Mycolicibacterium fortuitum (Mycobacterium fortuitum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9Y3C8|A0A0N9Y3C8_MYCFO Uncharacterized protein OS=Mycolicibacterium fortuitum OX=1766 GN=A5751_32160 PE=4 SV=1
MM1 pKa = 7.57SGPNPPEE8 pKa = 4.52PGASGSDD15 pKa = 4.04LPDD18 pKa = 3.13QPGKK22 pKa = 10.17HH23 pKa = 5.96SAPEE27 pKa = 3.92EE28 pKa = 3.97PTQVSGTDD36 pKa = 3.16ADD38 pKa = 4.59GEE40 pKa = 4.86TEE42 pKa = 4.88FYY44 pKa = 10.29SQAYY48 pKa = 8.25SAPEE52 pKa = 3.93SEE54 pKa = 4.76QFTSGPYY61 pKa = 9.8VPADD65 pKa = 3.42VALYY69 pKa = 10.36DD70 pKa = 3.9YY71 pKa = 11.36DD72 pKa = 6.19DD73 pKa = 4.74YY74 pKa = 12.13DD75 pKa = 3.91PATEE79 pKa = 4.65LVDD82 pKa = 3.66TAPPPRR88 pKa = 11.84WPWVVGITAIIAAVALVASVAVLVTRR114 pKa = 11.84DD115 pKa = 3.35EE116 pKa = 4.41DD117 pKa = 4.09TTNLATPHH125 pKa = 7.03PSTTTSLPPVQDD137 pKa = 4.56EE138 pKa = 4.58ITTTTPPPPPPTSEE152 pKa = 4.35EE153 pKa = 4.28LPPPPPPEE161 pKa = 4.0TVTVTEE167 pKa = 4.82PPPPAPAPTSEE178 pKa = 4.81APPPAPSTTSAPPTTTTTHH197 pKa = 6.57AGPRR201 pKa = 11.84QVTYY205 pKa = 11.02SVTGTKK211 pKa = 10.45APGDD215 pKa = 4.39IITITYY221 pKa = 10.34VDD223 pKa = 3.55GNGNRR228 pKa = 11.84RR229 pKa = 11.84TLRR232 pKa = 11.84NVYY235 pKa = 9.98IPWTFTMTPISNSDD249 pKa = 3.4VGSVEE254 pKa = 3.81ASSLFLVSRR263 pKa = 11.84LNCSITASDD272 pKa = 3.87GTVLSSNANNSAQTACC288 pKa = 4.32

Molecular weight: 29.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9YKT1|A0A0N9YKT1_MYCFO ATP-dependent DNA helicase OS=Mycolicibacterium fortuitum OX=1766 GN=pcrA PE=3 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.11GKK5 pKa = 8.48RR6 pKa = 11.84TYY8 pKa = 10.27QPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVANRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.67GRR42 pKa = 11.84RR43 pKa = 11.84ALTAA47 pKa = 3.88

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6012

0

6012

1901592

37

8102

316.3

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.951 ± 0.039

0.784 ± 0.009

6.275 ± 0.024

5.339 ± 0.026

3.076 ± 0.016

8.812 ± 0.03

2.192 ± 0.014

4.31 ± 0.024

2.302 ± 0.022

9.814 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.106 ± 0.015

2.284 ± 0.017

5.804 ± 0.028

3.084 ± 0.019

6.976 ± 0.029

5.466 ± 0.016

6.076 ± 0.023

8.645 ± 0.03

1.525 ± 0.015

2.177 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski