Amino acid dipepetide frequency for Ruminiclostridium sufflavum DSM 19573

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.965AlaAla: 6.965 ± 0.115	0.963AlaCys: 0.963 ± 0.03	4.131AlaAsp: 4.131 ± 0.067	4.952AlaGlu: 4.952 ± 0.079	3.131AlaPhe: 3.131 ± 0.057	5.993AlaGly: 5.993 ± 0.129	0.879AlaHis: 0.879 ± 0.031	5.589AlaIle: 5.589 ± 0.073	4.615AlaLys: 4.615 ± 0.076	6.619AlaLeu: 6.619 ± 0.083	1.853AlaMet: 1.853 ± 0.04	2.859AlaAsn: 2.859 ± 0.054	1.887AlaPro: 1.887 ± 0.101	1.974AlaGln: 1.974 ± 0.045	2.549AlaArg: 2.549 ± 0.045	4.304AlaSer: 4.304 ± 0.07	2.812AlaThr: 2.812 ± 0.063	6.36AlaVal: 6.36 ± 0.092	0.538AlaTrp: 0.538 ± 0.024	2.688AlaTyr: 2.688 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.861CysAla: 0.861 ± 0.028	0.241CysCys: 0.241 ± 0.017	0.729CysAsp: 0.729 ± 0.027	0.876CysGlu: 0.876 ± 0.026	0.578CysPhe: 0.578 ± 0.024	1.292CysGly: 1.292 ± 0.032	0.251CysHis: 0.251 ± 0.015	1.196CysIle: 1.196 ± 0.033	0.947CysLys: 0.947 ± 0.029	1.066CysLeu: 1.066 ± 0.029	0.373CysMet: 0.373 ± 0.018	0.643CysAsn: 0.643 ± 0.023	0.536CysPro: 0.536 ± 0.022	0.296CysGln: 0.296 ± 0.017	0.606CysArg: 0.606 ± 0.023	1.025CysSer: 1.025 ± 0.034	0.684CysThr: 0.684 ± 0.025	0.757CysVal: 0.757 ± 0.025	0.12CysTrp: 0.12 ± 0.012	0.52CysTyr: 0.52 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.538AspAla: 3.538 ± 0.066	0.785AspCys: 0.785 ± 0.03	2.445AspAsp: 2.445 ± 0.049	4.071AspGlu: 4.071 ± 0.065	2.736AspPhe: 2.736 ± 0.047	3.954AspGly: 3.954 ± 0.085	0.521AspHis: 0.521 ± 0.021	5.87AspIle: 5.87 ± 0.077	4.558AspLys: 4.558 ± 0.062	4.174AspLeu: 4.174 ± 0.06	1.668AspMet: 1.668 ± 0.035	3.068AspAsn: 3.068 ± 0.057	1.358AspPro: 1.358 ± 0.037	0.789AspGln: 0.789 ± 0.023	2.171AspArg: 2.171 ± 0.045	3.888AspSer: 3.888 ± 0.062	3.201AspThr: 3.201 ± 0.057	3.245AspVal: 3.245 ± 0.06	0.543AspTrp: 0.543 ± 0.018	2.595AspTyr: 2.595 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	5.233GluAla: 5.233 ± 0.067	0.705GluCys: 0.705 ± 0.022	3.586GluAsp: 3.586 ± 0.056	5.357GluGlu: 5.357 ± 0.077	2.614GluPhe: 2.614 ± 0.048	4.031GluGly: 4.031 ± 0.066	1.015GluHis: 1.015 ± 0.031	6.144GluIle: 6.144 ± 0.083	6.263GluLys: 6.263 ± 0.076	6.674GluLeu: 6.674 ± 0.083	1.912GluMet: 1.912 ± 0.04	4.194GluAsn: 4.194 ± 0.061	1.697GluPro: 1.697 ± 0.039	2.512GluGln: 2.512 ± 0.058	2.882GluArg: 2.882 ± 0.055	3.402GluSer: 3.402 ± 0.057	3.066GluThr: 3.066 ± 0.051	4.171GluVal: 4.171 ± 0.065	0.606GluTrp: 0.606 ± 0.022	3.175GluTyr: 3.175 ± 0.067	0.0GluXaa: 0.0 ± 0.0
Phe	2.891PheAla: 2.891 ± 0.057	0.698PheCys: 0.698 ± 0.022	2.755PheAsp: 2.755 ± 0.051	2.912PheGlu: 2.912 ± 0.057	1.892PhePhe: 1.892 ± 0.047	2.929PheGly: 2.929 ± 0.05	0.555PheHis: 0.555 ± 0.024	3.76PheIle: 3.76 ± 0.069	3.047PheLys: 3.047 ± 0.05	3.571PheLeu: 3.571 ± 0.065	1.063PheMet: 1.063 ± 0.031	2.377PheAsn: 2.377 ± 0.044	1.3PhePro: 1.3 ± 0.031	0.95PheGln: 0.95 ± 0.024	1.466PheArg: 1.466 ± 0.033	3.304PheSer: 3.304 ± 0.047	2.297PheThr: 2.297 ± 0.047	2.651PheVal: 2.651 ± 0.054	0.373PheTrp: 0.373 ± 0.018	1.729PheTyr: 1.729 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.57GlyAla: 4.57 ± 0.088	1.072GlyCys: 1.072 ± 0.033	3.488GlyAsp: 3.488 ± 0.066	4.24GlyGlu: 4.24 ± 0.061	3.135GlyPhe: 3.135 ± 0.056	4.529GlyGly: 4.529 ± 0.077	1.049GlyHis: 1.049 ± 0.03	6.789GlyIle: 6.789 ± 0.078	5.342GlyLys: 5.342 ± 0.065	5.737GlyLeu: 5.737 ± 0.072	2.02GlyMet: 2.02 ± 0.041	3.496GlyAsn: 3.496 ± 0.083	1.548GlyPro: 1.548 ± 0.187	1.883GlyGln: 1.883 ± 0.045	2.831GlyArg: 2.831 ± 0.06	4.458GlySer: 4.458 ± 0.07	4.167GlyThr: 4.167 ± 0.101	4.318GlyVal: 4.318 ± 0.066	0.628GlyTrp: 0.628 ± 0.026	3.148GlyTyr: 3.148 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	0.821HisAla: 0.821 ± 0.029	0.255HisCys: 0.255 ± 0.015	0.732HisAsp: 0.732 ± 0.024	0.804HisGlu: 0.804 ± 0.026	0.671HisPhe: 0.671 ± 0.023	1.022HisGly: 1.022 ± 0.029	0.299HisHis: 0.299 ± 0.018	1.269HisIle: 1.269 ± 0.033	0.964HisLys: 0.964 ± 0.027	1.131HisLeu: 1.131 ± 0.034	0.364HisMet: 0.364 ± 0.018	0.738HisAsn: 0.738 ± 0.028	0.659HisPro: 0.659 ± 0.028	0.362HisGln: 0.362 ± 0.017	0.644HisArg: 0.644 ± 0.027	0.962HisSer: 0.962 ± 0.035	0.799HisThr: 0.799 ± 0.024	0.769HisVal: 0.769 ± 0.024	0.145HisTrp: 0.145 ± 0.01	0.597HisTyr: 0.597 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.143IleAla: 6.143 ± 0.085	1.316IleCys: 1.316 ± 0.032	5.273IleAsp: 5.273 ± 0.062	5.805IleGlu: 5.805 ± 0.077	3.573IlePhe: 3.573 ± 0.062	5.402IleGly: 5.402 ± 0.078	1.169IleHis: 1.169 ± 0.031	7.511IleIle: 7.511 ± 0.117	6.699IleLys: 6.699 ± 0.08	7.433IleLeu: 7.433 ± 0.087	2.046IleMet: 2.046 ± 0.046	4.996IleAsn: 4.996 ± 0.069	3.349IlePro: 3.349 ± 0.07	2.339IleGln: 2.339 ± 0.043	3.302IleArg: 3.302 ± 0.055	6.894IleSer: 6.894 ± 0.084	4.76IleThr: 4.76 ± 0.069	5.114IleVal: 5.114 ± 0.075	0.632IleTrp: 0.632 ± 0.027	3.149IleTyr: 3.149 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	5.892LysAla: 5.892 ± 0.08	0.821LysCys: 0.821 ± 0.029	4.346LysAsp: 4.346 ± 0.059	6.055LysGlu: 6.055 ± 0.081	2.34LysPhe: 2.34 ± 0.047	4.446LysGly: 4.446 ± 0.06	1.096LysHis: 1.096 ± 0.03	6.354LysIle: 6.354 ± 0.072	6.21LysLys: 6.21 ± 0.077	6.652LysLeu: 6.652 ± 0.093	2.0LysMet: 2.0 ± 0.045	4.694LysAsn: 4.694 ± 0.059	2.307LysPro: 2.307 ± 0.044	2.433LysGln: 2.433 ± 0.045	3.035LysArg: 3.035 ± 0.056	4.647LysSer: 4.647 ± 0.067	4.092LysThr: 4.092 ± 0.063	4.575LysVal: 4.575 ± 0.068	0.68LysTrp: 0.68 ± 0.026	3.592LysTyr: 3.592 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	6.028LeuAla: 6.028 ± 0.074	1.247LeuCys: 1.247 ± 0.03	4.842LeuAsp: 4.842 ± 0.07	5.968LeuGlu: 5.968 ± 0.082	3.814LeuPhe: 3.814 ± 0.062	5.629LeuGly: 5.629 ± 0.074	1.239LeuHis: 1.239 ± 0.039	6.969LeuIle: 6.969 ± 0.106	7.387LeuLys: 7.387 ± 0.071	8.235LeuLeu: 8.235 ± 0.098	2.278LeuMet: 2.278 ± 0.048	4.763LeuAsn: 4.763 ± 0.067	3.176LeuPro: 3.176 ± 0.062	2.512LeuGln: 2.512 ± 0.043	3.445LeuArg: 3.445 ± 0.06	6.939LeuSer: 6.939 ± 0.092	4.72LeuThr: 4.72 ± 0.066	5.167LeuVal: 5.167 ± 0.075	0.682LeuTrp: 0.682 ± 0.023	3.317LeuTyr: 3.317 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	1.905MetAla: 1.905 ± 0.042	0.296MetCys: 0.296 ± 0.015	1.492MetAsp: 1.492 ± 0.036	1.726MetGlu: 1.726 ± 0.044	0.949MetPhe: 0.949 ± 0.03	1.785MetGly: 1.785 ± 0.045	0.411MetHis: 0.411 ± 0.02	1.91MetIle: 1.91 ± 0.045	2.288MetLys: 2.288 ± 0.049	2.735MetLeu: 2.735 ± 0.05	0.671MetMet: 0.671 ± 0.024	1.428MetAsn: 1.428 ± 0.032	1.065MetPro: 1.065 ± 0.029	0.892MetGln: 0.892 ± 0.026	0.97MetArg: 0.97 ± 0.029	1.8MetSer: 1.8 ± 0.036	1.353MetThr: 1.353 ± 0.038	1.571MetVal: 1.571 ± 0.037	0.195MetTrp: 0.195 ± 0.012	0.881MetTyr: 0.881 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.417AsnAla: 3.417 ± 0.046	0.717AsnCys: 0.717 ± 0.024	2.599AsnAsp: 2.599 ± 0.043	3.577AsnGlu: 3.577 ± 0.059	1.956AsnPhe: 1.956 ± 0.044	3.886AsnGly: 3.886 ± 0.076	0.678AsnHis: 0.678 ± 0.024	5.477AsnIle: 5.477 ± 0.074	4.053AsnLys: 4.053 ± 0.063	4.173AsnLeu: 4.173 ± 0.065	1.459AsnMet: 1.459 ± 0.037	3.174AsnAsn: 3.174 ± 0.06	2.066AsnPro: 2.066 ± 0.047	1.519AsnGln: 1.519 ± 0.039	2.077AsnArg: 2.077 ± 0.04	3.818AsnSer: 3.818 ± 0.062	3.094AsnThr: 3.094 ± 0.066	3.156AsnVal: 3.156 ± 0.048	0.468AsnTrp: 0.468 ± 0.021	2.187AsnTyr: 2.187 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	2.53ProAla: 2.53 ± 0.117	0.398ProCys: 0.398 ± 0.017	2.144ProAsp: 2.144 ± 0.04	2.777ProGlu: 2.777 ± 0.056	1.477ProPhe: 1.477 ± 0.038	2.016ProGly: 2.016 ± 0.041	0.493ProHis: 0.493 ± 0.021	2.146ProIle: 2.146 ± 0.046	1.835ProLys: 1.835 ± 0.036	2.553ProLeu: 2.553 ± 0.044	0.747ProMet: 0.747 ± 0.027	1.332ProAsn: 1.332 ± 0.04	0.792ProPro: 0.792 ± 0.027	1.03ProGln: 1.03 ± 0.03	0.901ProArg: 0.901 ± 0.025	1.958ProSer: 1.958 ± 0.044	1.382ProThr: 1.382 ± 0.047	3.39ProVal: 3.39 ± 0.305	0.303ProTrp: 0.303 ± 0.017	1.328ProTyr: 1.328 ± 0.036	0.001ProXaa: 0.001 ± 0.001
Gln	2.153GlnAla: 2.153 ± 0.043	0.314GlnCys: 0.314 ± 0.018	1.347GlnAsp: 1.347 ± 0.03	1.988GlnGlu: 1.988 ± 0.04	1.044GlnPhe: 1.044 ± 0.028	1.695GlnGly: 1.695 ± 0.04	0.376GlnHis: 0.376 ± 0.016	2.398GlnIle: 2.398 ± 0.046	2.555GlnLys: 2.555 ± 0.051	2.696GlnLeu: 2.696 ± 0.049	0.817GlnMet: 0.817 ± 0.022	1.637GlnAsn: 1.637 ± 0.04	0.857GlnPro: 0.857 ± 0.025	1.057GlnGln: 1.057 ± 0.036	1.215GlnArg: 1.215 ± 0.036	1.769GlnSer: 1.769 ± 0.036	1.391GlnThr: 1.391 ± 0.037	1.638GlnVal: 1.638 ± 0.043	0.264GlnTrp: 0.264 ± 0.015	1.285GlnTyr: 1.285 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.383ArgAla: 2.383 ± 0.045	0.509ArgCys: 0.509 ± 0.024	2.005ArgAsp: 2.005 ± 0.04	2.939ArgGlu: 2.939 ± 0.051	1.729ArgPhe: 1.729 ± 0.043	2.151ArgGly: 2.151 ± 0.041	0.62ArgHis: 0.62 ± 0.027	3.549ArgIle: 3.549 ± 0.06	3.071ArgLys: 3.071 ± 0.053	3.981ArgLeu: 3.981 ± 0.072	1.157ArgMet: 1.157 ± 0.03	2.147ArgAsn: 2.147 ± 0.044	1.083ArgPro: 1.083 ± 0.029	1.372ArgGln: 1.372 ± 0.035	1.726ArgArg: 1.726 ± 0.043	1.961ArgSer: 1.961 ± 0.04	1.945ArgThr: 1.945 ± 0.041	2.438ArgVal: 2.438 ± 0.049	0.35ArgTrp: 0.35 ± 0.018	1.746ArgTyr: 1.746 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.582SerAla: 4.582 ± 0.078	0.907SerCys: 0.907 ± 0.026	3.917SerAsp: 3.917 ± 0.064	4.6SerGlu: 4.6 ± 0.064	3.306SerPhe: 3.306 ± 0.057	5.67SerGly: 5.67 ± 0.082	0.945SerHis: 0.945 ± 0.028	5.788SerIle: 5.788 ± 0.074	4.71SerLys: 4.71 ± 0.065	5.83SerLeu: 5.83 ± 0.085	1.73SerMet: 1.73 ± 0.045	3.286SerAsn: 3.286 ± 0.062	1.953SerPro: 1.953 ± 0.04	1.958SerGln: 1.958 ± 0.039	2.732SerArg: 2.732 ± 0.047	4.855SerSer: 4.855 ± 0.099	3.337SerThr: 3.337 ± 0.053	4.528SerVal: 4.528 ± 0.056	0.589SerTrp: 0.589 ± 0.025	2.654SerTyr: 2.654 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.55ThrAla: 4.55 ± 0.084	0.593ThrCys: 0.593 ± 0.021	3.013ThrAsp: 3.013 ± 0.069	3.387ThrGlu: 3.387 ± 0.065	2.096ThrPhe: 2.096 ± 0.051	4.848ThrGly: 4.848 ± 0.091	0.764ThrHis: 0.764 ± 0.021	4.172ThrIle: 4.172 ± 0.066	3.237ThrLys: 3.237 ± 0.061	4.454ThrLeu: 4.454 ± 0.072	1.122ThrMet: 1.122 ± 0.033	2.319ThrAsn: 2.319 ± 0.052	1.983ThrPro: 1.983 ± 0.091	1.363ThrGln: 1.363 ± 0.033	1.745ThrArg: 1.745 ± 0.039	3.295ThrSer: 3.295 ± 0.058	2.517ThrThr: 2.517 ± 0.07	4.215ThrVal: 4.215 ± 0.082	0.446ThrTrp: 0.446 ± 0.023	2.111ThrTyr: 2.111 ± 0.073	0.0ThrXaa: 0.0 ± 0.0
Val	4.222ValAla: 4.222 ± 0.101	1.007ValCys: 1.007 ± 0.025	3.389ValAsp: 3.389 ± 0.053	3.943ValGlu: 3.943 ± 0.068	3.09ValPhe: 3.09 ± 0.054	3.707ValGly: 3.707 ± 0.062	0.894ValHis: 0.894 ± 0.03	5.77ValIle: 5.77 ± 0.087	4.836ValLys: 4.836 ± 0.057	6.251ValLeu: 6.251 ± 0.076	1.749ValMet: 1.749 ± 0.041	3.512ValAsn: 3.512 ± 0.053	2.28ValPro: 2.28 ± 0.047	1.811ValGln: 1.811 ± 0.039	2.479ValArg: 2.479 ± 0.053	4.953ValSer: 4.953 ± 0.082	3.793ValThr: 3.793 ± 0.112	4.171ValVal: 4.171 ± 0.069	0.57ValTrp: 0.57 ± 0.032	2.526ValTyr: 2.526 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.543TrpAla: 0.543 ± 0.025	0.144TrpCys: 0.144 ± 0.01	0.524TrpAsp: 0.524 ± 0.025	0.558TrpGlu: 0.558 ± 0.024	0.387TrpPhe: 0.387 ± 0.018	0.644TrpGly: 0.644 ± 0.032	0.159TrpHis: 0.159 ± 0.012	0.601TrpIle: 0.601 ± 0.023	0.623TrpLys: 0.623 ± 0.023	0.777TrpLeu: 0.777 ± 0.023	0.261TrpMet: 0.261 ± 0.015	0.543TrpAsn: 0.543 ± 0.022	0.207TrpPro: 0.207 ± 0.015	0.327TrpGln: 0.327 ± 0.018	0.356TrpArg: 0.356 ± 0.016	0.58TrpSer: 0.58 ± 0.028	0.433TrpThr: 0.433 ± 0.021	0.481TrpVal: 0.481 ± 0.023	0.11TrpTrp: 0.11 ± 0.01	0.368TrpTyr: 0.368 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.433TyrAla: 2.433 ± 0.046	0.61TyrCys: 0.61 ± 0.022	2.42TyrAsp: 2.42 ± 0.086	2.706TyrGlu: 2.706 ± 0.056	2.006TyrPhe: 2.006 ± 0.042	2.815TyrGly: 2.815 ± 0.052	0.595TyrHis: 0.595 ± 0.025	3.646TyrIle: 3.646 ± 0.061	3.024TyrLys: 3.024 ± 0.049	3.616TyrLeu: 3.616 ± 0.064	1.034TyrMet: 1.034 ± 0.031	2.343TyrAsn: 2.343 ± 0.048	1.431TyrPro: 1.431 ± 0.036	1.115TyrGln: 1.115 ± 0.032	1.729TyrArg: 1.729 ± 0.037	3.066TyrSer: 3.066 ± 0.066	2.387TyrThr: 2.387 ± 0.063	2.236TyrVal: 2.236 ± 0.046	0.382TyrTrp: 0.382 ± 0.018	1.776TyrTyr: 1.776 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3674 proteins (1258753 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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