Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) (Pythium ultimum)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Pythiales; Pythiaceae; Globisporangium; Globisporangium ultimum

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K3X983|K3X983_GLOUD AB hydrolase-1 domain-containing protein OS=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) OX=431595 PE=4 SV=1
MM1 pKa = 7.77ANGKK5 pKa = 9.61LDD7 pKa = 4.17LFTVSDD13 pKa = 3.71SGVRR17 pKa = 11.84EE18 pKa = 3.96YY19 pKa = 11.45EE20 pKa = 4.0NLQGGQKK27 pKa = 9.21MLVEE31 pKa = 4.7FEE33 pKa = 4.48YY34 pKa = 10.88GSHH37 pKa = 6.77GSGISPSTFSDD48 pKa = 3.94EE49 pKa = 4.3SWCGHH54 pKa = 6.23EE55 pKa = 5.11IIASLVTMDD64 pKa = 4.26GVVKK68 pKa = 10.38NVNVMSIYY76 pKa = 10.66SLPPLDD82 pKa = 5.39FEE84 pKa = 6.24DD85 pKa = 4.12LAEE88 pKa = 4.57LQTMAPSLEE97 pKa = 4.35YY98 pKa = 10.91VDD100 pKa = 5.83DD101 pKa = 4.67FYY103 pKa = 11.63EE104 pKa = 4.75SEE106 pKa = 4.15VDD108 pKa = 4.55DD109 pKa = 4.51VSLAA113 pKa = 3.44

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K3WN77|K3WN77_GLOUD Uncharacterized protein OS=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) OX=431595 PE=4 SV=1
HHH2 pKa = 7.31GHHH5 pKa = 6.37VGHHH9 pKa = 7.02GRR11 pKa = 11.84RR12 pKa = 11.84LRR14 pKa = 11.84RR15 pKa = 11.84LHHH18 pKa = 6.53SHHH21 pKa = 6.17HHH23 pKa = 4.42HHH25 pKa = 5.83FFFVRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84LRR34 pKa = 11.84VHHH37 pKa = 6.44HHH39 pKa = 3.34TGRR42 pKa = 11.84ARR44 pKa = 11.84LLRR47 pKa = 11.84LVLALVLVLARR58 pKa = 11.84LLAGLASSARR68 pKa = 11.84HHH70 pKa = 3.62HHH72 pKa = 5.4FRR74 pKa = 11.84SRR76 pKa = 11.84HHH78 pKa = 4.9GRR80 pKa = 11.84HHH82 pKa = 4.51RR83 pKa = 11.84HHH85 pKa = 5.15RR86 pKa = 11.84GRR88 pKa = 11.84HHH90 pKa = 3.71HHH92 pKa = 5.44KKK94 pKa = 10.36GRR96 pKa = 11.84SRR98 pKa = 11.84LLHHH102 pKa = 5.8LEEE105 pKa = 4.13HH106 pKa = 6.96HH107 pKa = 6.48LAHHH111 pKa = 6.74FHHH114 pKa = 7.36LRR116 pKa = 11.84FHHH119 pKa = 7.22FFAHHH124 pKa = 5.39HHH126 pKa = 5.57RR127 pKa = 11.84RR128 pKa = 11.84LFRR131 pKa = 11.84HHH133 pKa = 5.51RR134 pKa = 11.84HHH136 pKa = 5.58HHH138 pKa = 4.5GHHH141 pKa = 6.11VGHHH145 pKa = 7.02GRR147 pKa = 11.84RR148 pKa = 11.84LRR150 pKa = 11.84RR151 pKa = 11.84LHHH154 pKa = 6.53SHHH157 pKa = 6.17HHH159 pKa = 4.42HHH161 pKa = 5.83FFFVRR166 pKa = 11.84RR167 pKa = 11.84RR168 pKa = 11.84LRR170 pKa = 11.84VHHH173 pKa = 6.52HHH175 pKa = 3.84

Molecular weight: 21.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15121

32

15153

6584968

28

8614

434.6

48.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.102 ± 0.021

1.603 ± 0.01

5.675 ± 0.015

6.268 ± 0.02

3.989 ± 0.013

5.904 ± 0.022

2.598 ± 0.01

4.583 ± 0.014

5.296 ± 0.02

9.294 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.008

3.745 ± 0.01

4.471 ± 0.016

4.305 ± 0.016

5.774 ± 0.015

8.237 ± 0.021

5.787 ± 0.015

6.977 ± 0.019

1.179 ± 0.007

2.723 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski