Thermus virus P23-45 (Thermus thermophilus phage P23-45)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Oshimavirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7XX23|A7XX23_BP234 Integrase OS=Thermus virus P23-45 OX=466051 GN=P23p5 PE=3 SV=1
MM1 pKa = 7.49TPMKK5 pKa = 10.28EE6 pKa = 3.91AFQGILPLVPKK17 pKa = 10.19RR18 pKa = 11.84CYY20 pKa = 10.57LAMGYY25 pKa = 10.86ALDD28 pKa = 4.42EE29 pKa = 4.69EE30 pKa = 5.25GNTYY34 pKa = 10.22IFAEE38 pKa = 4.35FDD40 pKa = 3.24DD41 pKa = 6.04GYY43 pKa = 11.15DD44 pKa = 3.24GFYY47 pKa = 10.73LRR49 pKa = 11.84EE50 pKa = 4.04EE51 pKa = 4.56DD52 pKa = 4.98SPDD55 pKa = 3.07GLARR59 pKa = 11.84LVNALRR65 pKa = 11.84TFMASYY71 pKa = 10.35EE72 pKa = 4.34SYY74 pKa = 11.32DD75 pKa = 3.46RR76 pKa = 11.84AII78 pKa = 3.99

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7XX82|A7XX82_BP234 Uncharacterized protein OS=Thermus virus P23-45 OX=466051 GN=P23p54 PE=4 SV=1
MM1 pKa = 7.55PHH3 pKa = 6.41LTDD6 pKa = 3.38STPWLTASLTRR17 pKa = 11.84TRR19 pKa = 11.84VEE21 pKa = 3.7RR22 pKa = 11.84RR23 pKa = 11.84YY24 pKa = 7.93GTPYY28 pKa = 10.28HH29 pKa = 6.58VIAVSRR35 pKa = 11.84DD36 pKa = 3.06LAKK39 pKa = 10.51RR40 pKa = 11.84IPFGTEE46 pKa = 3.03VRR48 pKa = 11.84LVCGDD53 pKa = 3.47RR54 pKa = 11.84VIYY57 pKa = 10.45GLVEE61 pKa = 4.33DD62 pKa = 4.53LMHH65 pKa = 6.62RR66 pKa = 11.84RR67 pKa = 11.84WRR69 pKa = 11.84SRR71 pKa = 11.84ADD73 pKa = 2.48IWFPSLHH80 pKa = 4.9QAKK83 pKa = 8.39TFGVCRR89 pKa = 11.84GYY91 pKa = 11.33LEE93 pKa = 3.89VLKK96 pKa = 9.84PVRR99 pKa = 11.84VIKK102 pKa = 10.27FKK104 pKa = 10.7RR105 pKa = 11.84EE106 pKa = 3.65RR107 pKa = 11.84YY108 pKa = 9.28GPEE111 pKa = 3.42ARR113 pKa = 11.84LQRR116 pKa = 3.97

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

117

0

117

27048

30

5002

231.2

25.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.646 ± 0.567

0.403 ± 0.079

5.231 ± 0.212

7.542 ± 0.446

4.111 ± 0.242

6.917 ± 0.226

1.671 ± 0.202

3.608 ± 0.277

5.072 ± 0.32

11.48 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.923 ± 0.112

3.22 ± 0.149

5.198 ± 0.296

3.483 ± 0.252

6.544 ± 0.256

5.28 ± 0.231

4.921 ± 0.243

8.178 ± 0.226

1.468 ± 0.216

4.104 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski