Bacillus phage Harambe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Harambevirus; Bacillus virus Harambe

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JSB7|A0A1W6JSB7_9CAUD Tail knob protein OS=Bacillus phage Harambe OX=1981931 GN=HARAMBE_20 PE=4 SV=1
MM1 pKa = 7.62SEE3 pKa = 4.49RR4 pKa = 11.84FIDD7 pKa = 4.34SYY9 pKa = 9.52TLIYY13 pKa = 9.1ITEE16 pKa = 4.43DD17 pKa = 3.17EE18 pKa = 4.23SGKK21 pKa = 10.51RR22 pKa = 11.84FDD24 pKa = 6.23CILEE28 pKa = 4.1NQTQEE33 pKa = 4.01DD34 pKa = 4.52CEE36 pKa = 4.27IIYY39 pKa = 10.82GNIIDD44 pKa = 6.11KK45 pKa = 10.72IIVWNMILDD54 pKa = 3.69MM55 pKa = 5.45

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JSC9|A0A1W6JSC9_9CAUD Upper collar protein OS=Bacillus phage Harambe OX=1981931 GN=HARAMBE_21 PE=4 SV=1
MM1 pKa = 7.36NKK3 pKa = 9.77YY4 pKa = 9.84KK5 pKa = 10.75RR6 pKa = 11.84LAEE9 pKa = 4.54RR10 pKa = 11.84INVRR14 pKa = 11.84WNGSKK19 pKa = 10.71CSTGWAHH26 pKa = 7.43DD27 pKa = 4.23MIDD30 pKa = 4.33AITILMMKK38 pKa = 8.78EE39 pKa = 3.61QNKK42 pKa = 10.0

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

6492

42

728

196.7

22.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.96 ± 0.476

0.832 ± 0.191

6.346 ± 0.252

8.133 ± 0.665

4.375 ± 0.274

6.377 ± 0.519

1.848 ± 0.212

6.947 ± 0.392

8.641 ± 0.408

6.839 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.127 ± 0.255

6.778 ± 0.371

2.85 ± 0.253

3.62 ± 0.311

4.113 ± 0.298

5.314 ± 0.232

6.916 ± 0.496

6.146 ± 0.457

1.14 ± 0.14

4.698 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski