Arthrobacter sp. MN05-02

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9WF81|A0A3Q9WF81_9MICC Arginine biosynthesis bifunctional protein ArgJ OS=Arthrobacter sp. MN05-02 OX=1571833 GN=argJ PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.44SKK4 pKa = 10.49ISPAVLRR11 pKa = 11.84GSALALAAALALTACGGGGNDD32 pKa = 4.51AGGAAPAEE40 pKa = 4.35VSADD44 pKa = 3.72DD45 pKa = 3.78VQAALDD51 pKa = 3.66KK52 pKa = 11.66GGDD55 pKa = 3.54LTVWAWEE62 pKa = 3.88PTLEE66 pKa = 4.61KK67 pKa = 10.7VVEE70 pKa = 4.3DD71 pKa = 5.72FEE73 pKa = 6.28AEE75 pKa = 4.14HH76 pKa = 6.8PNVNIDD82 pKa = 3.93LVNAGTGNDD91 pKa = 3.59QYY93 pKa = 11.47TALQNAIAAGSGVPDD108 pKa = 3.67VAQIEE113 pKa = 5.14YY114 pKa = 10.6YY115 pKa = 10.86ALPQFALAEE124 pKa = 4.36SVQDD128 pKa = 3.55LSGYY132 pKa = 9.91GAGDD136 pKa = 3.53LQDD139 pKa = 3.85TFSPGPWSSVQANEE153 pKa = 4.67GIYY156 pKa = 10.01GLPMDD161 pKa = 5.41SGPMALFYY169 pKa = 10.15NTEE172 pKa = 3.86VFEE175 pKa = 4.68KK176 pKa = 10.88YY177 pKa = 10.2GVEE180 pKa = 4.98VPTTWEE186 pKa = 4.24EE187 pKa = 3.94YY188 pKa = 10.55VEE190 pKa = 4.2AARR193 pKa = 11.84TLHH196 pKa = 6.37EE197 pKa = 5.37ADD199 pKa = 3.56PDD201 pKa = 3.99VYY203 pKa = 10.59IANDD207 pKa = 3.3TGDD210 pKa = 3.73AGFATSLIWQAGGQPYY226 pKa = 8.28TVDD229 pKa = 3.36GTDD232 pKa = 3.13VKK234 pKa = 11.43VDD236 pKa = 4.17FSDD239 pKa = 3.52EE240 pKa = 4.19GSTKK244 pKa = 10.05FAEE247 pKa = 4.5TWQQLLDD254 pKa = 4.14EE255 pKa = 5.53DD256 pKa = 4.49LLAPISSWSDD266 pKa = 2.5EE267 pKa = 4.18WYY269 pKa = 10.44QGLGTGTIATLATGAWMPGNFTSGVPAAAGKK300 pKa = 8.54WRR302 pKa = 11.84AAPLPQWEE310 pKa = 4.5EE311 pKa = 4.02GGTASAEE318 pKa = 4.15NGGSSLTIPAASEE331 pKa = 3.84QGALAYY337 pKa = 10.48AFLDD341 pKa = 3.83YY342 pKa = 11.48ANVGDD347 pKa = 4.54GVQTRR352 pKa = 11.84IDD354 pKa = 3.57GGAFPATSAALEE366 pKa = 4.0SDD368 pKa = 3.46EE369 pKa = 4.29FLNSEE374 pKa = 4.22FEE376 pKa = 4.32YY377 pKa = 11.03FGGQKK382 pKa = 10.45ANEE385 pKa = 4.08IFAEE389 pKa = 4.39SAQNVPEE396 pKa = 4.39GWSYY400 pKa = 11.82LPFQVYY406 pKa = 10.66ANSIFNDD413 pKa = 3.97TVGQAYY419 pKa = 10.45VSDD422 pKa = 4.07TPLKK426 pKa = 10.82DD427 pKa = 4.23GLKK430 pKa = 9.18SWQDD434 pKa = 3.03ASIKK438 pKa = 10.8YY439 pKa = 8.32GTEE442 pKa = 3.36QGFTVSEE449 pKa = 4.18

Molecular weight: 47.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0Y8M2|A0A3T0Y8M2_9MICC Coenzyme A pyrophosphatase OS=Arthrobacter sp. MN05-02 OX=1571833 GN=MN0502_27990 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.31VRR4 pKa = 11.84NSLRR8 pKa = 11.84ALKK11 pKa = 9.65KK12 pKa = 10.17IPGAQIVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GKK24 pKa = 8.33TLVINKK30 pKa = 9.19KK31 pKa = 8.38NPRR34 pKa = 11.84MKK36 pKa = 10.47ARR38 pKa = 11.84QGG40 pKa = 3.24

Molecular weight: 4.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3539

0

3539

1059819

39

1826

299.5

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.957 ± 0.052

0.607 ± 0.01

6.101 ± 0.036

5.621 ± 0.039

3.076 ± 0.023

9.265 ± 0.038

2.093 ± 0.021

4.121 ± 0.033

2.028 ± 0.026

10.261 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.826 ± 0.016

2.08 ± 0.023

5.645 ± 0.034

3.017 ± 0.022

7.358 ± 0.047

5.889 ± 0.027

6.091 ± 0.037

8.574 ± 0.037

1.371 ± 0.016

2.019 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski