Rhizobium sp. AC44/96

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5685 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B9SIG0|A0A1B9SIG0_9RHIZ Protein-glutamate methylesterase/protein-glutamine glutaminase OS=Rhizobium sp. AC44/96 OX=1841654 GN=cheB PE=3 SV=1
MM1 pKa = 7.17PQSILNAVGEE11 pKa = 4.28SLYY14 pKa = 11.09YY15 pKa = 10.62SGGSSAWFSATGSGPILNGTAGNDD39 pKa = 4.2SIWGDD44 pKa = 3.38SSVNVTMQGGTGDD57 pKa = 4.56DD58 pKa = 2.86IYY60 pKa = 11.71YY61 pKa = 10.19LYY63 pKa = 11.17SSINRR68 pKa = 11.84AVEE71 pKa = 3.86APGAGVDD78 pKa = 4.95TINTWMSYY86 pKa = 7.27TLPEE90 pKa = 4.06NFEE93 pKa = 4.33NLTVTGNGRR102 pKa = 11.84YY103 pKa = 9.82AFGNAADD110 pKa = 4.65NIISGASGSQTIDD123 pKa = 2.67GGAGNDD129 pKa = 3.49VLIGGGGADD138 pKa = 2.98TFIFTRR144 pKa = 11.84GNGSDD149 pKa = 4.56LIVDD153 pKa = 4.72FSSDD157 pKa = 3.18DD158 pKa = 3.82TIRR161 pKa = 11.84LDD163 pKa = 4.13GYY165 pKa = 11.09GLTSFDD171 pKa = 3.45QVLSHH176 pKa = 6.4STQEE180 pKa = 4.23GNNLRR185 pKa = 11.84LDD187 pKa = 3.84LGGGEE192 pKa = 4.46SLVLANHH199 pKa = 6.31TVADD203 pKa = 3.85LHH205 pKa = 7.07SDD207 pKa = 3.66QFQLTLDD214 pKa = 3.87RR215 pKa = 11.84SNLTLTFADD224 pKa = 4.17EE225 pKa = 4.44FDD227 pKa = 4.07SLSLRR232 pKa = 11.84NGDD235 pKa = 3.74QGTWDD240 pKa = 3.74AKK242 pKa = 10.44FWWAPDD248 pKa = 3.41KK249 pKa = 11.45GSTLPGNGEE258 pKa = 4.03QQWYY262 pKa = 9.82INPSYY267 pKa = 11.08AGTSAVNPFSVDD279 pKa = 3.12NGVLTITANKK289 pKa = 8.51TPAALEE295 pKa = 4.14SQIDD299 pKa = 4.45GYY301 pKa = 11.23DD302 pKa = 3.51YY303 pKa = 11.28TSGILTTHH311 pKa = 6.54SSFAQTYY318 pKa = 8.52GYY320 pKa = 10.77FEE322 pKa = 4.65IRR324 pKa = 11.84ADD326 pKa = 3.51MPTEE330 pKa = 3.84QGAWPAFWLLPEE342 pKa = 5.2DD343 pKa = 4.82GSWPPEE349 pKa = 3.78LDD351 pKa = 3.52VVEE354 pKa = 5.08MRR356 pKa = 11.84GQDD359 pKa = 3.42PKK361 pKa = 10.12TVNVTVHH368 pKa = 5.88SADD371 pKa = 3.39GGTHH375 pKa = 5.35VMEE378 pKa = 4.28STPVKK383 pKa = 10.02VASTEE388 pKa = 4.04GFHH391 pKa = 6.6TYY393 pKa = 9.81GVLWDD398 pKa = 3.65EE399 pKa = 4.83DD400 pKa = 3.88QIVWYY405 pKa = 10.0FDD407 pKa = 3.66DD408 pKa = 3.85VAVAHH413 pKa = 7.24ADD415 pKa = 3.88TPADD419 pKa = 3.52MHH421 pKa = 7.3DD422 pKa = 3.72PMYY425 pKa = 10.39MLVNLAIGGTAGTPTDD441 pKa = 3.75GLPNGSEE448 pKa = 3.7FHH450 pKa = 6.86IDD452 pKa = 3.47YY453 pKa = 10.66IHH455 pKa = 7.31AYY457 pKa = 9.62SLADD461 pKa = 3.63SPVTQATHH469 pKa = 6.92AATDD473 pKa = 3.31DD474 pKa = 3.59WQAA477 pKa = 3.04

Molecular weight: 50.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B9SMJ5|A0A1B9SMJ5_9RHIZ YCII domain-containing protein OS=Rhizobium sp. AC44/96 OX=1841654 GN=A6U87_00840 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.1GGRR28 pKa = 11.84KK29 pKa = 9.61VIVARR34 pKa = 11.84RR35 pKa = 11.84GRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.08RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5685

0

5685

1779321

29

4176

313.0

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.847 ± 0.042

0.794 ± 0.01

5.742 ± 0.034

5.601 ± 0.029

3.955 ± 0.024

8.246 ± 0.034

2.004 ± 0.015

5.767 ± 0.023

3.803 ± 0.028

9.878 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.016

2.939 ± 0.02

4.775 ± 0.024

3.158 ± 0.019

6.422 ± 0.036

5.982 ± 0.027

5.454 ± 0.032

7.443 ± 0.025

1.279 ± 0.013

2.363 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski