Ophiocordyceps unilateralis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Ophiocordyceps

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A9P7E5|A0A2A9P7E5_9HYPO Uncharacterized protein OS=Ophiocordyceps unilateralis OX=268505 GN=XA68_15485 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.47YY3 pKa = 10.79NSLVLIGLAARR14 pKa = 11.84SSASPQADD22 pKa = 3.14SGAVTAKK29 pKa = 9.99IAPQGGILEE38 pKa = 4.91GCQTTYY44 pKa = 10.58PGRR47 pKa = 11.84FQVRR51 pKa = 11.84TQRR54 pKa = 11.84LSDD57 pKa = 3.51QPLVNQQPQACGADD71 pKa = 3.3KK72 pKa = 11.11SLVMTLNGGVLTDD85 pKa = 3.77GKK87 pKa = 11.28SRR89 pKa = 11.84TGYY92 pKa = 9.57VASNYY97 pKa = 8.49QFQFDD102 pKa = 4.8GPPQSGSIYY111 pKa = 8.98TAGFSLCGNGSLALGSSAVFYY132 pKa = 10.87QCLSGSFYY140 pKa = 11.4NLYY143 pKa = 10.3DD144 pKa = 4.22RR145 pKa = 11.84NWAAQCEE152 pKa = 4.98PILMMAVPCSDD163 pKa = 3.1GSGSGSAADD172 pKa = 4.1IGAGDD177 pKa = 4.11AASSSSQSPGTISQIGDD194 pKa = 3.82GQPQAPVQPQTPNQPGQAPEE214 pKa = 4.18QPVQAPNQPGQTPDD228 pKa = 3.41QPGQAPNQPGQAPEE242 pKa = 4.04QPGQGPNQPQSPVQPGAPVVSQIGDD267 pKa = 3.81GQPQAPVQPPAPVVSEE283 pKa = 4.26INDD286 pKa = 3.95GQPEE290 pKa = 4.24APAQPQTPAQPQAPTQPQAPDD311 pKa = 3.25QPQAPVVSEE320 pKa = 4.21INDD323 pKa = 3.98GQPEE327 pKa = 4.36APVGNAPPAEE337 pKa = 4.3TPATGQTPGGQSPSEE352 pKa = 4.22GGSPSQEE359 pKa = 3.6SSAPSATSAAPSSGQDD375 pKa = 2.99NTQAPEE381 pKa = 3.93ATKK384 pKa = 9.74PAYY387 pKa = 9.41EE388 pKa = 4.41VSQGMQNAASGIATALVFAMAGSFLFLL415 pKa = 4.7

Molecular weight: 41.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A9PI08|A0A2A9PI08_9HYPO Uncharacterized protein OS=Ophiocordyceps unilateralis OX=268505 GN=XA68_18384 PE=4 SV=1
MM1 pKa = 7.64PCSPPLPAEE10 pKa = 4.33GKK12 pKa = 10.43LRR14 pKa = 11.84GHH16 pKa = 7.12DD17 pKa = 3.38KK18 pKa = 10.97ASNSHH23 pKa = 5.63IRR25 pKa = 11.84RR26 pKa = 11.84DD27 pKa = 3.61AIIPLRR33 pKa = 11.84QVARR37 pKa = 11.84RR38 pKa = 11.84NHH40 pKa = 5.18RR41 pKa = 11.84TGFFPVLARR50 pKa = 11.84VVYY53 pKa = 10.0SRR55 pKa = 11.84SVLALRR61 pKa = 11.84LGFCYY66 pKa = 10.3CPNVSALDD74 pKa = 3.77LLIGRR79 pKa = 11.84KK80 pKa = 8.86LWLSRR85 pKa = 11.84VGWPLCCKK93 pKa = 10.22LNNGLHH99 pKa = 5.94VGVLDD104 pKa = 4.62DD105 pKa = 4.89SSRR108 pKa = 11.84LPPLSKK114 pKa = 9.73RR115 pKa = 11.84TLIGTSASVTVEE127 pKa = 3.78KK128 pKa = 10.73KK129 pKa = 8.46HH130 pKa = 5.07THH132 pKa = 3.82THH134 pKa = 5.81TYY136 pKa = 9.78

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8572

0

8572

4052776

66

7725

472.8

52.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.629 ± 0.027

1.342 ± 0.012

6.126 ± 0.02

5.789 ± 0.029

3.403 ± 0.017

7.124 ± 0.025

2.474 ± 0.012

3.907 ± 0.02

4.317 ± 0.023

9.073 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.009

3.039 ± 0.014

6.409 ± 0.032

3.987 ± 0.021

7.238 ± 0.024

8.291 ± 0.033

5.46 ± 0.017

6.366 ± 0.019

1.385 ± 0.011

2.374 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski