Vernonia crinkle virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L4AB06|A0A1L4AB06_9GEMI Capsid protein OS=Vernonia crinkle virus OX=1925153 GN=AV1 PE=3 SV=1
MM1 pKa = 7.28PRR3 pKa = 11.84SGAFLVNARR12 pKa = 11.84NFFLTYY18 pKa = 9.45PRR20 pKa = 11.84CSLTKK25 pKa = 10.63EE26 pKa = 3.96EE27 pKa = 5.23ALSQLLALDD36 pKa = 3.86TPTNKK41 pKa = 10.06LFIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.34LHH51 pKa = 6.38EE52 pKa = 4.95NGEE55 pKa = 4.28PHH57 pKa = 6.66LHH59 pKa = 6.03VLIQFEE65 pKa = 5.0GKK67 pKa = 9.87FKK69 pKa = 10.83CRR71 pKa = 11.84NNRR74 pKa = 11.84FFDD77 pKa = 4.01LNSPNRR83 pKa = 11.84STQFHH88 pKa = 7.08PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.05VKK101 pKa = 11.21SYY103 pKa = 9.59MEE105 pKa = 4.47KK106 pKa = 10.83DD107 pKa = 3.45GDD109 pKa = 4.06VLDD112 pKa = 4.81HH113 pKa = 7.15GVFQIDD119 pKa = 3.19GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQNANDD132 pKa = 4.62AYY134 pKa = 10.85AEE136 pKa = 4.11ALNSGSKK143 pKa = 9.79SQALDD148 pKa = 3.1ILKK151 pKa = 10.44EE152 pKa = 3.92KK153 pKa = 10.55APKK156 pKa = 10.58DD157 pKa = 3.54FVLQFHH163 pKa = 6.7NLNANLDD170 pKa = 4.27RR171 pKa = 11.84IFAPPMEE178 pKa = 4.48QYY180 pKa = 10.62ISPFLSSSFNNVPDD194 pKa = 4.15TLEE197 pKa = 3.72EE198 pKa = 3.96WASEE202 pKa = 4.27NVCAAAARR210 pKa = 11.84PIRR213 pKa = 11.84PLSLVLEE220 pKa = 4.7GDD222 pKa = 3.39SRR224 pKa = 11.84TGKK227 pKa = 7.64TLWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.37LDD245 pKa = 4.41LSPKK249 pKa = 10.0IYY251 pKa = 11.02SNDD254 pKa = 2.5AWFNIIDD261 pKa = 4.53DD262 pKa = 4.12VDD264 pKa = 3.5PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.31KK312 pKa = 10.67EE313 pKa = 3.89FLDD316 pKa = 3.9EE317 pKa = 4.62EE318 pKa = 4.57KK319 pKa = 11.37NNALKK324 pKa = 10.74YY325 pKa = 9.06WAIKK329 pKa = 9.82NALFHH334 pKa = 6.9TITEE338 pKa = 4.33PLFSTEE344 pKa = 4.22HH345 pKa = 5.37QSPTQRR351 pKa = 11.84EE352 pKa = 4.18EE353 pKa = 4.45TEE355 pKa = 4.23VNTPEE360 pKa = 3.92EE361 pKa = 4.28DD362 pKa = 3.14

Molecular weight: 41.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L4AB12|A0A1L4AB12_9GEMI AC4 protein OS=Vernonia crinkle virus OX=1925153 GN=AC4 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.28RR3 pKa = 11.84PINIPISSPSLVSKK17 pKa = 9.91VRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84LNFDD25 pKa = 3.27SPASARR31 pKa = 11.84PISPYY36 pKa = 9.95VRR38 pKa = 11.84SARR41 pKa = 11.84AQWQNRR47 pKa = 11.84PMFRR51 pKa = 11.84KK52 pKa = 8.63PRR54 pKa = 11.84IYY56 pKa = 10.8RR57 pKa = 11.84MYY59 pKa = 10.18RR60 pKa = 11.84SSDD63 pKa = 3.49VPRR66 pKa = 11.84GCEE69 pKa = 4.35GPCKK73 pKa = 9.97IQSFDD78 pKa = 3.31QRR80 pKa = 11.84DD81 pKa = 4.22SVVHH85 pKa = 5.94TGNVRR90 pKa = 11.84CLSDD94 pKa = 3.27VTRR97 pKa = 11.84GSGLTHH103 pKa = 5.79RR104 pKa = 11.84TGKK107 pKa = 10.03RR108 pKa = 11.84LCIKK112 pKa = 10.18SIYY115 pKa = 9.82IIGKK119 pKa = 8.07IWMDD123 pKa = 3.54EE124 pKa = 3.93NVKK127 pKa = 10.47KK128 pKa = 10.93SNHH131 pKa = 5.83TNTCMFWLVRR141 pKa = 11.84DD142 pKa = 3.7RR143 pKa = 11.84RR144 pKa = 11.84PYY146 pKa = 8.77GTSPQDD152 pKa = 3.2FGQVFNMFDD161 pKa = 3.99NEE163 pKa = 4.11PSTATVKK170 pKa = 10.93NDD172 pKa = 2.72MRR174 pKa = 11.84DD175 pKa = 3.1RR176 pKa = 11.84YY177 pKa = 10.0QVLRR181 pKa = 11.84KK182 pKa = 9.71FKK184 pKa = 9.46VTVTGGPYY192 pKa = 9.91ACKK195 pKa = 10.18EE196 pKa = 3.93SAIVNKK202 pKa = 9.7FLNLYY207 pKa = 9.71HH208 pKa = 6.71HH209 pKa = 6.4VTYY212 pKa = 10.97NHH214 pKa = 5.96QEE216 pKa = 3.49AAKK219 pKa = 10.4YY220 pKa = 9.25EE221 pKa = 4.13NHH223 pKa = 6.46TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 10.67ASLKK246 pKa = 8.7IRR248 pKa = 11.84MYY250 pKa = 10.72FYY252 pKa = 11.21DD253 pKa = 3.56SVTNN257 pKa = 3.98

Molecular weight: 29.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1125

99

362

187.5

21.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.333 ± 0.583

2.311 ± 0.286

4.533 ± 0.566

5.244 ± 0.841

4.711 ± 0.73

4.356 ± 0.453

4.356 ± 0.708

5.778 ± 0.869

5.333 ± 0.503

8.0 ± 0.893

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.489 ± 0.771

6.756 ± 0.61

6.222 ± 0.56

4.533 ± 0.672

6.489 ± 0.912

8.178 ± 1.3

6.044 ± 0.805

4.533 ± 0.874

1.244 ± 0.192

3.556 ± 0.565
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski