Amino acid dipepetide frequency for Paenibacillus phage Diva

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.082AlaAla: 6.082 ± 1.052
0.626AlaCys: 0.626 ± 0.21
4.472AlaAsp: 4.472 ± 0.722
6.797AlaGlu: 6.797 ± 0.718
1.968AlaPhe: 1.968 ± 0.334
5.187AlaGly: 5.187 ± 0.772
1.163AlaHis: 1.163 ± 0.297
4.919AlaIle: 4.919 ± 0.903
5.366AlaLys: 5.366 ± 0.869
5.545AlaLeu: 5.545 ± 0.795
1.252AlaMet: 1.252 ± 0.3
2.951AlaAsn: 2.951 ± 0.518
1.431AlaPro: 1.431 ± 0.278
2.504AlaGln: 2.504 ± 0.5
1.878AlaArg: 1.878 ± 0.381
4.561AlaSer: 4.561 ± 0.816
4.114AlaThr: 4.114 ± 0.912
4.114AlaVal: 4.114 ± 0.569
1.342AlaTrp: 1.342 ± 0.354
2.683AlaTyr: 2.683 ± 0.443
0.0AlaXaa: 0.0 ± 0.0
Cys
0.447CysAla: 0.447 ± 0.228
0.268CysCys: 0.268 ± 0.154
0.358CysAsp: 0.358 ± 0.188
0.447CysGlu: 0.447 ± 0.232
0.179CysPhe: 0.179 ± 0.121
0.715CysGly: 0.715 ± 0.24
0.179CysHis: 0.179 ± 0.125
0.715CysIle: 0.715 ± 0.258
1.073CysLys: 1.073 ± 0.361
0.984CysLeu: 0.984 ± 0.292
0.268CysMet: 0.268 ± 0.168
0.268CysAsn: 0.268 ± 0.155
0.358CysPro: 0.358 ± 0.235
0.447CysGln: 0.447 ± 0.187
0.537CysArg: 0.537 ± 0.202
0.358CysSer: 0.358 ± 0.169
0.358CysThr: 0.358 ± 0.154
0.537CysVal: 0.537 ± 0.219
0.089CysTrp: 0.089 ± 0.095
0.268CysTyr: 0.268 ± 0.156
0.0CysXaa: 0.0 ± 0.0
Asp
4.025AspAla: 4.025 ± 0.655
0.447AspCys: 0.447 ± 0.194
4.472AspAsp: 4.472 ± 0.761
3.846AspGlu: 3.846 ± 0.754
3.041AspPhe: 3.041 ± 0.556
3.577AspGly: 3.577 ± 0.479
0.805AspHis: 0.805 ± 0.276
3.667AspIle: 3.667 ± 0.481
3.577AspLys: 3.577 ± 0.675
5.098AspLeu: 5.098 ± 0.577
2.236AspMet: 2.236 ± 0.46
1.968AspAsn: 1.968 ± 0.411
2.057AspPro: 2.057 ± 0.483
2.236AspGln: 2.236 ± 0.433
3.399AspArg: 3.399 ± 0.607
2.594AspSer: 2.594 ± 0.363
3.22AspThr: 3.22 ± 0.571
3.935AspVal: 3.935 ± 0.573
1.252AspTrp: 1.252 ± 0.364
2.057AspTyr: 2.057 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
5.187GluAla: 5.187 ± 0.715
0.358GluCys: 0.358 ± 0.156
4.74GluAsp: 4.74 ± 0.735
6.082GluGlu: 6.082 ± 0.939
2.415GluPhe: 2.415 ± 0.355
3.667GluGly: 3.667 ± 0.499
1.342GluHis: 1.342 ± 0.445
6.171GluIle: 6.171 ± 0.803
7.87GluLys: 7.87 ± 0.78
6.708GluLeu: 6.708 ± 0.915
4.472GluMet: 4.472 ± 0.576
3.309GluAsn: 3.309 ± 0.509
2.146GluPro: 2.146 ± 0.356
4.204GluGln: 4.204 ± 0.814
4.025GluArg: 4.025 ± 0.77
4.293GluSer: 4.293 ± 0.622
4.382GluThr: 4.382 ± 0.592
6.171GluVal: 6.171 ± 0.833
0.894GluTrp: 0.894 ± 0.335
3.041GluTyr: 3.041 ± 0.597
0.0GluXaa: 0.0 ± 0.0
Phe
1.789PheAla: 1.789 ± 0.392
0.715PheCys: 0.715 ± 0.224
2.236PheAsp: 2.236 ± 0.427
2.951PheGlu: 2.951 ± 0.532
0.805PhePhe: 0.805 ± 0.319
2.236PheGly: 2.236 ± 0.454
1.163PheHis: 1.163 ± 0.315
2.951PheIle: 2.951 ± 0.521
2.683PheLys: 2.683 ± 0.565
2.236PheLeu: 2.236 ± 0.433
0.984PheMet: 0.984 ± 0.306
2.057PheAsn: 2.057 ± 0.369
0.805PhePro: 0.805 ± 0.27
1.61PheGln: 1.61 ± 0.36
0.984PheArg: 0.984 ± 0.288
2.594PheSer: 2.594 ± 0.517
1.61PheThr: 1.61 ± 0.316
2.504PheVal: 2.504 ± 0.479
0.715PheTrp: 0.715 ± 0.261
1.163PheTyr: 1.163 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
3.309GlyAla: 3.309 ± 0.583
0.626GlyCys: 0.626 ± 0.232
2.773GlyAsp: 2.773 ± 0.652
5.366GlyGlu: 5.366 ± 0.799
2.057GlyPhe: 2.057 ± 0.513
3.309GlyGly: 3.309 ± 0.597
0.805GlyHis: 0.805 ± 0.273
5.992GlyIle: 5.992 ± 1.16
6.439GlyLys: 6.439 ± 0.72
4.83GlyLeu: 4.83 ± 0.941
1.878GlyMet: 1.878 ± 0.693
3.041GlyAsn: 3.041 ± 0.512
1.073GlyPro: 1.073 ± 0.311
2.773GlyGln: 2.773 ± 0.622
3.667GlyArg: 3.667 ± 0.605
4.025GlySer: 4.025 ± 0.803
2.951GlyThr: 2.951 ± 0.59
3.488GlyVal: 3.488 ± 0.572
1.163GlyTrp: 1.163 ± 0.27
2.236GlyTyr: 2.236 ± 0.495
0.0GlyXaa: 0.0 ± 0.0
His
1.431HisAla: 1.431 ± 0.346
0.268HisCys: 0.268 ± 0.151
0.447HisAsp: 0.447 ± 0.233
1.163HisGlu: 1.163 ± 0.288
0.626HisPhe: 0.626 ± 0.229
0.447HisGly: 0.447 ± 0.189
0.537HisHis: 0.537 ± 0.197
2.146HisIle: 2.146 ± 0.482
1.163HisLys: 1.163 ± 0.333
2.236HisLeu: 2.236 ± 0.475
0.626HisMet: 0.626 ± 0.243
1.163HisAsn: 1.163 ± 0.344
0.715HisPro: 0.715 ± 0.234
0.626HisGln: 0.626 ± 0.23
1.163HisArg: 1.163 ± 0.269
1.252HisSer: 1.252 ± 0.384
0.626HisThr: 0.626 ± 0.273
0.984HisVal: 0.984 ± 0.298
0.358HisTrp: 0.358 ± 0.151
0.447HisTyr: 0.447 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
4.472IleAla: 4.472 ± 0.688
0.715IleCys: 0.715 ± 0.272
4.74IleAsp: 4.74 ± 0.641
6.708IleGlu: 6.708 ± 0.684
3.13IlePhe: 3.13 ± 0.5
3.756IleGly: 3.756 ± 0.683
1.342IleHis: 1.342 ± 0.343
2.951IleIle: 2.951 ± 0.79
7.423IleLys: 7.423 ± 1.201
5.277IleLeu: 5.277 ± 0.602
1.52IleMet: 1.52 ± 0.345
2.773IleAsn: 2.773 ± 0.387
2.773IlePro: 2.773 ± 0.384
2.951IleGln: 2.951 ± 0.374
3.399IleArg: 3.399 ± 0.728
3.488IleSer: 3.488 ± 0.56
3.577IleThr: 3.577 ± 0.475
4.83IleVal: 4.83 ± 0.762
0.626IleTrp: 0.626 ± 0.28
2.594IleTyr: 2.594 ± 0.388
0.0IleXaa: 0.0 ± 0.0
Lys
6.887LysAla: 6.887 ± 0.762
0.447LysCys: 0.447 ± 0.185
4.204LysAsp: 4.204 ± 0.518
8.586LysGlu: 8.586 ± 0.858
2.057LysPhe: 2.057 ± 0.287
5.187LysGly: 5.187 ± 0.64
1.252LysHis: 1.252 ± 0.274
5.187LysIle: 5.187 ± 0.525
7.87LysLys: 7.87 ± 1.159
6.797LysLeu: 6.797 ± 0.752
3.309LysMet: 3.309 ± 0.457
4.651LysAsn: 4.651 ± 0.732
2.773LysPro: 2.773 ± 0.535
4.204LysGln: 4.204 ± 0.567
5.366LysArg: 5.366 ± 0.794
5.813LysSer: 5.813 ± 0.719
4.651LysThr: 4.651 ± 0.555
4.204LysVal: 4.204 ± 0.626
0.984LysTrp: 0.984 ± 0.304
2.415LysTyr: 2.415 ± 0.559
0.0LysXaa: 0.0 ± 0.0
Leu
6.797LeuAla: 6.797 ± 0.701
0.626LeuCys: 0.626 ± 0.261
4.919LeuAsp: 4.919 ± 0.656
6.35LeuGlu: 6.35 ± 0.932
2.594LeuPhe: 2.594 ± 0.644
4.83LeuGly: 4.83 ± 0.644
2.146LeuHis: 2.146 ± 0.44
4.74LeuIle: 4.74 ± 0.575
6.439LeuLys: 6.439 ± 0.653
5.277LeuLeu: 5.277 ± 0.883
1.61LeuMet: 1.61 ± 0.411
4.114LeuAsn: 4.114 ± 0.531
3.041LeuPro: 3.041 ± 0.477
3.846LeuGln: 3.846 ± 0.588
4.025LeuArg: 4.025 ± 0.698
6.261LeuSer: 6.261 ± 0.695
5.635LeuThr: 5.635 ± 0.774
3.577LeuVal: 3.577 ± 0.499
1.252LeuTrp: 1.252 ± 0.374
2.773LeuTyr: 2.773 ± 0.582
0.0LeuXaa: 0.0 ± 0.0
Met
2.057MetAla: 2.057 ± 0.416
0.089MetCys: 0.089 ± 0.088
1.431MetAsp: 1.431 ± 0.347
2.773MetGlu: 2.773 ± 0.41
0.894MetPhe: 0.894 ± 0.222
1.52MetGly: 1.52 ± 0.293
0.089MetHis: 0.089 ± 0.075
2.594MetIle: 2.594 ± 0.51
3.667MetLys: 3.667 ± 0.742
2.594MetLeu: 2.594 ± 0.515
0.537MetMet: 0.537 ± 0.234
1.789MetAsn: 1.789 ± 0.41
1.163MetPro: 1.163 ± 0.296
1.342MetGln: 1.342 ± 0.263
0.894MetArg: 0.894 ± 0.312
2.773MetSer: 2.773 ± 0.436
1.789MetThr: 1.789 ± 0.43
0.715MetVal: 0.715 ± 0.217
0.268MetTrp: 0.268 ± 0.143
1.163MetTyr: 1.163 ± 0.31
0.0MetXaa: 0.0 ± 0.0
Asn
2.415AsnAla: 2.415 ± 0.41
0.626AsnCys: 0.626 ± 0.219
1.699AsnAsp: 1.699 ± 0.46
4.025AsnGlu: 4.025 ± 0.693
1.073AsnPhe: 1.073 ± 0.322
4.204AsnGly: 4.204 ± 0.613
0.984AsnHis: 0.984 ± 0.283
2.773AsnIle: 2.773 ± 0.569
3.309AsnLys: 3.309 ± 0.5
3.935AsnLeu: 3.935 ± 0.571
1.252AsnMet: 1.252 ± 0.461
2.057AsnAsn: 2.057 ± 0.557
2.325AsnPro: 2.325 ± 0.515
1.431AsnGln: 1.431 ± 0.315
4.293AsnArg: 4.293 ± 0.778
2.683AsnSer: 2.683 ± 0.549
2.146AsnThr: 2.146 ± 0.408
2.415AsnVal: 2.415 ± 0.462
0.626AsnTrp: 0.626 ± 0.192
1.52AsnTyr: 1.52 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
2.236ProAla: 2.236 ± 0.358
0.179ProCys: 0.179 ± 0.133
1.61ProAsp: 1.61 ± 0.271
2.773ProGlu: 2.773 ± 0.625
1.252ProPhe: 1.252 ± 0.346
2.325ProGly: 2.325 ± 0.437
0.715ProHis: 0.715 ± 0.263
2.683ProIle: 2.683 ± 0.389
3.041ProLys: 3.041 ± 0.573
2.415ProLeu: 2.415 ± 0.532
0.894ProMet: 0.894 ± 0.263
1.789ProAsn: 1.789 ± 0.37
1.163ProPro: 1.163 ± 0.262
0.894ProGln: 0.894 ± 0.271
1.073ProArg: 1.073 ± 0.282
3.041ProSer: 3.041 ± 0.661
1.431ProThr: 1.431 ± 0.302
2.146ProVal: 2.146 ± 0.561
0.447ProTrp: 0.447 ± 0.192
1.163ProTyr: 1.163 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
3.488GlnAla: 3.488 ± 0.518
0.089GlnCys: 0.089 ± 0.09
1.878GlnAsp: 1.878 ± 0.328
3.577GlnGlu: 3.577 ± 0.613
1.699GlnPhe: 1.699 ± 0.3
2.683GlnGly: 2.683 ± 0.583
1.163GlnHis: 1.163 ± 0.279
3.22GlnIle: 3.22 ± 0.72
2.683GlnLys: 2.683 ± 0.436
4.204GlnLeu: 4.204 ± 0.736
2.057GlnMet: 2.057 ± 0.432
1.61GlnAsn: 1.61 ± 0.289
1.52GlnPro: 1.52 ± 0.449
1.789GlnGln: 1.789 ± 0.428
2.504GlnArg: 2.504 ± 0.513
2.146GlnSer: 2.146 ± 0.372
2.594GlnThr: 2.594 ± 0.466
1.789GlnVal: 1.789 ± 0.388
0.537GlnTrp: 0.537 ± 0.242
1.61GlnTyr: 1.61 ± 0.375
0.0GlnXaa: 0.0 ± 0.0
Arg
2.773ArgAla: 2.773 ± 0.42
0.626ArgCys: 0.626 ± 0.215
3.13ArgAsp: 3.13 ± 0.592
4.025ArgGlu: 4.025 ± 0.77
1.878ArgPhe: 1.878 ± 0.414
3.041ArgGly: 3.041 ± 0.567
0.984ArgHis: 0.984 ± 0.253
3.041ArgIle: 3.041 ± 0.589
6.439ArgLys: 6.439 ± 0.974
4.204ArgLeu: 4.204 ± 0.702
1.163ArgMet: 1.163 ± 0.366
2.057ArgAsn: 2.057 ± 0.404
1.431ArgPro: 1.431 ± 0.397
2.951ArgGln: 2.951 ± 0.47
2.862ArgArg: 2.862 ± 0.519
2.236ArgSer: 2.236 ± 0.466
2.236ArgThr: 2.236 ± 0.432
2.594ArgVal: 2.594 ± 0.384
0.894ArgTrp: 0.894 ± 0.273
1.878ArgTyr: 1.878 ± 0.339
0.0ArgXaa: 0.0 ± 0.0
Ser
5.992SerAla: 5.992 ± 1.133
0.358SerCys: 0.358 ± 0.169
4.204SerAsp: 4.204 ± 0.53
4.83SerGlu: 4.83 ± 0.708
2.951SerPhe: 2.951 ± 0.465
4.561SerGly: 4.561 ± 0.708
1.342SerHis: 1.342 ± 0.442
5.098SerIle: 5.098 ± 0.835
3.935SerLys: 3.935 ± 0.723
4.919SerLeu: 4.919 ± 0.668
1.789SerMet: 1.789 ± 0.372
1.968SerAsn: 1.968 ± 0.306
1.968SerPro: 1.968 ± 0.379
2.504SerGln: 2.504 ± 0.56
2.862SerArg: 2.862 ± 0.489
4.293SerSer: 4.293 ± 0.762
3.13SerThr: 3.13 ± 0.502
4.382SerVal: 4.382 ± 0.887
0.447SerTrp: 0.447 ± 0.158
2.236SerTyr: 2.236 ± 0.376
0.0SerXaa: 0.0 ± 0.0
Thr
3.846ThrAla: 3.846 ± 0.731
0.626ThrCys: 0.626 ± 0.211
2.862ThrAsp: 2.862 ± 0.391
3.22ThrGlu: 3.22 ± 0.518
1.61ThrPhe: 1.61 ± 0.324
3.756ThrGly: 3.756 ± 0.566
0.894ThrHis: 0.894 ± 0.346
3.399ThrIle: 3.399 ± 0.631
4.382ThrLys: 4.382 ± 0.63
4.651ThrLeu: 4.651 ± 0.777
1.699ThrMet: 1.699 ± 0.387
2.057ThrAsn: 2.057 ± 0.414
2.951ThrPro: 2.951 ± 0.603
2.146ThrGln: 2.146 ± 0.351
2.325ThrArg: 2.325 ± 0.48
3.667ThrSer: 3.667 ± 0.546
2.951ThrThr: 2.951 ± 0.593
4.651ThrVal: 4.651 ± 0.597
0.358ThrTrp: 0.358 ± 0.174
2.504ThrTyr: 2.504 ± 0.528
0.0ThrXaa: 0.0 ± 0.0
Val
3.667ValAla: 3.667 ± 0.682
0.537ValCys: 0.537 ± 0.242
3.399ValAsp: 3.399 ± 0.616
4.83ValGlu: 4.83 ± 0.791
2.773ValPhe: 2.773 ± 0.538
2.951ValGly: 2.951 ± 0.678
0.626ValHis: 0.626 ± 0.351
3.399ValIle: 3.399 ± 0.461
5.098ValLys: 5.098 ± 0.675
4.293ValLeu: 4.293 ± 0.487
1.61ValMet: 1.61 ± 0.353
2.594ValAsn: 2.594 ± 0.573
1.878ValPro: 1.878 ± 0.413
1.968ValGln: 1.968 ± 0.434
3.13ValArg: 3.13 ± 0.521
5.187ValSer: 5.187 ± 0.651
4.382ValThr: 4.382 ± 0.567
3.667ValVal: 3.667 ± 0.549
1.878ValTrp: 1.878 ± 1.015
1.968ValTyr: 1.968 ± 0.392
0.0ValXaa: 0.0 ± 0.0
Trp
0.626TrpAla: 0.626 ± 0.214
0.089TrpCys: 0.089 ± 0.087
0.626TrpAsp: 0.626 ± 0.336
0.626TrpGlu: 0.626 ± 0.196
0.805TrpPhe: 0.805 ± 0.245
1.431TrpGly: 1.431 ± 0.347
0.626TrpHis: 0.626 ± 0.24
0.805TrpIle: 0.805 ± 0.224
1.431TrpLys: 1.431 ± 0.359
1.61TrpLeu: 1.61 ± 0.382
0.089TrpMet: 0.089 ± 0.09
1.968TrpAsn: 1.968 ± 1.186
0.447TrpPro: 0.447 ± 0.278
0.626TrpGln: 0.626 ± 0.234
0.268TrpArg: 0.268 ± 0.167
0.715TrpSer: 0.715 ± 0.201
0.537TrpThr: 0.537 ± 0.226
0.537TrpVal: 0.537 ± 0.188
0.179TrpTrp: 0.179 ± 0.125
0.626TrpTyr: 0.626 ± 0.225
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.431TyrAla: 1.431 ± 0.4
0.537TyrCys: 0.537 ± 0.27
3.309TyrAsp: 3.309 ± 0.549
2.504TyrGlu: 2.504 ± 0.493
0.984TyrPhe: 0.984 ± 0.302
2.415TyrGly: 2.415 ± 0.527
0.268TyrHis: 0.268 ± 0.146
2.594TyrIle: 2.594 ± 0.471
2.951TyrLys: 2.951 ± 0.424
3.041TyrLeu: 3.041 ± 0.548
0.805TyrMet: 0.805 ± 0.24
1.61TyrAsn: 1.61 ± 0.388
1.252TyrPro: 1.252 ± 0.364
1.789TyrGln: 1.789 ± 0.307
1.878TyrArg: 1.878 ± 0.414
1.789TyrSer: 1.789 ± 0.497
2.236TyrThr: 2.236 ± 0.427
2.594TyrVal: 2.594 ± 0.447
0.358TyrTrp: 0.358 ± 0.181
1.789TyrTyr: 1.789 ± 0.445
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (11182 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski