Puma concolor (Mountain lion)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P6HL58|A0A6P6HL58_PUMCO Isoform of A0A6P6HKZ6 contactin-associated protein-like 4 isoform X2 OS=Puma concolor OX=9696 GN=CNTNAP4 PE=3 SV=1
MM1 pKa = 7.22EE2 pKa = 5.09AVVLIPYY9 pKa = 9.99VLGLLLLPLLAVVLCVRR26 pKa = 11.84CRR28 pKa = 11.84EE29 pKa = 4.27LPGSYY34 pKa = 10.75DD35 pKa = 3.3NTASDD40 pKa = 3.94SLAPSSIVIKK50 pKa = 10.5RR51 pKa = 11.84PPTLATWTPATSYY64 pKa = 10.76PPVTSYY70 pKa = 11.28PPLSQPDD77 pKa = 4.23LLPIPRR83 pKa = 11.84SPQPPGGSHH92 pKa = 6.82RR93 pKa = 11.84MPSSRR98 pKa = 11.84QDD100 pKa = 2.92SDD102 pKa = 3.47GANSVASYY110 pKa = 10.11EE111 pKa = 4.13NEE113 pKa = 4.24GASGAPGALVAGRR126 pKa = 11.84LGPGLGPADD135 pKa = 3.58RR136 pKa = 11.84CVVTSEE142 pKa = 4.45PACEE146 pKa = 5.39DD147 pKa = 3.57DD148 pKa = 6.66DD149 pKa = 4.6EE150 pKa = 6.88DD151 pKa = 5.49EE152 pKa = 4.94EE153 pKa = 4.44EE154 pKa = 4.91DD155 pKa = 4.15YY156 pKa = 11.46PNEE159 pKa = 4.39GYY161 pKa = 11.04LEE163 pKa = 4.18VLPDD167 pKa = 3.64STPTTGTAVPPAPAPSNPGLRR188 pKa = 11.84DD189 pKa = 3.14SAFSMEE195 pKa = 4.23SGEE198 pKa = 4.99DD199 pKa = 3.63YY200 pKa = 11.66VNVPEE205 pKa = 4.61SEE207 pKa = 4.19EE208 pKa = 4.19SADD211 pKa = 3.6VSLDD215 pKa = 3.29GSRR218 pKa = 11.84EE219 pKa = 3.96YY220 pKa = 11.72VNVSQEE226 pKa = 4.24LPPVARR232 pKa = 11.84TEE234 pKa = 3.93PAILSSQNDD243 pKa = 3.92DD244 pKa = 4.02EE245 pKa = 5.46EE246 pKa = 5.21EE247 pKa = 4.2GAPDD251 pKa = 3.75YY252 pKa = 11.56EE253 pKa = 4.31NLQGLNN259 pKa = 3.55

Molecular weight: 27.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P6HM51|A0A6P6HM51_PUMCO Thioredoxin-like protein OS=Puma concolor OX=9696 GN=TXNL4B PE=3 SV=1
MM1 pKa = 7.51GPGSGPNLRR10 pKa = 11.84AGVLLPSGNGPPNPRR25 pKa = 11.84PVGLGPGPSPNLRR38 pKa = 11.84SGFVGTNPAPRR49 pKa = 11.84SGMFPGPGLGPNPRR63 pKa = 11.84ANSLGPGLGPNPRR76 pKa = 11.84AGGLGPGPNLDD87 pKa = 3.45TRR89 pKa = 11.84AGGLLGTGSGLNLRR103 pKa = 11.84MAGPQGLDD111 pKa = 3.09LAPILRR117 pKa = 11.84AAGLLGANSAAFSQASGNMGTSPSSMARR145 pKa = 11.84VPGPMGPNSGPGSRR159 pKa = 11.84GIGLPGPNPSPMSRR173 pKa = 11.84APGPIGPNSAHH184 pKa = 7.19FSRR187 pKa = 11.84PAGPMGVNASPFPRR201 pKa = 11.84GTGSGGLNPAAFSQSSGTLASNPATFQRR229 pKa = 11.84SAGLQGSSPAIFPRR243 pKa = 11.84TSGPLGPNPANFPRR257 pKa = 11.84ASGLQGPSPAAFPRR271 pKa = 11.84STGPLGPGQVTFPRR285 pKa = 11.84SAPGPLGSSPAGPVGINPAPFARR308 pKa = 11.84PTGTLGLNPASFPRR322 pKa = 11.84MNGPVSKK329 pKa = 9.32TLVPFPRR336 pKa = 11.84VGNLPGTNPAAFPRR350 pKa = 11.84PGGPMAAMYY359 pKa = 9.22PNGMLPPP366 pKa = 4.73

Molecular weight: 35.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17614

5222

22836

13162948

31

34365

576.4

64.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.857 ± 0.016

2.194 ± 0.015

4.837 ± 0.011

7.114 ± 0.023

3.691 ± 0.013

6.517 ± 0.023

2.57 ± 0.01

4.481 ± 0.016

5.812 ± 0.025

9.887 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.008

3.659 ± 0.012

6.174 ± 0.022

4.699 ± 0.016

5.664 ± 0.017

8.312 ± 0.025

5.35 ± 0.018

6.117 ± 0.018

1.194 ± 0.006

2.675 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski