Amino acid dipepetide frequency for Microbacterium phage Pajaza

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.508AlaAla: 8.508 ± 1.023
0.546AlaCys: 0.546 ± 0.261
6.088AlaAsp: 6.088 ± 0.555
5.854AlaGlu: 5.854 ± 0.841
3.122AlaPhe: 3.122 ± 0.5
7.337AlaGly: 7.337 ± 1.381
1.639AlaHis: 1.639 ± 0.367
6.01AlaIle: 6.01 ± 0.917
5.698AlaLys: 5.698 ± 1.001
8.742AlaLeu: 8.742 ± 1.125
2.732AlaMet: 2.732 ± 0.398
2.966AlaAsn: 2.966 ± 0.449
3.356AlaPro: 3.356 ± 0.562
2.966AlaGln: 2.966 ± 0.543
6.244AlaArg: 6.244 ± 0.899
4.761AlaSer: 4.761 ± 0.599
6.556AlaThr: 6.556 ± 0.614
5.464AlaVal: 5.464 ± 0.644
1.561AlaTrp: 1.561 ± 0.346
2.498AlaTyr: 2.498 ± 0.346
0.0AlaXaa: 0.0 ± 0.0
Cys
0.781CysAla: 0.781 ± 0.235
0.078CysCys: 0.078 ± 0.08
0.702CysAsp: 0.702 ± 0.232
0.39CysGlu: 0.39 ± 0.149
0.234CysPhe: 0.234 ± 0.128
0.546CysGly: 0.546 ± 0.237
0.234CysHis: 0.234 ± 0.126
0.078CysIle: 0.078 ± 0.071
0.859CysLys: 0.859 ± 0.314
0.468CysLeu: 0.468 ± 0.17
0.0CysMet: 0.0 ± 0.0
0.078CysAsn: 0.078 ± 0.084
0.468CysPro: 0.468 ± 0.205
0.312CysGln: 0.312 ± 0.153
0.468CysArg: 0.468 ± 0.176
0.234CysSer: 0.234 ± 0.129
0.546CysThr: 0.546 ± 0.248
0.312CysVal: 0.312 ± 0.139
0.156CysTrp: 0.156 ± 0.096
0.156CysTyr: 0.156 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
4.605AspAla: 4.605 ± 0.676
0.546AspCys: 0.546 ± 0.197
6.166AspAsp: 6.166 ± 1.202
5.386AspGlu: 5.386 ± 1.225
2.42AspPhe: 2.42 ± 0.402
4.995AspGly: 4.995 ± 0.552
1.639AspHis: 1.639 ± 0.381
3.668AspIle: 3.668 ± 0.557
2.264AspLys: 2.264 ± 0.378
5.62AspLeu: 5.62 ± 0.616
1.639AspMet: 1.639 ± 0.383
2.107AspAsn: 2.107 ± 0.39
3.981AspPro: 3.981 ± 0.713
2.42AspGln: 2.42 ± 0.379
3.434AspArg: 3.434 ± 0.571
4.059AspSer: 4.059 ± 0.56
3.981AspThr: 3.981 ± 0.673
3.981AspVal: 3.981 ± 0.454
1.561AspTrp: 1.561 ± 0.377
2.498AspTyr: 2.498 ± 0.426
0.0AspXaa: 0.0 ± 0.0
Glu
7.181GluAla: 7.181 ± 1.065
0.39GluCys: 0.39 ± 0.223
5.698GluAsp: 5.698 ± 1.089
5.386GluGlu: 5.386 ± 1.214
1.951GluPhe: 1.951 ± 0.474
5.073GluGly: 5.073 ± 0.498
1.015GluHis: 1.015 ± 0.253
2.498GluIle: 2.498 ± 0.427
2.732GluLys: 2.732 ± 0.478
6.478GluLeu: 6.478 ± 0.783
2.185GluMet: 2.185 ± 0.403
2.498GluAsn: 2.498 ± 0.351
2.029GluPro: 2.029 ± 0.399
1.639GluGln: 1.639 ± 0.297
3.356GluArg: 3.356 ± 0.634
2.966GluSer: 2.966 ± 0.531
4.215GluThr: 4.215 ± 0.49
4.527GluVal: 4.527 ± 0.708
1.873GluTrp: 1.873 ± 0.385
2.029GluTyr: 2.029 ± 0.421
0.0GluXaa: 0.0 ± 0.0
Phe
2.576PheAla: 2.576 ± 0.394
0.312PheCys: 0.312 ± 0.155
1.873PheAsp: 1.873 ± 0.336
1.327PheGlu: 1.327 ± 0.275
0.781PhePhe: 0.781 ± 0.25
2.342PheGly: 2.342 ± 0.458
0.312PheHis: 0.312 ± 0.149
1.327PheIle: 1.327 ± 0.3
1.561PheLys: 1.561 ± 0.345
2.576PheLeu: 2.576 ± 0.422
0.702PheMet: 0.702 ± 0.263
1.405PheAsn: 1.405 ± 0.297
1.561PhePro: 1.561 ± 0.29
1.405PheGln: 1.405 ± 0.34
2.342PheArg: 2.342 ± 0.363
2.42PheSer: 2.42 ± 0.469
2.185PheThr: 2.185 ± 0.532
1.873PheVal: 1.873 ± 0.401
0.39PheTrp: 0.39 ± 0.218
0.702PheTyr: 0.702 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
6.478GlyAla: 6.478 ± 1.154
0.624GlyCys: 0.624 ± 0.212
4.839GlyAsp: 4.839 ± 0.574
4.215GlyGlu: 4.215 ± 0.472
2.107GlyPhe: 2.107 ± 0.388
6.01GlyGly: 6.01 ± 0.849
1.483GlyHis: 1.483 ± 0.312
5.151GlyIle: 5.151 ± 0.793
3.434GlyLys: 3.434 ± 0.621
6.478GlyLeu: 6.478 ± 1.051
2.029GlyMet: 2.029 ± 0.295
2.732GlyAsn: 2.732 ± 0.458
2.029GlyPro: 2.029 ± 0.41
3.356GlyGln: 3.356 ± 0.636
4.605GlyArg: 4.605 ± 0.571
5.854GlySer: 5.854 ± 0.75
5.464GlyThr: 5.464 ± 0.69
6.244GlyVal: 6.244 ± 0.671
1.561GlyTrp: 1.561 ± 0.357
2.42GlyTyr: 2.42 ± 0.457
0.0GlyXaa: 0.0 ± 0.0
His
0.702HisAla: 0.702 ± 0.287
0.234HisCys: 0.234 ± 0.147
1.015HisAsp: 1.015 ± 0.274
1.171HisGlu: 1.171 ± 0.417
0.468HisPhe: 0.468 ± 0.188
1.483HisGly: 1.483 ± 0.355
0.546HisHis: 0.546 ± 0.214
1.639HisIle: 1.639 ± 0.402
0.624HisLys: 0.624 ± 0.28
1.327HisLeu: 1.327 ± 0.351
0.468HisMet: 0.468 ± 0.177
0.468HisAsn: 0.468 ± 0.21
1.093HisPro: 1.093 ± 0.344
0.546HisGln: 0.546 ± 0.207
0.468HisArg: 0.468 ± 0.208
0.937HisSer: 0.937 ± 0.233
0.702HisThr: 0.702 ± 0.188
1.093HisVal: 1.093 ± 0.369
0.702HisTrp: 0.702 ± 0.216
0.702HisTyr: 0.702 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
5.542IleAla: 5.542 ± 0.691
0.312IleCys: 0.312 ± 0.159
3.825IleAsp: 3.825 ± 0.471
3.2IleGlu: 3.2 ± 0.564
0.781IlePhe: 0.781 ± 0.256
3.356IleGly: 3.356 ± 0.886
0.702IleHis: 0.702 ± 0.237
3.512IleIle: 3.512 ± 0.736
2.732IleLys: 2.732 ± 0.47
4.059IleLeu: 4.059 ± 0.582
1.717IleMet: 1.717 ± 0.342
1.717IleAsn: 1.717 ± 0.352
2.732IlePro: 2.732 ± 0.594
2.81IleGln: 2.81 ± 0.682
2.42IleArg: 2.42 ± 0.5
3.356IleSer: 3.356 ± 0.549
3.278IleThr: 3.278 ± 0.615
3.512IleVal: 3.512 ± 0.598
0.702IleTrp: 0.702 ± 0.243
1.873IleTyr: 1.873 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
5.229LysAla: 5.229 ± 0.846
0.312LysCys: 0.312 ± 0.187
2.342LysAsp: 2.342 ± 0.536
3.278LysGlu: 3.278 ± 0.453
1.405LysPhe: 1.405 ± 0.287
3.981LysGly: 3.981 ± 0.507
0.546LysHis: 0.546 ± 0.201
1.717LysIle: 1.717 ± 0.356
3.434LysLys: 3.434 ± 0.76
2.107LysLeu: 2.107 ± 0.535
1.483LysMet: 1.483 ± 0.341
2.107LysAsn: 2.107 ± 0.389
2.966LysPro: 2.966 ± 0.582
1.717LysGln: 1.717 ± 0.336
2.81LysArg: 2.81 ± 0.538
2.888LysSer: 2.888 ± 0.514
4.059LysThr: 4.059 ± 0.678
3.903LysVal: 3.903 ± 0.545
0.702LysTrp: 0.702 ± 0.248
1.483LysTyr: 1.483 ± 0.279
0.0LysXaa: 0.0 ± 0.0
Leu
8.039LeuAla: 8.039 ± 0.95
0.702LeuCys: 0.702 ± 0.226
5.62LeuAsp: 5.62 ± 0.643
5.542LeuGlu: 5.542 ± 0.787
2.42LeuPhe: 2.42 ± 0.373
6.634LeuGly: 6.634 ± 0.849
1.093LeuHis: 1.093 ± 0.305
3.668LeuIle: 3.668 ± 0.816
3.668LeuLys: 3.668 ± 0.568
6.166LeuLeu: 6.166 ± 0.841
1.561LeuMet: 1.561 ± 0.344
2.888LeuAsn: 2.888 ± 0.48
4.995LeuPro: 4.995 ± 0.824
2.654LeuGln: 2.654 ± 0.515
4.839LeuArg: 4.839 ± 0.612
6.01LeuSer: 6.01 ± 0.698
7.493LeuThr: 7.493 ± 1.016
7.337LeuVal: 7.337 ± 0.81
1.483LeuTrp: 1.483 ± 0.309
2.264LeuTyr: 2.264 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
3.59MetAla: 3.59 ± 0.554
0.156MetCys: 0.156 ± 0.113
1.405MetAsp: 1.405 ± 0.366
1.171MetGlu: 1.171 ± 0.317
0.312MetPhe: 0.312 ± 0.145
2.029MetGly: 2.029 ± 0.404
0.312MetHis: 0.312 ± 0.158
0.859MetIle: 0.859 ± 0.221
1.015MetLys: 1.015 ± 0.225
1.717MetLeu: 1.717 ± 0.389
0.781MetMet: 0.781 ± 0.191
0.859MetAsn: 0.859 ± 0.245
1.717MetPro: 1.717 ± 0.391
1.171MetGln: 1.171 ± 0.289
1.015MetArg: 1.015 ± 0.276
2.264MetSer: 2.264 ± 0.462
1.561MetThr: 1.561 ± 0.33
2.264MetVal: 2.264 ± 0.448
0.39MetTrp: 0.39 ± 0.164
0.39MetTyr: 0.39 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
2.888AsnAla: 2.888 ± 0.427
0.234AsnCys: 0.234 ± 0.123
2.185AsnAsp: 2.185 ± 0.456
2.654AsnGlu: 2.654 ± 0.45
0.624AsnPhe: 0.624 ± 0.204
3.356AsnGly: 3.356 ± 0.464
0.546AsnHis: 0.546 ± 0.201
1.483AsnIle: 1.483 ± 0.295
1.717AsnLys: 1.717 ± 0.345
3.59AsnLeu: 3.59 ± 0.577
0.624AsnMet: 0.624 ± 0.187
0.859AsnAsn: 0.859 ± 0.241
2.81AsnPro: 2.81 ± 0.51
1.327AsnGln: 1.327 ± 0.325
1.405AsnArg: 1.405 ± 0.302
2.42AsnSer: 2.42 ± 0.428
1.639AsnThr: 1.639 ± 0.374
2.264AsnVal: 2.264 ± 0.523
0.781AsnTrp: 0.781 ± 0.286
1.249AsnTyr: 1.249 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
4.917ProAla: 4.917 ± 0.854
0.312ProCys: 0.312 ± 0.183
2.576ProAsp: 2.576 ± 0.503
3.278ProGlu: 3.278 ± 0.554
1.951ProPhe: 1.951 ± 0.386
4.059ProGly: 4.059 ± 0.643
0.859ProHis: 0.859 ± 0.251
1.873ProIle: 1.873 ± 0.308
2.498ProLys: 2.498 ± 0.457
3.746ProLeu: 3.746 ± 0.482
0.624ProMet: 0.624 ± 0.25
2.107ProAsn: 2.107 ± 0.45
2.264ProPro: 2.264 ± 0.372
2.576ProGln: 2.576 ± 0.666
2.576ProArg: 2.576 ± 0.534
3.044ProSer: 3.044 ± 0.387
3.356ProThr: 3.356 ± 0.496
4.449ProVal: 4.449 ± 0.544
0.781ProTrp: 0.781 ± 0.233
1.327ProTyr: 1.327 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
4.839GlnAla: 4.839 ± 0.706
0.156GlnCys: 0.156 ± 0.105
1.873GlnAsp: 1.873 ± 0.361
3.2GlnGlu: 3.2 ± 0.514
0.702GlnPhe: 0.702 ± 0.203
1.873GlnGly: 1.873 ± 0.361
0.781GlnHis: 0.781 ± 0.261
2.888GlnIle: 2.888 ± 0.666
2.029GlnLys: 2.029 ± 0.49
3.512GlnLeu: 3.512 ± 0.793
0.702GlnMet: 0.702 ± 0.25
1.483GlnAsn: 1.483 ± 0.36
0.859GlnPro: 0.859 ± 0.301
1.639GlnGln: 1.639 ± 0.351
2.342GlnArg: 2.342 ± 0.433
2.498GlnSer: 2.498 ± 0.396
2.107GlnThr: 2.107 ± 0.422
2.498GlnVal: 2.498 ± 0.454
0.624GlnTrp: 0.624 ± 0.206
1.171GlnTyr: 1.171 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
4.059ArgAla: 4.059 ± 0.566
0.234ArgCys: 0.234 ± 0.143
3.2ArgAsp: 3.2 ± 0.529
3.59ArgGlu: 3.59 ± 0.486
2.498ArgPhe: 2.498 ± 0.421
3.356ArgGly: 3.356 ± 0.528
0.702ArgHis: 0.702 ± 0.245
2.966ArgIle: 2.966 ± 0.404
2.888ArgLys: 2.888 ± 0.596
6.01ArgLeu: 6.01 ± 0.892
1.717ArgMet: 1.717 ± 0.324
1.717ArgAsn: 1.717 ± 0.34
2.81ArgPro: 2.81 ± 0.499
1.561ArgGln: 1.561 ± 0.389
3.2ArgArg: 3.2 ± 0.564
3.512ArgSer: 3.512 ± 0.472
3.2ArgThr: 3.2 ± 0.527
4.605ArgVal: 4.605 ± 0.668
1.561ArgTrp: 1.561 ± 0.385
2.107ArgTyr: 2.107 ± 0.442
0.0ArgXaa: 0.0 ± 0.0
Ser
6.088SerAla: 6.088 ± 0.755
0.312SerCys: 0.312 ± 0.157
4.371SerAsp: 4.371 ± 0.624
3.434SerGlu: 3.434 ± 0.589
2.498SerPhe: 2.498 ± 0.486
6.01SerGly: 6.01 ± 0.711
0.702SerHis: 0.702 ± 0.234
2.498SerIle: 2.498 ± 0.395
3.122SerLys: 3.122 ± 0.612
5.698SerLeu: 5.698 ± 0.584
1.561SerMet: 1.561 ± 0.347
1.717SerAsn: 1.717 ± 0.405
3.122SerPro: 3.122 ± 0.531
3.356SerGln: 3.356 ± 0.537
3.825SerArg: 3.825 ± 0.564
4.215SerSer: 4.215 ± 0.617
3.825SerThr: 3.825 ± 0.628
4.059SerVal: 4.059 ± 0.627
1.639SerTrp: 1.639 ± 0.314
2.185SerTyr: 2.185 ± 0.375
0.0SerXaa: 0.0 ± 0.0
Thr
4.995ThrAla: 4.995 ± 0.73
0.234ThrCys: 0.234 ± 0.182
4.605ThrAsp: 4.605 ± 0.662
3.903ThrGlu: 3.903 ± 0.609
1.951ThrPhe: 1.951 ± 0.465
5.464ThrGly: 5.464 ± 0.588
1.171ThrHis: 1.171 ± 0.375
3.2ThrIle: 3.2 ± 0.535
3.278ThrLys: 3.278 ± 0.602
5.698ThrLeu: 5.698 ± 0.666
1.171ThrMet: 1.171 ± 0.3
1.951ThrAsn: 1.951 ± 0.332
4.839ThrPro: 4.839 ± 0.695
2.029ThrGln: 2.029 ± 0.44
4.137ThrArg: 4.137 ± 0.619
4.449ThrSer: 4.449 ± 0.512
4.059ThrThr: 4.059 ± 0.732
5.229ThrVal: 5.229 ± 0.628
1.873ThrTrp: 1.873 ± 0.405
2.342ThrTyr: 2.342 ± 0.565
0.0ThrXaa: 0.0 ± 0.0
Val
7.727ValAla: 7.727 ± 0.822
0.468ValCys: 0.468 ± 0.168
5.308ValAsp: 5.308 ± 0.679
4.371ValGlu: 4.371 ± 0.618
2.185ValPhe: 2.185 ± 0.485
4.917ValGly: 4.917 ± 0.941
0.937ValHis: 0.937 ± 0.248
4.449ValIle: 4.449 ± 0.62
2.654ValLys: 2.654 ± 0.437
6.088ValLeu: 6.088 ± 0.663
1.717ValMet: 1.717 ± 0.374
2.732ValAsn: 2.732 ± 0.442
3.434ValPro: 3.434 ± 0.439
2.966ValGln: 2.966 ± 0.397
3.2ValArg: 3.2 ± 0.437
4.605ValSer: 4.605 ± 0.528
5.464ValThr: 5.464 ± 0.754
5.229ValVal: 5.229 ± 0.897
1.717ValTrp: 1.717 ± 0.329
2.107ValTyr: 2.107 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
1.483TrpAla: 1.483 ± 0.355
0.39TrpCys: 0.39 ± 0.161
1.405TrpAsp: 1.405 ± 0.285
1.405TrpGlu: 1.405 ± 0.314
0.781TrpPhe: 0.781 ± 0.238
1.639TrpGly: 1.639 ± 0.442
0.624TrpHis: 0.624 ± 0.23
1.015TrpIle: 1.015 ± 0.234
0.624TrpLys: 0.624 ± 0.219
2.185TrpLeu: 2.185 ± 0.481
0.39TrpMet: 0.39 ± 0.171
0.937TrpAsn: 0.937 ± 0.228
0.859TrpPro: 0.859 ± 0.272
0.702TrpGln: 0.702 ± 0.252
1.171TrpArg: 1.171 ± 0.303
1.483TrpSer: 1.483 ± 0.439
1.483TrpThr: 1.483 ± 0.337
1.795TrpVal: 1.795 ± 0.372
0.546TrpTrp: 0.546 ± 0.255
0.781TrpTyr: 0.781 ± 0.256
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.42TyrAla: 2.42 ± 0.443
0.546TyrCys: 0.546 ± 0.202
1.873TyrAsp: 1.873 ± 0.4
2.966TyrGlu: 2.966 ± 0.467
0.859TyrPhe: 0.859 ± 0.236
2.654TyrGly: 2.654 ± 0.445
0.546TyrHis: 0.546 ± 0.23
1.639TyrIle: 1.639 ± 0.453
1.639TyrLys: 1.639 ± 0.428
2.81TyrLeu: 2.81 ± 0.454
1.093TyrMet: 1.093 ± 0.251
1.327TyrAsn: 1.327 ± 0.297
1.249TyrPro: 1.249 ± 0.355
0.624TyrGln: 0.624 ± 0.196
1.639TyrArg: 1.639 ± 0.387
2.264TyrSer: 2.264 ± 0.447
1.405TyrThr: 1.405 ± 0.319
1.639TyrVal: 1.639 ± 0.402
1.093TyrTrp: 1.093 ± 0.259
1.249TyrTyr: 1.249 ± 0.351
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (12813 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski