Hydrangea ringspot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5DKR0|Q5DKR0_9VIRU Movement protein TGBp3 OS=Hydrangea ringspot virus OX=112228 GN=TGBp3 PE=3 SV=1
MM1 pKa = 6.92TTTTTTNPIPPQAGTSTPSAAFAPLSQEE29 pKa = 3.98VLEE32 pKa = 4.37TLSFPVTSNLVPSPTEE48 pKa = 3.41LAAIADD54 pKa = 3.76AWTTLGVPAAEE65 pKa = 4.39TTKK68 pKa = 10.7HH69 pKa = 6.18ALALVNFCFDD79 pKa = 3.81SGSSATTRR87 pKa = 11.84FTGASPTPTIPLSALAGAVLEE108 pKa = 4.43LVPIRR113 pKa = 11.84KK114 pKa = 8.36FCRR117 pKa = 11.84YY118 pKa = 7.69FAQYY122 pKa = 9.06IWNARR127 pKa = 11.84LTANAPPASWEE138 pKa = 3.69AWNFPEE144 pKa = 4.32NEE146 pKa = 4.07KK147 pKa = 10.72FAGFDD152 pKa = 3.4FFDD155 pKa = 4.24GVLNTASLKK164 pKa = 10.39PPQGLVRR171 pKa = 11.84KK172 pKa = 7.73PTEE175 pKa = 3.85AEE177 pKa = 3.72RR178 pKa = 11.84VANATAKK185 pKa = 10.4SLHH188 pKa = 6.23LFEE191 pKa = 6.24AATQRR196 pKa = 11.84SNLASTATQFTRR208 pKa = 11.84GRR210 pKa = 11.84LTDD213 pKa = 3.76TSPTIQFLPAPEE225 pKa = 3.76

Molecular weight: 23.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5DKQ9|Q5DKQ9_9VIRU Coat protein OS=Hydrangea ringspot virus OX=112228 PE=3 SV=1
MM1 pKa = 7.98PGPPSEE7 pKa = 4.25SRR9 pKa = 11.84RR10 pKa = 11.84QKK12 pKa = 10.68RR13 pKa = 11.84PSTPSLWSTSVLTRR27 pKa = 11.84ALPQPPASLEE37 pKa = 4.07PPPPPPSPCPPWPEE51 pKa = 4.63PFWSWYY57 pKa = 8.93PSEE60 pKa = 4.93SSVGTSPSTSGMLASPPTPPRR81 pKa = 11.84LHH83 pKa = 6.47GKK85 pKa = 10.04LGISQRR91 pKa = 11.84TRR93 pKa = 11.84SLPASISSMGSSTRR107 pKa = 11.84PPSSHH112 pKa = 6.7PRR114 pKa = 11.84GSSGSPRR121 pKa = 11.84KK122 pKa = 9.87QNGSPTQPPSPSTSLRR138 pKa = 11.84QPPSAPTSRR147 pKa = 11.84PPPPSLPVGDD157 pKa = 4.15

Molecular weight: 16.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2187

73

1386

364.5

40.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.871 ± 1.467

1.463 ± 0.277

3.429 ± 0.558

5.761 ± 1.447

4.024 ± 0.737

5.03 ± 0.322

3.292 ± 0.633

4.115 ± 0.805

5.121 ± 1.472

11.248 ± 0.966

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.738 ± 0.506

2.743 ± 0.505

9.968 ± 3.142

4.024 ± 0.617

5.213 ± 0.634

7.819 ± 2.655

7.727 ± 1.186

4.435 ± 0.451

1.189 ± 0.323

2.789 ± 0.785
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski