Amino acid dipepetide frequency for Pseudomethylobacillus aquaticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.889AlaAla: 13.889 ± 0.203	1.088AlaCys: 1.088 ± 0.041	6.312AlaAsp: 6.312 ± 0.1	6.614AlaGlu: 6.614 ± 0.127	3.426AlaPhe: 3.426 ± 0.064	8.754AlaGly: 8.754 ± 0.141	2.449AlaHis: 2.449 ± 0.065	5.729AlaIle: 5.729 ± 0.089	3.867AlaLys: 3.867 ± 0.088	13.048AlaLeu: 13.048 ± 0.16	3.193AlaMet: 3.193 ± 0.067	3.328AlaAsn: 3.328 ± 0.084	4.272AlaPro: 4.272 ± 0.091	4.995AlaGln: 4.995 ± 0.091	6.525AlaArg: 6.525 ± 0.105	6.912AlaSer: 6.912 ± 0.107	5.231AlaThr: 5.231 ± 0.084	7.563AlaVal: 7.563 ± 0.13	1.499AlaTrp: 1.499 ± 0.054	2.785AlaTyr: 2.785 ± 0.07	0.0AlaXaa: 0.0 ± 0.0
Cys	0.921CysAla: 0.921 ± 0.032	0.126CysCys: 0.126 ± 0.014	0.463CysAsp: 0.463 ± 0.026	0.471CysGlu: 0.471 ± 0.029	0.279CysPhe: 0.279 ± 0.018	0.863CysGly: 0.863 ± 0.032	0.254CysHis: 0.254 ± 0.019	0.412CysIle: 0.412 ± 0.023	0.311CysLys: 0.311 ± 0.021	0.892CysLeu: 0.892 ± 0.039	0.253CysMet: 0.253 ± 0.018	0.329CysAsn: 0.329 ± 0.021	0.471CysPro: 0.471 ± 0.024	0.303CysGln: 0.303 ± 0.019	0.498CysArg: 0.498 ± 0.026	0.581CysSer: 0.581 ± 0.027	0.45CysThr: 0.45 ± 0.024	0.611CysVal: 0.611 ± 0.026	0.101CysTrp: 0.101 ± 0.014	0.266CysTyr: 0.266 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	6.192AspAla: 6.192 ± 0.124	0.471AspCys: 0.471 ± 0.026	2.927AspAsp: 2.927 ± 0.076	3.049AspGlu: 3.049 ± 0.074	2.166AspPhe: 2.166 ± 0.058	4.311AspGly: 4.311 ± 0.085	1.129AspHis: 1.129 ± 0.038	3.277AspIle: 3.277 ± 0.073	2.282AspLys: 2.282 ± 0.05	5.316AspLeu: 5.316 ± 0.085	1.411AspMet: 1.411 ± 0.047	1.676AspAsn: 1.676 ± 0.048	2.502AspPro: 2.502 ± 0.064	2.157AspGln: 2.157 ± 0.052	2.765AspArg: 2.765 ± 0.062	2.946AspSer: 2.946 ± 0.071	2.831AspThr: 2.831 ± 0.076	3.826AspVal: 3.826 ± 0.074	1.0AspTrp: 1.0 ± 0.034	1.909AspTyr: 1.909 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	6.379GluAla: 6.379 ± 0.107	0.359GluCys: 0.359 ± 0.021	2.478GluAsp: 2.478 ± 0.062	2.599GluGlu: 2.599 ± 0.072	1.97GluPhe: 1.97 ± 0.057	3.163GluGly: 3.163 ± 0.063	1.486GluHis: 1.486 ± 0.04	3.144GluIle: 3.144 ± 0.071	2.367GluLys: 2.367 ± 0.069	5.948GluLeu: 5.948 ± 0.103	1.391GluMet: 1.391 ± 0.04	1.555GluAsn: 1.555 ± 0.051	2.111GluPro: 2.111 ± 0.051	3.432GluGln: 3.432 ± 0.086	3.736GluArg: 3.736 ± 0.087	2.645GluSer: 2.645 ± 0.062	2.76GluThr: 2.76 ± 0.06	4.163GluVal: 4.163 ± 0.08	0.633GluTrp: 0.633 ± 0.035	1.36GluTyr: 1.36 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.485PheAla: 3.485 ± 0.081	0.379PheCys: 0.379 ± 0.024	2.464PheAsp: 2.464 ± 0.053	1.992PheGlu: 1.992 ± 0.057	1.327PhePhe: 1.327 ± 0.047	3.125PheGly: 3.125 ± 0.076	0.74PheHis: 0.74 ± 0.028	1.97PheIle: 1.97 ± 0.05	1.374PheLys: 1.374 ± 0.05	2.986PheLeu: 2.986 ± 0.072	0.965PheMet: 0.965 ± 0.036	1.42PheAsn: 1.42 ± 0.045	1.433PhePro: 1.433 ± 0.047	1.155PheGln: 1.155 ± 0.037	1.806PheArg: 1.806 ± 0.053	2.611PheSer: 2.611 ± 0.065	1.849PheThr: 1.849 ± 0.064	2.565PheVal: 2.565 ± 0.062	0.455PheTrp: 0.455 ± 0.026	0.998PheTyr: 0.998 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	6.965GlyAla: 6.965 ± 0.11	0.761GlyCys: 0.761 ± 0.032	3.897GlyAsp: 3.897 ± 0.082	4.112GlyGlu: 4.112 ± 0.078	3.003GlyPhe: 3.003 ± 0.065	5.823GlyGly: 5.823 ± 0.13	1.876GlyHis: 1.876 ± 0.045	4.53GlyIle: 4.53 ± 0.081	3.862GlyLys: 3.862 ± 0.082	8.206GlyLeu: 8.206 ± 0.142	2.339GlyMet: 2.339 ± 0.055	2.738GlyAsn: 2.738 ± 0.119	2.185GlyPro: 2.185 ± 0.062	3.206GlyGln: 3.206 ± 0.073	4.281GlyArg: 4.281 ± 0.085	4.471GlySer: 4.471 ± 0.088	3.788GlyThr: 3.788 ± 0.093	5.675GlyVal: 5.675 ± 0.091	1.234GlyTrp: 1.234 ± 0.048	2.339GlyTyr: 2.339 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	2.939HisAla: 2.939 ± 0.066	0.269HisCys: 0.269 ± 0.02	1.33HisAsp: 1.33 ± 0.04	1.178HisGlu: 1.178 ± 0.041	0.91HisPhe: 0.91 ± 0.035	2.04HisGly: 2.04 ± 0.05	0.816HisHis: 0.816 ± 0.032	1.298HisIle: 1.298 ± 0.043	0.816HisLys: 0.816 ± 0.036	2.487HisLeu: 2.487 ± 0.067	0.521HisMet: 0.521 ± 0.026	0.677HisAsn: 0.677 ± 0.027	1.315HisPro: 1.315 ± 0.044	1.094HisGln: 1.094 ± 0.044	1.233HisArg: 1.233 ± 0.044	1.292HisSer: 1.292 ± 0.047	1.138HisThr: 1.138 ± 0.037	1.587HisVal: 1.587 ± 0.046	0.454HisTrp: 0.454 ± 0.026	0.926HisTyr: 0.926 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	6.653IleAla: 6.653 ± 0.097	0.445IleCys: 0.445 ± 0.023	3.26IleAsp: 3.26 ± 0.073	3.352IleGlu: 3.352 ± 0.069	1.553IlePhe: 1.553 ± 0.055	4.256IleGly: 4.256 ± 0.077	1.113IleHis: 1.113 ± 0.04	2.434IleIle: 2.434 ± 0.061	2.354IleLys: 2.354 ± 0.06	4.443IleLeu: 4.443 ± 0.098	1.058IleMet: 1.058 ± 0.043	2.095IleAsn: 2.095 ± 0.056	2.456IlePro: 2.456 ± 0.059	1.941IleGln: 1.941 ± 0.057	2.963IleArg: 2.963 ± 0.065	3.601IleSer: 3.601 ± 0.063	3.054IleThr: 3.054 ± 0.079	3.589IleVal: 3.589 ± 0.071	0.493IleTrp: 0.493 ± 0.024	1.293IleTyr: 1.293 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.121LysAla: 4.121 ± 0.082	0.227LysCys: 0.227 ± 0.018	1.848LysAsp: 1.848 ± 0.053	1.796LysGlu: 1.796 ± 0.056	1.155LysPhe: 1.155 ± 0.038	2.423LysGly: 2.423 ± 0.067	1.002LysHis: 1.002 ± 0.041	1.932LysIle: 1.932 ± 0.057	1.654LysLys: 1.654 ± 0.055	4.785LysLeu: 4.785 ± 0.087	0.926LysMet: 0.926 ± 0.04	1.216LysAsn: 1.216 ± 0.048	2.505LysPro: 2.505 ± 0.071	2.169LysGln: 2.169 ± 0.059	2.468LysArg: 2.468 ± 0.061	2.182LysSer: 2.182 ± 0.059	2.242LysThr: 2.242 ± 0.055	2.953LysVal: 2.953 ± 0.07	0.345LysTrp: 0.345 ± 0.021	0.827LysTyr: 0.827 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	12.667LeuAla: 12.667 ± 0.169	0.993LeuCys: 0.993 ± 0.033	5.956LeuAsp: 5.956 ± 0.102	5.607LeuGlu: 5.607 ± 0.095	3.665LeuPhe: 3.665 ± 0.072	8.127LeuGly: 8.127 ± 0.117	2.695LeuHis: 2.695 ± 0.076	5.443LeuIle: 5.443 ± 0.101	4.302LeuLys: 4.302 ± 0.09	12.77LeuLeu: 12.77 ± 0.215	2.716LeuMet: 2.716 ± 0.073	3.846LeuAsn: 3.846 ± 0.079	5.951LeuPro: 5.951 ± 0.105	5.928LeuGln: 5.928 ± 0.124	6.802LeuArg: 6.802 ± 0.123	6.988LeuSer: 6.988 ± 0.113	5.792LeuThr: 5.792 ± 0.093	7.411LeuVal: 7.411 ± 0.134	1.217LeuTrp: 1.217 ± 0.046	2.426LeuTyr: 2.426 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.769MetAla: 2.769 ± 0.063	0.148MetCys: 0.148 ± 0.016	1.25MetAsp: 1.25 ± 0.042	1.233MetGlu: 1.233 ± 0.044	0.783MetPhe: 0.783 ± 0.033	1.608MetGly: 1.608 ± 0.044	0.62MetHis: 0.62 ± 0.026	1.185MetIle: 1.185 ± 0.042	1.019MetLys: 1.019 ± 0.039	3.179MetLeu: 3.179 ± 0.075	0.682MetMet: 0.682 ± 0.032	0.869MetAsn: 0.869 ± 0.031	1.466MetPro: 1.466 ± 0.044	1.743MetGln: 1.743 ± 0.049	1.819MetArg: 1.819 ± 0.053	1.647MetSer: 1.647 ± 0.046	1.449MetThr: 1.449 ± 0.044	1.711MetVal: 1.711 ± 0.048	0.185MetTrp: 0.185 ± 0.015	0.433MetTyr: 0.433 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.417AsnAla: 3.417 ± 0.069	0.275AsnCys: 0.275 ± 0.02	1.705AsnAsp: 1.705 ± 0.076	1.577AsnGlu: 1.577 ± 0.052	1.078AsnPhe: 1.078 ± 0.039	2.701AsnGly: 2.701 ± 0.073	0.694AsnHis: 0.694 ± 0.033	1.925AsnIle: 1.925 ± 0.051	1.382AsnLys: 1.382 ± 0.04	3.362AsnLeu: 3.362 ± 0.081	0.774AsnMet: 0.774 ± 0.034	1.141AsnAsn: 1.141 ± 0.048	1.966AsnPro: 1.966 ± 0.046	1.479AsnGln: 1.479 ± 0.046	1.777AsnArg: 1.777 ± 0.05	1.81AsnSer: 1.81 ± 0.053	1.932AsnThr: 1.932 ± 0.059	2.22AsnVal: 2.22 ± 0.061	0.448AsnTrp: 0.448 ± 0.023	0.888AsnTyr: 0.888 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	5.822ProAla: 5.822 ± 0.109	0.365ProCys: 0.365 ± 0.025	2.958ProAsp: 2.958 ± 0.06	2.995ProGlu: 2.995 ± 0.067	1.604ProPhe: 1.604 ± 0.051	3.75ProGly: 3.75 ± 0.071	1.098ProHis: 1.098 ± 0.042	1.928ProIle: 1.928 ± 0.05	1.541ProLys: 1.541 ± 0.053	4.803ProLeu: 4.803 ± 0.088	1.132ProMet: 1.132 ± 0.035	1.442ProAsn: 1.442 ± 0.038	1.933ProPro: 1.933 ± 0.07	2.08ProGln: 2.08 ± 0.054	2.111ProArg: 2.111 ± 0.059	2.527ProSer: 2.527 ± 0.057	2.119ProThr: 2.119 ± 0.054	3.946ProVal: 3.946 ± 0.079	0.582ProTrp: 0.582 ± 0.031	1.289ProTyr: 1.289 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	6.204GlnAla: 6.204 ± 0.112	0.372GlnCys: 0.372 ± 0.021	2.22GlnAsp: 2.22 ± 0.054	1.97GlnGlu: 1.97 ± 0.058	1.602GlnPhe: 1.602 ± 0.043	3.159GlnGly: 3.159 ± 0.063	1.677GlnHis: 1.677 ± 0.06	2.383GlnIle: 2.383 ± 0.053	1.486GlnLys: 1.486 ± 0.048	5.742GlnLeu: 5.742 ± 0.124	1.146GlnMet: 1.146 ± 0.043	1.174GlnAsn: 1.174 ± 0.044	2.355GlnPro: 2.355 ± 0.069	3.712GlnGln: 3.712 ± 0.119	3.696GlnArg: 3.696 ± 0.082	2.413GlnSer: 2.413 ± 0.063	2.28GlnThr: 2.28 ± 0.056	3.35GlnVal: 3.35 ± 0.079	0.589GlnTrp: 0.589 ± 0.029	1.205GlnTyr: 1.205 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	5.645ArgAla: 5.645 ± 0.1	0.531ArgCys: 0.531 ± 0.028	3.348ArgAsp: 3.348 ± 0.064	3.51ArgGlu: 3.51 ± 0.081	2.468ArgPhe: 2.468 ± 0.056	3.734ArgGly: 3.734 ± 0.079	1.627ArgHis: 1.627 ± 0.049	3.626ArgIle: 3.626 ± 0.069	2.189ArgLys: 2.189 ± 0.057	7.01ArgLeu: 7.01 ± 0.111	1.772ArgMet: 1.772 ± 0.047	1.993ArgAsn: 1.993 ± 0.061	2.388ArgPro: 2.388 ± 0.059	3.108ArgGln: 3.108 ± 0.073	3.547ArgArg: 3.547 ± 0.077	3.228ArgSer: 3.228 ± 0.069	2.523ArgThr: 2.523 ± 0.071	4.157ArgVal: 4.157 ± 0.072	0.863ArgTrp: 0.863 ± 0.033	2.19ArgTyr: 2.19 ± 0.067	0.0ArgXaa: 0.0 ± 0.0
Ser	6.457SerAla: 6.457 ± 0.096	0.573SerCys: 0.573 ± 0.027	2.91SerAsp: 2.91 ± 0.062	2.992SerGlu: 2.992 ± 0.063	2.212SerPhe: 2.212 ± 0.065	5.147SerGly: 5.147 ± 0.086	1.356SerHis: 1.356 ± 0.048	3.08SerIle: 3.08 ± 0.058	2.339SerLys: 2.339 ± 0.06	6.71SerLeu: 6.71 ± 0.109	1.551SerMet: 1.551 ± 0.045	1.939SerAsn: 1.939 ± 0.051	2.675SerPro: 2.675 ± 0.062	2.545SerGln: 2.545 ± 0.062	3.413SerArg: 3.413 ± 0.064	3.773SerSer: 3.773 ± 0.095	3.084SerThr: 3.084 ± 0.072	4.12SerVal: 4.12 ± 0.085	0.797SerTrp: 0.797 ± 0.034	1.618SerTyr: 1.618 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	5.402ThrAla: 5.402 ± 0.099	0.346ThrCys: 0.346 ± 0.018	2.718ThrAsp: 2.718 ± 0.076	2.687ThrGlu: 2.687 ± 0.067	1.749ThrPhe: 1.749 ± 0.052	4.605ThrGly: 4.605 ± 0.096	1.157ThrHis: 1.157 ± 0.042	2.434ThrIle: 2.434 ± 0.064	1.42ThrLys: 1.42 ± 0.051	6.701ThrLeu: 6.701 ± 0.116	1.079ThrMet: 1.079 ± 0.043	1.364ThrAsn: 1.364 ± 0.05	3.163ThrPro: 3.163 ± 0.061	2.289ThrGln: 2.289 ± 0.055	3.058ThrArg: 3.058 ± 0.062	2.827ThrSer: 2.827 ± 0.064	2.95ThrThr: 2.95 ± 0.085	3.826ThrVal: 3.826 ± 0.089	0.498ThrTrp: 0.498 ± 0.027	1.187ThrTyr: 1.187 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	7.583ValAla: 7.583 ± 0.116	0.702ValCys: 0.702 ± 0.031	4.006ValAsp: 4.006 ± 0.074	4.098ValGlu: 4.098 ± 0.09	2.543ValPhe: 2.543 ± 0.053	4.84ValGly: 4.84 ± 0.079	1.501ValHis: 1.501 ± 0.052	3.965ValIle: 3.965 ± 0.079	2.813ValLys: 2.813 ± 0.074	7.887ValLeu: 7.887 ± 0.131	2.026ValMet: 2.026 ± 0.058	2.535ValAsn: 2.535 ± 0.063	3.151ValPro: 3.151 ± 0.067	2.868ValGln: 2.868 ± 0.068	4.129ValArg: 4.129 ± 0.076	4.626ValSer: 4.626 ± 0.081	4.047ValThr: 4.047 ± 0.105	5.539ValVal: 5.539 ± 0.097	0.872ValTrp: 0.872 ± 0.042	1.819ValTyr: 1.819 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	1.027TrpAla: 1.027 ± 0.037	0.136TrpCys: 0.136 ± 0.014	0.573TrpAsp: 0.573 ± 0.027	0.548TrpGlu: 0.548 ± 0.026	0.494TrpPhe: 0.494 ± 0.027	0.768TrpGly: 0.768 ± 0.036	0.378TrpHis: 0.378 ± 0.022	0.589TrpIle: 0.589 ± 0.034	0.504TrpLys: 0.504 ± 0.024	2.077TrpLeu: 2.077 ± 0.066	0.333TrpMet: 0.333 ± 0.021	0.379TrpAsn: 0.379 ± 0.023	0.509TrpPro: 0.509 ± 0.025	1.065TrpGln: 1.065 ± 0.045	0.921TrpArg: 0.921 ± 0.036	0.648TrpSer: 0.648 ± 0.032	0.526TrpThr: 0.526 ± 0.027	0.886TrpVal: 0.886 ± 0.034	0.222TrpTrp: 0.222 ± 0.02	0.312TrpTyr: 0.312 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.942TyrAla: 2.942 ± 0.064	0.288TyrCys: 0.288 ± 0.019	1.522TyrAsp: 1.522 ± 0.085	1.301TyrGlu: 1.301 ± 0.042	1.111TyrPhe: 1.111 ± 0.041	2.094TyrGly: 2.094 ± 0.051	0.649TyrHis: 0.649 ± 0.03	1.122TyrIle: 1.122 ± 0.039	0.897TyrLys: 0.897 ± 0.033	2.948TyrLeu: 2.948 ± 0.062	0.53TyrMet: 0.53 ± 0.027	0.834TyrAsn: 0.834 ± 0.042	1.218TyrPro: 1.218 ± 0.042	1.467TyrGln: 1.467 ± 0.049	1.908TyrArg: 1.908 ± 0.062	1.566TyrSer: 1.566 ± 0.046	1.416TyrThr: 1.416 ± 0.053	1.849TyrVal: 1.849 ± 0.057	0.406TyrTrp: 0.406 ± 0.027	0.833TyrTyr: 0.833 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2395 proteins (762620 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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