Canine feces-associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus canfam1

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221LEC1|A0A221LEC1_9VIRU Replication-associated protein OS=Canine feces-associated gemycircularvirus OX=2017709 PE=3 SV=1
MM1 pKa = 8.01PFRR4 pKa = 11.84FQAKK8 pKa = 9.66YY9 pKa = 11.01GLLTYY14 pKa = 7.94SQCGDD19 pKa = 3.94LDD21 pKa = 3.95PFSVVDD27 pKa = 3.46MLGDD31 pKa = 4.02LGAEE35 pKa = 4.07CIVGRR40 pKa = 11.84EE41 pKa = 3.95GHH43 pKa = 6.0TDD45 pKa = 3.22GGIHH49 pKa = 6.2LHH51 pKa = 6.04AFFMFEE57 pKa = 4.2RR58 pKa = 11.84KK59 pKa = 9.3FSSRR63 pKa = 11.84DD64 pKa = 3.14VRR66 pKa = 11.84VFDD69 pKa = 5.24VDD71 pKa = 3.67GQHH74 pKa = 6.73PNVRR78 pKa = 11.84SGWSTPEE85 pKa = 4.11KK86 pKa = 10.07GWDD89 pKa = 3.28YY90 pKa = 9.74ATKK93 pKa = 10.69YY94 pKa = 10.38GDD96 pKa = 3.57VVAGGLEE103 pKa = 4.04RR104 pKa = 11.84PGNRR108 pKa = 11.84VSASSSVWATILLSEE123 pKa = 4.41DD124 pKa = 3.29RR125 pKa = 11.84EE126 pKa = 4.89SFFEE130 pKa = 4.08TCEE133 pKa = 3.72RR134 pKa = 11.84LAPGTLLRR142 pKa = 11.84NFTSLRR148 pKa = 11.84CYY150 pKa = 10.73ADD152 pKa = 2.62WRR154 pKa = 11.84YY155 pKa = 9.96RR156 pKa = 11.84PEE158 pKa = 4.35PEE160 pKa = 4.67PYY162 pKa = 8.92IHH164 pKa = 7.1PPIISFEE171 pKa = 4.2TAGFPEE177 pKa = 4.96LDD179 pKa = 2.97QWVQQNLVGPRR190 pKa = 11.84VEE192 pKa = 4.43GNIGRR197 pKa = 11.84RR198 pKa = 11.84RR199 pKa = 11.84SLVLYY204 pKa = 10.49GEE206 pKa = 4.33SRR208 pKa = 11.84TGKK211 pKa = 7.35TLWARR216 pKa = 11.84SLGHH220 pKa = 6.17HH221 pKa = 7.12AYY223 pKa = 10.52FGGLFSLDD231 pKa = 3.58EE232 pKa = 4.44SVDD235 pKa = 3.77DD236 pKa = 3.42VDD238 pKa = 4.83YY239 pKa = 11.54AIFDD243 pKa = 4.01DD244 pKa = 4.22MQGGLSFFHH253 pKa = 7.23SYY255 pKa = 10.69KK256 pKa = 10.47FWLGCQLEE264 pKa = 4.98FWATDD269 pKa = 3.37KK270 pKa = 11.1YY271 pKa = 10.95RR272 pKa = 11.84HH273 pKa = 5.25KK274 pKa = 11.0QKK276 pKa = 10.54IKK278 pKa = 9.12WGRR281 pKa = 11.84PCIYY285 pKa = 10.02ISNNNPLADD294 pKa = 3.92AGVDD298 pKa = 3.86VDD300 pKa = 4.63WLMINCTFVCIDD312 pKa = 3.41QPIFRR317 pKa = 11.84ANTAA321 pKa = 3.21

Molecular weight: 36.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221LEC1|A0A221LEC1_9VIRU Replication-associated protein OS=Canine feces-associated gemycircularvirus OX=2017709 PE=3 SV=1
MM1 pKa = 7.54AYY3 pKa = 10.05SRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 8.43SAPRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84FRR16 pKa = 11.84RR17 pKa = 11.84GFKK20 pKa = 10.05RR21 pKa = 11.84RR22 pKa = 11.84SSRR25 pKa = 11.84LAYY28 pKa = 9.67RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84PYY34 pKa = 10.04VRR36 pKa = 11.84RR37 pKa = 11.84PYY39 pKa = 10.54RR40 pKa = 11.84KK41 pKa = 8.49RR42 pKa = 11.84TWTTRR47 pKa = 11.84AILNKK52 pKa = 9.05TSKK55 pKa = 10.61KK56 pKa = 9.44KK57 pKa = 9.17TDD59 pKa = 3.39TMQVWSDD66 pKa = 3.3TQTPGTYY73 pKa = 9.91VPGGAVIPAGTGAVYY88 pKa = 10.43QFLWCPTARR97 pKa = 11.84PGEE100 pKa = 4.27TSTGIDD106 pKa = 3.23GSAIDD111 pKa = 3.72KK112 pKa = 10.69AVRR115 pKa = 11.84TNTNCFMRR123 pKa = 11.84GVKK126 pKa = 10.23EE127 pKa = 4.04NVSLRR132 pKa = 11.84TNSGIAWRR140 pKa = 11.84WRR142 pKa = 11.84RR143 pKa = 11.84ICFTMKK149 pKa = 10.67GDD151 pKa = 3.76TLNFGDD157 pKa = 4.98NDD159 pKa = 4.41PDD161 pKa = 3.63TSNVARR167 pKa = 11.84QTSNGMMRR175 pKa = 11.84LVHH178 pKa = 6.46QDD180 pKa = 2.8LATIDD185 pKa = 4.19RR186 pKa = 11.84VTQVVFRR193 pKa = 11.84GQNQTDD199 pKa = 3.65WVNHH203 pKa = 6.7FIAPIDD209 pKa = 3.51TRR211 pKa = 11.84KK212 pKa = 9.85ISLRR216 pKa = 11.84FDD218 pKa = 3.28RR219 pKa = 11.84TRR221 pKa = 11.84VIQSNNDD228 pKa = 3.18SGTVRR233 pKa = 11.84YY234 pKa = 9.79YY235 pKa = 11.29KK236 pKa = 10.61DD237 pKa = 2.77WFAMNKK243 pKa = 9.54NIYY246 pKa = 10.45YY247 pKa = 10.21DD248 pKa = 3.7DD249 pKa = 4.22QEE251 pKa = 6.01NGDD254 pKa = 3.52QTFPSMFSVEE264 pKa = 4.28NKK266 pKa = 10.09QGMGDD271 pKa = 3.58YY272 pKa = 10.29YY273 pKa = 10.03IYY275 pKa = 10.43DD276 pKa = 3.79IIEE279 pKa = 4.3PQGGDD284 pKa = 3.2STDD287 pKa = 3.85AIEE290 pKa = 5.09FEE292 pKa = 4.43PQARR296 pKa = 11.84LYY298 pKa = 9.1WHH300 pKa = 7.04EE301 pKa = 4.13KK302 pKa = 9.24

Molecular weight: 35.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

623

302

321

311.5

35.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.618 ± 0.008

1.766 ± 0.536

7.384 ± 0.069

4.013 ± 0.947

5.618 ± 0.912

8.186 ± 0.856

1.926 ± 0.648

4.815 ± 0.335

3.852 ± 0.544

5.618 ± 1.832

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.509

4.334 ± 0.899

4.655 ± 0.243

3.852 ± 0.544

9.631 ± 1.82

6.421 ± 0.32

6.742 ± 1.757

5.939 ± 0.215

2.889 ± 0.167

4.494 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski