Amino acid dipepetide frequency for Mycobacterium phage Whouxphf

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.262AlaAla: 13.262 ± 1.492
1.21AlaCys: 1.21 ± 0.252
6.999AlaAsp: 6.999 ± 0.681
7.21AlaGlu: 7.21 ± 0.669
2.631AlaPhe: 2.631 ± 0.33
9.946AlaGly: 9.946 ± 1.017
2.473AlaHis: 2.473 ± 0.363
4.473AlaIle: 4.473 ± 0.67
4.157AlaLys: 4.157 ± 0.476
7.578AlaLeu: 7.578 ± 0.722
2.263AlaMet: 2.263 ± 0.341
2.842AlaAsn: 2.842 ± 0.434
4.736AlaPro: 4.736 ± 0.5
3.263AlaGln: 3.263 ± 0.457
7.262AlaArg: 7.262 ± 0.723
4.999AlaSer: 4.999 ± 0.467
6.578AlaThr: 6.578 ± 0.59
6.789AlaVal: 6.789 ± 0.562
2.263AlaTrp: 2.263 ± 0.396
2.105AlaTyr: 2.105 ± 0.378
0.0AlaXaa: 0.0 ± 0.0
Cys
1.0CysAla: 1.0 ± 0.269
0.105CysCys: 0.105 ± 0.075
1.263CysAsp: 1.263 ± 0.284
0.842CysGlu: 0.842 ± 0.197
0.211CysPhe: 0.211 ± 0.107
1.842CysGly: 1.842 ± 0.411
0.474CysHis: 0.474 ± 0.201
0.105CysIle: 0.105 ± 0.067
0.474CysLys: 0.474 ± 0.155
0.737CysLeu: 0.737 ± 0.264
0.263CysMet: 0.263 ± 0.116
0.316CysAsn: 0.316 ± 0.12
1.579CysPro: 1.579 ± 0.316
0.263CysGln: 0.263 ± 0.109
0.684CysArg: 0.684 ± 0.19
0.895CysSer: 0.895 ± 0.27
0.842CysThr: 0.842 ± 0.212
0.789CysVal: 0.789 ± 0.203
0.263CysTrp: 0.263 ± 0.11
0.158CysTyr: 0.158 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
6.578AspAla: 6.578 ± 0.559
0.947AspCys: 0.947 ± 0.203
4.578AspAsp: 4.578 ± 0.563
3.315AspGlu: 3.315 ± 0.395
1.421AspPhe: 1.421 ± 0.225
6.684AspGly: 6.684 ± 0.621
1.474AspHis: 1.474 ± 0.239
2.421AspIle: 2.421 ± 0.363
1.789AspLys: 1.789 ± 0.329
5.315AspLeu: 5.315 ± 0.586
1.263AspMet: 1.263 ± 0.331
1.526AspAsn: 1.526 ± 0.355
4.894AspPro: 4.894 ± 0.466
2.473AspGln: 2.473 ± 0.319
5.42AspArg: 5.42 ± 0.583
3.947AspSer: 3.947 ± 0.525
4.0AspThr: 4.0 ± 0.422
4.315AspVal: 4.315 ± 0.525
1.684AspTrp: 1.684 ± 0.311
1.789AspTyr: 1.789 ± 0.269
0.0AspXaa: 0.0 ± 0.0
Glu
6.157GluAla: 6.157 ± 0.531
0.789GluCys: 0.789 ± 0.227
3.421GluAsp: 3.421 ± 0.408
3.052GluGlu: 3.052 ± 0.432
2.158GluPhe: 2.158 ± 0.35
3.579GluGly: 3.579 ± 0.352
1.947GluHis: 1.947 ± 0.341
2.368GluIle: 2.368 ± 0.348
1.789GluLys: 1.789 ± 0.293
5.736GluLeu: 5.736 ± 0.625
1.368GluMet: 1.368 ± 0.236
2.21GluAsn: 2.21 ± 0.29
3.052GluPro: 3.052 ± 0.51
3.052GluGln: 3.052 ± 0.4
5.052GluArg: 5.052 ± 0.579
3.0GluSer: 3.0 ± 0.436
4.105GluThr: 4.105 ± 0.527
4.21GluVal: 4.21 ± 0.476
1.631GluTrp: 1.631 ± 0.336
1.631GluTyr: 1.631 ± 0.259
0.0GluXaa: 0.0 ± 0.0
Phe
3.052PheAla: 3.052 ± 0.372
0.263PheCys: 0.263 ± 0.117
2.316PheAsp: 2.316 ± 0.383
1.368PheGlu: 1.368 ± 0.271
0.895PhePhe: 0.895 ± 0.238
2.631PheGly: 2.631 ± 0.673
0.368PheHis: 0.368 ± 0.165
1.368PheIle: 1.368 ± 0.354
1.0PheLys: 1.0 ± 0.269
2.158PheLeu: 2.158 ± 0.294
0.632PheMet: 0.632 ± 0.183
1.158PheAsn: 1.158 ± 0.314
1.631PhePro: 1.631 ± 0.312
1.263PheGln: 1.263 ± 0.285
1.737PheArg: 1.737 ± 0.279
1.421PheSer: 1.421 ± 0.254
2.473PheThr: 2.473 ± 0.386
2.105PheVal: 2.105 ± 0.257
0.526PheTrp: 0.526 ± 0.128
0.895PheTyr: 0.895 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
7.947GlyAla: 7.947 ± 0.964
1.316GlyCys: 1.316 ± 0.312
5.473GlyAsp: 5.473 ± 0.427
4.631GlyGlu: 4.631 ± 0.497
2.737GlyPhe: 2.737 ± 0.507
10.894GlyGly: 10.894 ± 2.676
2.052GlyHis: 2.052 ± 0.288
3.684GlyIle: 3.684 ± 0.519
2.526GlyLys: 2.526 ± 0.347
6.21GlyLeu: 6.21 ± 0.661
2.473GlyMet: 2.473 ± 0.445
2.894GlyAsn: 2.894 ± 0.435
4.578GlyPro: 4.578 ± 0.61
2.684GlyGln: 2.684 ± 0.604
5.631GlyArg: 5.631 ± 0.548
6.105GlySer: 6.105 ± 0.887
6.42GlyThr: 6.42 ± 0.791
5.947GlyVal: 5.947 ± 0.625
2.579GlyTrp: 2.579 ± 0.339
1.947GlyTyr: 1.947 ± 0.35
0.0GlyXaa: 0.0 ± 0.0
His
2.052HisAla: 2.052 ± 0.317
0.526HisCys: 0.526 ± 0.185
1.263HisAsp: 1.263 ± 0.245
1.21HisGlu: 1.21 ± 0.231
0.632HisPhe: 0.632 ± 0.162
1.631HisGly: 1.631 ± 0.292
0.947HisHis: 0.947 ± 0.226
1.579HisIle: 1.579 ± 0.293
0.737HisLys: 0.737 ± 0.238
1.579HisLeu: 1.579 ± 0.311
0.579HisMet: 0.579 ± 0.19
1.053HisAsn: 1.053 ± 0.24
1.368HisPro: 1.368 ± 0.236
0.737HisGln: 0.737 ± 0.168
2.0HisArg: 2.0 ± 0.326
1.421HisSer: 1.421 ± 0.254
1.316HisThr: 1.316 ± 0.296
1.526HisVal: 1.526 ± 0.313
0.421HisTrp: 0.421 ± 0.127
0.842HisTyr: 0.842 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
5.105IleAla: 5.105 ± 0.532
0.474IleCys: 0.474 ± 0.171
3.894IleAsp: 3.894 ± 0.516
3.789IleGlu: 3.789 ± 0.402
0.842IlePhe: 0.842 ± 0.221
3.894IleGly: 3.894 ± 0.387
1.368IleHis: 1.368 ± 0.256
1.631IleIle: 1.631 ± 0.282
1.158IleLys: 1.158 ± 0.257
1.947IleLeu: 1.947 ± 0.33
0.263IleMet: 0.263 ± 0.121
1.737IleAsn: 1.737 ± 0.267
2.789IlePro: 2.789 ± 0.362
1.631IleGln: 1.631 ± 0.325
2.368IleArg: 2.368 ± 0.348
2.316IleSer: 2.316 ± 0.449
3.736IleThr: 3.736 ± 0.385
2.842IleVal: 2.842 ± 0.374
0.947IleTrp: 0.947 ± 0.212
0.737IleTyr: 0.737 ± 0.174
0.0IleXaa: 0.0 ± 0.0
Lys
3.789LysAla: 3.789 ± 0.458
0.579LysCys: 0.579 ± 0.182
1.631LysAsp: 1.631 ± 0.262
1.684LysGlu: 1.684 ± 0.286
1.368LysPhe: 1.368 ± 0.238
2.526LysGly: 2.526 ± 0.341
1.316LysHis: 1.316 ± 0.341
1.0LysIle: 1.0 ± 0.185
1.684LysLys: 1.684 ± 0.44
2.316LysLeu: 2.316 ± 0.439
0.579LysMet: 0.579 ± 0.169
1.0LysAsn: 1.0 ± 0.227
2.737LysPro: 2.737 ± 0.392
1.631LysGln: 1.631 ± 0.272
2.052LysArg: 2.052 ± 0.305
2.158LysSer: 2.158 ± 0.308
2.21LysThr: 2.21 ± 0.368
2.737LysVal: 2.737 ± 0.376
0.895LysTrp: 0.895 ± 0.266
0.632LysTyr: 0.632 ± 0.184
0.0LysXaa: 0.0 ± 0.0
Leu
8.262LeuAla: 8.262 ± 0.787
0.737LeuCys: 0.737 ± 0.207
4.578LeuAsp: 4.578 ± 0.503
4.368LeuGlu: 4.368 ± 0.508
2.473LeuPhe: 2.473 ± 0.297
4.526LeuGly: 4.526 ± 0.469
1.0LeuHis: 1.0 ± 0.227
3.368LeuIle: 3.368 ± 0.369
2.421LeuLys: 2.421 ± 0.374
5.368LeuLeu: 5.368 ± 0.518
1.684LeuMet: 1.684 ± 0.351
2.789LeuAsn: 2.789 ± 0.352
4.894LeuPro: 4.894 ± 0.639
2.789LeuGln: 2.789 ± 0.432
4.684LeuArg: 4.684 ± 0.587
4.999LeuSer: 4.999 ± 0.505
5.052LeuThr: 5.052 ± 0.527
4.368LeuVal: 4.368 ± 0.459
1.316LeuTrp: 1.316 ± 0.272
2.158LeuTyr: 2.158 ± 0.408
0.0LeuXaa: 0.0 ± 0.0
Met
2.052MetAla: 2.052 ± 0.32
0.211MetCys: 0.211 ± 0.166
1.105MetAsp: 1.105 ± 0.256
1.0MetGlu: 1.0 ± 0.205
0.684MetPhe: 0.684 ± 0.225
1.947MetGly: 1.947 ± 0.247
0.158MetHis: 0.158 ± 0.088
0.789MetIle: 0.789 ± 0.186
0.632MetLys: 0.632 ± 0.199
2.052MetLeu: 2.052 ± 0.309
0.421MetMet: 0.421 ± 0.192
0.947MetAsn: 0.947 ± 0.218
1.158MetPro: 1.158 ± 0.261
0.579MetGln: 0.579 ± 0.16
1.842MetArg: 1.842 ± 0.336
2.842MetSer: 2.842 ± 0.387
2.526MetThr: 2.526 ± 0.321
1.316MetVal: 1.316 ± 0.304
0.316MetTrp: 0.316 ± 0.114
0.263MetTyr: 0.263 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
3.473AsnAla: 3.473 ± 0.361
0.211AsnCys: 0.211 ± 0.109
1.789AsnAsp: 1.789 ± 0.295
1.579AsnGlu: 1.579 ± 0.322
0.947AsnPhe: 0.947 ± 0.253
3.894AsnGly: 3.894 ± 0.456
1.0AsnHis: 1.0 ± 0.19
1.474AsnIle: 1.474 ± 0.437
1.21AsnLys: 1.21 ± 0.256
2.316AsnLeu: 2.316 ± 0.334
0.789AsnMet: 0.789 ± 0.16
1.737AsnAsn: 1.737 ± 0.298
2.579AsnPro: 2.579 ± 0.387
1.105AsnGln: 1.105 ± 0.293
1.947AsnArg: 1.947 ± 0.33
1.631AsnSer: 1.631 ± 0.296
1.947AsnThr: 1.947 ± 0.274
2.21AsnVal: 2.21 ± 0.379
0.684AsnTrp: 0.684 ± 0.19
0.947AsnTyr: 0.947 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
5.263ProAla: 5.263 ± 0.573
0.895ProCys: 0.895 ± 0.218
4.105ProAsp: 4.105 ± 0.487
4.631ProGlu: 4.631 ± 0.446
1.789ProPhe: 1.789 ± 0.356
6.578ProGly: 6.578 ± 0.635
1.579ProHis: 1.579 ± 0.317
1.947ProIle: 1.947 ± 0.313
2.421ProLys: 2.421 ± 0.35
4.473ProLeu: 4.473 ± 0.485
1.737ProMet: 1.737 ± 0.351
2.316ProAsn: 2.316 ± 0.345
4.263ProPro: 4.263 ± 0.585
2.473ProGln: 2.473 ± 0.387
3.263ProArg: 3.263 ± 0.481
3.842ProSer: 3.842 ± 0.472
3.315ProThr: 3.315 ± 0.459
4.526ProVal: 4.526 ± 0.511
1.158ProTrp: 1.158 ± 0.248
1.421ProTyr: 1.421 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
4.105GlnAla: 4.105 ± 0.456
0.474GlnCys: 0.474 ± 0.188
1.579GlnAsp: 1.579 ± 0.267
1.421GlnGlu: 1.421 ± 0.282
1.053GlnPhe: 1.053 ± 0.223
2.579GlnGly: 2.579 ± 0.477
0.895GlnHis: 0.895 ± 0.222
1.895GlnIle: 1.895 ± 0.319
1.421GlnLys: 1.421 ± 0.217
3.105GlnLeu: 3.105 ± 0.394
0.737GlnMet: 0.737 ± 0.215
0.947GlnAsn: 0.947 ± 0.257
2.421GlnPro: 2.421 ± 0.381
1.368GlnGln: 1.368 ± 0.266
2.579GlnArg: 2.579 ± 0.357
2.421GlnSer: 2.421 ± 0.366
1.895GlnThr: 1.895 ± 0.333
2.579GlnVal: 2.579 ± 0.351
0.684GlnTrp: 0.684 ± 0.171
0.737GlnTyr: 0.737 ± 0.236
0.0GlnXaa: 0.0 ± 0.0
Arg
7.368ArgAla: 7.368 ± 0.7
1.263ArgCys: 1.263 ± 0.302
4.736ArgAsp: 4.736 ± 0.512
4.894ArgGlu: 4.894 ± 0.579
2.052ArgPhe: 2.052 ± 0.351
4.421ArgGly: 4.421 ± 0.46
1.263ArgHis: 1.263 ± 0.247
3.631ArgIle: 3.631 ± 0.483
2.631ArgLys: 2.631 ± 0.42
4.894ArgLeu: 4.894 ± 0.577
2.368ArgMet: 2.368 ± 0.37
2.263ArgAsn: 2.263 ± 0.377
3.684ArgPro: 3.684 ± 0.461
1.474ArgGln: 1.474 ± 0.326
5.631ArgArg: 5.631 ± 0.627
3.421ArgSer: 3.421 ± 0.399
3.631ArgThr: 3.631 ± 0.519
5.263ArgVal: 5.263 ± 0.624
1.842ArgTrp: 1.842 ± 0.319
1.631ArgTyr: 1.631 ± 0.301
0.0ArgXaa: 0.0 ± 0.0
Ser
5.473SerAla: 5.473 ± 0.713
0.526SerCys: 0.526 ± 0.192
4.947SerAsp: 4.947 ± 0.596
2.947SerGlu: 2.947 ± 0.396
1.947SerPhe: 1.947 ± 0.349
6.315SerGly: 6.315 ± 0.799
1.158SerHis: 1.158 ± 0.232
2.947SerIle: 2.947 ± 0.42
2.158SerLys: 2.158 ± 0.362
3.421SerLeu: 3.421 ± 0.43
1.421SerMet: 1.421 ± 0.24
2.473SerAsn: 2.473 ± 0.365
4.052SerPro: 4.052 ± 0.399
2.052SerGln: 2.052 ± 0.273
3.631SerArg: 3.631 ± 0.386
4.315SerSer: 4.315 ± 0.611
3.421SerThr: 3.421 ± 0.487
4.789SerVal: 4.789 ± 0.534
1.316SerTrp: 1.316 ± 0.31
1.526SerTyr: 1.526 ± 0.218
0.0SerXaa: 0.0 ± 0.0
Thr
6.315ThrAla: 6.315 ± 0.63
0.684ThrCys: 0.684 ± 0.222
4.105ThrAsp: 4.105 ± 0.575
3.894ThrGlu: 3.894 ± 0.399
1.789ThrPhe: 1.789 ± 0.362
6.42ThrGly: 6.42 ± 0.64
1.631ThrHis: 1.631 ± 0.3
3.526ThrIle: 3.526 ± 0.516
2.105ThrLys: 2.105 ± 0.344
4.21ThrLeu: 4.21 ± 0.552
1.368ThrMet: 1.368 ± 0.275
2.0ThrAsn: 2.0 ± 0.283
4.947ThrPro: 4.947 ± 0.61
1.737ThrGln: 1.737 ± 0.308
3.894ThrArg: 3.894 ± 0.446
4.0ThrSer: 4.0 ± 0.484
4.315ThrThr: 4.315 ± 0.586
5.526ThrVal: 5.526 ± 0.618
1.158ThrTrp: 1.158 ± 0.235
1.842ThrTyr: 1.842 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
7.473ValAla: 7.473 ± 0.554
1.316ValCys: 1.316 ± 0.24
5.105ValAsp: 5.105 ± 0.524
4.842ValGlu: 4.842 ± 0.445
2.052ValPhe: 2.052 ± 0.364
5.157ValGly: 5.157 ± 0.615
1.526ValHis: 1.526 ± 0.251
3.0ValIle: 3.0 ± 0.368
2.737ValLys: 2.737 ± 0.442
5.42ValLeu: 5.42 ± 0.563
1.368ValMet: 1.368 ± 0.228
1.947ValAsn: 1.947 ± 0.35
4.157ValPro: 4.157 ± 0.387
2.737ValGln: 2.737 ± 0.337
4.473ValArg: 4.473 ± 0.512
4.894ValSer: 4.894 ± 0.475
4.842ValThr: 4.842 ± 0.493
5.947ValVal: 5.947 ± 0.709
1.631ValTrp: 1.631 ± 0.318
1.158ValTyr: 1.158 ± 0.238
0.0ValXaa: 0.0 ± 0.0
Trp
2.368TrpAla: 2.368 ± 0.374
0.421TrpCys: 0.421 ± 0.165
1.421TrpAsp: 1.421 ± 0.304
1.158TrpGlu: 1.158 ± 0.324
0.737TrpPhe: 0.737 ± 0.226
1.0TrpGly: 1.0 ± 0.254
0.421TrpHis: 0.421 ± 0.152
1.053TrpIle: 1.053 ± 0.205
0.842TrpLys: 0.842 ± 0.174
1.474TrpLeu: 1.474 ± 0.306
1.0TrpMet: 1.0 ± 0.234
0.632TrpAsn: 0.632 ± 0.194
1.105TrpPro: 1.105 ± 0.216
0.947TrpGln: 0.947 ± 0.233
2.158TrpArg: 2.158 ± 0.399
1.316TrpSer: 1.316 ± 0.316
1.526TrpThr: 1.526 ± 0.264
1.789TrpVal: 1.789 ± 0.405
1.263TrpTrp: 1.263 ± 0.252
0.632TrpTyr: 0.632 ± 0.23
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.947TyrAla: 1.947 ± 0.342
0.158TyrCys: 0.158 ± 0.089
1.526TyrAsp: 1.526 ± 0.37
2.473TyrGlu: 2.473 ± 0.34
0.842TyrPhe: 0.842 ± 0.229
2.263TyrGly: 2.263 ± 0.31
0.368TyrHis: 0.368 ± 0.115
1.21TyrIle: 1.21 ± 0.224
0.632TyrLys: 0.632 ± 0.184
1.526TyrLeu: 1.526 ± 0.254
0.211TyrMet: 0.211 ± 0.094
0.684TyrAsn: 0.684 ± 0.166
1.368TyrPro: 1.368 ± 0.28
0.684TyrGln: 0.684 ± 0.213
2.052TyrArg: 2.052 ± 0.38
0.895TyrSer: 0.895 ± 0.221
1.316TyrThr: 1.316 ± 0.292
2.21TyrVal: 2.21 ± 0.277
0.684TyrTrp: 0.684 ± 0.185
0.526TyrTyr: 0.526 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 108 proteins (19003 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski