Arsenophonus nasoniae (son-killer infecting Nasonia vitripennis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Morganellaceae; Arsenophonus

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4741 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2U259|D2U259_9GAMM Phosphatidate cytidylyltransferase OS=Arsenophonus nasoniae OX=638 GN=cdsA PE=3 SV=1
MM1 pKa = 6.68TTRR4 pKa = 11.84CKK6 pKa = 10.1MKK8 pKa = 9.94IGLFYY13 pKa = 10.93GSSTCYY19 pKa = 10.32TEE21 pKa = 4.37MVAEE25 pKa = 5.03KK26 pKa = 10.05IQYY29 pKa = 9.88NLGEE33 pKa = 4.4EE34 pKa = 4.65LVDD37 pKa = 4.6LYY39 pKa = 11.01NIKK42 pKa = 10.51DD43 pKa = 3.46ISPQLMEE50 pKa = 4.84NYY52 pKa = 9.89QILILGIPTWDD63 pKa = 3.34FGEE66 pKa = 4.62LQEE69 pKa = 4.74DD70 pKa = 4.03WQTIWDD76 pKa = 4.21EE77 pKa = 4.27LCSLNLAGKK86 pKa = 9.53IVSLYY91 pKa = 11.17GLGDD95 pKa = 3.64QIDD98 pKa = 3.89YY99 pKa = 11.45GDD101 pKa = 3.94WFLDD105 pKa = 3.55ALGMLYY111 pKa = 9.82QQLSRR116 pKa = 11.84MQVKK120 pKa = 9.1FVGFWPTTGYY130 pKa = 10.74NFSSLKK136 pKa = 10.6PLTTDD141 pKa = 3.34GNQFVGLALDD151 pKa = 3.57EE152 pKa = 4.55TNQFEE157 pKa = 4.5QTDD160 pKa = 3.61EE161 pKa = 6.02RR162 pKa = 11.84IEE164 pKa = 3.97QWCMQILNEE173 pKa = 4.84IEE175 pKa = 4.09ALLL178 pKa = 4.07

Molecular weight: 20.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2TY75|D2TY75_9GAMM Ribosomal RNA small subunit methyltransferase G OS=Arsenophonus nasoniae OX=638 GN=gidB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSSKK47 pKa = 11.16

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4741

0

4741

1228501

29

2821

259.1

29.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.723 ± 0.041

1.147 ± 0.015

5.012 ± 0.03

5.692 ± 0.038

4.141 ± 0.028

6.102 ± 0.037

2.274 ± 0.023

7.741 ± 0.034

6.54 ± 0.043

10.275 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.019

5.289 ± 0.044

3.852 ± 0.022

4.811 ± 0.036

4.84 ± 0.032

6.23 ± 0.028

5.445 ± 0.039

5.769 ± 0.029

1.257 ± 0.017

3.414 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski