Firmicutes bacterium CAG:449

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6S9J3|R6S9J3_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:449 OX=1263023 GN=BN661_00123 PE=4 SV=1
MM1 pKa = 7.78ISPSNNISYY10 pKa = 11.0LEE12 pKa = 4.33TNFSQTSFSLDD23 pKa = 3.18EE24 pKa = 4.24VGTYY28 pKa = 10.15QFDD31 pKa = 3.62FLINNEE37 pKa = 3.45IKK39 pKa = 9.45TYY41 pKa = 10.12KK42 pKa = 10.1IYY44 pKa = 11.3VEE46 pKa = 4.27FDD48 pKa = 3.28SEE50 pKa = 4.08EE51 pKa = 3.92GKK53 pKa = 10.44IFDD56 pKa = 4.09NQTSIDD62 pKa = 4.72LINNSNNLKK71 pKa = 10.33FDD73 pKa = 4.91GILDD77 pKa = 3.57NLFIVILLAAILFSIDD93 pKa = 3.14WMWYY97 pKa = 9.56SYY99 pKa = 7.3EE100 pKa = 3.92QRR102 pKa = 3.61

Molecular weight: 11.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6R1D2|R6R1D2_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:449 OX=1263023 GN=BN661_01001 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 10.32RR10 pKa = 11.84KK11 pKa = 7.77HH12 pKa = 4.94TSVNGFRR19 pKa = 11.84ARR21 pKa = 11.84MATPNGRR28 pKa = 11.84KK29 pKa = 8.92VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.43GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1770

0

1770

515719

30

1811

291.4

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.643 ± 0.063

1.374 ± 0.023

5.762 ± 0.049

6.765 ± 0.052

5.068 ± 0.059

5.392 ± 0.059

1.528 ± 0.022

9.505 ± 0.08

9.038 ± 0.059

9.423 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.027

7.479 ± 0.07

2.557 ± 0.027

2.641 ± 0.027

2.734 ± 0.038

6.945 ± 0.057

5.179 ± 0.056

5.791 ± 0.053

0.664 ± 0.021

5.282 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski