Enterococcus saccharolyticus subsp. saccharolyticus ATCC 43076

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus saccharolyticus; Enterococcus saccharolyticus subsp. saccharolyticus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0NS35|S0NS35_9ENTE Oxidoreductase NAD-binding Rossmann fold protein OS=Enterococcus saccharolyticus subsp. saccharolyticus ATCC 43076 OX=1139996 GN=OMQ_00875 PE=4 SV=1
MM1 pKa = 7.48TEE3 pKa = 3.83HH4 pKa = 5.93THH6 pKa = 5.77DD7 pKa = 4.4HH8 pKa = 5.01NHH10 pKa = 6.65EE11 pKa = 4.13GHH13 pKa = 5.46EE14 pKa = 4.62HH15 pKa = 5.39ITLVDD20 pKa = 3.67DD21 pKa = 4.08QGNEE25 pKa = 3.72TLYY28 pKa = 10.95EE29 pKa = 3.99ILLTIDD35 pKa = 3.44GEE37 pKa = 4.66EE38 pKa = 4.06EE39 pKa = 3.67FGRR42 pKa = 11.84NYY44 pKa = 10.57VLLYY48 pKa = 9.37PAGVPEE54 pKa = 4.43DD55 pKa = 4.2EE56 pKa = 4.41EE57 pKa = 5.21VEE59 pKa = 4.24LQAYY63 pKa = 9.96AYY65 pKa = 10.46VEE67 pKa = 4.18NADD70 pKa = 3.61GTEE73 pKa = 4.07GDD75 pKa = 3.86LEE77 pKa = 4.31EE78 pKa = 4.93IKK80 pKa = 9.86TDD82 pKa = 3.94KK83 pKa = 10.23EE84 pKa = 3.25WDD86 pKa = 3.53MIEE89 pKa = 4.33EE90 pKa = 4.2VFNTFMAEE98 pKa = 3.9EE99 pKa = 4.15EE100 pKa = 4.24

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0JD15|S0JD15_9ENTE Uncharacterized protein OS=Enterococcus saccharolyticus subsp. saccharolyticus ATCC 43076 OX=1139996 GN=OMQ_02245 PE=4 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.15QKK5 pKa = 7.56THH7 pKa = 6.75RR8 pKa = 11.84GLAKK12 pKa = 9.74RR13 pKa = 11.84VKK15 pKa = 8.53RR16 pKa = 11.84TGGGGLKK23 pKa = 9.95RR24 pKa = 11.84ARR26 pKa = 11.84AFTSHH31 pKa = 6.92RR32 pKa = 11.84FHH34 pKa = 7.48GKK36 pKa = 6.37TKK38 pKa = 9.57KK39 pKa = 9.43QRR41 pKa = 11.84RR42 pKa = 11.84QLRR45 pKa = 11.84KK46 pKa = 9.64ARR48 pKa = 11.84MVSSGDD54 pKa = 3.38FKK56 pKa = 11.19RR57 pKa = 11.84IRR59 pKa = 11.84QQLARR64 pKa = 11.84MKK66 pKa = 10.67

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2573

0

2573

761874

30

1494

296.1

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.424 ± 0.056

0.638 ± 0.012

5.166 ± 0.037

7.47 ± 0.044

4.691 ± 0.037

6.463 ± 0.047

1.996 ± 0.022

7.522 ± 0.045

6.329 ± 0.042

9.954 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.023

4.356 ± 0.031

3.48 ± 0.021

4.597 ± 0.046

3.933 ± 0.04

5.584 ± 0.036

6.054 ± 0.036

7.03 ± 0.046

0.928 ± 0.015

3.692 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski