Amino acid dipepetide frequency for Streptococcus phage IPP53

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.426AlaAla: 4.426 ± 0.98
0.089AlaCys: 0.089 ± 0.088
5.311AlaAsp: 5.311 ± 1.197
5.93AlaGlu: 5.93 ± 0.646
3.098AlaPhe: 3.098 ± 0.474
4.426AlaGly: 4.426 ± 0.624
0.531AlaHis: 0.531 ± 0.185
4.868AlaIle: 4.868 ± 0.737
4.957AlaLys: 4.957 ± 0.745
6.284AlaLeu: 6.284 ± 0.791
1.947AlaMet: 1.947 ± 0.413
3.717AlaAsn: 3.717 ± 0.61
1.77AlaPro: 1.77 ± 0.381
2.921AlaGln: 2.921 ± 0.605
2.832AlaArg: 2.832 ± 0.499
4.16AlaSer: 4.16 ± 0.695
4.249AlaThr: 4.249 ± 0.681
4.691AlaVal: 4.691 ± 0.65
0.708AlaTrp: 0.708 ± 0.271
2.655AlaTyr: 2.655 ± 0.742
0.0AlaXaa: 0.0 ± 0.0
Cys
0.266CysAla: 0.266 ± 0.14
0.089CysCys: 0.089 ± 0.088
0.177CysAsp: 0.177 ± 0.112
0.885CysGlu: 0.885 ± 0.203
0.266CysPhe: 0.266 ± 0.148
0.531CysGly: 0.531 ± 0.251
0.089CysHis: 0.089 ± 0.11
0.443CysIle: 0.443 ± 0.164
0.266CysLys: 0.266 ± 0.155
0.266CysLeu: 0.266 ± 0.144
0.177CysMet: 0.177 ± 0.123
0.0CysAsn: 0.0 ± 0.0
0.089CysPro: 0.089 ± 0.076
0.177CysGln: 0.177 ± 0.133
0.0CysArg: 0.0 ± 0.0
0.177CysSer: 0.177 ± 0.139
0.089CysThr: 0.089 ± 0.093
0.443CysVal: 0.443 ± 0.188
0.177CysTrp: 0.177 ± 0.119
0.266CysTyr: 0.266 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
3.54AspAla: 3.54 ± 0.57
0.354AspCys: 0.354 ± 0.161
4.337AspAsp: 4.337 ± 0.725
3.54AspGlu: 3.54 ± 0.49
4.16AspPhe: 4.16 ± 0.577
6.815AspGly: 6.815 ± 0.997
0.62AspHis: 0.62 ± 0.205
5.222AspIle: 5.222 ± 0.627
5.842AspLys: 5.842 ± 0.571
4.072AspLeu: 4.072 ± 0.625
1.328AspMet: 1.328 ± 0.322
3.452AspAsn: 3.452 ± 0.54
1.239AspPro: 1.239 ± 0.317
1.593AspGln: 1.593 ± 0.359
2.832AspArg: 2.832 ± 0.476
3.363AspSer: 3.363 ± 0.664
3.54AspThr: 3.54 ± 0.461
3.186AspVal: 3.186 ± 0.641
0.531AspTrp: 0.531 ± 0.203
3.629AspTyr: 3.629 ± 0.517
0.0AspXaa: 0.0 ± 0.0
Glu
4.16GluAla: 4.16 ± 0.522
0.266GluCys: 0.266 ± 0.19
3.806GluAsp: 3.806 ± 0.793
6.373GluGlu: 6.373 ± 1.058
2.655GluPhe: 2.655 ± 0.375
2.567GluGly: 2.567 ± 0.37
1.328GluHis: 1.328 ± 0.346
5.576GluIle: 5.576 ± 0.78
6.019GluLys: 6.019 ± 0.886
7.789GluLeu: 7.789 ± 1.014
2.921GluMet: 2.921 ± 0.75
4.072GluAsn: 4.072 ± 0.533
1.859GluPro: 1.859 ± 0.454
4.691GluGln: 4.691 ± 0.638
3.363GluArg: 3.363 ± 0.586
3.363GluSer: 3.363 ± 0.644
3.717GluThr: 3.717 ± 0.508
4.337GluVal: 4.337 ± 0.666
0.443GluTrp: 0.443 ± 0.147
2.832GluTyr: 2.832 ± 0.528
0.0GluXaa: 0.0 ± 0.0
Phe
2.301PheAla: 2.301 ± 0.458
0.177PheCys: 0.177 ± 0.112
4.249PheAsp: 4.249 ± 0.626
4.249PheGlu: 4.249 ± 0.627
1.505PhePhe: 1.505 ± 0.371
2.124PheGly: 2.124 ± 0.562
0.62PheHis: 0.62 ± 0.264
2.036PheIle: 2.036 ± 0.46
2.301PheLys: 2.301 ± 0.457
2.301PheLeu: 2.301 ± 0.353
1.062PheMet: 1.062 ± 0.27
2.832PheAsn: 2.832 ± 0.484
0.974PhePro: 0.974 ± 0.373
1.77PheGln: 1.77 ± 0.521
1.682PheArg: 1.682 ± 0.386
3.717PheSer: 3.717 ± 0.695
2.921PheThr: 2.921 ± 0.721
2.478PheVal: 2.478 ± 0.407
0.177PheTrp: 0.177 ± 0.123
1.505PheTyr: 1.505 ± 0.416
0.0PheXaa: 0.0 ± 0.0
Gly
4.514GlyAla: 4.514 ± 0.829
0.443GlyCys: 0.443 ± 0.213
2.478GlyAsp: 2.478 ± 0.43
4.072GlyGlu: 4.072 ± 0.554
3.363GlyPhe: 3.363 ± 0.639
5.222GlyGly: 5.222 ± 0.725
1.593GlyHis: 1.593 ± 0.36
4.78GlyIle: 4.78 ± 0.623
4.957GlyLys: 4.957 ± 0.879
5.045GlyLeu: 5.045 ± 0.664
2.567GlyMet: 2.567 ± 0.447
3.806GlyAsn: 3.806 ± 0.759
0.443GlyPro: 0.443 ± 0.174
2.39GlyGln: 2.39 ± 0.45
3.806GlyArg: 3.806 ± 0.611
5.045GlySer: 5.045 ± 0.916
5.045GlyThr: 5.045 ± 0.927
5.576GlyVal: 5.576 ± 0.802
1.151GlyTrp: 1.151 ± 0.536
2.39GlyTyr: 2.39 ± 0.468
0.0GlyXaa: 0.0 ± 0.0
His
0.62HisAla: 0.62 ± 0.218
0.266HisCys: 0.266 ± 0.151
0.974HisAsp: 0.974 ± 0.357
1.239HisGlu: 1.239 ± 0.312
0.531HisPhe: 0.531 ± 0.17
1.239HisGly: 1.239 ± 0.331
0.354HisHis: 0.354 ± 0.176
1.505HisIle: 1.505 ± 0.456
0.974HisLys: 0.974 ± 0.33
0.974HisLeu: 0.974 ± 0.295
0.354HisMet: 0.354 ± 0.167
0.531HisAsn: 0.531 ± 0.236
0.797HisPro: 0.797 ± 0.257
0.443HisGln: 0.443 ± 0.173
0.531HisArg: 0.531 ± 0.146
1.505HisSer: 1.505 ± 0.415
1.328HisThr: 1.328 ± 0.309
0.62HisVal: 0.62 ± 0.208
0.266HisTrp: 0.266 ± 0.143
0.885HisTyr: 0.885 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
5.753IleAla: 5.753 ± 0.758
0.443IleCys: 0.443 ± 0.175
4.249IleAsp: 4.249 ± 0.6
6.107IleGlu: 6.107 ± 0.854
2.036IlePhe: 2.036 ± 0.43
5.576IleGly: 5.576 ± 0.784
1.151IleHis: 1.151 ± 0.289
4.072IleIle: 4.072 ± 0.758
7.7IleLys: 7.7 ± 0.912
3.363IleLeu: 3.363 ± 0.566
1.328IleMet: 1.328 ± 0.313
3.894IleAsn: 3.894 ± 0.449
2.301IlePro: 2.301 ± 0.493
2.744IleGln: 2.744 ± 0.476
2.213IleArg: 2.213 ± 0.406
5.399IleSer: 5.399 ± 0.558
4.249IleThr: 4.249 ± 0.549
3.806IleVal: 3.806 ± 0.645
0.62IleTrp: 0.62 ± 0.217
2.036IleTyr: 2.036 ± 0.385
0.0IleXaa: 0.0 ± 0.0
Lys
6.284LysAla: 6.284 ± 1.016
0.531LysCys: 0.531 ± 0.205
3.186LysAsp: 3.186 ± 0.525
5.93LysGlu: 5.93 ± 0.914
2.744LysPhe: 2.744 ± 0.495
6.284LysGly: 6.284 ± 0.794
1.593LysHis: 1.593 ± 0.375
6.284LysIle: 6.284 ± 0.816
5.665LysLys: 5.665 ± 0.861
6.373LysLeu: 6.373 ± 0.834
1.416LysMet: 1.416 ± 0.313
5.576LysAsn: 5.576 ± 0.747
1.947LysPro: 1.947 ± 0.396
3.717LysGln: 3.717 ± 0.564
3.098LysArg: 3.098 ± 0.54
4.78LysSer: 4.78 ± 0.584
5.576LysThr: 5.576 ± 0.632
5.665LysVal: 5.665 ± 0.632
1.328LysTrp: 1.328 ± 0.322
3.275LysTyr: 3.275 ± 0.49
0.0LysXaa: 0.0 ± 0.0
Leu
5.842LeuAla: 5.842 ± 0.781
0.266LeuCys: 0.266 ± 0.145
5.399LeuAsp: 5.399 ± 0.595
6.107LeuGlu: 6.107 ± 0.855
2.832LeuPhe: 2.832 ± 0.577
4.691LeuGly: 4.691 ± 0.623
1.328LeuHis: 1.328 ± 0.285
3.54LeuIle: 3.54 ± 0.574
7.258LeuLys: 7.258 ± 0.801
4.691LeuLeu: 4.691 ± 0.704
1.239LeuMet: 1.239 ± 0.286
5.488LeuAsn: 5.488 ± 0.531
2.567LeuPro: 2.567 ± 0.448
3.894LeuGln: 3.894 ± 0.669
2.567LeuArg: 2.567 ± 0.514
4.426LeuSer: 4.426 ± 0.509
5.134LeuThr: 5.134 ± 0.719
4.426LeuVal: 4.426 ± 0.777
0.797LeuTrp: 0.797 ± 0.295
2.39LeuTyr: 2.39 ± 0.382
0.0LeuXaa: 0.0 ± 0.0
Met
2.655MetAla: 2.655 ± 0.496
0.177MetCys: 0.177 ± 0.102
1.151MetAsp: 1.151 ± 0.257
1.682MetGlu: 1.682 ± 0.387
0.531MetPhe: 0.531 ± 0.239
0.797MetGly: 0.797 ± 0.229
0.177MetHis: 0.177 ± 0.11
1.416MetIle: 1.416 ± 0.386
2.301MetLys: 2.301 ± 0.307
1.151MetLeu: 1.151 ± 0.236
0.708MetMet: 0.708 ± 0.219
1.505MetAsn: 1.505 ± 0.387
0.797MetPro: 0.797 ± 0.297
1.416MetGln: 1.416 ± 0.39
1.77MetArg: 1.77 ± 0.395
1.505MetSer: 1.505 ± 0.526
2.478MetThr: 2.478 ± 0.445
1.151MetVal: 1.151 ± 0.341
0.177MetTrp: 0.177 ± 0.097
0.531MetTyr: 0.531 ± 0.258
0.0MetXaa: 0.0 ± 0.0
Asn
4.603AsnAla: 4.603 ± 0.638
0.177AsnCys: 0.177 ± 0.134
3.806AsnAsp: 3.806 ± 0.547
3.983AsnGlu: 3.983 ± 0.623
2.567AsnPhe: 2.567 ± 0.547
5.576AsnGly: 5.576 ± 0.846
0.974AsnHis: 0.974 ± 0.241
3.452AsnIle: 3.452 ± 0.6
4.426AsnLys: 4.426 ± 0.554
4.603AsnLeu: 4.603 ± 0.85
1.416AsnMet: 1.416 ± 0.345
4.249AsnAsn: 4.249 ± 1.033
2.124AsnPro: 2.124 ± 0.486
3.098AsnGln: 3.098 ± 0.581
2.036AsnArg: 2.036 ± 0.463
4.249AsnSer: 4.249 ± 0.61
2.744AsnThr: 2.744 ± 0.426
3.275AsnVal: 3.275 ± 0.515
0.885AsnTrp: 0.885 ± 0.265
2.124AsnTyr: 2.124 ± 0.455
0.0AsnXaa: 0.0 ± 0.0
Pro
1.593ProAla: 1.593 ± 0.37
0.177ProCys: 0.177 ± 0.117
2.301ProAsp: 2.301 ± 0.493
1.77ProGlu: 1.77 ± 0.321
1.593ProPhe: 1.593 ± 0.394
1.151ProGly: 1.151 ± 0.474
0.177ProHis: 0.177 ± 0.179
2.036ProIle: 2.036 ± 0.356
2.124ProLys: 2.124 ± 0.505
1.505ProLeu: 1.505 ± 0.386
0.354ProMet: 0.354 ± 0.202
2.213ProAsn: 2.213 ± 0.576
0.443ProPro: 0.443 ± 0.196
1.151ProGln: 1.151 ± 0.312
0.797ProArg: 0.797 ± 0.183
1.947ProSer: 1.947 ± 0.422
1.859ProThr: 1.859 ± 0.376
1.682ProVal: 1.682 ± 0.377
0.177ProTrp: 0.177 ± 0.179
1.505ProTyr: 1.505 ± 0.449
0.0ProXaa: 0.0 ± 0.0
Gln
3.275GlnAla: 3.275 ± 0.659
0.177GlnCys: 0.177 ± 0.114
1.682GlnAsp: 1.682 ± 0.413
3.009GlnGlu: 3.009 ± 0.501
1.505GlnPhe: 1.505 ± 0.417
3.806GlnGly: 3.806 ± 0.986
0.708GlnHis: 0.708 ± 0.241
2.832GlnIle: 2.832 ± 0.458
4.603GlnLys: 4.603 ± 0.876
3.363GlnLeu: 3.363 ± 0.464
1.151GlnMet: 1.151 ± 0.339
2.39GlnAsn: 2.39 ± 0.479
0.885GlnPro: 0.885 ± 0.239
2.478GlnGln: 2.478 ± 0.587
2.213GlnArg: 2.213 ± 0.397
3.098GlnSer: 3.098 ± 0.522
1.77GlnThr: 1.77 ± 0.405
2.921GlnVal: 2.921 ± 0.563
0.531GlnTrp: 0.531 ± 0.194
1.947GlnTyr: 1.947 ± 0.433
0.0GlnXaa: 0.0 ± 0.0
Arg
3.098ArgAla: 3.098 ± 0.449
0.089ArgCys: 0.089 ± 0.099
1.947ArgAsp: 1.947 ± 0.4
2.301ArgGlu: 2.301 ± 0.491
1.593ArgPhe: 1.593 ± 0.314
3.186ArgGly: 3.186 ± 0.694
0.62ArgHis: 0.62 ± 0.241
2.744ArgIle: 2.744 ± 0.422
3.452ArgLys: 3.452 ± 0.638
3.629ArgLeu: 3.629 ± 0.607
1.062ArgMet: 1.062 ± 0.285
2.301ArgAsn: 2.301 ± 0.411
1.859ArgPro: 1.859 ± 0.499
1.682ArgGln: 1.682 ± 0.394
1.859ArgArg: 1.859 ± 0.434
1.77ArgSer: 1.77 ± 0.31
2.301ArgThr: 2.301 ± 0.523
2.655ArgVal: 2.655 ± 0.381
0.974ArgTrp: 0.974 ± 0.364
1.947ArgTyr: 1.947 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
4.603SerAla: 4.603 ± 0.64
0.266SerCys: 0.266 ± 0.14
4.957SerAsp: 4.957 ± 0.847
4.514SerGlu: 4.514 ± 1.12
2.478SerPhe: 2.478 ± 0.538
3.717SerGly: 3.717 ± 0.991
1.505SerHis: 1.505 ± 0.425
3.894SerIle: 3.894 ± 0.496
3.894SerLys: 3.894 ± 0.684
4.691SerLeu: 4.691 ± 0.66
1.947SerMet: 1.947 ± 0.425
3.894SerAsn: 3.894 ± 0.61
1.505SerPro: 1.505 ± 0.369
3.098SerGln: 3.098 ± 0.501
2.036SerArg: 2.036 ± 0.333
4.426SerSer: 4.426 ± 1.023
4.957SerThr: 4.957 ± 0.726
4.691SerVal: 4.691 ± 0.816
0.797SerTrp: 0.797 ± 0.282
2.124SerTyr: 2.124 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
3.717ThrAla: 3.717 ± 0.864
0.354ThrCys: 0.354 ± 0.177
5.222ThrAsp: 5.222 ± 0.822
3.894ThrGlu: 3.894 ± 0.642
2.744ThrPhe: 2.744 ± 0.37
4.691ThrGly: 4.691 ± 0.591
0.531ThrHis: 0.531 ± 0.198
5.753ThrIle: 5.753 ± 0.536
4.603ThrLys: 4.603 ± 0.509
6.196ThrLeu: 6.196 ± 0.782
1.062ThrMet: 1.062 ± 0.275
3.806ThrAsn: 3.806 ± 0.541
2.655ThrPro: 2.655 ± 0.475
2.39ThrGln: 2.39 ± 0.444
2.567ThrArg: 2.567 ± 0.501
3.275ThrSer: 3.275 ± 0.509
3.983ThrThr: 3.983 ± 0.488
4.16ThrVal: 4.16 ± 0.661
1.328ThrTrp: 1.328 ± 0.332
1.947ThrTyr: 1.947 ± 0.399
0.0ThrXaa: 0.0 ± 0.0
Val
4.868ValAla: 4.868 ± 0.492
0.266ValCys: 0.266 ± 0.172
4.691ValAsp: 4.691 ± 0.681
4.072ValGlu: 4.072 ± 0.641
2.213ValPhe: 2.213 ± 0.384
3.54ValGly: 3.54 ± 0.542
1.151ValHis: 1.151 ± 0.341
5.222ValIle: 5.222 ± 0.852
5.665ValLys: 5.665 ± 0.654
3.275ValLeu: 3.275 ± 0.496
1.151ValMet: 1.151 ± 0.311
3.54ValAsn: 3.54 ± 0.562
0.797ValPro: 0.797 ± 0.238
2.301ValGln: 2.301 ± 0.478
1.947ValArg: 1.947 ± 0.475
4.78ValSer: 4.78 ± 0.587
5.488ValThr: 5.488 ± 0.556
4.072ValVal: 4.072 ± 0.7
0.62ValTrp: 0.62 ± 0.328
2.567ValTyr: 2.567 ± 0.473
0.0ValXaa: 0.0 ± 0.0
Trp
1.593TrpAla: 1.593 ± 0.365
0.0TrpCys: 0.0 ± 0.0
0.531TrpAsp: 0.531 ± 0.204
0.531TrpGlu: 0.531 ± 0.178
0.354TrpPhe: 0.354 ± 0.186
0.266TrpGly: 0.266 ± 0.148
0.354TrpHis: 0.354 ± 0.193
0.708TrpIle: 0.708 ± 0.294
0.974TrpLys: 0.974 ± 0.275
1.062TrpLeu: 1.062 ± 0.298
0.089TrpMet: 0.089 ± 0.097
0.708TrpAsn: 0.708 ± 0.222
0.266TrpPro: 0.266 ± 0.176
0.708TrpGln: 0.708 ± 0.258
0.531TrpArg: 0.531 ± 0.209
0.531TrpSer: 0.531 ± 0.228
0.974TrpThr: 0.974 ± 0.439
0.708TrpVal: 0.708 ± 0.239
0.266TrpTrp: 0.266 ± 0.144
1.151TrpTyr: 1.151 ± 0.64
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.859TyrAla: 1.859 ± 0.341
0.266TyrCys: 0.266 ± 0.172
2.744TyrAsp: 2.744 ± 0.45
1.947TyrGlu: 1.947 ± 0.481
2.213TyrPhe: 2.213 ± 0.494
2.567TyrGly: 2.567 ± 0.426
0.531TyrHis: 0.531 ± 0.227
2.655TyrIle: 2.655 ± 0.537
3.275TyrLys: 3.275 ± 0.57
4.249TyrLeu: 4.249 ± 0.614
0.708TyrMet: 0.708 ± 0.266
2.39TyrAsn: 2.39 ± 0.36
1.239TyrPro: 1.239 ± 0.33
1.682TyrGln: 1.682 ± 0.319
2.39TyrArg: 2.39 ± 0.438
2.478TyrSer: 2.478 ± 0.413
2.478TyrThr: 2.478 ± 0.548
1.682TyrVal: 1.682 ± 0.317
0.354TyrTrp: 0.354 ± 0.162
1.947TyrTyr: 1.947 ± 0.635
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11299 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski