Amino acid dipepetide frequency for Cyanophage P-TIM40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.862AlaAla: 5.862 ± 0.412
0.537AlaCys: 0.537 ± 0.111
4.364AlaAsp: 4.364 ± 0.318
3.875AlaGlu: 3.875 ± 0.318
2.573AlaPhe: 2.573 ± 0.306
5.911AlaGly: 5.911 ± 0.434
1.107AlaHis: 1.107 ± 0.155
4.559AlaIle: 4.559 ± 0.459
3.973AlaLys: 3.973 ± 0.467
4.559AlaLeu: 4.559 ± 0.259
1.237AlaMet: 1.237 ± 0.211
3.859AlaAsn: 3.859 ± 0.318
2.345AlaPro: 2.345 ± 0.188
2.524AlaGln: 2.524 ± 0.201
2.67AlaArg: 2.67 ± 0.213
4.527AlaSer: 4.527 ± 0.436
5.715AlaThr: 5.715 ± 0.67
4.315AlaVal: 4.315 ± 0.291
0.586AlaTrp: 0.586 ± 0.08
2.703AlaTyr: 2.703 ± 0.219
0.0AlaXaa: 0.0 ± 0.0
Cys
0.488CysAla: 0.488 ± 0.089
0.098CysCys: 0.098 ± 0.042
0.602CysAsp: 0.602 ± 0.125
0.765CysGlu: 0.765 ± 0.134
0.44CysPhe: 0.44 ± 0.111
0.57CysGly: 0.57 ± 0.122
0.195CysHis: 0.195 ± 0.063
0.733CysIle: 0.733 ± 0.114
0.505CysLys: 0.505 ± 0.092
0.765CysLeu: 0.765 ± 0.141
0.228CysMet: 0.228 ± 0.072
0.488CysAsn: 0.488 ± 0.091
0.375CysPro: 0.375 ± 0.089
0.375CysGln: 0.375 ± 0.098
0.309CysArg: 0.309 ± 0.079
0.7CysSer: 0.7 ± 0.123
0.586CysThr: 0.586 ± 0.089
0.765CysVal: 0.765 ± 0.137
0.081CysTrp: 0.081 ± 0.036
0.651CysTyr: 0.651 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
4.869AspAla: 4.869 ± 0.38
0.782AspCys: 0.782 ± 0.147
4.641AspAsp: 4.641 ± 0.346
3.664AspGlu: 3.664 ± 0.333
3.191AspPhe: 3.191 ± 0.243
5.292AspGly: 5.292 ± 0.472
1.189AspHis: 1.189 ± 0.167
4.364AspIle: 4.364 ± 0.427
3.68AspLys: 3.68 ± 0.449
5.52AspLeu: 5.52 ± 0.357
1.759AspMet: 1.759 ± 0.235
3.81AspAsn: 3.81 ± 0.349
3.778AspPro: 3.778 ± 0.274
2.394AspGln: 2.394 ± 0.248
2.931AspArg: 2.931 ± 0.252
3.712AspSer: 3.712 ± 0.26
4.315AspThr: 4.315 ± 0.341
4.51AspVal: 4.51 ± 0.33
1.042AspTrp: 1.042 ± 0.152
3.517AspTyr: 3.517 ± 0.263
0.0AspXaa: 0.0 ± 0.0
Glu
3.973GluAla: 3.973 ± 0.267
0.733GluCys: 0.733 ± 0.123
3.761GluAsp: 3.761 ± 0.308
4.103GluGlu: 4.103 ± 0.437
3.175GluPhe: 3.175 ± 0.213
4.51GluGly: 4.51 ± 0.228
0.928GluHis: 0.928 ± 0.151
4.087GluIle: 4.087 ± 0.257
3.94GluLys: 3.94 ± 0.517
3.94GluLeu: 3.94 ± 0.406
1.384GluMet: 1.384 ± 0.19
2.752GluAsn: 2.752 ± 0.218
1.384GluPro: 1.384 ± 0.141
2.035GluGln: 2.035 ± 0.234
2.361GluArg: 2.361 ± 0.168
3.533GluSer: 3.533 ± 0.274
3.566GluThr: 3.566 ± 0.202
4.331GluVal: 4.331 ± 0.23
0.993GluTrp: 0.993 ± 0.131
2.996GluTyr: 2.996 ± 0.209
0.0GluXaa: 0.0 ± 0.0
Phe
2.719PheAla: 2.719 ± 0.271
0.505PheCys: 0.505 ± 0.115
3.664PheAsp: 3.664 ± 0.308
2.328PheGlu: 2.328 ± 0.169
1.856PhePhe: 1.856 ± 0.157
3.012PheGly: 3.012 ± 0.227
0.7PheHis: 0.7 ± 0.158
2.622PheIle: 2.622 ± 0.218
2.508PheLys: 2.508 ± 0.283
2.752PheLeu: 2.752 ± 0.329
0.847PheMet: 0.847 ± 0.151
2.915PheAsn: 2.915 ± 0.25
1.84PhePro: 1.84 ± 0.203
1.693PheGln: 1.693 ± 0.152
1.693PheArg: 1.693 ± 0.158
3.159PheSer: 3.159 ± 0.243
3.436PheThr: 3.436 ± 0.26
2.247PheVal: 2.247 ± 0.166
0.537PheTrp: 0.537 ± 0.122
1.873PheTyr: 1.873 ± 0.166
0.0PheXaa: 0.0 ± 0.0
Gly
5.504GlyAla: 5.504 ± 0.516
0.619GlyCys: 0.619 ± 0.115
5.39GlyAsp: 5.39 ± 0.417
4.201GlyGlu: 4.201 ± 0.26
3.159GlyPhe: 3.159 ± 0.246
8.418GlyGly: 8.418 ± 0.923
1.27GlyHis: 1.27 ± 0.162
5.064GlyIle: 5.064 ± 0.333
4.25GlyLys: 4.25 ± 0.379
4.559GlyLeu: 4.559 ± 0.27
1.677GlyMet: 1.677 ± 0.275
5.259GlyAsn: 5.259 ± 0.697
1.759GlyPro: 1.759 ± 0.185
2.801GlyGln: 2.801 ± 0.198
3.289GlyArg: 3.289 ± 0.197
6.513GlySer: 6.513 ± 0.429
5.715GlyThr: 5.715 ± 0.641
4.738GlyVal: 4.738 ± 0.249
1.172GlyTrp: 1.172 ± 0.163
3.159GlyTyr: 3.159 ± 0.306
0.0GlyXaa: 0.0 ± 0.0
His
0.912HisAla: 0.912 ± 0.118
0.261HisCys: 0.261 ± 0.07
1.172HisAsp: 1.172 ± 0.154
1.254HisGlu: 1.254 ± 0.218
0.863HisPhe: 0.863 ± 0.14
1.27HisGly: 1.27 ± 0.189
0.488HisHis: 0.488 ± 0.105
1.172HisIle: 1.172 ± 0.122
1.319HisLys: 1.319 ± 0.237
1.205HisLeu: 1.205 ± 0.224
0.407HisMet: 0.407 ± 0.094
1.124HisAsn: 1.124 ± 0.139
1.042HisPro: 1.042 ± 0.142
0.521HisGln: 0.521 ± 0.093
0.847HisArg: 0.847 ± 0.151
1.058HisSer: 1.058 ± 0.133
0.928HisThr: 0.928 ± 0.105
0.977HisVal: 0.977 ± 0.12
0.293HisTrp: 0.293 ± 0.073
0.912HisTyr: 0.912 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
3.778IleAla: 3.778 ± 0.375
0.668IleCys: 0.668 ± 0.154
4.95IleAsp: 4.95 ± 0.323
4.347IleGlu: 4.347 ± 0.278
2.638IlePhe: 2.638 ± 0.207
4.282IleGly: 4.282 ± 0.326
1.091IleHis: 1.091 ± 0.158
3.761IleIle: 3.761 ± 0.316
4.38IleLys: 4.38 ± 0.343
4.282IleLeu: 4.282 ± 0.262
1.026IleMet: 1.026 ± 0.155
3.989IleAsn: 3.989 ± 0.253
3.094IlePro: 3.094 ± 0.223
2.491IleGln: 2.491 ± 0.24
2.328IleArg: 2.328 ± 0.223
4.087IleSer: 4.087 ± 0.362
5.731IleThr: 5.731 ± 0.67
3.859IleVal: 3.859 ± 0.319
0.619IleTrp: 0.619 ± 0.107
2.394IleTyr: 2.394 ± 0.288
0.0IleXaa: 0.0 ± 0.0
Lys
3.729LysAla: 3.729 ± 0.344
0.505LysCys: 0.505 ± 0.094
4.038LysAsp: 4.038 ± 0.419
4.364LysGlu: 4.364 ± 0.473
2.28LysPhe: 2.28 ± 0.168
3.908LysGly: 3.908 ± 0.429
1.172LysHis: 1.172 ± 0.186
4.413LysIle: 4.413 ± 0.287
5.08LysLys: 5.08 ± 0.828
4.429LysLeu: 4.429 ± 0.41
1.612LysMet: 1.612 ± 0.247
3.191LysAsn: 3.191 ± 0.265
2.296LysPro: 2.296 ± 0.302
2.166LysGln: 2.166 ± 0.274
2.703LysArg: 2.703 ± 0.396
3.664LysSer: 3.664 ± 0.412
3.859LysThr: 3.859 ± 0.368
4.136LysVal: 4.136 ± 0.36
1.01LysTrp: 1.01 ± 0.129
3.029LysTyr: 3.029 ± 0.289
0.0LysXaa: 0.0 ± 0.0
Leu
4.543LeuAla: 4.543 ± 0.253
0.651LeuCys: 0.651 ± 0.124
5.911LeuAsp: 5.911 ± 0.377
4.299LeuGlu: 4.299 ± 0.389
2.866LeuPhe: 2.866 ± 0.219
4.331LeuGly: 4.331 ± 0.382
1.27LeuHis: 1.27 ± 0.166
3.843LeuIle: 3.843 ± 0.288
4.82LeuLys: 4.82 ± 0.373
4.364LeuLeu: 4.364 ± 0.332
1.498LeuMet: 1.498 ± 0.216
4.38LeuAsn: 4.38 ± 0.254
3.061LeuPro: 3.061 ± 0.273
2.866LeuGln: 2.866 ± 0.221
3.224LeuArg: 3.224 ± 0.269
4.592LeuSer: 4.592 ± 0.3
5.585LeuThr: 5.585 ± 0.449
4.038LeuVal: 4.038 ± 0.27
0.635LeuTrp: 0.635 ± 0.118
2.801LeuTyr: 2.801 ± 0.265
0.0LeuXaa: 0.0 ± 0.0
Met
1.759MetAla: 1.759 ± 0.221
0.293MetCys: 0.293 ± 0.095
1.303MetAsp: 1.303 ± 0.184
1.091MetGlu: 1.091 ± 0.198
0.7MetPhe: 0.7 ± 0.136
1.156MetGly: 1.156 ± 0.161
0.537MetHis: 0.537 ± 0.13
1.237MetIle: 1.237 ± 0.178
1.71MetLys: 1.71 ± 0.309
1.4MetLeu: 1.4 ± 0.194
0.521MetMet: 0.521 ± 0.099
1.221MetAsn: 1.221 ± 0.221
0.944MetPro: 0.944 ± 0.176
0.765MetGln: 0.765 ± 0.155
1.091MetArg: 1.091 ± 0.174
1.954MetSer: 1.954 ± 0.301
1.628MetThr: 1.628 ± 0.223
1.124MetVal: 1.124 ± 0.159
0.391MetTrp: 0.391 ± 0.114
0.684MetTyr: 0.684 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
3.582AsnAla: 3.582 ± 0.326
0.57AsnCys: 0.57 ± 0.12
3.452AsnAsp: 3.452 ± 0.261
3.289AsnGlu: 3.289 ± 0.235
2.573AsnPhe: 2.573 ± 0.193
4.771AsnGly: 4.771 ± 0.449
0.944AsnHis: 0.944 ± 0.106
4.071AsnIle: 4.071 ± 0.307
3.061AsnLys: 3.061 ± 0.33
4.233AsnLeu: 4.233 ± 0.386
1.237AsnMet: 1.237 ± 0.151
3.257AsnAsn: 3.257 ± 0.312
3.289AsnPro: 3.289 ± 0.286
2.345AsnGln: 2.345 ± 0.226
2.426AsnArg: 2.426 ± 0.189
4.217AsnSer: 4.217 ± 0.393
3.761AsnThr: 3.761 ± 0.372
3.761AsnVal: 3.761 ± 0.388
0.554AsnTrp: 0.554 ± 0.085
2.214AsnTyr: 2.214 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
2.426ProAla: 2.426 ± 0.236
0.261ProCys: 0.261 ± 0.094
2.98ProAsp: 2.98 ± 0.287
2.442ProGlu: 2.442 ± 0.203
1.547ProPhe: 1.547 ± 0.175
2.784ProGly: 2.784 ± 0.304
0.961ProHis: 0.961 ± 0.134
2.377ProIle: 2.377 ± 0.237
2.703ProLys: 2.703 ± 0.252
2.931ProLeu: 2.931 ± 0.161
0.782ProMet: 0.782 ± 0.183
2.426ProAsn: 2.426 ± 0.162
2.247ProPro: 2.247 ± 0.236
1.514ProGln: 1.514 ± 0.183
1.531ProArg: 1.531 ± 0.201
3.077ProSer: 3.077 ± 0.248
3.338ProThr: 3.338 ± 0.245
2.882ProVal: 2.882 ± 0.28
0.505ProTrp: 0.505 ± 0.105
2.019ProTyr: 2.019 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
2.605GlnAla: 2.605 ± 0.207
0.309GlnCys: 0.309 ± 0.083
1.921GlnAsp: 1.921 ± 0.157
2.28GlnGlu: 2.28 ± 0.24
1.612GlnPhe: 1.612 ± 0.132
2.508GlnGly: 2.508 ± 0.241
0.749GlnHis: 0.749 ± 0.128
2.214GlnIle: 2.214 ± 0.201
2.247GlnLys: 2.247 ± 0.254
2.996GlnLeu: 2.996 ± 0.222
0.928GlnMet: 0.928 ± 0.181
1.71GlnAsn: 1.71 ± 0.186
1.742GlnPro: 1.742 ± 0.163
1.4GlnGln: 1.4 ± 0.167
1.351GlnArg: 1.351 ± 0.18
2.589GlnSer: 2.589 ± 0.177
2.345GlnThr: 2.345 ± 0.238
2.524GlnVal: 2.524 ± 0.198
0.57GlnTrp: 0.57 ± 0.102
1.889GlnTyr: 1.889 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
2.247ArgAla: 2.247 ± 0.188
0.472ArgCys: 0.472 ± 0.102
2.67ArgAsp: 2.67 ± 0.253
2.312ArgGlu: 2.312 ± 0.28
2.052ArgPhe: 2.052 ± 0.156
3.175ArgGly: 3.175 ± 0.209
1.01ArgHis: 1.01 ± 0.16
2.882ArgIle: 2.882 ± 0.173
2.703ArgLys: 2.703 ± 0.3
3.191ArgLeu: 3.191 ± 0.248
1.058ArgMet: 1.058 ± 0.189
2.1ArgAsn: 2.1 ± 0.188
1.531ArgPro: 1.531 ± 0.199
1.677ArgGln: 1.677 ± 0.173
1.905ArgArg: 1.905 ± 0.245
2.394ArgSer: 2.394 ± 0.195
2.394ArgThr: 2.394 ± 0.258
3.029ArgVal: 3.029 ± 0.207
0.537ArgTrp: 0.537 ± 0.087
1.986ArgTyr: 1.986 ± 0.213
0.0ArgXaa: 0.0 ± 0.0
Ser
5.113SerAla: 5.113 ± 0.344
0.44SerCys: 0.44 ± 0.106
4.299SerAsp: 4.299 ± 0.269
3.371SerGlu: 3.371 ± 0.23
2.882SerPhe: 2.882 ± 0.162
7.246SerGly: 7.246 ± 0.506
1.091SerHis: 1.091 ± 0.161
4.185SerIle: 4.185 ± 0.266
3.452SerLys: 3.452 ± 0.363
4.689SerLeu: 4.689 ± 0.226
1.433SerMet: 1.433 ± 0.226
3.892SerAsn: 3.892 ± 0.349
2.589SerPro: 2.589 ± 0.248
2.589SerGln: 2.589 ± 0.243
2.426SerArg: 2.426 ± 0.157
5.243SerSer: 5.243 ± 0.598
4.999SerThr: 4.999 ± 0.513
4.315SerVal: 4.315 ± 0.303
0.83SerTrp: 0.83 ± 0.148
2.915SerTyr: 2.915 ± 0.214
0.0SerXaa: 0.0 ± 0.0
Thr
5.634ThrAla: 5.634 ± 0.659
0.635ThrCys: 0.635 ± 0.087
4.396ThrAsp: 4.396 ± 0.454
3.647ThrGlu: 3.647 ± 0.272
3.745ThrPhe: 3.745 ± 0.403
6.301ThrGly: 6.301 ± 0.688
1.026ThrHis: 1.026 ± 0.132
4.738ThrIle: 4.738 ± 0.446
3.761ThrLys: 3.761 ± 0.331
5.992ThrLeu: 5.992 ± 0.582
1.351ThrMet: 1.351 ± 0.186
3.908ThrAsn: 3.908 ± 0.38
3.436ThrPro: 3.436 ± 0.37
2.524ThrGln: 2.524 ± 0.246
2.377ThrArg: 2.377 ± 0.245
4.901ThrSer: 4.901 ± 0.391
5.52ThrThr: 5.52 ± 0.657
5.227ThrVal: 5.227 ± 0.457
0.733ThrTrp: 0.733 ± 0.117
2.931ThrTyr: 2.931 ± 0.247
0.0ThrXaa: 0.0 ± 0.0
Val
4.673ValAla: 4.673 ± 0.269
0.505ValCys: 0.505 ± 0.089
4.869ValAsp: 4.869 ± 0.44
3.94ValGlu: 3.94 ± 0.227
2.524ValPhe: 2.524 ± 0.195
5.373ValGly: 5.373 ± 0.396
1.075ValHis: 1.075 ± 0.153
4.071ValIle: 4.071 ± 0.326
4.038ValLys: 4.038 ± 0.3
3.843ValLeu: 3.843 ± 0.264
1.303ValMet: 1.303 ± 0.18
3.892ValAsn: 3.892 ± 0.418
2.784ValPro: 2.784 ± 0.248
1.677ValGln: 1.677 ± 0.178
2.67ValArg: 2.67 ± 0.178
4.51ValSer: 4.51 ± 0.358
5.618ValThr: 5.618 ± 0.598
4.38ValVal: 4.38 ± 0.317
0.635ValTrp: 0.635 ± 0.118
2.524ValTyr: 2.524 ± 0.224
0.0ValXaa: 0.0 ± 0.0
Trp
0.7TrpAla: 0.7 ± 0.128
0.179TrpCys: 0.179 ± 0.052
0.896TrpAsp: 0.896 ± 0.106
0.814TrpGlu: 0.814 ± 0.117
0.57TrpPhe: 0.57 ± 0.097
0.879TrpGly: 0.879 ± 0.147
0.261TrpHis: 0.261 ± 0.07
0.749TrpIle: 0.749 ± 0.111
0.847TrpLys: 0.847 ± 0.151
0.798TrpLeu: 0.798 ± 0.133
0.407TrpMet: 0.407 ± 0.113
0.863TrpAsn: 0.863 ± 0.128
0.326TrpPro: 0.326 ± 0.065
0.521TrpGln: 0.521 ± 0.091
0.57TrpArg: 0.57 ± 0.096
0.814TrpSer: 0.814 ± 0.133
0.83TrpThr: 0.83 ± 0.118
0.749TrpVal: 0.749 ± 0.105
0.212TrpTrp: 0.212 ± 0.069
0.488TrpTyr: 0.488 ± 0.116
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.687TyrAla: 2.687 ± 0.315
0.7TyrCys: 0.7 ± 0.113
3.68TyrAsp: 3.68 ± 0.279
2.1TyrGlu: 2.1 ± 0.188
1.661TyrPhe: 1.661 ± 0.144
2.817TyrGly: 2.817 ± 0.222
0.961TyrHis: 0.961 ± 0.165
2.654TyrIle: 2.654 ± 0.192
2.605TyrLys: 2.605 ± 0.265
3.126TyrLeu: 3.126 ± 0.276
0.798TyrMet: 0.798 ± 0.181
2.573TyrAsn: 2.573 ± 0.239
1.873TyrPro: 1.873 ± 0.182
1.612TyrGln: 1.612 ± 0.215
2.622TyrArg: 2.622 ± 0.189
2.768TyrSer: 2.768 ± 0.254
2.915TyrThr: 2.915 ± 0.31
2.996TyrVal: 2.996 ± 0.209
0.537TyrTrp: 0.537 ± 0.119
2.133TyrTyr: 2.133 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 235 proteins (61416 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski