Enterococcus phage IME-EFm5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3ULC6|A0A0M3ULC6_9CAUD Uncharacterized protein OS=Enterococcus phage IME-EFm5 OX=1718158 GN=EFm5_14 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.03EE3 pKa = 3.9LEE5 pKa = 4.29DD6 pKa = 4.08MIKK9 pKa = 10.55EE10 pKa = 4.33LISEE14 pKa = 4.37YY15 pKa = 10.15EE16 pKa = 3.98QNIKK20 pKa = 8.62PQYY23 pKa = 7.75YY24 pKa = 10.37LGMSDD29 pKa = 3.82EE30 pKa = 4.69DD31 pKa = 3.91AAAEE35 pKa = 3.97DD36 pKa = 3.86ARR38 pKa = 11.84ADD40 pKa = 3.63VYY42 pKa = 11.49SQVIQDD48 pKa = 4.81LYY50 pKa = 11.78SLII53 pKa = 4.27

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4R4M1|A0A0M4R4M1_9CAUD DUF3850 domain-containing protein OS=Enterococcus phage IME-EFm5 OX=1718158 GN=EFm5_08 PE=4 SV=1
MM1 pKa = 6.43TTFIILAKK9 pKa = 10.14RR10 pKa = 11.84HH11 pKa = 4.98TQTIQLSYY19 pKa = 10.54LAEE22 pKa = 4.09TMRR25 pKa = 11.84AAIDD29 pKa = 3.9EE30 pKa = 4.33FRR32 pKa = 11.84LTYY35 pKa = 9.75PKK37 pKa = 10.27KK38 pKa = 9.32KK39 pKa = 10.68YY40 pKa = 10.4KK41 pKa = 10.45IIDD44 pKa = 3.67CKK46 pKa = 10.82EE47 pKa = 3.6ATNYY51 pKa = 9.55NRR53 pKa = 4.44

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12914

37

1270

184.5

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.854 ± 0.467

0.55 ± 0.121

6.079 ± 0.251

7.906 ± 0.468

4.143 ± 0.275

6.342 ± 0.597

1.518 ± 0.174

6.543 ± 0.285

8.688 ± 0.399

8.324 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.181

6.288 ± 0.365

3.175 ± 0.206

3.585 ± 0.168

3.686 ± 0.159

5.451 ± 0.314

6.698 ± 0.435

6.357 ± 0.216

1.293 ± 0.105

4.824 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski