Acanthamoeba polyphaga mimivirus (APMV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; Mimivirus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 909 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q5UPV1|YL271_MIMIV Putative ankyrin repeat protein L271 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L271 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 4.5FNKK5 pKa = 10.56LYY7 pKa = 11.17VLAQNNLFTDD17 pKa = 3.54ITIILKK23 pKa = 10.6DD24 pKa = 3.76EE25 pKa = 4.59SNEE28 pKa = 3.65ITLNLHH34 pKa = 6.0KK35 pKa = 10.82NIIYY39 pKa = 9.68SSCIFFEE46 pKa = 4.41KK47 pKa = 10.67LLTSFKK53 pKa = 10.39EE54 pKa = 4.14KK55 pKa = 10.26EE56 pKa = 3.94SSKK59 pKa = 10.74IILNVPNAIIVNDD72 pKa = 4.98IIWDD76 pKa = 4.44FYY78 pKa = 8.44GQKK81 pKa = 9.64IKK83 pKa = 10.65SHH85 pKa = 6.8NYY87 pKa = 8.99PEE89 pKa = 3.68WKK91 pKa = 10.51YY92 pKa = 10.62FIEE95 pKa = 4.47SYY97 pKa = 10.97KK98 pKa = 10.89CFDD101 pKa = 3.8YY102 pKa = 11.31FGMNTDD108 pKa = 3.05KK109 pKa = 11.22KK110 pKa = 10.54KK111 pKa = 10.41LYY113 pKa = 10.29KK114 pKa = 10.64LIVEE118 pKa = 4.31PDD120 pKa = 3.53GFDD123 pKa = 3.31EE124 pKa = 6.12LMDD127 pKa = 4.92FIDD130 pKa = 5.95LIGYY134 pKa = 9.07DD135 pKa = 3.65EE136 pKa = 4.4NAIEE140 pKa = 4.97VIFNNLPDD148 pKa = 5.5DD149 pKa = 4.32YY150 pKa = 11.28DD151 pKa = 3.66LSKK154 pKa = 10.82FSHH157 pKa = 6.21EE158 pKa = 4.99LLTEE162 pKa = 3.95MLSMYY167 pKa = 10.24KK168 pKa = 10.27KK169 pKa = 10.48NLVQIITRR177 pKa = 11.84NEE179 pKa = 3.86IIVLNDD185 pKa = 3.35FFDD188 pKa = 3.62GTIRR192 pKa = 11.84STEE195 pKa = 3.74HH196 pKa = 6.98CINSPEE202 pKa = 4.23VILFCASNASKK213 pKa = 10.48IVTFDD218 pKa = 3.13GHH220 pKa = 6.68NIRR223 pKa = 11.84ILEE226 pKa = 4.29SNNSILLSDD235 pKa = 4.85DD236 pKa = 3.54STVSDD241 pKa = 4.33AGTILHH247 pKa = 7.03ISHH250 pKa = 6.34SQNKK254 pKa = 8.09NVLVILYY261 pKa = 7.6EE262 pKa = 3.85YY263 pKa = 10.39RR264 pKa = 11.84IDD266 pKa = 3.55VWDD269 pKa = 3.6VLINKK274 pKa = 8.84LIASFRR280 pKa = 11.84VPFIKK285 pKa = 10.33KK286 pKa = 10.17LSCSYY291 pKa = 10.94DD292 pKa = 3.18GSQLVYY298 pKa = 10.87VDD300 pKa = 4.45NNNNMIVKK308 pKa = 10.18NILSEE313 pKa = 4.33EE314 pKa = 4.21IISEE318 pKa = 4.05INLQNLPEE326 pKa = 4.32ISNNFCIPEE335 pKa = 5.12LILEE339 pKa = 4.56TNTQDD344 pKa = 4.97LSEE347 pKa = 4.74IPNNLYY353 pKa = 9.03TLEE356 pKa = 4.67LKK358 pKa = 10.48SEE360 pKa = 4.45PDD362 pKa = 3.22QSIDD366 pKa = 3.86FIKK369 pKa = 11.13SNFQHH374 pKa = 6.64NLDD377 pKa = 4.93NLDD380 pKa = 4.67DD381 pKa = 5.41LNNLDD386 pKa = 5.45NSDD389 pKa = 5.28DD390 pKa = 4.14LDD392 pKa = 4.05NSNDD396 pKa = 3.74LNDD399 pKa = 5.08SNDD402 pKa = 4.08LDD404 pKa = 5.83DD405 pKa = 6.12SDD407 pKa = 6.19DD408 pKa = 4.53SNDD411 pKa = 3.84YY412 pKa = 11.72NNLNYY417 pKa = 10.52DD418 pKa = 4.1LNNLEE423 pKa = 5.25IICSSNNEE431 pKa = 3.46IFEE434 pKa = 4.42NVSTHH439 pKa = 5.17YY440 pKa = 10.96CIDD443 pKa = 3.7VNDD446 pKa = 4.81CNHH449 pKa = 6.78IDD451 pKa = 4.51NINSLNLQSPIQDD464 pKa = 3.86LSDD467 pKa = 3.45SLSPIDD473 pKa = 5.52YY474 pKa = 9.86IDD476 pKa = 3.83SSNLQSPVQDD486 pKa = 4.22LSDD489 pKa = 3.99SQSSTDD495 pKa = 3.66WIVSPNTQSPVYY507 pKa = 9.95YY508 pKa = 10.41LSKK511 pKa = 10.91LDD513 pKa = 3.69GSINGEE519 pKa = 4.16KK520 pKa = 10.6LSDD523 pKa = 3.55ALSIEE528 pKa = 4.19NDD530 pKa = 4.01SISDD534 pKa = 3.97NSDD537 pKa = 3.18NLNNSDD543 pKa = 5.2NSDD546 pKa = 4.39DD547 pKa = 5.53LDD549 pKa = 4.73NPDD552 pKa = 4.9NSDD555 pKa = 3.56NLDD558 pKa = 3.54NSDD561 pKa = 3.89YY562 pKa = 11.16LLEE565 pKa = 4.12YY566 pKa = 10.53LVNTDD571 pKa = 2.65KK572 pKa = 11.2TNYY575 pKa = 8.72MFNLTYY581 pKa = 10.45SIHH584 pKa = 5.86TDD586 pKa = 2.83KK587 pKa = 11.13TNKK590 pKa = 9.68RR591 pKa = 11.84FNLEE595 pKa = 3.65NPYY598 pKa = 10.74EE599 pKa = 4.03QFNFINKK606 pKa = 9.17FCLLYY611 pKa = 10.57INNKK615 pKa = 9.02VIYY618 pKa = 9.62FIDD621 pKa = 4.86IIDD624 pKa = 3.56KK625 pKa = 10.76KK626 pKa = 10.31IIHH629 pKa = 6.74KK630 pKa = 7.16EE631 pKa = 3.78TCRR634 pKa = 11.84RR635 pKa = 11.84NIVNMRR641 pKa = 11.84CSPTGDD647 pKa = 3.5SIAIIDD653 pKa = 3.87SEE655 pKa = 5.07YY656 pKa = 10.44IVYY659 pKa = 9.93IYY661 pKa = 11.16KK662 pKa = 10.68LVTDD666 pKa = 4.21SNGFLCYY673 pKa = 9.98PIVDD677 pKa = 3.62YY678 pKa = 11.22CILVRR683 pKa = 11.84DD684 pKa = 4.63LFLPTKK690 pKa = 10.16IEE692 pKa = 3.94YY693 pKa = 10.24SSNGRR698 pKa = 11.84YY699 pKa = 9.17LVFDD703 pKa = 5.04DD704 pKa = 5.99DD705 pKa = 4.38GLSINLYY712 pKa = 10.41DD713 pKa = 4.26IEE715 pKa = 4.12QLEE718 pKa = 4.32MTNEE722 pKa = 3.86FEE724 pKa = 5.04LFCDD728 pKa = 3.39SKK730 pKa = 11.13IIVDD734 pKa = 3.31ITFSEE739 pKa = 4.91NYY741 pKa = 9.41CDD743 pKa = 4.25EE744 pKa = 5.22LINRR748 pKa = 11.84LNNALKK754 pKa = 10.31KK755 pKa = 10.15IEE757 pKa = 4.17QKK759 pKa = 10.98YY760 pKa = 9.64PNN762 pKa = 3.88

Molecular weight: 88.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q5UQ00|FPG_MIMIV Probable formamidopyrimidine-DNA glycosylase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L315 PE=1 SV=3
MM1 pKa = 7.52TSLNTNVEE9 pKa = 4.05HH10 pKa = 7.12RR11 pKa = 11.84RR12 pKa = 11.84TRR14 pKa = 11.84TTISQIIHH22 pKa = 7.38DD23 pKa = 4.46MTPEE27 pKa = 3.6NKK29 pKa = 9.9FYY31 pKa = 10.09ITVADD36 pKa = 4.08GGSKK40 pKa = 10.27RR41 pKa = 11.84FRR43 pKa = 11.84NKK45 pKa = 10.21PNDD48 pKa = 3.44QSRR51 pKa = 11.84HH52 pKa = 4.74SGQYY56 pKa = 8.27QPRR59 pKa = 11.84NLSGKK64 pKa = 7.56TNISTQSQFTPRR76 pKa = 11.84LYY78 pKa = 10.72GKK80 pKa = 9.76QYY82 pKa = 11.23NYY84 pKa = 10.35NQPNRR89 pKa = 11.84LQTRR93 pKa = 11.84SVRR96 pKa = 11.84PNYY99 pKa = 10.2YY100 pKa = 9.75SGYY103 pKa = 9.33RR104 pKa = 11.84YY105 pKa = 10.58NNRR108 pKa = 11.84TGNQEE113 pKa = 4.69FYY115 pKa = 11.02PMQPFNNQSFNNQSRR130 pKa = 11.84THH132 pKa = 5.28QSKK135 pKa = 8.71TYY137 pKa = 9.02QHH139 pKa = 5.97NQQKK143 pKa = 10.51RR144 pKa = 11.84SFNGPRR150 pKa = 11.84NNGPQNNVPRR160 pKa = 11.84FFQRR164 pKa = 11.84EE165 pKa = 4.0EE166 pKa = 4.23TNN168 pKa = 3.15

Molecular weight: 20.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

909

0

909

341649

81

2959

375.9

43.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.072 ± 0.062

1.801 ± 0.048

6.795 ± 0.071

5.493 ± 0.072

4.617 ± 0.059

4.642 ± 0.216

2.054 ± 0.036

9.86 ± 0.101

8.981 ± 0.128

8.16 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.033

8.883 ± 0.101

3.225 ± 0.059

3.137 ± 0.056

3.292 ± 0.056

7.382 ± 0.072

5.253 ± 0.056

5.054 ± 0.062

0.738 ± 0.022

5.435 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski