 0.97 ± 0.028
3.421GluIle: 3.421 ± 0.061
1.576GluLys: 1.576 ± 0.041
4.609GluLeu: 4.609 ± 0.065
1.579GluMet: 1.579 ± 0.039
1.478GluAsn: 1.478 ± 0.041
2.19GluPro: 2.19 ± 0.051
1.822GluGln: 1.822 ± 0.044
3.485GluArg: 3.485 ± 0.056
1.992GluSer: 1.992 ± 0.042
3.645GluThr: 3.645 ± 0.058
3.922GluVal: 3.922 ± 0.057
0.579GluTrp: 0.579 ± 0.024
0.932GluTyr: 0.932 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
4.334PheAla: 4.334 ± 0.067
0.441PheCys: 0.441 ± 0.019
2.876PheAsp: 2.876 ± 0.046
1.954PheGlu: 1.954 ± 0.044
1.445PhePhe: 1.445 ± 0.039
3.809PheGly: 3.809 ± 0.067
0.789PheHis: 0.789 ± 0.031
1.692PheIle: 1.692 ± 0.043
0.873PheLys: 0.873 ± 0.027
3.632PheLeu: 3.632 ± 0.069
0.812PheMet: 0.812 ± 0.027
1.081PheAsn: 1.081 ± 0.037
1.703PhePro: 1.703 ± 0.036
1.149PheGln: 1.149 ± 0.034
2.135PheArg: 2.135 ± 0.044
2.435PheSer: 2.435 ± 0.045
2.111PheThr: 2.111 ± 0.05
2.791PheVal: 2.791 ± 0.057
0.599PheTrp: 0.599 ± 0.024
0.9PheTyr: 0.9 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
9.683GlyAla: 9.683 ± 0.105
0.906GlyCys: 0.906 ± 0.031
4.577GlyAsp: 4.577 ± 0.076
3.822GlyGlu: 3.822 ± 0.067
3.659GlyPhe: 3.659 ± 0.06
7.163GlyGly: 7.163 ± 0.104
1.854GlyHis: 1.854 ± 0.039
4.617GlyIle: 4.617 ± 0.07
2.905GlyLys: 2.905 ± 0.055
9.732GlyLeu: 9.732 ± 0.105
2.535GlyMet: 2.535 ± 0.051
2.082GlyAsn: 2.082 ± 0.051
3.939GlyPro: 3.939 ± 0.058
3.301GlyGln: 3.301 ± 0.063
5.66GlyArg: 5.66 ± 0.069
4.348GlySer: 4.348 ± 0.077
5.011GlyThr: 5.011 ± 0.094
6.446GlyVal: 6.446 ± 0.074
1.419GlyTrp: 1.419 ± 0.039
2.248GlyTyr: 2.248 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.248HisAla: 2.248 ± 0.049
0.243HisCys: 0.243 ± 0.015
1.367HisAsp: 1.367 ± 0.041
0.873HisGlu: 0.873 ± 0.026
0.762HisPhe: 0.762 ± 0.029
1.844HisGly: 1.844 ± 0.042
0.558HisHis: 0.558 ± 0.024
0.939HisIle: 0.939 ± 0.027
0.51HisLys: 0.51 ± 0.02
2.193HisLeu: 2.193 ± 0.048
0.519HisMet: 0.519 ± 0.021
0.46HisAsn: 0.46 ± 0.018
1.429HisPro: 1.429 ± 0.04
0.606HisGln: 0.606 ± 0.027
1.381HisArg: 1.381 ± 0.036
0.984HisSer: 0.984 ± 0.03
0.832HisThr: 0.832 ± 0.029
1.471HisVal: 1.471 ± 0.037
0.35HisTrp: 0.35 ± 0.018
0.49HisTyr: 0.49 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.148IleAla: 7.148 ± 0.084
0.619IleCys: 0.619 ± 0.021
3.746IleAsp: 3.746 ± 0.064
3.093IleGlu: 3.093 ± 0.057
1.874IlePhe: 1.874 ± 0.041
5.162IleGly: 5.162 ± 0.075
0.906IleHis: 0.906 ± 0.029
2.236IleIle: 2.236 ± 0.057
1.417IleLys: 1.417 ± 0.033
5.146IleLeu: 5.146 ± 0.09
1.083IleMet: 1.083 ± 0.031
1.396IleAsn: 1.396 ± 0.039
2.478IlePro: 2.478 ± 0.054
1.285IleGln: 1.285 ± 0.032
3.167IleArg: 3.167 ± 0.058
3.141IleSer: 3.141 ± 0.053
3.063IleThr: 3.063 ± 0.057
4.027IleVal: 4.027 ± 0.063
0.767IleTrp: 0.767 ± 0.026
1.18IleTyr: 1.18 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
3.56LysAla: 3.56 ± 0.065
0.166LysCys: 0.166 ± 0.012
1.617LysAsp: 1.617 ± 0.038
1.175LysGlu: 1.175 ± 0.036
0.902LysPhe: 0.902 ± 0.031
2.302LysGly: 2.302 ± 0.046
0.527LysHis: 0.527 ± 0.024
1.66LysIle: 1.66 ± 0.039
0.985LysLys: 0.985 ± 0.037
2.773LysLeu: 2.773 ± 0.051
0.841LysMet: 0.841 ± 0.025
0.714LysAsn: 0.714 ± 0.023
1.635LysPro: 1.635 ± 0.038
0.805LysGln: 0.805 ± 0.027
1.977LysArg: 1.977 ± 0.046
1.86LysSer: 1.86 ± 0.051
1.93LysThr: 1.93 ± 0.047
2.125LysVal: 2.125 ± 0.053
0.346LysTrp: 0.346 ± 0.021
0.589LysTyr: 0.589 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
13.304LeuAla: 13.304 ± 0.135
1.014LeuCys: 1.014 ± 0.032
6.345LeuAsp: 6.345 ± 0.079
4.931LeuGlu: 4.931 ± 0.063
3.627LeuPhe: 3.627 ± 0.069
8.57LeuGly: 8.57 ± 0.113
1.912LeuHis: 1.912 ± 0.043
5.635LeuIle: 5.635 ± 0.091
3.01LeuLys: 3.01 ± 0.056
9.36LeuLeu: 9.36 ± 0.125
2.684LeuMet: 2.684 ± 0.059
2.799LeuAsn: 2.799 ± 0.052
5.86LeuPro: 5.86 ± 0.08
2.891LeuGln: 2.891 ± 0.054
7.151LeuArg: 7.151 ± 0.103
7.273LeuSer: 7.273 ± 0.086
6.723LeuThr: 6.723 ± 0.094
7.132LeuVal: 7.132 ± 0.1
1.325LeuTrp: 1.325 ± 0.037
2.032LeuTyr: 2.032 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
3.345MetAla: 3.345 ± 0.051
0.18MetCys: 0.18 ± 0.012
1.477MetAsp: 1.477 ± 0.034
1.064MetGlu: 1.064 ± 0.039
0.865MetPhe: 0.865 ± 0.027
2.191MetGly: 2.191 ± 0.043
0.429MetHis: 0.429 ± 0.02
1.667MetIle: 1.667 ± 0.036
0.865MetLys: 0.865 ± 0.03
2.522MetLeu: 2.522 ± 0.046
0.748MetMet: 0.748 ± 0.029
0.813MetAsn: 0.813 ± 0.024
1.453MetPro: 1.453 ± 0.04
0.934MetGln: 0.934 ± 0.029
1.756MetArg: 1.756 ± 0.037
1.738MetSer: 1.738 ± 0.039
2.424MetThr: 2.424 ± 0.039
2.007MetVal: 2.007 ± 0.043
0.246MetTrp: 0.246 ± 0.016
0.341MetTyr: 0.341 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.031AsnAla: 3.031 ± 0.047
0.246AsnCys: 0.246 ± 0.014
1.444AsnAsp: 1.444 ± 0.039
1.047AsnGlu: 1.047 ± 0.029
0.995AsnPhe: 0.995 ± 0.033
2.391AsnGly: 2.391 ± 0.052
0.486AsnHis: 0.486 ± 0.02
1.349AsnIle: 1.349 ± 0.034
0.599AsnLys: 0.599 ± 0.023
2.705AsnLeu: 2.705 ± 0.059
0.628AsnMet: 0.628 ± 0.025
0.624AsnAsn: 0.624 ± 0.027
1.803AsnPro: 1.803 ± 0.034
0.768AsnGln: 0.768 ± 0.026
1.71AsnArg: 1.71 ± 0.031
1.268AsnSer: 1.268 ± 0.036
1.415AsnThr: 1.415 ± 0.038
1.837AsnVal: 1.837 ± 0.038
0.431AsnTrp: 0.431 ± 0.022
0.663AsnTyr: 0.663 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
5.849ProAla: 5.849 ± 0.084
0.365ProCys: 0.365 ± 0.021
4.407ProAsp: 4.407 ± 0.07
3.868ProGlu: 3.868 ± 0.07
1.955ProPhe: 1.955 ± 0.047
4.481ProGly: 4.481 ± 0.066
1.048ProHis: 1.048 ± 0.03
2.44ProIle: 2.44 ± 0.048
1.594ProLys: 1.594 ± 0.039
4.972ProLeu: 4.972 ± 0.083
1.311ProMet: 1.311 ± 0.025
1.384ProAsn: 1.384 ± 0.034
2.414ProPro: 2.414 ± 0.061
1.716ProGln: 1.716 ± 0.045
2.78ProArg: 2.78 ± 0.061
3.0ProSer: 3.0 ± 0.058
2.687ProThr: 2.687 ± 0.057
4.477ProVal: 4.477 ± 0.065
0.684ProTrp: 0.684 ± 0.025
1.226ProTyr: 1.226 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.207GlnAla: 4.207 ± 0.077
0.213GlnCys: 0.213 ± 0.013
1.847GlnAsp: 1.847 ± 0.047
1.392GlnGlu: 1.392 ± 0.035
1.178GlnPhe: 1.178 ± 0.033
2.561GlnGly: 2.561 ± 0.055
0.629GlnHis: 0.629 ± 0.021
2.225GlnIle: 2.225 ± 0.04
0.947GlnLys: 0.947 ± 0.032
2.842GlnLeu: 2.842 ± 0.057
1.114GlnMet: 1.114 ± 0.027
0.922GlnAsn: 0.922 ± 0.03
1.677GlnPro: 1.677 ± 0.036
1.219GlnGln: 1.219 ± 0.036
2.279GlnArg: 2.279 ± 0.048
2.082GlnSer: 2.082 ± 0.047
2.12GlnThr: 2.12 ± 0.048
2.561GlnVal: 2.561 ± 0.044
0.372GlnTrp: 0.372 ± 0.019
0.592GlnTyr: 0.592 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
7.589ArgAla: 7.589 ± 0.094
0.52ArgCys: 0.52 ± 0.022
4.306ArgAsp: 4.306 ± 0.067
3.121ArgGlu: 3.121 ± 0.054
2.605ArgPhe: 2.605 ± 0.048
4.405ArgGly: 4.405 ± 0.066
1.637ArgHis: 1.637 ± 0.038
3.831ArgIle: 3.831 ± 0.059
2.092ArgLys: 2.092 ± 0.044
7.254ArgLeu: 7.254 ± 0.091
1.987ArgMet: 1.987 ± 0.042
1.771ArgAsn: 1.771 ± 0.041
3.389ArgPro: 3.389 ± 0.058
2.447ArgGln: 2.447 ± 0.053
4.956ArgArg: 4.956 ± 0.085
3.473ArgSer: 3.473 ± 0.053
3.296ArgThr: 3.296 ± 0.056
4.607ArgVal: 4.607 ± 0.058
0.933ArgTrp: 0.933 ± 0.031
1.48ArgTyr: 1.48 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
6.236SerAla: 6.236 ± 0.072
0.426SerCys: 0.426 ± 0.017
3.643SerAsp: 3.643 ± 0.055
2.751SerGlu: 2.751 ± 0.054
2.279SerPhe: 2.279 ± 0.047
5.843SerGly: 5.843 ± 0.077
1.123SerHis: 1.123 ± 0.034
2.669SerIle: 2.669 ± 0.052
1.515SerLys: 1.515 ± 0.036
5.638SerLeu: 5.638 ± 0.076
1.475SerMet: 1.475 ± 0.034
1.408SerAsn: 1.408 ± 0.034
2.802SerPro: 2.802 ± 0.051
1.762SerGln: 1.762 ± 0.042
3.404SerArg: 3.404 ± 0.057
2.743SerSer: 2.743 ± 0.054
2.85SerThr: 2.85 ± 0.056
4.135SerVal: 4.135 ± 0.066
0.792SerTrp: 0.792 ± 0.022
1.34SerTyr: 1.34 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
7.006ThrAla: 7.006 ± 0.098
0.529ThrCys: 0.529 ± 0.023
3.468ThrAsp: 3.468 ± 0.066
2.963ThrGlu: 2.963 ± 0.056
2.181ThrPhe: 2.181 ± 0.055
5.732ThrGly: 5.732 ± 0.064
1.149ThrHis: 1.149 ± 0.028
2.839ThrIle: 2.839 ± 0.056
1.479ThrLys: 1.479 ± 0.042
6.579ThrLeu: 6.579 ± 0.082
1.372ThrMet: 1.372 ± 0.034
1.328ThrAsn: 1.328 ± 0.035
3.742ThrPro: 3.742 ± 0.058
1.76ThrGln: 1.76 ± 0.041
3.503ThrArg: 3.503 ± 0.059
3.075ThrSer: 3.075 ± 0.048
3.116ThrThr: 3.116 ± 0.068
4.638ThrVal: 4.638 ± 0.079
0.705ThrTrp: 0.705 ± 0.026
1.268ThrTyr: 1.268 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
9.004ValAla: 9.004 ± 0.107
0.651ValCys: 0.651 ± 0.025
4.25ValAsp: 4.25 ± 0.062
3.768ValGlu: 3.768 ± 0.056
3.051ValPhe: 3.051 ± 0.054
5.5ValGly: 5.5 ± 0.08
1.32ValHis: 1.32 ± 0.034
4.358ValIle: 4.358 ± 0.076
1.925ValLys: 1.925 ± 0.045
7.896ValLeu: 7.896 ± 0.085
2.103ValMet: 2.103 ± 0.047
1.888ValAsn: 1.888 ± 0.043
3.889ValPro: 3.889 ± 0.063
2.333ValGln: 2.333 ± 0.045
4.338ValArg: 4.338 ± 0.071
4.414ValSer: 4.414 ± 0.059
5.085ValThr: 5.085 ± 0.075
6.036ValVal: 6.036 ± 0.088
1.028ValTrp: 1.028 ± 0.033
1.483ValTyr: 1.483 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.378TrpAla: 1.378 ± 0.037
0.146TrpCys: 0.146 ± 0.011
0.787TrpAsp: 0.787 ± 0.028
0.53TrpGlu: 0.53 ± 0.023
0.563TrpPhe: 0.563 ± 0.02
0.969TrpGly: 0.969 ± 0.029
0.342TrpHis: 0.342 ± 0.016
0.679TrpIle: 0.679 ± 0.024
0.403TrpLys: 0.403 ± 0.019
1.629TrpLeu: 1.629 ± 0.039
0.431TrpMet: 0.431 ± 0.02
0.431TrpAsn: 0.431 ± 0.02
0.74TrpPro: 0.74 ± 0.031
0.62TrpGln: 0.62 ± 0.024
1.088TrpArg: 1.088 ± 0.031
0.894TrpSer: 0.894 ± 0.031
0.847TrpThr: 0.847 ± 0.029
0.956TrpVal: 0.956 ± 0.031
0.237TrpTrp: 0.237 ± 0.014
0.29TrpTyr: 0.29 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.463TyrAla: 2.463 ± 0.042
0.242TyrCys: 0.242 ± 0.014
1.608TyrAsp: 1.608 ± 0.045
1.054TyrGlu: 1.054 ± 0.035
0.925TyrPhe: 0.925 ± 0.024
2.1TyrGly: 2.1 ± 0.043
0.456TyrHis: 0.456 ± 0.019
0.907TyrIle: 0.907 ± 0.029
0.531TyrLys: 0.531 ± 0.022
2.331TyrLeu: 2.331 ± 0.041
0.451TyrMet: 0.451 ± 0.024
0.607TyrAsn: 0.607 ± 0.024
1.119TyrPro: 1.119 ± 0.031
0.703TyrGln: 0.703 ± 0.028
1.538TyrArg: 1.538 ± 0.032
1.255TyrSer: 1.255 ± 0.032
1.139TyrThr: 1.139 ± 0.036
1.522TyrVal: 1.522 ± 0.037
0.359TyrTrp: 0.359 ± 0.02
0.578TyrTyr: 0.578 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3609 proteins (1135785 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski