Methylococcaceae bacterium FWC3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; unclassified Methylococcaceae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2839 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q5L1H8|A0A4Q5L1H8_9GAMM Thioredoxin OS=Methylococcaceae bacterium FWC3 OX=2516328 GN=trxA PE=3 SV=1
MM1 pKa = 7.56QDD3 pKa = 3.99LTPVSPPVIFTSNAAGKK20 pKa = 9.95VSEE23 pKa = 5.15LIADD27 pKa = 4.14EE28 pKa = 4.72GNPDD32 pKa = 3.45LKK34 pKa = 11.06LRR36 pKa = 11.84VYY38 pKa = 10.86VSGGGCSGMQYY49 pKa = 10.56GFSFEE54 pKa = 4.55EE55 pKa = 5.58GIADD59 pKa = 4.83DD60 pKa = 4.25DD61 pKa = 4.46TTVEE65 pKa = 4.16TDD67 pKa = 3.3GVTLLVDD74 pKa = 3.93SASLQYY80 pKa = 10.95LAGAEE85 pKa = 3.66IDD87 pKa = 3.86YY88 pKa = 11.32QEE90 pKa = 4.36GLEE93 pKa = 4.1GSRR96 pKa = 11.84FVIKK100 pKa = 10.68NPNASASCSCGSSFSVDD117 pKa = 3.01GGASCASTQQ126 pKa = 3.41

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5L2R4|A0A4Q5L2R4_9GAMM tRNA modification GTPase MnmE OS=Methylococcaceae bacterium FWC3 OX=2516328 GN=mnmE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 8.35IKK11 pKa = 10.26RR12 pKa = 11.84VRR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.04TPGGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.42GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2839

0

2839

968889

24

2559

341.3

37.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.377 ± 0.052

1.083 ± 0.015

5.584 ± 0.031

5.885 ± 0.037

3.808 ± 0.029

8.159 ± 0.043

2.272 ± 0.022

4.783 ± 0.034

2.845 ± 0.036

11.021 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.02

2.601 ± 0.031

5.348 ± 0.033

3.482 ± 0.025

7.728 ± 0.049

5.495 ± 0.03

5.156 ± 0.036

7.318 ± 0.036

1.489 ± 0.019

2.507 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski