Azoarcus indigens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6DXG2|A0A4R6DXG2_9RHOO PqqA peptide cyclase OS=Azoarcus indigens OX=29545 GN=pqqE PE=3 SV=1
MM1 pKa = 7.69SEE3 pKa = 4.52VIDD6 pKa = 3.84TPEE9 pKa = 3.97ILVFTDD15 pKa = 3.54SAANKK20 pKa = 9.08VRR22 pKa = 11.84EE23 pKa = 4.59LIDD26 pKa = 3.62EE27 pKa = 4.54EE28 pKa = 4.86GNPEE32 pKa = 3.75LKK34 pKa = 10.69LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.93GFTFDD54 pKa = 4.28EE55 pKa = 4.93EE56 pKa = 4.59VNEE59 pKa = 5.25DD60 pKa = 3.38DD61 pKa = 4.2TTFEE65 pKa = 4.48KK66 pKa = 10.86NGVMLLIDD74 pKa = 3.94PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.74IDD87 pKa = 3.65YY88 pKa = 11.47SEE90 pKa = 4.55GLEE93 pKa = 4.01GSQFVIRR100 pKa = 11.84NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6E772|A0A4R6E772_9RHOO Anthranilate synthase component II OS=Azoarcus indigens OX=29545 GN=C7389_104127 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.0AAAGGVMKK10 pKa = 10.47AYY12 pKa = 9.8RR13 pKa = 11.84RR14 pKa = 11.84CVFVALALTLIVVVAGAYY32 pKa = 9.69VRR34 pKa = 11.84LSDD37 pKa = 5.29AGLGCPDD44 pKa = 3.01WPGCYY49 pKa = 9.67GQLSPHH55 pKa = 6.57HH56 pKa = 6.52AADD59 pKa = 5.85DD60 pKa = 3.54ILAAHH65 pKa = 7.02AADD68 pKa = 4.29PYY70 pKa = 11.34GPVSLAKK77 pKa = 9.62AWKK80 pKa = 9.69EE81 pKa = 3.69MIHH84 pKa = 6.81RR85 pKa = 11.84YY86 pKa = 8.86LAGGLGLLLIAIAVLAWRR104 pKa = 11.84LRR106 pKa = 11.84GEE108 pKa = 4.19RR109 pKa = 11.84EE110 pKa = 3.71GAARR114 pKa = 11.84SAPVAALLLLAVVVLQGLLGKK135 pKa = 8.19WTVTLLLKK143 pKa = 10.13PAIVTAHH150 pKa = 7.09LLGGMAVLGLLAWIALRR167 pKa = 11.84ASGVRR172 pKa = 11.84RR173 pKa = 11.84VAAAPATVAALRR185 pKa = 11.84LGLLLLVGQIALGGWTSTNYY205 pKa = 10.47AALACPDD212 pKa = 3.98FPTCHH217 pKa = 6.31GSLWPEE223 pKa = 3.22TDD225 pKa = 3.07YY226 pKa = 11.74RR227 pKa = 11.84NAFHH231 pKa = 6.78VVRR234 pKa = 11.84EE235 pKa = 4.26LGMTADD241 pKa = 4.46GEE243 pKa = 4.44PLSFAALTAIHH254 pKa = 6.17WMHH257 pKa = 7.34RR258 pKa = 11.84IGAALVGGFLLVVGWRR274 pKa = 11.84LSRR277 pKa = 11.84QSSSALMGAALIGGVLLQVTIGIANVLFSLPLGLAVAHH315 pKa = 6.17NAGAALLLGILVWINHH331 pKa = 5.53GFCLRR336 pKa = 11.84TAPAGLRR343 pKa = 11.84FRR345 pKa = 11.84ARR347 pKa = 11.84PGLVRR352 pKa = 11.84IGWSAAAHH360 pKa = 5.22SHH362 pKa = 5.43GQVLPFRR369 pKa = 11.84PTAMRR374 pKa = 11.84KK375 pKa = 9.55RR376 pKa = 11.84GHH378 pKa = 6.08AA379 pKa = 3.46

Molecular weight: 39.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4840

0

4840

1613751

28

4533

333.4

36.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.37 ± 0.053

0.93 ± 0.011

5.22 ± 0.021

5.868 ± 0.039

3.453 ± 0.021

8.657 ± 0.045

2.173 ± 0.018

4.175 ± 0.026

2.753 ± 0.033

11.379 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.017

2.475 ± 0.025

5.308 ± 0.03

3.571 ± 0.02

7.576 ± 0.043

5.192 ± 0.039

4.681 ± 0.037

7.273 ± 0.025

1.399 ± 0.015

2.32 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski