Carassius auratus (Goldfish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi<

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P6LSX5|A0A6P6LSX5_CARAU LOW QUALITY PROTEIN: uncharacterized protein LOC113062173 OS=Carassius auratus OX=7957 GN=LOC113062173 PE=4 SV=1
MM1 pKa = 7.4LSSHH5 pKa = 5.8TFDD8 pKa = 4.7RR9 pKa = 11.84PAAGQTSEE17 pKa = 4.14GVGVVSSEE25 pKa = 4.27VQSQPTGSEE34 pKa = 3.79GFAPHH39 pKa = 6.91TDD41 pKa = 2.87GSQYY45 pKa = 11.31EE46 pKa = 4.37NGEE49 pKa = 4.02TAYY52 pKa = 10.47DD53 pKa = 4.24GSQIEE58 pKa = 4.45SPEE61 pKa = 3.79IPEE64 pKa = 4.5IEE66 pKa = 4.55SNGNGHH72 pKa = 6.38KK73 pKa = 10.23QLLNGGEE80 pKa = 4.19TGFTGVDD87 pKa = 2.9HH88 pKa = 6.9FMAGTSQIQEE98 pKa = 3.92GGGFDD103 pKa = 3.5SFGTSSHH110 pKa = 6.8LEE112 pKa = 3.91TTGVIDD118 pKa = 4.26QSSHH122 pKa = 6.76DD123 pKa = 3.95FLVDD127 pKa = 3.31LMGGIGEE134 pKa = 4.68SYY136 pKa = 11.15GPDD139 pKa = 3.3TQTDD143 pKa = 3.94GLGTLLDD150 pKa = 4.71LLDD153 pKa = 4.68TPPDD157 pKa = 3.67VPPTCLVTDD166 pKa = 4.48AVSTDD171 pKa = 2.89HH172 pKa = 6.74MGLAFQVDD180 pKa = 3.94SAAAGLSPGHH190 pKa = 6.91PSSGGPVLDD199 pKa = 5.32APPDD203 pKa = 4.04SPGLDD208 pKa = 3.3YY209 pKa = 11.6LFVDD213 pKa = 4.78NGNGDD218 pKa = 3.74YY219 pKa = 9.44THH221 pKa = 6.75SVSISDD227 pKa = 4.25NGAQSKK233 pKa = 10.69SPADD237 pKa = 3.69TTDD240 pKa = 2.91SSVVFDD246 pKa = 4.08TMSHH250 pKa = 6.95PDD252 pKa = 3.49HH253 pKa = 7.0FFTDD257 pKa = 4.41YY258 pKa = 11.3SDD260 pKa = 3.4GWVDD264 pKa = 4.17NNGADD269 pKa = 4.47LPEE272 pKa = 4.48TNGNGNGRR280 pKa = 11.84HH281 pKa = 5.55KK282 pKa = 10.62PVVDD286 pKa = 3.74MQKK289 pKa = 10.79GDD291 pKa = 3.69PQGVHH296 pKa = 6.67LDD298 pKa = 3.4ISGTGHH304 pKa = 6.28QDD306 pKa = 2.52AATAMHH312 pKa = 6.39HH313 pKa = 5.46TAVGALDD320 pKa = 5.28LNDD323 pKa = 4.14HH324 pKa = 5.91TLSPYY329 pKa = 10.54TDD331 pKa = 3.15TTGFDD336 pKa = 3.83GVIGAGAQTDD346 pKa = 3.72MAGAGGDD353 pKa = 3.94PATDD357 pKa = 3.68GQTLTDD363 pKa = 3.55MTGQGHH369 pKa = 7.02LAVTDD374 pKa = 3.57GMPNYY379 pKa = 9.25TADD382 pKa = 3.53SVRR385 pKa = 11.84ATGTGFTDD393 pKa = 4.13ASDD396 pKa = 3.61THH398 pKa = 8.17SSMVQTDD405 pKa = 4.1LPVTGDD411 pKa = 3.46PFTGVSSQTDD421 pKa = 3.08AMGTGQPGATEE432 pKa = 4.0QTQTAVSAGEE442 pKa = 4.04QYY444 pKa = 8.74HH445 pKa = 5.93TSGQGFEE452 pKa = 4.14GAEE455 pKa = 3.98NVEE458 pKa = 5.53LEE460 pKa = 4.49DD461 pKa = 3.53TCC463 pKa = 6.15

Molecular weight: 47.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P6QWA1|A0A6P6QWA1_CARAU LIM/homeobox protein Lhx1 OS=Carassius auratus OX=7957 GN=LOC113114903 PE=4 SV=1
MM1 pKa = 7.76ASHH4 pKa = 5.87KK5 pKa = 8.13TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51370

31595

82965

61822548

32

30013

745.2

83.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.212 ± 0.007

2.106 ± 0.007

5.292 ± 0.006

7.291 ± 0.015

3.369 ± 0.007

6.128 ± 0.014

2.595 ± 0.006

4.541 ± 0.009

5.99 ± 0.018

9.045 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.006

3.896 ± 0.006

5.847 ± 0.012

4.89 ± 0.014

5.544 ± 0.008

9.124 ± 0.014

5.862 ± 0.014

6.262 ± 0.014

1.066 ± 0.003

2.609 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski