Branchiostoma belcheri (Amphioxus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Cephalochordata; Leptocardii; Amphioxiformes; Branchiostomidae; Branchiostoma

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P4ZVL0|A0A6P4ZVL0_BRABE probable G-protein coupled receptor 139 OS=Branchiostoma belcheri OX=7741 GN=LOC109480299 PE=4 SV=1
MM1 pKa = 7.3TPILLFFVAVATAAPMRR18 pKa = 11.84RR19 pKa = 11.84QTRR22 pKa = 11.84ILDD25 pKa = 3.69ANGDD29 pKa = 3.97GLISKK34 pKa = 10.1PEE36 pKa = 3.94VTSVMTLHH44 pKa = 7.2DD45 pKa = 4.67ALVALDD51 pKa = 3.44IDD53 pKa = 4.47GDD55 pKa = 3.98HH56 pKa = 7.04FLYY59 pKa = 10.62LPQIVEE65 pKa = 4.15LFGDD69 pKa = 4.31GSIFYY74 pKa = 10.42LLNSNGDD81 pKa = 3.46DD82 pKa = 3.18HH83 pKa = 8.81LSFGEE88 pKa = 4.28VQHH91 pKa = 6.62GLTLSEE97 pKa = 4.94FFDD100 pKa = 5.15LFDD103 pKa = 4.68KK104 pKa = 11.56NGDD107 pKa = 3.68GVLDD111 pKa = 3.81TSEE114 pKa = 4.57SYY116 pKa = 10.57QMNYY120 pKa = 9.53IYY122 pKa = 10.04NTIQNPDD129 pKa = 3.33AAITNALDD137 pKa = 3.58ANGDD141 pKa = 3.72GKK143 pKa = 10.94LSKK146 pKa = 11.09LEE148 pKa = 3.86VLGAMTLEE156 pKa = 4.12EE157 pKa = 4.79SMIAMDD163 pKa = 4.59ADD165 pKa = 3.57GDD167 pKa = 4.26GFFTVQEE174 pKa = 4.06LMPIFGDD181 pKa = 3.62VTQATSDD188 pKa = 3.63QLDD191 pKa = 3.67TNMDD195 pKa = 3.61GLLSFDD201 pKa = 3.48EE202 pKa = 4.73VTFGTSLDD210 pKa = 4.24RR211 pKa = 11.84IFDD214 pKa = 4.35FYY216 pKa = 11.77DD217 pKa = 3.92LDD219 pKa = 5.3GDD221 pKa = 4.43GYY223 pKa = 10.05LTGSEE228 pKa = 4.27ADD230 pKa = 3.84GIYY233 pKa = 10.47YY234 pKa = 10.39VYY236 pKa = 10.01TAIVNNPILVHH247 pKa = 6.55GGLDD251 pKa = 3.43ADD253 pKa = 4.31GDD255 pKa = 4.45GKK257 pKa = 11.21LSLPEE262 pKa = 3.92VEE264 pKa = 4.5LAMNIHH270 pKa = 7.06DD271 pKa = 5.33ALLALDD277 pKa = 3.75QDD279 pKa = 4.54GDD281 pKa = 3.54NHH283 pKa = 7.48LNEE286 pKa = 4.33QEE288 pKa = 4.12FVHH291 pKa = 6.19YY292 pKa = 10.97VGDD295 pKa = 3.41QLLFHH300 pKa = 6.52TLDD303 pKa = 3.56HH304 pKa = 6.89NGDD307 pKa = 3.34QTLSFGEE314 pKa = 4.11MHH316 pKa = 6.89NLGLDD321 pKa = 3.62TLFNQYY327 pKa = 10.61DD328 pKa = 3.72KK329 pKa = 11.72NGDD332 pKa = 3.49GFLEE336 pKa = 4.33GLEE339 pKa = 4.43AEE341 pKa = 4.75TMLHH345 pKa = 6.8VYY347 pKa = 10.79DD348 pKa = 5.47LVQAMNAAA356 pKa = 3.88

Molecular weight: 39.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P4YBX3|A0A6P4YBX3_BRABE uncharacterized protein LOC109463859 OS=Branchiostoma belcheri OX=7741 GN=LOC109463859 PE=4 SV=1
MM1 pKa = 7.85PKK3 pKa = 9.97RR4 pKa = 11.84ARR6 pKa = 11.84SASGSRR12 pKa = 11.84KK13 pKa = 8.78PAAKK17 pKa = 9.68KK18 pKa = 10.23AKK20 pKa = 9.4PAAKK24 pKa = 9.78RR25 pKa = 11.84KK26 pKa = 9.97APAAAAAAAPPAKK39 pKa = 9.99KK40 pKa = 10.1ARR42 pKa = 11.84PAKK45 pKa = 10.15AKK47 pKa = 9.89KK48 pKa = 10.24APRR51 pKa = 11.84KK52 pKa = 9.34KK53 pKa = 10.0AAKK56 pKa = 9.52PRR58 pKa = 11.84AKK60 pKa = 9.77PAKK63 pKa = 9.95KK64 pKa = 10.14SFKK67 pKa = 10.28KK68 pKa = 10.32KK69 pKa = 10.07AGGRR73 pKa = 11.84KK74 pKa = 8.99KK75 pKa = 10.69

Molecular weight: 7.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23850

7764

31614

20550264

54

24062

650.0

72.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.859 ± 0.013

2.307 ± 0.022

5.962 ± 0.017

6.821 ± 0.021

3.316 ± 0.01

6.805 ± 0.023

2.411 ± 0.008

4.133 ± 0.01

5.46 ± 0.02

8.317 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.007

3.996 ± 0.012

5.733 ± 0.018

4.741 ± 0.018

5.631 ± 0.013

7.759 ± 0.015

6.753 ± 0.03

6.582 ± 0.012

1.175 ± 0.004

2.85 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski