Bluetongue virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5IWW5|C5IWW5_9REOV VP6 OS=Bluetongue virus OX=40051 PE=4 SV=1
MM1 pKa = 7.04EE2 pKa = 4.1QKK4 pKa = 9.85QRR6 pKa = 11.84RR7 pKa = 11.84FTKK10 pKa = 10.67NIFVLDD16 pKa = 4.16ANGKK20 pKa = 4.68TLCGAIAKK28 pKa = 9.92LSSQPYY34 pKa = 8.05CQIKK38 pKa = 9.15IGRR41 pKa = 11.84VIAFKK46 pKa = 10.12PVKK49 pKa = 10.12NPEE52 pKa = 3.87PKK54 pKa = 10.38GYY56 pKa = 10.08VLNVPGPGAYY66 pKa = 9.6RR67 pKa = 11.84IQDD70 pKa = 3.57GQDD73 pKa = 3.18IISLMLTPHH82 pKa = 5.88GVEE85 pKa = 3.73ATTEE89 pKa = 3.8RR90 pKa = 11.84WEE92 pKa = 3.82EE93 pKa = 3.85WKK95 pKa = 11.01FEE97 pKa = 4.21GVSVTPMATRR107 pKa = 11.84VQHH110 pKa = 5.97NGVMVDD116 pKa = 5.38AEE118 pKa = 4.1IKK120 pKa = 8.19YY121 pKa = 9.36CKK123 pKa = 10.33GMGIVQPYY131 pKa = 8.14MRR133 pKa = 11.84NDD135 pKa = 3.34FDD137 pKa = 5.32RR138 pKa = 11.84NEE140 pKa = 4.16MPDD143 pKa = 3.43LPGVMRR149 pKa = 11.84SNYY152 pKa = 9.42DD153 pKa = 3.28VRR155 pKa = 11.84EE156 pKa = 3.94LRR158 pKa = 11.84QKK160 pKa = 10.23IKK162 pKa = 10.85NEE164 pKa = 3.89RR165 pKa = 11.84EE166 pKa = 3.82SAPRR170 pKa = 11.84LQVQSVAPRR179 pKa = 11.84EE180 pKa = 4.1EE181 pKa = 4.34SRR183 pKa = 11.84WMDD186 pKa = 3.74DD187 pKa = 4.45DD188 pKa = 3.79EE189 pKa = 6.59AKK191 pKa = 10.56VDD193 pKa = 3.91EE194 pKa = 4.88EE195 pKa = 4.35AKK197 pKa = 10.84EE198 pKa = 4.14MIPGTSRR205 pKa = 11.84LEE207 pKa = 3.96KK208 pKa = 10.49LRR210 pKa = 11.84EE211 pKa = 3.81ARR213 pKa = 11.84SNVFKK218 pKa = 10.71EE219 pKa = 4.2VEE221 pKa = 4.44AGINWNLDD229 pKa = 3.37EE230 pKa = 5.9KK231 pKa = 11.28DD232 pKa = 3.5EE233 pKa = 4.18EE234 pKa = 4.5DD235 pKa = 3.59RR236 pKa = 11.84DD237 pKa = 3.75EE238 pKa = 6.26RR239 pKa = 11.84EE240 pKa = 4.62DD241 pKa = 3.73EE242 pKa = 4.34EE243 pKa = 4.58QVKK246 pKa = 8.3TLSDD250 pKa = 3.89DD251 pKa = 4.21DD252 pKa = 4.27EE253 pKa = 4.9QGEE256 pKa = 4.2DD257 pKa = 4.05ASDD260 pKa = 3.95DD261 pKa = 3.9EE262 pKa = 4.81HH263 pKa = 6.91PKK265 pKa = 8.39THH267 pKa = 5.79ITKK270 pKa = 10.22EE271 pKa = 4.16YY272 pKa = 8.56IEE274 pKa = 4.81KK275 pKa = 10.13VAKK278 pKa = 9.73QIKK281 pKa = 10.13LKK283 pKa = 10.47DD284 pKa = 3.66EE285 pKa = 4.41RR286 pKa = 11.84FMSLSSAMPQASGGFDD302 pKa = 3.1RR303 pKa = 11.84MIVTKK308 pKa = 10.26KK309 pKa = 10.67LKK311 pKa = 8.0WQNVPLYY318 pKa = 10.72CFDD321 pKa = 4.17EE322 pKa = 4.26SSKK325 pKa = 10.62RR326 pKa = 11.84YY327 pKa = 8.95EE328 pKa = 4.11LQCVGACEE336 pKa = 3.83RR337 pKa = 11.84VAFVSKK343 pKa = 10.81DD344 pKa = 3.14MSLIILPVGVV354 pKa = 3.86

Molecular weight: 40.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9YIN8|Q9YIN8_9REOV Non-structural protein NS3 OS=Bluetongue virus OX=40051 GN=NS3 PE=3 SV=1
MM1 pKa = 7.7LSGLIQRR8 pKa = 11.84FEE10 pKa = 4.13EE11 pKa = 4.25EE12 pKa = 4.3KK13 pKa = 10.19MKK15 pKa = 10.81HH16 pKa = 4.03NQEE19 pKa = 4.06RR20 pKa = 11.84VEE22 pKa = 4.09EE23 pKa = 4.12LSLVRR28 pKa = 11.84VDD30 pKa = 4.55DD31 pKa = 5.04TISQPPRR38 pKa = 11.84YY39 pKa = 9.29APSAPMPSSMPTVALEE55 pKa = 4.24ILDD58 pKa = 3.75KK59 pKa = 11.5AMSNTTGATQTQKK72 pKa = 11.26AEE74 pKa = 3.9KK75 pKa = 10.12AAFASYY81 pKa = 11.37AEE83 pKa = 4.24AFRR86 pKa = 11.84DD87 pKa = 4.13DD88 pKa = 3.2VRR90 pKa = 11.84LRR92 pKa = 11.84QIKK95 pKa = 9.78RR96 pKa = 11.84HH97 pKa = 4.98VNEE100 pKa = 4.23QILPKK105 pKa = 10.44LKK107 pKa = 10.51SDD109 pKa = 4.47LGGLKK114 pKa = 9.95KK115 pKa = 10.16KK116 pKa = 10.37RR117 pKa = 11.84AIIHH121 pKa = 4.9MTLLIAAVVALLTSVCTLSSDD142 pKa = 3.16MSVAFKK148 pKa = 11.33LNGTSAEE155 pKa = 3.85IPQWFKK161 pKa = 11.52SLNPMLGVVNLGATFLMMVCAKK183 pKa = 9.83SEE185 pKa = 3.92RR186 pKa = 11.84SLNQQIDD193 pKa = 4.05MIKK196 pKa = 10.21KK197 pKa = 9.45EE198 pKa = 4.14VMKK201 pKa = 10.61KK202 pKa = 8.85QSYY205 pKa = 9.19NDD207 pKa = 3.56AVRR210 pKa = 11.84MSFTEE215 pKa = 4.85FSSVPLDD222 pKa = 3.74GFEE225 pKa = 5.49LPLTT229 pKa = 4.16

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6147

229

1302

614.7

70.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.995 ± 0.504

1.057 ± 0.213

6.117 ± 0.3

7.304 ± 0.519

3.921 ± 0.315

5.629 ± 0.373

2.261 ± 0.289

6.93 ± 0.315

5.759 ± 0.637

8.378 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.709 ± 0.267

3.921 ± 0.201

4.262 ± 0.282

3.774 ± 0.357

7.142 ± 0.265

5.58 ± 0.386

5.141 ± 0.443

6.93 ± 0.22

1.285 ± 0.207

3.904 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski