Bacteroides reticulotermitis JCM 10512

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides reticulotermitis

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4URY9|W4URY9_9BACE NADH-ubiquinone oxidoreductase chain N OS=Bacteroides reticulotermitis JCM 10512 OX=1445607 GN=JCM10512_2252 PE=4 SV=1
MM1 pKa = 7.13GTGNNGNYY9 pKa = 9.92QKK11 pKa = 11.06DD12 pKa = 4.31FYY14 pKa = 11.26RR15 pKa = 11.84FDD17 pKa = 3.7PSAASGSQWTILNGFGGQKK36 pKa = 9.71RR37 pKa = 11.84QGGTAFVINDD47 pKa = 3.46KK48 pKa = 11.16AYY50 pKa = 9.92ICGGDD55 pKa = 3.56NNGSDD60 pKa = 5.27VYY62 pKa = 11.01DD63 pKa = 4.57FWSFDD68 pKa = 3.81PAASSPWTQLRR79 pKa = 11.84DD80 pKa = 3.51IADD83 pKa = 3.66TDD85 pKa = 3.65SDD87 pKa = 3.63NDD89 pKa = 3.47YY90 pKa = 11.53DD91 pKa = 5.34DD92 pKa = 6.28DD93 pKa = 3.97YY94 pKa = 11.4TSIVRR99 pKa = 11.84TYY101 pKa = 10.71GSSFVIDD108 pKa = 3.75GKK110 pKa = 11.44GYY112 pKa = 9.83LALGQTVGGSLRR124 pKa = 11.84SNYY127 pKa = 8.94WIYY130 pKa = 11.34DD131 pKa = 3.48PSTDD135 pKa = 3.43LWDD138 pKa = 4.96GEE140 pKa = 4.58DD141 pKa = 3.46LTPFEE146 pKa = 4.27GSARR150 pKa = 11.84IKK152 pKa = 10.36AVCFSTGKK160 pKa = 10.06RR161 pKa = 11.84GVIATGGSSSSTFYY175 pKa = 11.42DD176 pKa = 4.3DD177 pKa = 4.76AWEE180 pKa = 4.31LDD182 pKa = 3.58PYY184 pKa = 11.07SYY186 pKa = 11.17EE187 pKa = 4.07EE188 pKa = 4.05EE189 pKa = 4.16

Molecular weight: 20.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4V1I0|W4V1I0_9BACE Uncharacterized protein OS=Bacteroides reticulotermitis JCM 10512 OX=1445607 GN=JCM10512_5125 PE=4 SV=1
MM1 pKa = 7.29SFSGAHH7 pKa = 6.19ANLIAFICFPSFKK20 pKa = 10.65SSTNYY25 pKa = 9.77LVKK28 pKa = 10.38SYY30 pKa = 8.55HH31 pKa = 5.93TEE33 pKa = 3.65VCPLSRR39 pKa = 11.84RR40 pKa = 11.84IMLSVSTRR48 pKa = 11.84DD49 pKa = 3.2SGSIRR54 pKa = 11.84IPSITEE60 pKa = 3.65RR61 pKa = 11.84HH62 pKa = 5.6SLFPLSCTRR71 pKa = 11.84TPCRR75 pKa = 11.84LTLRR79 pKa = 11.84LAYY82 pKa = 9.3PLEE85 pKa = 4.35RR86 pKa = 11.84SGRR89 pKa = 11.84DD90 pKa = 2.82TGLPCSVQVTNKK102 pKa = 7.8WVRR105 pKa = 11.84SCLFHH110 pKa = 6.19RR111 pKa = 11.84QCFVRR116 pKa = 11.84VTPKK120 pKa = 9.93FRR122 pKa = 11.84RR123 pKa = 11.84LSDD126 pKa = 3.64CLPFGSSPTSFGSLYY141 pKa = 8.36FTMFIRR147 pKa = 11.84QFTFVDD153 pKa = 3.68HH154 pKa = 7.02TIKK157 pKa = 10.61PSPSRR162 pKa = 11.84RR163 pKa = 11.84LMLPAVDD170 pKa = 3.52VPLRR174 pKa = 11.84FGLLLKK180 pKa = 10.95GKK182 pKa = 8.38VTLSGQLSHH191 pKa = 6.83KK192 pKa = 9.71WITHH196 pKa = 5.23YY197 pKa = 8.03CTCPP201 pKa = 3.03

Molecular weight: 22.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4841

0

4841

1526201

37

1924

315.3

35.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.985 ± 0.033

1.161 ± 0.013

5.521 ± 0.023

6.353 ± 0.029

4.506 ± 0.022

6.916 ± 0.034

1.841 ± 0.016

6.669 ± 0.034

6.52 ± 0.029

9.037 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.016

5.253 ± 0.034

3.789 ± 0.019

3.573 ± 0.018

4.586 ± 0.024

6.401 ± 0.028

5.896 ± 0.027

6.408 ± 0.028

1.374 ± 0.016

4.596 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski